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Genetic Analysis and Molecular Mapping of Dull Gene in Low Amylose Rice Cultivar, Milyang262
Gilang Kiswara,Jong-Hee Lee,Yeon-Jae Hur,Jun-Hyun Cho,Ji-Yoon Lee,Sang-Yeol Kim,Yeong-Bo Sohn,Choon-Woo Lee,You-Chun Song,Kyung-Min Kim,Min-Hee Nam 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07
Amylose content of rice endosperm is one of the determinants of rice eating quality. This study was conducted to elucidate the mode of inheritance of dull gene in Milyang262, tentatively designated as du7(t), and to identify the molecular marker for du7(t) to be employed in marker-assisted breeding and gene pyramiding. Genetic analysis was carried out on F2 population derived from a cross between Junam and Milyang262. The low amylose content of Milyang262 was indicated to be under single recessive control. Allelism tests were as well conducted by crossing Milyang262 with Baegjinju and Baegokchal, which harbor du1 and wx gene, respectively. du7(t) was demonstrated to be inherited independently to du1 and wx. F2 population of Baegokchal/Milyang262 was used for molecular mapping. Linkage analysis was conducted on a population consisted of 120 individuals by several SSR markers. Initial mapping indicated that du7(t) is located on the end of long arm of chromosome 6 between SSR marker RM20590 and RM3509. To fine map the gene, a bigger population and several additional markers were employed. du7(t) was further mapped to a 1.74 Mb region between two SSR markers (RM6926 and RM412). Furthermore, we indentified three SSR markers that co-segregated with du(t) i.e. RM6811, RM3765, and RM176.
Natural and anthropogenic impacts on mangrove carbon dynamics: a systematic review protocol
Gilang Qur’ani Citra,이보라,Sasmito Sigit D.,Maulana Agus Muhamad,설미현,Wiradana Putu Angga,Leksono Budi,Watiniasih Ni Luh,Baral Himlal 한국산림과학회 2024 Forest Science And Technology Vol.20 No.1
The mangrove ecosystem serves as a vital habitat for coastal flora and fauna while playing a crucial role in storing and sequestering carbon as part of global carbon cycles. Therefore, it is imperative to evaluate the carbon dynamics, encompassing storage and sequestration, within mangrove ecosystems and their interconnectedness with natural climate fluctuations and anthropogenic influences, including land-use and land-cover changes (LULCC). Although there has been an increase in monitoring data and literature on mangrove carbon dynamics over the past two decades, there is still limited understanding regarding how climate variability, when combined with anthropogenic drivers, moderates the resilience of carbon storage and sequestration in mangroves. This study aims to build upon and enhance the previous systematic review conducted by Sasmito et al. (2019). Our specific objectives involve collating more recent literature published since 2018 and strengthening the analysis of carbon loss and recovery in tree biomass across different species, as well as its correlation with local and regional climate variations. Additionally, we will explore the impact of various types of land-use and land-cover changes on mangrove forests. Our systematic review will focus on field-based data collected from the Asia Pacific mangrove region, which represents the world’s largest and most diverse mangrove ecosystem and has been extensively studied in comparison to other regions, as indicated by previous systematic reviews. To gather relevant literature, we will conduct comprehensive searches across various databases, including Scopus, Web of Science, and Google Scholar. The structure established by Sasmito et al. (2019) for literature search, screening, and data extraction will be adopted. Data analysis will involve comparing carbon storage and sequestration under locally and regionally varying climatic conditions and anthropogenic influences. Furthermore, we will employ geographical mapping techniques to visualize species distribution and diversity with
Characteristics of Magnetic Sengon Wood Impregnated with Nano Fe3O4 and Furfuryl Alcohol
Gilang Dwi LAKSONO,Istie Sekartining RAHAYU,Lina KARLINASARI,Wayan Darmawan,Esti PRIHATINI 한국목재공학회 2023 목재공학 Vol.51 No.1
Sengon (Falcataria moluccana Miq.) tree offers a wood of low quality and durability owing to its low density and thin cell walls. This study aimed to improve the properties of sengon wood by making the wood magnetic, producing new functions, and characterizing magnetic sengon wood. Each wood sample was treated using one of the following impregnation solutions: Untreated, 7.5% nano magnetite-furfuryl alcohol (Fe3O4-FA), 10% nano Fe3O4-FA, and 12.5% nano Fe3O4-FA. The impregnation process began with vacuum treatment at 0.5 bar for 2 h, followed by applying a pressure of 1 bar for 2 h. The samples were then tested for dimensional stability and density and characterized using scanning electron microscopy and energy-dispersive X-ray spectroscopy (SEM–EDX), Fourier transform infrared spectrometry (FTIR), X-ray diffraction (XRD) analysis, and vibrating sample magnetometry (VSM) analysis. The results showed that the Fe3O4-FA impregnation treatment considerable affected the dimensional stability, measured in terms of weight percent gain, anti-swelling efficiency, water uptake, and bulking effect, as well as the density of sengon wood. Changes in wood morphology were detected by the presence of Fe deposits in the cell walls and cell cavities of the wood using SEM–EDX analysis. XRD and FTIR analyses showed the appearance of magnetite peaks in the diffractogram and Fe-O functional groups. Based on the VSM analysis, treated sengon wood is classified as a superparamagnetic material with soft magnetic properties. Overall, 10% Fe3O4-FA treatment led to the highest increase in dimensional stability and density of sengon wood.
Gilang Gumilar,Ni Luh Wulan Septiani,Shofarul Wustoni,Taufik Ramdani Tohari,Wahyu Widayat,Muhammad Yusuf,Hesti L. Wiraswati,Lia Faridah,Savira Ekawardhani,Isa Anshori,Brian Yuliarto 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.127 No.-
The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus has become a dangeroushuman pathogen and caused severe illness that can lead to death. Yet, the standard practical diagnostictools for detecting the SARS-CoV-2 virus require multi-step assay, including extraction and labeling steps. Thus, a simple and accurate detection tool is incredibly desirable to support on-site screening, preventingits spread. In this work, we developed a portable surface plasmon resonance (SPR) sensor chip modifiedwith metal–organic frameworks (MOF) UiO-66 that serves as an immobilization matrix of single-chainvariable fragment (scFv) receptors. Large surface area and good adsorption of UiO-66/scFv-modified surfacelead to an improvement of binding capacity towards the protein target of the SARS-CoV-2 receptorbinding domain (RBD). We further successfully demonstrated the use of this portable SPR to detect theSARS-CoV-2 virus from patients’ nasopharyngeal swab samples. Interestingly, the SPR sensors candirectly distinguish the positive and negative patients within 15 minutes on 23 samples, simultaneously,without the need for labeling, and the results are in line with the gold standard PCR tests. In addition, thedetection limit of the sensors is 10.085 105 virus/mL, which follows the standard of practical screeningtools. Therefore, these UiO-66 modified SPR chip sensors offer a rapid, label-free, and low-cost detectionof the SARS-CoV-2 virus that can be potentially developed for other infectious diseases.