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( Gang Zhou ),( Long Jie Li ),( Qing Shan Shi ),( You Sheng Ouyang ),( Yi Ben Chen ),( Wen Feng Hu ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.12
Citrobacter sp. is a cause of significant opportunistic nosocomial infection and is frequently found in human and animal feces, soil, and sewage water, and even in industrial waste or putrefaction. Biofilm formation is an important virulence trait of Citrobacter sp. pathogens but the process and characteristics of this formation are unclear. Therefore, we employed in vitro assays to study the nutritional and environmental parameters that might influence biofilm formation of C. werkmanii BF-6 using 96-well microtiter plates. In addition, we detected the relative transcript levels of biofilm formation genes by RT-PCR. Our results indicated that the capacity of C. werkmanii BF-6 to form biofilms was affected by culture temperature, media, time, pH, and the osmotic agents glucose, sucrose, NaCl, and KCl. Confocal laser scanning microscopy results illustrated that the structure of biofilms and extracellular polysaccharide was influenced by 100 mM NaCl or 100 mM KCl. In addition, nine biofilm formation genes (bsmA, bssR, bssS, csgD, csgE, csgF, mrkA, mrkB, and mrkE) were found to contribute to planktonic and biofilm growth. Our data suggest that biofilm formation by C. werkmanii BF-6 is affected by nutritional and environmental factors, which could pave the way to the prevention and elimination of biofilm formation using proper strategies.
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( Ouyang Ping ),( Sun Mao ),( He Xuewen ),( Wang Kaiyu ),( Yin Zhongqiong ),( Fu Hualin ),( Li Yinglun ),( Geng Yi ),( Shu Gang ),( He Changliang ),( Liang Xiaoxia ),( Lai Weiming ),( Li Lixia ),( Zou 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.1
Staphylococcus aureus (S. aureus) is a common gram-positive bacterium that causes serious infections in humans and animals. With the continuous emergence of methicillin-resistant S. aureus (MRSA) strains, antibiotics have limited efficacy in treating MRSA infections. Accordingly, novel agents that act on new targets are desperately needed to combat these infections. S. aureus alpha-hemolysin plays an indispensable role in its pathogenicity. In this study, we demonstrate that sclareol, a fragrant chemical compound found in clary sage, can prominently decrease alpha-hemolysin secretion in S. aureus strain USA300 at sub-inhibitory concentrations. Hemolysis assays, western-blotting, and RT-PCR were used to detect the production of alpha-hemolysin in the culture supernatant. When USA300 was co-cultured with A549 epithelial cells, sclareol could protect the A549 cells at a final concentration of 8 μg/ml. The protective capability of sclareol against the USA300-mediated injury of A549 cells was further shown by cytotoxicity assays and live/dead analysis. In conclusion, sclareol was shown to inhibit the production of S. aureus alpha-hemolysin. Sclareol has potential for development as a new agent to treat S. aureus infections.
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Gang Wang,Baisha Ouyang,Fang Jing,Xiaoyan Dai 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.1
The poor outcome of advanced ovarian cancer under conventional therapy necessitates new strategies to improve therapeutic efficacy. β-glucosidase (encoded by GBA) is a lysosomal enzyme and is involved in sphingolipids metabolism. Recent studies revealed that β-glucosidase plays a role in cancer development and chemoresistance. In this work, we systematically evaluated the expression and role of GBA in ovarian cancer. Our work demonstrates that inhibition of β-glucosidase has therapeutic potential for ovarian cancer. Gene Expression Profiling Interactive Analysis database, western blot and immunohistochemistry analyses of patient samples demonstrated that GBA mRNA and protein expression levels were significantly increased in ovarian cancer compared to normal tissues. Functional studies using gainof- function and loss-of-function approaches demonstrated that GBA overexpression did not affect growth and migration but alleviated cisplatin’s efficacy in ovarian cancer cells. In addition, GBA depletion resulted in growth inhibition, apoptosis induction, and enhancement of cisplatin’s efficacy. Of note, we found that GBA inhibition specifically decreased receptor tyrosine kinase AXL level, leading to the suppression of AXL-mediated signaling pathways. Our data suggest that GBA represents a promising target to inhibit AXL signaling and overcome cisplatin resistance in ovarian cancer.
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Sanwen Peng,Gang Ouyang,Xiangwei Cao,Ning Hou,Guangyuan Yang,Fei Yan,Fengxiang Xu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.6
In this work, we describe experimental water vapor forming of hollow filter rods. The morphology, microstructure and mechanical property of hollow filter rods were carefully investigated. The forming mechanism of hollow filter rods was also discussed. The results demonstrated that a well-formed product was successfully obtained under the best process parameters. The circumference means of tested samples can be mainly determined by water vapor pressure and plasticizer concentration. However, the roundness of hollow filter rods depended on cellulose tow content. The occurrence of surface fuzzing and bending deflection was largely as the result of incomplete curing effect while the emergence of intermittent formation may rely on the tow feed volume during the forming process. The acceptable hardness of hollow filter rods was up to 92.27 % in the experiments. An improvement in hardness was mainly attributive to crisscrossing three-dimensional structures formed inside the filter rod. A model consisting of the diffusion stage, crosslinking stage and solidifying stage was proposed to illustrate the formation of filter rods.
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