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Opportunities for microfluidic technologies in synthetic biology
Gulati, Shelly,Rouilly, Vincent,Niu, Xize,Chappell, James,Kitney, Richard I.,Edel, Joshua B.,Freemont, Paul S.,deMello, Andrew J. The Royal Society 2009 Journal of the Royal Society, Interface Vol.6 No.suppl4
<P>We introduce microfluidics technologies as a key foundational technology for synthetic biology experimentation. Recent advances in the field of microfluidics are reviewed and the potential of such a technological platform to support the rapid development of synthetic biology solutions is discussed.</P>
Titania nanopores with dual micro-/nano-topography for selective cellular bioactivity
Gulati, Karan,Moon, Ho-Jin,Li, Tao,Sudheesh Kumar, P.T.,Ivanovski, Saš,o Elsevier 2018 Materials Science and Engineering C Vol.91 No.-
<P><B>Abstract</B></P> <P>This letter describes a simple surface modification strategy based on a single-step electrochemical anodization towards generating dual micro- and nano-rough horizontally-aligned TiO<SUB>2</SUB> nanopores on the surface of clinically utilized micro-grooved titanium implants. Primary macrophages, osteoblasts and fibroblasts were cultured on the nano-engineered implants, and it was demonstrated that the modified surfaces selectively reduced the proliferation of macrophages (immunomodulation), while augmenting the activity of osteoblasts (osseo-integration) and fibroblasts (soft-tissue integration). Additionally, the mechanically robust nanopores also stimulated osteoblast and fibroblast adhesion, attachment and alignment along the direction of the pores/grooves, while macrophages remained oval-shaped and sparsely distributed. This study for the first time reports the use of cost-effectively prepared nano-engineered titanium surface via anodization, with aligned multi-scale micro/nano features for selective cellular bioactivity, without the use of any therapeutics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Single-step fabrication of dual micro-rough and nanoporous titanium surfaces </LI> <LI> Mechanically robust aligned nanopores with preserved underlying micro-features </LI> <LI> Upregulated attachment and adhesion of osteoblasts and fibroblasts </LI> <LI> Reduced macrophage proliferation and adherence </LI> <LI> Osteoblasts and fibroblasts align parallel to the nanopore arrangement. </LI> </UL> </P>
SECOND-ORDER SYMMETRIC DUALITY IN MULTIOBJECTIVE PROGRAMMING OVER CONES
GULATI, TILAK RAJ,MEHNDIRATTA, GEETA Korean Mathematical Society 2016 대한수학회지 Vol.53 No.1
In this paper, some omissions in Mishra and Lai [13], have been pointed out and their corrective measures have been discussed briefly.
Gulati, Reema,Komuravelly, Arpitha,Leb, Stephen,Mhanna, Maroun J,Ghori, Abdullah,Leon, Janeen,Needlman, Robert The Korean Society of Pediatric Gastroenterology 2018 Pediatric gastroenterology, hepatology & nutrition Vol.21 No.2
Purpose: Rome criteria are considered the gold standard for diagnosing functional constipation. The modified Bristol stool form scale (m-BSFS) was validated to measure stool form in children. However, neither the potential use of the m-BSFS as a tool to facilitate the diagnosis of potential constipation, nor the agreement between m-BSFS and stool consistency by Rome has been studied. Our objective is to determine if m-BSFS is a reliable tool to facilitate detection of constipation; and the agreement between stool form by m-BSFS and hard stool criteria in Rome. Methods: A survey tool with the Rome III criteria and the m-BSFS was developed. A Likert-scale addressed frequency of each stool form on the m-BSFS. Responses to Rome III and m-BSFS were compared. Results: The sensitivity and specificity of the m-BSFS was 79.2% and 66.0% respectively; and in children <4 years. improved to 81.2% and 75.0% respectively. There was poor agreement between hard stools by m-BSFS and the painful or hard bowel movement question of Rome Criteria. Conclusion: The potential utility of m-BSFS as a reasonably good tool to facilitate the diagnosis of potential constipation in children is shown. The poor agreement between painful or hard stool question in Rome III, and ratings for hard stool on the m-BSFS illustrates that one's perception may differ between a question and a picture. A useful pictorial tool to appraise stool form may, thus, be a favorable complement in the process of enquiry about bowel habits in well-child care.
Unreliable Automated Complete Blood Count Results: Causes, Recognition, and Resolution
Gulati Gene,Uppal Guldeep,Gong Jerald 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.5
Automated hematology analyzers generate accurate complete blood counts (CBC) results on nearly all specimens. However, every laboratory encounters, at times, some specimens that yield no or inaccurate result(s) for one or more CBC parameters even when the analyzer is functioning properly and the manufacturer’s instructions are followed to the letter. Inaccurate results, which may adversely affect patient care, are clinically unreliable and require the attention of laboratory professionals. Laboratory professionals must recognize unreliable results, determine the possible cause(s), and be acquainted with the ways to obtain reliable results on such specimens. We present a concise overview of the known causes of unreliable automated CBC results, ways to recognize them, and means commonly utilized to obtain reliable results. Some examples of unreliable automated CBC results are also illustrated. Pertinent analyzer-specific information can be found in the manufacturers’ operating manuals.
NONDIFFERENTIABLE SECOND-ORDER MINIMAX MIXED INTEGER SYMMETRIC DUALITY
Gulati, Tilak Raj,Gupta, Shiv Kumar Korean Mathematical Society 2011 대한수학회지 Vol.48 No.1
In this paper, a pair of Wolfe type nondifferentiable sec-ond order symmetric minimax mixed integer dual problems is formu-lated. Symmetric and self-duality theorems are established under $\eta_1$-bonvexity/$\eta_2$-boncavity assumptions. Several known results are obtained as special cases. Examples of such primal and dual problems are also given.
Purpose and Criteria for Blood Smear Scan, Blood Smear Examination, and Blood Smear Review
Gene Gulati,Jinming Song,Alina Dulau Florea,Jerald Gong 대한진단검사의학회 2013 Annals of Laboratory Medicine Vol.33 No.1
A microscopic examination of an appropriately prepared and well-stained blood smear by a knowledgeable laboratory professional is necessary and clinically useful in a number of circumstances and for a variety of reasons. In this article, an attempt is made to delineate the purpose and criteria for blood smear examination in a variety of circumstances that are encountered in everyday laboratory hematology practice. A blood smear scan serves to at least (a) verify the flagged automated hematology results and (b) determine if a manual differential leukocyte count needs to be performed. Blood smear examination/manual differential leukocyte count with complete blood count (CBC) provides the complete hematologic picture of the case, at least from the morphologic standpoint. Blood smear review with or without interpretation serves to ensure that no clinically significant finding is missed, besides providing diagnosis or diagnostic clue(s), particularly if and when interpreted by a physician
Jyoti Gulati,V. V. Singh,Dilip Kumar Rawal 한국신뢰성학회 2018 International Journal of Reliability and Applicati Vol.19 No.2
This paper deals with the study of reliability measures of a two-units redundant system under different types of failure and two types of repair. The system consists of two redundant units attached to the main unit. Initially, in starting, the main unit start working and perform the task. Whenever the main unit fails, one of the redundant units takes the load of the failed unit and the fail unit assigned for repair or waits for repair if the repair facility/repairman available or is not available. After, failure of any one of the redundant unit ceases the other redundant unit take a load of the failed units. Thus, the system will fail if both redundant units fail before the repair of the main unit. An operator is handling the system; hence, the human error can also appear due to mishandling the system at any stage during its operations. All failure rates constant and follows an exponential distribution, but the repair follows two types of distributions general distribution and Gumbel-Hougaard family copula distribution. The system studied by using supplementary variable and Laplace transform wherein some essential measures of reliability have discussed.