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Fu‑xiang Cao,Kun‑kun Deng,Cui‑ju Wang,Kai‑bo Nie,Wei Liang,Jian‑feng Fan 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.12
The 10 μm 10 vol% SiCp/2024Al composites sheet was successfully fabricated by semi-solid stir casting followed withtwo-step deformation (extrusion and rolling). The two-step thermal deformation solves the sheet rolling formability of theSiCp/2024Al composites produced by stir casting. Compared with the SiCp/2024Al composites prepared by traditional powdermetallurgy, this paper innovatively studied the synergistic enhancement between CuAl2phase and SiCpon the mechanicalproperties. The result shows that the two-step deformation can significantly improve the uniformity of the distribution of SiCpand CuAl2phases. At the same time, the average size of the SiCpand CuAl2phase decreases after the two-step deformation. During the hot extrusion, SiCphas an obvious hindrance to the refinement of the CuAl2phase. Moreover, the SiCp/2024Alcomposites sheet exhibits excellent yield strength (295 MPa), ultimate tensile strength (489 MPa), and elongation (10.42%). The performance improvement of SiCp/2024Al after two-step thermal deformation is mainly attributed to the improvedinterface bonding strength of SiCp-2024 Al matrix, the refined SiCpand the more uniform distribution of SiCp.
Expression profiles of circular RNAs in sheep skeletal muscle
Cao, Yang,You, Shuang,Yao, Yang,Liu, Zhi-Jin,Hazi, Wureli,Li, Cun-Yuan,Zhang, Xiang-Yu,Hou, Xiao-Xu,Wei, Jun-Chang,Li, Xiao-Yue,Wang, Da-Wei,Chen, Chuang-Fu,Zhang, Yun-Feng,Ni, Wei,Hu, Sheng-Wei Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10
Objective: Circular RNAs (circRNAs) are a newfound class of non-coding RNA in animals and plants. Recent studies have revealed that circRNAs play important roles in cell proliferation, differentiation, autophagy and apoptosis during development. However, there are few reports about muscle development-related circRNAs in livestock. Methods: RNA sequencing analysis was employed to identify and annotate circRNAs from longissimus dorsi of sheep. Reverse transcription followed by real-time quantitative (q) polymerase chain reaction (PCR) analysis verified the presence of these circRNAs. Targetscan7.0 and miRanda were used to analyse the interaction of circRNA-microRNA (miRNA). To investigate the function of circRNAs, an experiment was conducted to perform enrichment analysis hosting genes of circRNAs using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: About 75.5 million sequences were obtained from RNA libraries of sheep skeletal muscle. These sequences were mapped to 729 genes in the sheep reference genome. We identified 886 circRNAs, including numerous circular intronic RNAs and exonic circRNAs. Reverse transcription PCR (RT-PCR) and DNA sequencing analysis confirmed the presence of several circRNAs. Real-Time RT-PCR analysis exhibited resistance of sheep circRNAs to RNase R digestion. We found that many circRNAs interacted with muscle-specific miRNAs involved in growth and development of muscle, especially circ776. The GO and KEGG enrichment analysis showed that hosting genes of circRNAs was involved in muscle cell development and signaling pathway. Conclusion: The study provides comprehensive expression profiles of circRNAs in sheep skeletal muscle. Our study offers a large number of circRNAs to facilitate a better understanding of their roles in muscle growth. Meanwhile, we suggested that circ776 could be analyzed in future study.
Synthesis of graphene quantum dots from organic dye for the biosensor application
Li Qiuyan,Fu Jinrun,Chen Weifeng,Zhang Fatao,Cao Jie,Liu Xiang 한국탄소학회 2023 Carbon Letters Vol.33 No.4
How to effectively deal with the polluted water by the pollutant of organic dyes is the world problem. It is of great significance if the organic dyes in the polluted water can be directly turned into the useful materials through a facile approach. Herein, the water which contains the common organic dye, Reactive red 2 (RR2), has been chosen to be the model to synthesize graphene quantum dots (GQDs) by a facile route. The comprehensive characterizations, including TEM (HRTEM), XPS, Raman, PL and UV–Vis. spectra, have been performed to confirm the structures and explore the properties of the synthesized GQDs. Meanwhile, the excellent PL properties and low biotoxicity of the GQDs confer them with the potential applications in the biological fields. When the GQDs are excited by the wavelength of 360 nm, the maximum emission is achieved at 428 nm. It is well demonstrated that the synthesized GQDs are able to detect the Al3+ which causes multiple diseases, such as Parkinson, Alzheimer, kidney disease, and even cancer. The detection range is from 90 to 800 μM, which is different from the reported kinds of the literature. Therefore, this work not only provides an economical and environmental route on solving the universal problem from organic dyes, but also facilitates to advancing the synthesis and application of GQDs.
Qiang Xie,Hao Gao,Yuxin Ban,Xiang Fu,Xiaowen Liang,Zhilin Cao,Jun Duan 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11
To figure out the influence of soft-hard layer thickness ratio on specimens’ mechanical properties evolution and microcracking mechanism, combined with digital image correlation technology and acoustic emission facility, uniaxial compression tests were conducted on soft-hard interbedded rock-like materials considering seven bedding dip angles and two soft-hard layer thickness ratios. The results are as follows: 1) The failure mode and failure pattern of layered rock masses are mainly determined by the bedding dip angle α and are less related to the soft-hard thickness ratio. With the increase of dip angle, the failure strength curve of the specimen is approximately U-shaped, and the soft-hard layer thickness ratio affects the U-shaped. The decrease in soft-hard layer thickness ratio will reduce the uniaxial compressive strength of specimens with low dip angles (α≦30°), cause differences in the evolution path of local strain fields of layered rock masses and reduce the horizontal displacement of the main crack. 2) The increase of the proportion of soft layer in the soft-hard layer thickness ratio will reduce the proportion of tensile cracks in the tensile failure specimens and shear cracks in the shear failure specimens, complicating the process of cracks of the layer rock mass. 3) Increasing the proportion of soft layers in the soft-hard layers thickness ratio promotes the generation of secondary cracks in layered rock masses and further reduces the integrity of specimens. Finally, through the findings in this paper, it can provide a reference for analyzing the crack propagation behavior of layered rock.