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Guimin Zhao,Hongmei Wang,Peili Hou,Chengqiang He,Hongbin He 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.2
Paratuberculosis (Johne’s disease) is a chronic debilitating disease of domestic and wild ruminants. However, widespread point-of-care testingis infrequent due to the lack of a robust method. The isothermal recombinase polymerase amplification (RPA) technique has applied for rapiddiagnosis. Herein, RPA combined with a lateral flow dipstick (LFD) assay was developed to estimate DNA from Mycobacterium avium subsp. paratuberculosis. First, analytical specificity and sensitivity of the RPA-nfo primer and probe sets were assessed. The assay successfullydetected M. paratuberculosis DNA in 30 min at 39oC with a detection limit of up to eight copies per reaction, which was equivalent to thatof the real-time quantitative polymerase chain reaction (qPCR) assay. The assay was specific, as it did not amplify genomes from five otherMycobacterium spp. or five pathogenic enteric bacteria. Six hundred-twelve clinical samples (320 fecal and 292 serum) were assessed byRPA-LFD, qPCR, and enzyme-linked immunosorbent assay, respectively. The RPA-LFD assay yielded 100% sensitivity, 97.63% specificity,and 98.44% concordance rate with the qPCR results. This is the first report utilizing an RPA-LFD assay to visualize and rapidly detect M. paratuberculosis. Our results show this assay should be a useful method for the diagnosis of paratuberculosis in resource-constrained settings.
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Zhe Zhang,Zhao Liang Wu,Guimin Liu 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.28 No.-
It is important to develop an environment-friendly collector for removing or recovering harmful nonsurface-active materials using foam fractionation. Dodecyl dimethyl betaine (BS12) was used as thecollector for studying the interfacial adsorption of methyl orange (MO) in the liquid phase. The maximaof the surface excess and the enrichment ratio of MO were 22.7% and 51.5%, respectively under pH of 5and the BS12 concentration of 0.40 g/L. The results indicated that BS12 was an effective andenvironmental collector and it can promote the development and application of foam fractionation forthe removal of harmful non-surface-active materials.
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