A Fast Electrical Energy Measurement Device and Power Consumption Detection Method of Electric Vehicle Charger

Zhao Fuping,Liu Rongmei,He Bei,Zheng Ke,Hu Xiaorui,Ji Jing,Hui Yan 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.9

More and more electric vehicles have been used in China, and many charging station has been built in China. Due to the reactive power loss caused by charging devices, the method to measure the power consumed by charging device is critical for power company. Based on impact loads generated by electric vehicles to the grid in fast charging mode, this paper studies the energy measurement method of electric vehicle charging mode. Since each phase current of charging post imbalance, to prevent the sum of each phase current vector of charging posts over the protection current threshold and the charging post breaker misusing in power circuit, preventing leakage circuit breaker current imbalance system is designed. Finally, this paper studies the power consumption detection methods of electric vehicle charging posts. By comparing the fast Fourier transform and wavelet analysis algorithms, an electric energy measurement device of electric vehicle charging machine based on FFT and wavelet analysis are proposed. Combined with fast data processing functions of DSP, the device can quickly and accurately measures electric vehicles charging amounts under different charging modes.

Complete Mitochondrial Genome Sequences of Chinese Indigenous Sheep with Different Tail Types and an Analysis of Phylogenetic Evolution in Domestic Sheep

Fan, Hongying,Zhao, Fuping,Zhu, Caiye,Li, Fadi,Liu, Jidong,Zhang, Li,Wei, Caihong,Du, Lixin Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.5

China has a long history of sheep (Ovis aries [O. aries]) breeding and an abundance of sheep genetic resources. Knowledge of the complete O. aries mitogenome should facilitate the study of the evolutionary history of the species. Therefore, the complete mitogenome of O. aries was sequenced and annotated. In order to characterize the mitogenomes of 3 Chinese sheep breeds (Altay sheep [AL], Shandong large-tailed sheep [SD], and small-tailed Hulun Buir sheep [sHL]), 19 sets of primers were employed to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) sequence of each breed. The sizes of the complete mitochondrial genomes of the sHL, AL, and SD breeds were 16,617 bp, 16,613 bp, and 16,613 bp, respectively. The mitochondrial genomes were deposited in the GenBank database with accession numbers KP702285 (AL sheep), KP981378 (SD sheep), and KP981380 (sHL sheep) respectively. The organization of the 3 analyzed sheep mitochondrial genomes was similar, with each consisting of 22 tRNA genes, 2 rRNA genes (12S rRNA and 16S rRNA), 13 protein-coding genes, and 1 control region (D-loop). The NADH dehydrogenase subunit 6 (ND6) and 8 tRNA genes were encoded on the light strand, whereas the rest of the mitochondrial genes were encoded on the heavy strand. The nucleotide skewness of the coding strands of the 3 analyzed mitogenomes was biased toward A and T. We constructed a phylogenetic tree using the complete mitogenomes of each type of sheep to allow us to understand the genetic relationships between Chinese breeds of O. aries and those developed and utilized in other countries. Our findings provide important information regarding the O. aries mitogenome and the evolutionary history of O. aries inside and outside China. In addition, our results provide a foundation for further exploration of the taxonomic status of O. aries.

Detection of copy number variation and selection signatures on the X chromosome in Chinese indigenous sheep with different types of tail

Zhu Caiye,Li Mingna,Qin Shizhen,Zhao Fuping,Fang Suli 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.9

Objective: Chinese indigenous sheep breeds can be classified into the following three categories by their tail morphology: fat-tailed, fat-rumped and thin-tailed sheep. The typical sheep breeds corresponding to fat-tailed, fat-rumped, and thin-tailed sheep are large-tailed Han, Altay, and Tibetan sheep, respectively. Detection of copy number variation (CNV) and selection signatures provides information on the genetic mechanisms underlying the phenotypic differences of the different sheep types. Methods: In this study, PennCNV software and F-statistics (FST) were implemented to detect CNV and selection signatures, respectively, on the X chromosome in three Chinese indigenous sheep breeds using ovine high-density 600K single nucleotide polymorphism arrays. Results: In large-tailed Han, Altay, and Tibetan sheep, respectively, a total of six, four and 22 CNV regions (CNVRs) with lengths of 1.23, 0.93, and 7.02 Mb were identified on the X chromosome. In addition, 49, 34, and 55 candidate selection regions with respective lengths of 27.49, 16.47, and 25.42 Mb were identified in large-tailed Han, Altay, and Tibetan sheep, respectively. The bioinformatics analysis results indicated several genes in these regions were associated with fat, including dehydrogenase/reductase X-linked, calcium voltage-gated channel subunit alpha1 F, and patatin like phospholipase domain containing 4. In addition, three other genes were identified from this analysis: the family with sequence similarity 58 member A gene was associated with energy metabolism, the serine/arginine-rich protein specific kinase 3 gene was associated with skeletal muscle development, and the interleukin 2 receptor subunit gamma gene was associated with the immune system. Conclusion: The results of this study indicated CNVRs and selection regions on the X chromosome of Chinese indigenous sheep contained several genes associated with various heritable traits.

Transparent organogel based on photopolymerizable magnetic cationic monomer for electromagnetic wave absorbing

Hengda Yuan,Yu Zhang,Guoqiang Lu,Fuping Chen,Tanlong Xue,Xin Shu,Yingying Zhao,Jun Nie,Xiaoqun Zhu 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.109 No.-

The application of conventional absorbing materials is limited due to complex preparation process andpoor transparency caused by fillers. In this study, a highly transparent ionic organogel was preparedby photocuring a dimethyl sulfoxide (DMSO) solution of a polymerizable magnetic cationic monomer[DAC]5[Dy(NCS)8]. Both components of organogel, which are the photopolymerizable magnetic cationicmonomer and polar solvent DMSO, have an important effect on the magnetic losses and dielectric lossesproperties of the organogel respectively. By tuning the mass ratio of DMSO to the monomer and the contentof the cross-linker, the complex permittivity of the gel could be effectively adjusted to improve theimpedance matching, and finally a gel with excellent wave absorption properties and good tensile propertieswas obtained. The optimum organogel was fabricated with a minimum reflection loss of 45.9 dBand a broadest effective absorption bandwidth (EAB) of up to 5.2 GHz, and effective absorption in the millimeterband (26.5–40 GHz) which is within the fifth generation (5G) mobile networks. With the advantagesof simple preparation method, arbitrary shape and good adhesion to a variety of substrates andcomplex surfaces, this multifunctional gel provides a new solution for complex scenarios requiring opticaltransparency and simultaneous absorption of electromagnetic waves.

Identifying long non-coding RNAs and characterizing their functional roles in swine mammary gland from colostrogenesis to lactogenesis

Shi Lijun,Zhang Longchao,Wang Ligang,Liu Xin,Gao Hongmei,Hou Xinhua,Zhao Fuping,Yan Hua,Cai Wentao,Wang Lixian 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.6

Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland. Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA). Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day –2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day –14, day –2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved. Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine. Objective: This study was conducted to identify the functional long non-coding RNAs (lncRNAs) for swine lactation by RNA-seq data of mammary gland.Methods: According to the RNA-seq data of swine mammary gland, we screened lncRNAs, performed differential expression analysis, and confirmed the functional lncRNAs for swine lactation by validation of genome wide association study (GWAS) signals, functional annotation and weighted gene co-expression network analysis (WGCNA).Results: We totally identified 286 differentially expressed (DE) lncRNAs in mammary gland at different stages from 14 days prior to (-) parturition to day 1 after (+) parturition, and the expressions of most of lncRNAs were strongly changed from day –2 to day +1. Further, the GWAS signals of sow milk ability trait were significantly enriched in DE lncRNAs. Functional annotation revealed that these DE lncRNAs were mainly involved in mammary gland and lactation developing, milk composition metabolism and colostrum function. By performing weighted WGCNA, we identified 7 out of 12 lncRNA-mRNA modules that were highly associated with the mammary gland at day –14, day –2, and day +1, in which, 35 lncRNAs and 319 mRNAs were involved.Conclusion: This study suggested that 18 lncRNAs and their 20 target genes were promising candidates for swine parturition and colostrum occurrence processes. Our research provided new insights into lncRNA profiles and their regulating mechanisms from colostrogenesis to lactogenesis in swine.

Function and Molecular Ecology Significance of Two Catechol-Degrading Gene Clusters in Pseudomonas putida ND6

( Sanyuan Shi ),( Liu Yang ),( Chen Yang ),( Shanshan Li ),( Hong Zhao ),( Lu Ren ),( Xiaokang Wang ),( Fuping Lu ),( Ying Li ),( Huabing Zhao ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.2

Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural environment over those with only a single copy. In the naphthalene-degrading strain Pseudomonas putida ND6, catechol can be converted into intermediates of the tricarboxylic acid cycle via either the ortho- or meta-cleavage pathways. In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catB_IC_IA_I and catB_IIC_IIA_II) on the chromosome play an important role. The cat_I and cat_II operons are co-transcribed, whereas catA_I and catA_II are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. In the presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatR_I and CatR_II occupy an additional downstream site, designated as the activation binding site. Notably, CatR_I binds to both the cat_I and cat_II promoters with high affinity, while CatR_II binds weakly. This is likely caused by a T to G mutation in the G/T-N11-A motif. Specifically, we found that CatR_I and CatR_II regulate catB_IC_IA_I and catB_IIC_IIA_II in a cooperative manner, which provides new insights into naphthalene degradation.