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Gamze ER,Alexander JAHN,Shah Abid ALI,Man-Gi CHO 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4
Biofilm-based Photobioreactors (PBRs) use microalgal biomass immobilized on a substrate with reduced water content and consumption when compared to conventional suspended cultivation. CO2 and carbonate are used by microalgae as inorganic carbon source and their consumption is an indicator for metabolic activity and biomass accumulation. Here CO2 uptake, holdup and release in and from the culture were measured and correlated with gravimetrically determined biofilm mass. For this, a wicking based flat panel biofilm PBR including CO2/O2 gas control was developed, and the CO2 uptake and holdup determined by CO2 NIR off-gas analysis. The cyanobacteria Chlamydomonas asymmetrica were cultivated on hydrophilic modified expanded UHMWPE plates over circulating AF6 medium at 30°C and fluorescent light illumination about 710.01 µW/cm<sup>2</sup>. CO2 holdup in the system was subtracted from values determined during biofilm cultivation at stationary and growth conditions and correlated with measured biomass. From the gas holdup a minimum feasible cultivation size, dependent on the NIR-CO2 sensor sensitivity, for the use of the developed method was estimated. Results suggest the potential use of gas holdup measurement for biofilm growth assessment in medium and large-scale closed cultivations.