http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Victor E. Balderas Hernandez,Luz M. T. Paz Maldonado,Emilio Medina Rivero,Ana P. Barba de la Rosa,Leandro G. Ordonez Acevedo,Antonio De Leon Rodriguez 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.1
The production of human interferon gamma (hIFN-γ) using a synthetic gene in Escherichia coli BL21-SI was optimized by response surface methodology (RSM) and a Box-Behnken design. The process variables studied were temperature, biomass concentration at induction time and the NaCl concentration as inducer. According to the Box-Behnken design, a second order response function was developed. The optimal expression conditions were a temperature of 32.6℃, induction biomass of 0.31 g/L and 0.3 M NaCl in minimal medium. The model prediction for the maximum hIFN-γ production was 77.3 mg/L, which corresponded satisfactorily with the experimental data. The hIFN-γ concentration attained under optimized conditions was 13-times higher than that obtained using the non-optimized conditions. We conclude that RSM is an effective method for the optimization of recombinant protein expression using synthetic genes in E. coli.
Miguel Fdo. Salazar,María del Rocío Estrada Hernández,Erick Gómez Apo,Laura G. Chávez Macías,Carlos Alfonso Rodríguez Álvarez 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.5
We report a unique case of a 4-year-old girl with an intriguing fibrohistiocytic tumour. Magnetic resonance imaging scans showed a dural mass of variegated intensity compressing the left occipital pole and apparently extending toward the superior sagittal sinus. Grossly, the cut surface of the surgical specimen was yellow, pale, and soft with reddish kernel-like crusts. Histologically, the yellow areas resembled cholesterol granulomas with widespread coagulative necrosis, cholesterol clefts, powdery calcification, foreign body-type giant cells, and foamy macrophages, while the scattered red spots contained numerous multinucleated giant cells of foreign-body and Touton types, the former with amphophilic to slightly eosinophilic cytoplasm. Immunoperoxidase reactions confirmed the expression of histiocytic markers and vimentin. As far as we know, no tumour displaying these peculiar morphological features has yet been described.
María de los Ángeles Carrasco-Ruiz,Laura G. Hernández-Aragón,Jesús Ramsés Chávez-Ríos,Jorge Rodríguez-Antolín,Pablo Pacheco,Margarita Martínez-Gómez,Estela Cuevas-Romero,Francisco Castelán 대한배뇨장애요실금학회 2018 International Neurourology Journal Vol.22 No.3
Purpose: To characterize the relationship between serum estradiol levels and the expression of glucose transporter type 4 (Glut4) in the pubococcygeus and iliococcygeus muscles in female rats. Methods: The muscles were excised from virgin rats during the metestrus and proestrus stages of the estrous cycle, and from sham and ovariectomized rats implanted with empty or estradiol benzoate–filled capsules. The expression of estrogen receptors (ERs) was inspected in the muscles at metestrus and proestrus. Relative Glut4 expression, glycogen content, and serum glucose levels were measured. Appropriate statistical tests were done to identify significant differences (P≤0.05). Results: The pubococcygeus and iliococcygeus muscles expressed ERα and ERβ. Glut4 expression and glycogen content in the pubococcygeus muscle were higher at proestrus than at metestrus. No significant changes were observed in the iliococcygeus muscle. In ovariectomized rats, the administration of estradiol benzoate increased Glut4 expression and glycogen content in the pubococcygeus muscle alone. Conclusions: High serum estradiol levels increased Glut4 expression and glycogen content in the pubococcygeus muscle, but not in the iliococcygeus muscle.
I. Merino Rodríguez,A. Hernandez-Solís,N. Messaoudi,G. Van den Eynde 한국원자력학회 2020 Nuclear Engineering and Technology Vol.52 No.10
The Nuclear Fuel Cycle Code “ANICCA” has been developed by SCK•CEN to answer particular questions about the Belgian nuclear fleet. However, the wide range of capabilities of the code make it also useful for international or regional studies that include advanced technologies and strategies of cycle. This paper shows the main features of the code and the facilities that can be simulated. Additionally, a comparison between several codes and ANICCA has also been made to verify the performance of the code by means of a simulation proposed in the last NEA (OECD) Benchmark Study. Finally, a case study of the Belgian nuclear fuel cycle phase out has been carried out to show the possible impact of the transmutation of the minor actinides on the nuclear waste by the use of an Accelerator Driven System also known as ADS. Results show that ANICCA accomplishes its main purpose of simulating the scenarios giving similar outcomes to other codes. Regarding the case study, results show a reduction of more than 60% of minor actinides in the Belgian nuclear cycle when using an ADS, reducing significantly the radiotoxicity and decay heat of the high-level waste and facilitating its management.
Nelida A. Gonzalez Hernandez,Rebeca O. Millan Guerrero,Gabriel Ceja Espiritu,Luz M. Baltazar Rodriguez,Rafael Rodriguez Salazar,Bertha A. Olmedo Buenrostro,Irma G. Enriquez Maldonado,Valery Melnikov,J 한국유전학회 2008 Genes & Genomics Vol.30 No.6
The action of matrix metalloproteinases (MMPs) can weaken the arterial wall, contributing to the destabilizing and rupture of atheromatous plaque. Within the MMPs, type 2 stands out due to its action on basement membrane constituents. Previous studies have revealed elevated levels of MMP-2 in the acute phase of ischemic stroke (IS). An MMP-2 single nucleotide polymorphism, -1306T>C (rs243865), displayed strikingly high promoter activity with the C allele. Our study analyzed whether or not the MMP-2-1306T>C polymorphism contributed to the development of IS in a Mexican population. Ninety-eight patients with IS and 213 control subjects were analyzed. Genomic DNA isolation, polymerase chain reaction (PCR), and polymorphism detection by restriction enzyme digestion were performed to detect MMP-2-1306T>C polymorphism. An increased probability of IS associated with the MMP-2 CC genotype (OR, 1.70; 95% CI, 1.02-2.85) was found. IS risk associated with the CC genotype was more pronounced in hypertensive subjects (OR, 2.81; 95% CI, 1.4-5.5). The CC genotype was not associated with the development of primary hypertension. The data suggest that MMP-2-1306T>C polymorphism greatly contributes to IS development in the population studied, principally in hypertensive subjects. This case-control study lends support to the association of MMP-2 with stroke at the genetic level, an association consistent with MMP-2 participation in IS physiopathology.
Sintering of Mechanically Alloyed YSZ Nanocrystalline Powders
De la Torre,M. A. Lopez,Dura O. J.,Hernandez M.,Garcia-Cordobes M.,Herranz G.,Sanchez-Bautista C.,Rodriguez G. P. 한국분말야금학회 2006 한국분말야금학회 학술대회논문집 Vol.2006 No.1
We report on the mechanical and structural properties of nanocrystalline 8% and 10% mol yttria stabilized zirconia (YSZ) obtained using mechanical alloying (MA). The as-milled powders show a body-centered cubic structure with grain sizes in the nanometer scale. After uniaxial pressing and sintering the compacts exhibit good mechanical properties. We discuss the correlation of these enhanced properties with the microstructural changes induced by heat treatment.
Elizabeth Refugio-García,José G. Miranda-Hernández,José A. Rodríguez-García,Enrique Rocha-Rangel 한양대학교 세라믹연구소 2011 Journal of Ceramic Processing Research Vol.12 No.3
The production of Al2O3/Ti/TiN functional materials through the nitriding in ammonia salts of previously Al2O3/Ti fabricated composites was achieved. The matrix for the preparation of the functional materials is to create an Al2O3-based composite that presents a fine and homogeneous dispersions of very fine metallic particles of Ti. After the nitriding, microstructural observations were carried out on the transverse zone of the materials’ surface showing the presence of a very thin film of about 20 μm of a constituent that was identified with the help of EDS as TiN. In addition, measurements of micro-hardness on the surface and in the core of the functional material were realized in order to determine the effect of nitride formation on the hardness.
Pavel Celis,Elisa Vazquez,Cintya G. Soria-Hernández,Diego Bargnani,Ciro A. Rodriguez,Elisabetta Ceretti,Erika García-López 한국정밀공학회 2022 International Journal of Precision Engineering and Vol.9 No.5
The use of nanoadditives in lubricants has gained much attention to the research community due to the enhancement of tribological properties and cooling capabilities. This paper studies the advantages of using a MQL (Minimum Quantity of Lubrication) system and nanoadditive in the manufacture of microneedle arrays in Ti6Al4V ELI alloy. Tungsten carbide ball nose tools with a cutting diameter of 200 μm were used in experimental tests. Surface and dimensional characterization was performed to evaluate the impact of a nanoadditive to a vegetable-based oil. Additionally, cutting forces and cutting edge radius (CER) were measured while needles were machined. Experimental tests confirmed that micro end milling with nanoadditives provide slightly better dimensional features and low cutting forces compared to oil. The performance of nanoadditives resulted in a reduction of surface roughness (~ 0.3 μm). Qualitative study of microneedles illustrated burr formation on needle surface manufactured without a nanoadditive solution. Results reveal an increment of CER using low feed rate values (2.0 μm/flute) while a reduction of CER was observed with feed rates up to 2.5 μm/flute. Our results indicated that the addition of nanoadditives to vegetable oil promotes a better product surface topography and cutting tool performance.