Plenary Session : Small Cell Carcinoma of The Esophagus: Analysis of 126 Cases

( Gui Qi Wang ),( Zhao Hui Xiong ),( Bao Qing Li ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: Small cell carcinoma of the esophagus (ESCC) is a rare and aggressive tumor with early wide-spread dissemination. Although the rarity of this tumor has impeded statistical evaluation. We reviewed records of 5.164 esophageal cancer patients treated in Shijiazhuang Municipal No.1 Hospital and Hebei tumor hospital from May, 1996 to Dec. 2006. Of 5.164 cases, 126 patients were diagnosed ESOC, with a relative frequency of 2.44%. This is one of the largest single institution series in the world literature. The purpose of this study was to analyze the clinical manifestations, pathological features and treatment of primary small cell carcinoma of the esophagus. Methods: The records of 126 patients with primary esophageal small cell carcinoma were reviewed to summarize the clinical characteristics and impact of therapy modality on the survival. Results: The median survival time of the 102 patients was 16.2 months; the 1-, 2-, and 3-year survival rates were 64.7%, 32.4%, and 9.8% respectively, 59 of the cases were followed up to exceed 5 years. Of the 59 cases, only 3 survival time were exceeded 5 years. Clinical stage was the most important prognostic factor. The median survival time of the patients received local treatment (surgical operation or radiotherapy) was 14.5 months and 9.1 mouths respectively, the 1-, 2-, 3-year survival rates were 45.8%, 27.3%, and 6.3% in local treatment cases respectively. The median survival time of the patients received local treatment plus chemotherapy were 18.0 months; the 1-, 2-, 3-year survival rates were 69.0%, 36.2%, and 10.3%, respectively. Conclusions: Small cell carcinoma of the esophagus should be regarded as a systemic disease, and multimodality treatment, including chemotherapy, should be used. Surgery may be offered in selected patients to manage local disease as part a chemotherapy-based treatment program. ESCC is a very aggressive tumor with poor prognosis.

Molecular characterization of chemosensory protein genes in Bactrocera minax (Diptera: Tephritidae)

Wang Zhao‐Xiang,Qi Zhen‐hua,Chen Jian,Wang Fu‐Lian,Gui Lian‐You,Zhang Guo‐Hui 한국곤충학회 2021 Entomological Research Vol.51 No.7

Chemoreception is of great importance for survival of insects. Chemosensory proteins (CSPs) are believed to be involved in the perireceptor events of chemosensory system in many insect species, but this has not been clarified in the citrus fruit fly, Bactrocera minax, a serious dipteran pest of citrus crops. Previous studies indicated that four CSP genes were identified in many fly speices in Diptera. In the present study, we also identified four CSP genes in B. minax,namely BminCSP1–4, from a transcriptome database. All CSP proteins encoded by these genes bear the typical hallmarks of the CSP family: an N-terminal signal peptide and the four highly conserved cysteine residues. Phylogenetic analysis comparing with other dipteran CSPs indicated that dipteran CSPs may evolved from three ancestral CSP genes, and revealed the sequence diversities of BminCSPs and showed that BminCSP1–4 are clustered in separate groups, indicating the possibility of their contrasting function in B. minax. Furthermore, the tissue distribution of the four CSP genes in adult B. minax was analyzed by real-time quantitiative PCR. The results demonstrated that BminCSP3 are significantly transcriptionally enriched in antennae; BminCSP4 expressed primarily in heads; BminCSP1 and BminCSP2 showed high expressions in different tissues, such as antennae, abdomens and wings. Based on these findings, the different implications for the functions of BminCSPs are discussed. This study will offer a significant indication for further functional studies of the CSPs in B. minax

Influence of Mist Intervals and Aeration Rate on Growth and Second Metabolite Production of Pseudostellaria heterophylla Adventitious Roots in a Siphon-mist Bioreactor

Gui Rong Wang,Nian Min Qi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.6

Plant adventitious root culture in bioreactors is a promising alternative for the efficient production of medicinal herbs. Adventitious roots of Pseudostellaria heterophylla were induced from callus and then cultivated in a siphon-mist bioreactor. An orthogonal test established that the optimal medium for adventitious root induction was MS medium supplemented with 1.0 mg/L naphthaleneacetic acid and 2.0 mg/L 3-indolybutyric acid. Under these conditions, the average root number was more than 14 on each 1.0 cm diameter callus and the rooting rate reached 100%. The bioreactor was equipped with an integral siphon-spraying device designed to automatically supply the liquid medium. The operation parameters of the bioreactor were assessed by varying the mist interval and the aeration velocity. The mist interval was negatively related to average growth rate of the adventitious roots and positively related to saponin and polysaccharide content. A relatively high aeration rate was necessary to achieve the maximum biomass production, but the secondary metabolite production was not enhanced by increasing the aeration velocity.

Cytosolic prion protein induces apoptosis in human neuronal cell SH-SY5Y via mitochondrial disruption pathway

( Xin Wang ),( Chen Fang Dong ),( Qi Shi ),( Song Shi ),( Gui Rong Wang ),( Yan Jun Lei ),( Kun Xu ),( Run An ),( Jian Ming Chen ),( Hui Ying Jiang ),( Chan Tian ),( Chen Gao ),( Yu Jun Zhao ),( Jun H 생화학분자생물학회 2009 BMB Reports Vol.42 No.7

The Research Progress of the Interactions between miRNA and Wnt/beta-catenin Signaling Pathway in Breast Cancer of Human and Mice

Ye, Ni,Wang, Bin,Quan, Zi-Fang,Pan, Hai-Bo,Zhang, Man-Li,Yan, Qi-Gui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3

MicroRNA expression is a research focus in studies of tumors. This article concentrates attention on potential links between tumors caused by mouse mammary tumor virus (MMTV) and human breast cancer, in order to provide theoretical basis for using mouse model to search for miRNA effects mediated by Wnt/beta-catenin signaling in human breast cancer. By analyzing interactions between miRNAs and the Wnt/beta-catenin signaling pathway in breast cancer, we hope to casts light on more biological functions of miRNAs in the process of tumor formation and growth and to explore their potential value in cancer diagnosis, prognosis and treatment. Our endeavor aimed at providing theoretical basis for finding safer, more effective methods for treatment of human breast cancer at the miRNA molecular level.

Genetic Variations in the HIF1A Gene Modulate Response to Adjuvant Chemotherapy after Surgery in Patients with Colorectal Cancer

Zhang, Yi,Wang, Peng,Zhou, Xing-Chun,Bao, Guo-Qiang,Lyu, Zhuo-Ming,Liu, Xiao-Nan,Wan, Shao-Gui,He, Xian-Li,Huang, Qi-Chao Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Background: Hypoxia-inducible factor $1{\alpha}$ (HIF-$1{\alpha}$) plays an important role in regulating cell survival and angiogenesis, which are critical for tumor growth and metastasis. Genetic variations of HIF1A have been shown to influence the susceptibility to many kinds of human tumors. Increased expression of HIF-$1{\alpha}$ has also been demonstrated to be involved in tumor progression. However, the prognostic value of single nucleotide polymorphisms (SNPs) inthe HIF1A gene remains to be determined in most cancer types, including colorectal cancer (CRC). In this study, we sought to investigate the predictive role of HIF1A SNPs in prognosis of CRC patients and efficacy of chemotherapy. Materials and Methods: We genotyped two functional SNPs in HIF1A gene using the Sequenom iPLEX genotyping system and then assessed their associations with clinicopathological parameters and clinical outcomes of 697 CRC patients receiving radical surgery using Cox logistic regression model and Kaplan Meier curves. Results: Generally, no significant association was found between these 2 SNPs and clinical outcomes of CRC. In stratified analysis of subgroup without adjuvant chemotherapy, patients carrying CT/TT genotypes of rs2057482 exhibited a borderline significant association with better overall survival when compared with those carrying CC genotype [Hazard ratio (HR), 0.47; 95% confidence interval (95% CI): 0.29-0.76; P < 0.01]. Moreover, significant protective effects on CRC outcomes conferred by adjuvant chemotherapy were exclusively observed in patients carrying CC genotype of rs2057482 and in those carrying AC/CC genotype of rs2301113. Conclusions: Genetic variations in HIF1A gene may modulate the efficacy of adjuvant chemotherapy after surgery in CRC patients.

CADMIUM TELLURIDE NANOCRYSTALS: SYNTHESIS, GROWTH MODE AND EFFECT OF REACTION TEMPERATURE ON CRYSTAL STRUCTURES

XIAO-YING QI,KAN-YI PU,QU-LI FAN,DUO-FENG TANG,GUI-AN WEN,FREDDY Y. C. BOEY,LIAN-HUI WANG,WEI HUANG,HUA ZHANG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2008 NANO Vol.3 No.2

A series of cadmium telluride (CdTe) nanocrystals were synthesized by a modified organometallic synthesis method at various reaction temperatures ranging from 130 to 250°C. In this method, octadecylamine (ODA) was introduced as an additional coordinating component to the mixture of trioctylphosphine oxide (TOPO) and trioctylphosphine (TOP). CdO was used as a precursor. The prepared CdTe nanocrystals were studied by the absorption and emission spectra as well as the powder X-ray diffraction (XRD) patterns. The result shows that besides the traditional continuous-growth mode observed frequently at relatively high reaction temperature, a discontinuous-growth mode was confirmed at the initial growth stage of CdTe nanocrystals, arising from the change of the absorption spectra of CdTe nanocrystals with the reaction time at relatively low reaction temperature. The structures of CdTe nanocrystals, e.g., the cubic zinc blende structure at 160°C and the hexagonalwurtzite structure at 250°C, were characterized by XRD.

Functional Roles of Long Non-coding RNA in Human Breast Cancer

Ye, Ni,Wang, Bin,Quan, Zi-Fang,Cao, San-Jie,Wen, Xin-Tian,Huang, Yong,Huang, Xiao-Bo,Wu, Rui,Ma, Xiao-Ping,Yan, Qi-Gui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

The discovery of long noncoding RNA (LncRNA) changes our view of transcriptional and posttranscriptional regulation of gene expression. With application of new research techniques such as high-throughput sequencing, the biological functions of LncRNAs are gradually becoming to be understood. Multiple studies have shown that LncRNAs serve as carcinogenic factors or tumor suppressors in breast cancer with abnormal expression, prompts the question of whether they have potential value in predicting the stages and survival rate of breast cancer patients, and also as therapeutic targets. Focusing on the latest research data, this review mainly summarizes the tumorigenic mechanisms of certain LncRNAs in breast cancer, in order to provide a theoretical basis for finding safer, more effective treatment of breast cancer at the LncRNA molecular level.

Disorders of Liver : The Study Of The Relationship Between Morphological Characteristics Of Tumor Microvessel And The Clinical Pathology Features Of The Hepatocellular Carcinoma

( Zhuan Yi Cui ),( Gui Qi Wang ) 대한소화기학회 2007 SIDDS Vol.9 No.-

Background/Aims: There is sufficient evidence that tumour microvessels are pathology foundation and requisite condition of infarctate tumor in growthing and transferring. It is more to study primary hepatocellular carcinoma microvessels densities, while it is few to study the relation between microvessels morphous and tumor`s clinical pathology features. This aim of study is to explore the relation between the morphological characteristics of tumor microvessels and the clinical pathology features of hetatocellular carcinoma (HCC). Methods: To collect 55 primary hepatocellular carcinoma tissues preoperatively without artery embolotherapy, and there microvessels appearance was divided into 4 types: punctiform, filate, cireunduction, strip. The microvessels typing, differentiation level, tumors diameter, envelope and satellitic nubble were surveyed. Methods: To collect 55 primary hepatocellular carcinoma tissues preoperatively without artery embolotherapy, which was divided into 4types by microvessels modality: punctiform, filate, circumduction, strip. The microvessels typing, differentiation level, tumors diameter, envelope and satellitic nubble were surveyed. Results: Tumor microvessels typing degrees has the tendency of positive correlation with differentiation level and tumors diameter. Tumor microvessels typing degrees in HCC without envelope is higher than that in HCC with envelope. Tumor microvessels typing is no difference between HCC without satellitic nubble and HCC with satellitic nubble. Conclusions: The morphological Characteristics of HCC`s tumor microvessel were distinctive in different vegetal phases, and had definite rule. Tumor microvessels typing degrees is higher in low-grade differentiation level HCC, bigger HCC and HCC without envelope.

Clinical Outcomes of Basaloid Squamous Cell Carcinoma of the Esophagus: A Retrospective Analysis of 142 Cases

Zhang, Bai-Hua,Cheng, Gui-Yu,Xue, Qi,Gao, Shu-Geng,Sun, Ke-Lin,Wang, Yong-Gang,Mu, Ju-Wei,He, Jie Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Background: Basaloid squamous cell carcinoma of the esophagus (BSCCE) is a rare and distinctive tumor with no standard treatment. This study aimed to explore treatment in relation to prognosis of the disease. Methods: A total of 142 patients with BSCCE that underwent treatment in our hospital from March 1999 to July 2010 were retrospectively analyzed. All patients received surgery, 42 postoperative radiotherapy and 28 patients chemotherapy. Results: There were 26 patients included in stage I, 60 in stage II, 53 in stage III and 3 in stage IV. The clinical symptoms and macroscopic performances of BSCCE did not differ from those of typical esophageal squamous cell carcinoma. Among 118 patients receiving endoscopic biopsy, only 12 were diagnosed with BSCCE. The median survival time (MST) of the entire group was 32 months, with 1-, 3- and 5-year overall survival (OS) of 81.4%, 46.8% and 31.0%, respectively. The 5-year OS of stage I and II patients was significantly longer than that of stages III/IV, at 60.3%, 36.1% and 10.9%, respectively (p<0.001, p=0.001). The MST and 5-year OS were 59.0 months and 47.4% in patients with tumors located in the lower thoracic esophagus, and 27.0 months and 18.1% in those with lesions in the upper/middle esophagus (p=0.002). However, the survival was not significantly improved in patients undegoing adjunctive therapy. Multivariate analysis showed TNM stage and tumor location to be independent prognostic factors. Furthermore, distant metastasis was the most frequent failure pattern, with a median recurrence time of 10 months. Conclusion: BSCCE is an aggressive disease with rapid progression and a propensity for distant metastasis. It is difficult to make a definitive diagnosis via preoperative biopsy. Multidisciplinary therapy including radical esophagectomy with extended lymphadenectomy should be recommended, while the effectiveness of radiochemotherapy requires further validation for BSCCE.