H. pylori 제균 실패율과 clarithromycin 내성률의 일치성

허재형 ( J. H. Heo ),남승우 ( S. W. Nam ),노임환 ( I. H. Roe ),양미라 ( M. R. Yang ),김정택 ( J. T. Kim ),송일환 ( I. H. Song ),임창영 ( C. Y. Lim ),김정원 ( J. W. Kim ),신지현 ( J. H. Shin ) 대한소화기학회 2002 대한소화기학회 춘계학술대회 Vol.2002 No.-

<목적> H. pylori 제균 치료성적을 좌우하는 요소에는 약제와 대상 환자군의 선정, 균검사방법의 차이, 항생제 저항성 등이 중요시되고 있다. 이 중에서도 항생제 저항성은 나라간의 H. pylori 제균 성적을 다르게 하는 대표적인 원인이다. 우리나라는 제균률이 외국보다 저조하여 85%내외로 보고되고 있다. 본 연구의 목적은 H. pylori 제균 실패율과 clarithromycin 내성률을 조사하여 제균 실패 원인으로서의 clarithromycin

The clonal origins of leukemic progression of myelodysplasia

Kim, T,Tyndel, M S,Kim, H J,Ahn, J-S,Choi, S H,Park, H J,Kim, Y-k,Yang, D-H,Lee, J-J,Jung, S-H,Kim, S Y,Min, Y H,Cheong, J-W,Sohn, S K,Moon, J H,Choi, M,Lee, M,Zhang, Z,Kim, D(D H) Nature Publishing Group 2017 Leukemia Vol.31 No.9

<P>The genetics behind the progression of myelodysplasia to secondary acute myeloid leukemia (sAML) is poorly understood. In this study, we profiled somatic mutations and their dynamics using next generation sequencing on serial samples from a total of 124 patients, consisting of a 31 patient discovery cohort and 93 patients from two validation cohorts. Whole-exome analysis on the discovery cohort revealed that 29 of 31 patients carry mutations related to at least one of eight commonly mutated pathways in AML. Mutations in genes related to DNA methylation and splicing machinery were found in T-cell samples, which expand at the initial diagnosis of the myelodysplasia, suggesting their importance as early disease events. On the other hand, somatic variants associated with signaling pathways arise or their allelic burdens expand significantly during progression. Our results indicate a strong association between mutations in activated signaling pathways and sAML progression. Overall, we demonstrate that distinct categories of genetic lesions play roles at different stages of sAML in a generally fixed order.</P>

Comparison of Single and Blend Acidifiers as Alternative to Antibiotics on Growth Performance, Fecal Microflora, and Humoral Immunity in Weaned Piglets

Ahmed, S.T.,Hwang, J.A.,Hoon, J.,Mun, H.S.,Yang, C.J. Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.1

The banning of the use of antibiotics as feed additive has accelerated investigations of alternative feed additives in animal production. This experiment investigated the effect of pure citric acid or acidifier blend supplementation as substitute for antibiotic growth promoters on growth performance, fecal microbial count, and humoral immunity in weaned piglets challenged with Salmonella enterica serover Typhimurium and Escherichia coli KCTC 2571. A total of 60 newly weaned piglets (crossbred, 28-d-old; average 8 kg initial weight) were randomly assigned to four dietary treatments in a completely randomized design. Dietary treatments included NC (negative control; basal diet), PC (positive control; basal diet+0.002% apramycin), T1 (basal diet+0.5% pure citric acid), and T2 (basal diet+0.4% acidifier blend). All piglets were orally challenged with 5 mL of culture fluid containing $2.3{\times}10^8$ cfu/mL of E. coli KCTC 2571 and $5.9{\times}10^8$ cfu/mL of S. typhimurium at the beginning of the experiment. The PC group showed the highest ADG and ADFI, whereas gain:feed was improved in the PC and T1 group (p<0.05). All dietary treatments showed significant reduction in fecal counts of Salmonella and E. coli, compared to NC (p<0.05), with PC being better than T1 and T2. Significant elevation in fecal Lactobacillus spp. counts was shown by treatments with T1, T2, and PC, whereas Bacillus spp. counts were increased by treatment with T1 and T2 compared to NC and PC diet (p<0.05). Serum IgG concentration was increased by T1 diet (p<0.05), whereas IgM and IgA were not significantly affected by any of the dietary treatments (p>0.05). From these above results, it can be concluded that, as alternatives to antibiotics dietary acidification with pure citric acid or acidifiers blend did not fully ameliorate the negative effects of microbial challenges in respect of growth performance and microbial environment, however improved immunity suggested further research with different dose levels.

돈 분뇨 액비에 미생물 첨가가 배추의 발아지수에 미치는 영향

김태일,유용희,정의수,양창범,김민균,Kim, T. I.,Yoo, Y. H.,Chung, E. S.,Barroga, Antonio J.,Yang, C. B.,Kim, M. K. 한국축산환경학회 2005 축산시설환경학회지 Vol.11 No.2

본 연구는 돈 분뇨 액비에 미생물 첨가가 배추의 발아지수에 미치는 효과를 구명하기 위해서 수행하였다. 돈 분뇨 액비는 107일 동안 폭기와 무폭기 상태로 액상비료를 제조하여 공시하였고 미생물 첨가는 $0.05\%,\;0.1\%,\;0.2\%,\;0.3\%$ 수준으로 하였다. $0.05\%$ 미생물 접종 처리구에서 식물 독성이 낮고 발아지수가 높게 나타났다. 첨가수준별 돈분 액비 이용시험에서 초기 돈 슬러리의 액비성상은 T-N 2,873 ppm, T-P 753 ppm, $NH_4-N$ 1,441.6 ppm, $NO_3-N$ 16.48ppm으로 50일이 되면서 폭기조건에서 T-N 3,672 ppm, T-P 164 ppm, $NH_4-N$ 183.87 ppm, $NO_3-N$ 21.97 ppm로 변화하였으며, 무폭기 조건에서 T-N 1,261 ppm, T-P 68 ppm, $NH_4-N$ 161 ppm, $NO_3-N$ 16.87 ppm로 나타났다. 미생물을 처리하지 않고 107일동안 폭기조건과 무폭기 조건으로 액비화한 시료의 발아지수는 각각 83과 40.4이였다. 돈 분뇨 액비를 무산소 제조시 $40\%$ 이상, 호기제조시 $50\%$ 이상의 발아지수가 개선되었다. 이와 같은 결과는 액비제조 처리가 무산소 제조보다는 호기제조 처리가 작물에 양호한 것으로 판단되어지며 이는 호기처리시 총질소의 함량을 높게 유지할 수 있도록 하는 결과인 것으로 사료된다. This study was carried out to investigate the effect of Synechocystis sp. KACC 91007 when added to a pig slurry or pig liquid fertilizer (PLF) on germination index (GI) of Chinese cabbage. The preliminary experiment involved the screening of inoculant levels which were; 0.05, 0.1, 0.2, and $0.3\%$, respectively. The $0.05\%$ level of inoculant was selected based on low phytotoxicity and high GI.. The PLF underwent a 107 day aerobic and anoxic processing conditions. The T-N, T-P, $NH_4$, and $NO_3-N$ concentrations of the untreated pig slurry were; 2,873, 753, 1,441.6, and 16.48 ppm, respectively. Using aerobic processing treatment, the fertilizer value of the PLF was 3,672, 164, 183.87, and 21.97 ppm, respectively. In contrast, the fertilizer value of the PLF processed under anoxic condition was reduced to 1,261, 68, 161, and 16.87 ppm. The GI value of the untreated PLF under aerobic and anoxic processing condition was 83 and $40.4^{*}\%$, respectively. With the addition of the $0.05\%$ microbial inoculant, the GI improved by more than 40 and $50\%$ respectively, when the PLF was processed under anoxic and aerobic conditions. The above findings proved that the aerobic processing of PLF for 107 days was better than anoxic and yielded higher T-N which is a macro-nutrient fertilizer material. Consequently, the addition of $0.05\%$ microbial inoculant resulted to a higher GI of the Chinese cabbage specifically under aerobic processing condition.

사료급여방법이 제주재래마의 체성장 및 도체품질에 미치는 영향

오운용(W . Y . Oh),강태홍(T . H . Kang),김동철(D . C . Kim),진신흠(S . H . Jin),홍성귀(S . K . Hong),양승주(S . J . Yang),정재홍(J . H . Jung) 한국축산학회 1993 한국축산학회지 Vol.35 No.6

Nine Cheju Native Horses with an average weight of 169.2㎏ were used in a 238-d (34-wk) fattening trial designed to evaluate the effects of feeding method on growth performance and carcass characteristics. Horses were randomly alloted to three treatment groups: T₁ 6-wk (Former fattening period: FFP) + 28-wk (Latter fattening period: LFP), T₂, 9-wk (FFP) + 25-wk (LFP), T₃, 12-wk (FFP) + 22-wk (LFP). During the FFP, concentrate was restricted at approximately l.5% of their body weights but horses were allowed ad libitum access to concentrate during the subsequent LFP. The horses were individually penned in 2.5-m×3-m pipe stalls with front feeding gates. They were allowed 3 h of exercise in a pen (50-m×30-m) outside the barn monthly. Horses were weighed at 30-d intervals and feed consumption was measured daily. At the end of the 238-d fattening period, one horse in each treatment was selected randomly and slaughtered for meat quality evaluation. Results obtained from the present study were summarized as follows; during the overall fattening period, average daily gains (ADG) and nutrient intake (TDN) per ㎏ wt, gain were 0.59, 5.10(T₁), 0.51, 5.84(T₂) and 0.43, 6.37㎏(T₂), respectively. When horses were allowed ad libitum access to concentrate for 28 wk(T₁), ADG and feed efficiency(TDN) were improved by 37%(P$gt;.05) and20%(P$gt;.05)compared to those allowed concentrate free choice for 22 wk(T₃). Horse in T, group had an improved dressing percentage and carcass weight with good quality grades(intramuscular fat, meat color, fat color). Taste panelists(150 amateures) detected no difference from longissimus lumborum steaks between Cheju Native Horse and improved Cheju Native Cattle, but found juiciness and meat flavor were superior to those of beef.

홀스타인 육성우의 계절별 분뇨 배설량 및 특성에 관한 연구

최동윤,곽정훈,박치호,정광화,김태일,김재환,유용희,양창범,최홍림,Choi, D.Y.,Kwag, J.H.,Park, C.H.,Jeong, K.H.,Kim, T.I.,Kim, J.H.,Yoo, Y.H.,Yang, C.B.,Hong, H.L. 한국축산환경학회 2006 축산시설환경학회지 Vol.12 No.3

우리나라에서 사육되고 있는 젖소 육성우의 분뇨배설량을 구명하기 위하여 육성우 6두를 공시하여 축산연구소 낙농우사에서 봄, 여름, 가을, 겨울 등 4계절에 걸쳐 각각 10일간 시험을 수행하였으며, 그 결과는 다음과 같다. 1. 건물섭취량은 평균 6.7kg으로 봄, 여름, 가을 및 겨울이 각각 4.6, 8.3, 7.1, 6.8kg으로 봄이 가장 낮은 섭취량을 보였으며, 음수량은 평균 $19.3{\ell}$로, 여름이 $21.8{\ell}$로 가장 많았고 봄과 겨울이 $18.3{\ell}$로 낮았으나 계절별 유의차는 보이지 않았다. 2. 분뇨배설량을 계절별로 조사한 결과, 봄 13.7kg, 여름 23.8kg, 가을 25.0kg, 겨울 20.2kg으로 봄이 가장 낮았으며(p<0.05), 전체 평균 분뇨배설량은 20.3kg이였고, 체중대비 분뇨의 비율은 5.6%였다. 3. 체중과 분뇨배설량간($R^2=0.7816$), 체중과 사료섭취량간($R^2=0.7296$)의 상관관계가 비교적 높았으나, 음수량과 분뇨배설량, 사료섭취량과 분뇨배설량간의 상관관계는 비교적 낮았다. 4. 분뇨의 수분 및 pH를 분석해 본 결과, 분의 뇨의 평균 수분 함량은 각각 83.5, 94.6%였으며, pH는 각각 7.4, 7.6 수준이었다. 5. 분뇨중 오염물질 농도는 분의 $BOD_5,\;COD_{Mn}$, SS, T-N 및 T-P는 각각 18,048, 50,114, 119,833, 2,519, $427mg/{\ell}$였고, 뇨는 각각 5,434, 6,550, 825, 3,616, $28mg/{\ell}$였다. 6. 분뇨중 비료성분은 분의 N, $P_2O_5,\;K_2O$가 각각 0.25, 0.10, 0.14%였고, 뇨는 각각 0.36, 0.006, 0.31%였다.도는 3m 지점에서 $446.6\;{\mu}g/m^3$ 이었으나 20 m 지점에서는 $156.3\;{\mu}g/m^3$로 34.9% 수준이었고 PM10 34.9%, PM2.5 48.7%, PM1.0 86.8% 수준이었다. This study was conducted to determine the volume of Holstein heifers manure excreted and its characteristics. The average dry matter intake of heifers was 6.7 kg/head/day. The intake rate was lowest in spring among four seasons. The average dry matter intake rate during spring, summer, fall, and winter was 4.6, 8.3, 7.1, and 6.8 kg/head/day, respectively. The average water intake of heifers was $19.3{\ell}/head/day$. The wale. consumption was highest value ($21.8{\ell}/head/day$) in summer and lowest values ($18.3{\ell}/head/day$) in spring and winter. Values were found not to be statiscally different for the four seasons. The average manure production of heifers (average live weight was 363.1 kg) was 20.3 kg/head/day and it was 5.6% of live animal weight. The manure production during spring, summer, fall, and winter was 13.7, 23.5, 25.0, and 20.2 kg/head/day, respectively. Production during spring was lower than the other seasons (p<0.05). A higher correlation between live weight and manure production ($R^2=0.7816$) and between live weight and feed intake ($R^2=0.7296$) was observed for heifers. Correlations between manure production and water intake and between manure production and feed intake were found to be relatively low for heifers. The moisture content of feces was 83.5% and that of urine 94.6%. The pH of feces and urine were in the ranges of 7.4 and 7.5, respectively. The $BOD_5$, COD, SS, T-N, T-P concentrations of the heifer feces were 18,048, 50,114, 119,833, 2,519, and $427mg/{\ell}$, respectively. Heifer urine showed lower levels of $BOD_5(5,434mg/{\ell})$, COD$(6,550mg/{\ell})$, SS$(825mg/{\ell})$, T-N$(3,616mg/{\ell})$, and $T-P(28mg/{\ell})$ than feces. The fertilizer nutrient concentrations of heifer feces was 0.25% N, 0.1% $P_2O_5$ and 0.14% $K_2O$. Urine was found to contain 0.36% N, 0.006% of $P_2O_5$ and 0.31% $K_2O$.

Axial behaviour of reinforced concrete short columns strengthened with wire rope and T-shaped steel plate units

Yang, K.-H.,Ashour, A.F.,Lee, E.-T. THOMAS TELFORD SERVICES LTD 2009 Magazine of concrete research Vol.61 No.2

Perturbative studies of toroidal momentum transport in KSTAR H-mode and the effect of ion temperature perturbation

Yang, S.M.,Na, Yong-Su,Na, D.H.,Park, J.-K.,Shi, Y.J.,Ko, W.H.,Lee, S.G.,Hahm, T.S. International Atomic Energy Agency 2018 Nuclear fusion Vol.58 No.6

<P>Perturbative experiments have been carried out using tangential neutral beam injection (NBI) and non-resonant magnetic perturbation (NRMP) to analyze the momentum transport properties in KSTAR H-modes. Diffusive and non-diffusive terms of momentum transport are evaluated from the transient analysis. Although the operating conditions and methodologies applied in the two cases are similar, the momentum transport properties obtained show clear differences. The estimated momentum diffusivity and pinch obtained in the NBI modulation experiments is larger than that in the NRMP modulation experiments. We found that this discrepancy could be a result of uncertainties in the assumption for the analysis. By introducing time varying momentum transport coefficients depending on the temperature gradient, the linearized equation shows that if the temperature perturbation exists, the evolution of toroidal rotation perturbation could be faster than the transport rate of mean quantity, since the evolution of toroidal rotation perturbation is related to <img ALIGN='MIDDLE' ALT='' SRC='http://ej.iop.org/images/0029-5515/58/6/066008/nfaab90eieqn001.gif'/>, a momentum diffusivity from perturbative analysis. This could explain the estimated higher momentum diffusivity using time independent transport coefficients in NBI experiments with higher ion temperature perturbation compared to that in NRMP modulation experiments. The differences in the momentum transport coefficient with NRMP and NBI are much reduced by considering time varying momentum transport coefficients in the time dependent transport simulation.</P>

t-SNE 기법을 적용한 지중송전케이블 시스템 PD Pulse 분석

정연하(JUNG Y.H.),장태인(JANG T.I.),최민희(CHOI M.H.),이의찬(LEE E.C.),권대혁(Kwon D.H),양희석(YANG H.S),고재홍(GO J.H.),차상국(CHA S.K),황광모(HWANG G.M.),김숙주(KIM S.J.) 대한전기학회 2020 대한전기학회 학술대회 논문집 Vol.2020 No.7

Cooperative Activation of PI3K by Ras and Rho Family Small GTPases

Yang, H.,Shin, M.G.,Lee, S.,Kim, J.R.,Park, W.,Cho, K.H.,Meyer, T.,Do Heo, W. Cell Press 2012 Molecular cell Vol.47 No.2

Phosphoinositide 3-kinases (PI3Ks) and Ras and Rho family small GTPases are key regulators of cell polarization, motility, and chemotaxis. They influence each other's activities by direct and indirect feedback processes that are only partially understood. Here, we show that 21 small GTPase homologs activate PI3K. Using a microscopy-based binding assay, we show that K-Ras, H-Ras, and five homologous Ras family small GTPases function upstream of PI3K by directly binding the PI3K catalytic subunit, p110. In contrast, several Rho family small GTPases activated PI3K by an indirect cooperative positive feedback that required a combination of Rac, CDC42, and RhoG small GTPase activities. Thus, a distributed network of Ras and Rho family small GTPases induces and reinforces PI3K activity, explaining past challenges to elucidate the specific relevance of different small GTPases in regulating PI3K and controlling cell polarization and chemotaxis.