물리ㆍ화학적 방법을 이용한 위생적인 해상빙의 제조방법 개발

조덕제,조만기,이정석 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

In order to make hygienic fishery ice, sea and city water were pretreated by physical method such as ultrafitration, ultraviolet sterilizer and heating. Among of the three treatments, U,V-light is the best method for application in ice-making enterprise practically. The fishery ice containing 3% salt decreased the contamination of microoganism and improved the ability of low temperature preservation. Key words: hygienic fishery ice, physical and chemical method.

Dimensional Analysis and Scale-up in Biotechnology

조덕제,조만기 東西大學校 1996 동서논문집 Vol.2 No.1

The biochemical engineer is generally concerned with the industrial implementation of proceesses in which chemical or microbiological conversion of material take place in conjunction with the transfer of mass, heat, and momentum. These processes are scale-dependent, i.e., the fermentation, immobilization and contaminated soil treatment etc.. Understandably, biochemical engineer have always wanted to gain insight that will assist them in designing new industrial plants. Occasionally, they are faced with the same problem for another reason: an industrial facility already exists but will not function properly, if at all, and suitable measurements have to be carried out to discover the cause of the difficulties and provide a solution.

Microcapsule-membrane에서의 물질 이동 및 확산 특성

조만기 동서대학교 부설 연구소 1996 연구소 논문집 Vol.1 No.-

Hollowsphere에 대한 확산 계수를 구하기 위해서 solidsphere에 대한 확산 계수를 다음과 같이 수정하여 제시하였다. 첫째로 hollowsphere내에서 용질의 호가산 계수는 고분자막과 액체 film을 통한 내부 sphere 용액에서 확산 계수의 조합이고, 이들 조합은 물질이동 특성에 의존한다. 둘째로 물질이동 이론으로 물질이동 계수들간의 연관성은 확산 계수들 사이에서 하나의 개념 혹은 값으로 단순화 될 수 있다. 고분자막에서 용질의 확산 계수는 시간경과에 따른 용질의 양과 hollowsphere 크기와의 관계를 통한 수학적 모델로 측정할 수 있다. 물의 구종와 ??-alginate gel막의 구조는 서로 다르기 때문에 이들 간의 확산계수 값은 같을 수가 없다. 막의 두께가 두꺼워질수록 glucose의 확산율이 낮아지는 사실은 물에서의 확산 계수보다 막에서의 확산 계수가 더 낮다는 사실을 입증한다. sodium cellulose sulfate-polydiallydimethyl-ammonium chloride(NaCS-PDADMAC) 막과 ??-alginate gel 막에서 glucose의 확산 계수 data는 hollowsphere에 대한 물질이동 연구를 위한 중요한 기초 자료를 제공한다. NaCS-PDADMAC막에서 glucose의 확산 계수는 물에서의 호가산 계수보다 작았으며, ??-alginate gel막도 물에서의 호가산 계수보다 약간 작았으나 큰 차이가 없었고, 그것이 sphere에서 용액으로 이동하거나 용액에서 sphere로 이동하는 기질의 확산에는 거의 영향을 미치지 못하였다.

쑥을 첨가한 두부의 제조 및 저장 중 품질변화

조덕제,정숙현,조만기 東西大學校 1996 동서논문집 Vol.2 No.1

쑥의 효과적인 이용방법을 위해서 쑥의 최적건조 조건을 설정하고, 대두와 숙의 기능적 특성을 잘 살린 쑥을 첨가한 두부의 최적 가공조건과 제품의 저장 중 품질변화에 대해서 실험한 결과를 요약하면 다음과 같다. 1. 건조과정 중 쑥은 조단백질, 조지방 및 조회분은 감소하였으며, 반면 탄수화물은 증가하였다. 2. 1% NaHCO3에 데치기 하여 동결건조한 제품(FDB)이 쑥의 색도유지가 가장 잘 되었으며, Chlorophyll 잔존률도 가장 많았다. 3. 쑥을 첨가한 두부의 제조에서 응고제 GDL, 응고농도 0.1%, 응고온도 70-80℃, 쑥분말 첨가량 0.2%가 최적가공 조건이었다. 4. 쑥을 첨가한 두부의 저장에서 색도변화 및 TBA가의 변화로 미루어 보아 30℃에서는 12시간, 5℃에서는 4일 정도까지 저장 가능한 것으로 판단되었다. An investigation was carried out to study the optimum drying condition of mugwort, the optimum processing conditions of soybean curd added mugwort, and the quality change during storge of soybean curd added mugwort. Crude protein and crude lipid contents of mugwort were decreased during drying process, the contents of those in dried mugwort were 18.0-21.2%, 1.3%-4.0%, respectively. The freeze drying after blanching in 1% NaHCO3 solution(FDB) was the best in color index of dried mugwort, also the highest in retention ratio of total chlorophyll content. The yield of soybean curd with coagulants were showed that MgCl2, CaCl2, CaSO4 and GDL were 3.4g, 3.0g, 3.5g and 4.2g per 30g soy milk, respectively. GDL showed the highest yield among coagulants, and amount of GDL was suited at 0.1%. The optimum coagulation temperature was 70-80℃, addition amount of powered mugwort was 0.2%. The change of color index and TBA value were increased during storage. The quality of soybean curd added mugwort was maintained up to 12 hrs or 4 days at 30℃ or 5℃ storage, respectively.

Chlorella vulgaris의 배양을 위한 Photobioreactor에 관한 연구

조만기,이정석,조덕제 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

Until now, microalgae(Chlorella vulgaris), commonly known as seaweeds, are a rich and diverse source of unique feed for rotifer and microalgae and were cultivated mainly in an conventional open raceway pond photobioreactor. In this work, new photobioreactors were developd to avoid disadvantages of conventional photobioreactor. Higher growth rates could be achieved. As the light is the key factor for culturing microalgae, we focused on a sufficient supplement of kight for the microalgae. The developed photobioreactors were cylinder photobioreactor, spherical surface photobioreactor-Ⅰ, spherical surface photobioreactor-Ⅱ, plate type photobioreactor, raceway pond type photobioreactor and water wheel type photobioreactor. It was shown that the spherical surface photobioreactor-Ⅱ was the best one in respect to ??[1.05day??], also more resistant against drying up and pollution.

Bacillus sp. LJ-25가 생성하는 extracellular chitinase의 정제 및 특성

주동식,조덕제,이정석,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

The extracellular chitinase from Bacillus sp. LJ-25 was pruified Sephadex G-100 gel filtration, DEAE-Cellulose and CM-Spephadex C25 column chromatography. The pruified chitinase showed a single band on disc and SDS polyacrylamide gel electrophoresis and the molecular weight was estimated to be about 50KDa. The Optimum pH and temperature for the activity of the purified extracellular chitinase were 7.5 and 35℃, respectively. The activity of chitinase was strongly inhibited by metal ions such as Zn²+, Ba²+, Co²+, Mn²+ and Cu²+. The purified chitinase did not hydrolyze N,N??-diacetylchitobiose, N,N, N'-triacetylchitotriose and p-nitrophenol-N-acetyl-β-D-glucosaminide, which are known to be the substrates of exo-type chitinase. The products in hydolysis of colloidal chitin with the chitinase were N-acetylglucosamine, N,N'-diacetylchitobiose and N,N,N'-triacetylchitotriose.

컴퓨터 시뮬레이션을 통한 치어사육용 다단계 배양시스템의 개발 가능성 탐색

정은수,조덕제,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

It was researched that the possibility for developing multistage culture system to cultivate larvae by computer simulation with basic experiments. This culture system was composed of 3 stages. Chlorella sp. at the 1st stage are being supplied to the rotifer(Brachionus plicatilis) at 2nd stage, and the rotifer are bing feeded to larvae at the 3rd stage. In this study, Chlorella sp. were cultivated by batch culture to search for the possibility of continuous feeding rate Chlorella sp., ???????? and ??(cells/hㆍrotifer) in the multistage culture system, then the change of the rotifer concentration at 2nd stage was simulated by computer. The required amount of rotifer for the growth of larvae was also increased as the increase of the length of larvae. On the 9th day of the culture, the rotifer uptake rate of larvae was 250(cells/dayㆍlarvae). Based on these basic experiments and results. It was suggested that the possibility of multistage culture system to cultivate larvae with continuous feeding of Chlorella sp. and rotifer.

후천성 이중 유문 1예

정규성,조기섭,정재용,조영호,민영돈,박찬국,김만우 조선대학교 1994 The Medical Journal of Chosun University Vol.19 No.1

Double pyrolus, one of the relatively rare anomalies of the gastrointestinal tract, is a fistulous communication between the gastric antrum and the duodenal bulb. The first case of a double Pylorus was reported in 1969 by Smith, since then several cases have been reported. Recently, as the frequency of examining upper gastrointestinal series and endoscopy increases, it occurs more frequently and a few cases have been described in our country. According to the reports previously published, it resulted only from cysts, diverticla, atresia, band, membrane malformation of the pylorus, however, it might be also caused by penetration, complication of peptic ulcer. We report a case of double pylorus in a 67-year old man with prolonged administration of corticostetoids due to lumbago who has complained epigastric pain which was confirmed by endoscopy and biopsy.

키틴 분해균 Bacillus sp. LJ-25의 분리 및 효소생산 최적화

이정석,주동식,조만기,이응호 동서대학교부설연구소 1997 연구소 논문집 Vol.2 No.-

In order to produce functional chitin oligosaccharides, a chitinolytic becterium was newly screened from the viscera of Korean bony fish, and identified as Bacillus sp. LJ-25. For the production of chitinolytic enzyme, 1.0% nutrient broth and 0.3% colloical chitin was used as nitrogen and carbon source, respectively. The optimal temperature, initial pH and conentration of NaCl for the production of chitinase from Bacillus sp. LJ-25 were 30℃, 6.5-7.0 and 1.0%, rspectively. Chitinolytic activity of Bacillus sp. LJ-25 was increased until the incubation of 168 hours, but after 168 hours, the activity was decreased.

빛의 파장에 따른 광합성 미세조류의 성장

김현주,이정석,조만기 동서대학교부설연구소 1998 연구소 논문집 Vol.3 No.-

The growth of photoautotrophic organism such as microalgae occurs through photosynehesis in which light is the basic limiting factor in terms of photobiology and photobiotechnology. The light harvesting pigments found in microalgae are chlorphyll, phycobiliprotein and carotenoid. The concentration of photosynthetic pigments and growth are effected by the environment-specifically, photonflux density. Therefore, adaptations of microalgae to photon flux density were studied and compared in Chlorella vulgaris and Spriulina platensis. Chlorella vulgaris and Spirulina platensis were cultured under illumination of red, green, blue, and white spectrum light with equivalent photon flux density(77 μ Em??s?? between 400 and 700nm). Cultures of Cholorella vularis grown under red, green, blue and white light expressed similar maximum specific growth rates (1.3842 day??, 1.3250 day??, 1.4226, 1.4609 day??, respectively). However, maximum specific growth rates of spirlina platensis in red(0.8142 day??) and white (0.7493 day??) light-grown culture were higher than those in green (0.4404 day??) and blue light (0.4485 day??). Since phycobiliproteins aborbed and orange light were associated with photosystemⅡ and transfered absorbing light energy predominatly to photosytem Ⅱ, red and white light including light-absorbing spectrum of phycobiliprotein were effective for growth of Spirulina platensis.