The Influence of IgE on Cultured Human Mast Cells

Pernille Munk Frandsen,Inge Jacoba Maria Kortekaas Krohn,Hans Jürgen Hoffmann,Peter Oluf Schiøtz 대한천식알레르기학회 2013 Allergy, Asthma & Immunology Research Vol.5 No.6

Purpose: The mast cell plays a pivotal role in the human immune response. Crosslinking of 2 IgE molecules bound to the high affinity IgE receptor(FcεRI) on the surface of the mast cell results in mast cell degranulation and the release of several proinflammatory mediators. Patients with type-I allergyhave increased levels of IgE in the blood compared to healthy individuals. Methods: In a 6-week culture system of stem cells to human mastcells we investigated the effect of the concentration of IgE. The mast cells were cultured with different concentrations of IgE for the last 10 days ofthe maturation period. It was observed how the IgE concentration affects the histamine release, FcεRI density on the mast cell surface and the concentrationof other mediators. Results: A clear correlation between IgE concentration in culture medium and the release of histamine upon activationwas observed. It showed a bell-shaped dose response curve, with maximal response around an IgE-concentration of 250 ng/mL. Furthermore,the sensitivity of the mast cells and surface density of FcεRI on mast cell surface was also influenced by the IgE concentration in the culture medium. Conclusions: IgE in the culture medium during the last 10 days of mast cell maturation influences the release of the preformed mediator histamineafter mast cell activation and the density of FcεRI on the mast cell surface. The release of the de novo synthetized mediator prostaglandin D2and the expression of chymase and tryptase are not influenced by IgE in culture medium.

Survival analysis of endometrial cancer patients with cervical stromal involvement

Jonathan E. Frandsen,William T. Sause,Mark K. Dodson,Andrew P. Soisson,Thomas W. Belnap,David K. Gaffney 대한부인종양학회 2014 Journal of Gynecologic Oncology Vol.25 No.2

Objective: Stage II endometrial cancer is relatively uncommon. There is no consensus for appropriate adjuvant therapy in endometrial cancer patients with cervical stromal involvement (International Federation of Gynecology and Obstetrics [FIGO] stage II). This study investigates how adjuvant treatments and tumor characteristics influence overall survival (OS) and disease-free survival (DFS) in stage II patients in order to establish better treatment guidelines. Methods: This multi-institution, Institutional Review Board approved, study is a retrospective review of 40 endometrial cancer patients with cervical stromal involvement treated from 1993 to 2009. Kaplan-Meier estimates were used to evaluate OS and DFS. Results: OS was 85% at three years and 67% at five years. There were no significant differences in age, histology, depth of invasion, comorbid conditions, surgical staging or recurrence between patients who received radiation therapy (RT) and those who did not. However, patients with FIGO grade 1 cancers were less likely to receive RT (p=0.007). Patients treated with RT had a similar 5 year OS (n=33, 69%) to those treated with surgery only (n=7, 60%, p=0.746). There were no OS differences when evaluating by grade, histology, or depth of invasion between patients who did and did not receive RT. Four patients recurred: three were locoregional failures only, and one failed locally and distant. Conclusion: Patients receiving RT had higher grade tumors. Despite this, OS was comparable between the RT and the no RT cohorts. Local failure was the predominant pattern of failure. Endometrial cancer patients with cervical stromal involvement likely receive better locoregional control with the addition of adjuvant RT and we continue to advocate for RT in most cases.

Impact on survival with adjuvant radiotherapy for clear cell, mucinous, and endometriod ovarian cancer: the SEER experience from 2004 to 2011

Sagar C. Patel,Jonathan Frandsen,Sudershan Bhatia,David Gaffney 대한부인종양학회 2016 Journal of Gynecologic Oncology Vol.27 No.5

Objective: Evaluate the impact of radiotherapy on cause specific survival (CSS) and overallsurvival (OS) for stage (I–III) clear cell, mucinous, and endometriod ovarian cancer. Methods: We analyzed incidence, survival, and treatments from the Surveillance,Epidemiology, and End Results (SEER) Program from 2004 to 2011 for clear cell, mucinous,and endometriod histologies of the ovary for stages (I–III). We examined CSS and OS for allthree histologies combined and each histology with relation to the use of adjuvant radiationtherapy (RT). Survival analysis was calculated by Kaplan-Meier and log-rank analysis. Results: CSS was higher in individuals not receiving RT at 5 years (81% vs. 74%) and 10 years(74% vs. 65%, p=0.003). OS was higher in individuals not receiving RT at 5 years (76% vs. 73%) and 10 years (64% vs. 59%, p=0.039). Stage III patients receiving RT had a higher OS at5 years (54% vs. 44%) and 10 year intervals (36% vs. 30%, p=0.037). Stage III patients withmucinous histology receiving RT had a higher OS at 5 years (50% vs. 36%) and 10 years (45%vs. 26%, p=0.052). Conclusion: Those receiving RT had a lower CSS and OS at 5 and 10 years. However, subgroupanalysis revealed a benefit of RT in terms of OS for all stage III patients and for stage IIIpatients with mucinous histology.

Breast animation deformity

Diana Lydia Dyrberg,Camilla Bille,Gudjon Leifur Gunnarsson,Tove Faber Frandsen,C. Andrew Salzberg,Jens Ahm Sørensen,Jørn Bo Thomsen 대한성형외과학회 2019 Archives of Plastic Surgery Vol.46 No.1

Breast animation deformity (BAD) has been reported to occur after submuscular implant placement following breast augmentation and immediate breast reconstruction. Despite its apparent impact on patients’ quality of life, BAD has only recently become a topic of general concern. Its incidence and etiology have yet to be established. The aim of this systematic review was to identify papers that clearly defined and classified BAD and described how the degree of animation was assessed. We performed a search in PubMed and Embase. Studies meeting the inclusion criteria that described BAD after implant-based breast augmentation or immediate breast reconstruction were included. After screening 866 publications, four studies were included: three describing BAD after breast augmentation and one describing BAD after immediate breast reconstruction. The median percentage of patients with some degree of BAD was 58%. The highest percentages were found in patients operated on using the Regnault technique or the dual-plane technique (73%–78%). The lowest percentages were found following the dual-plane muscle-splitting technique (30%) and the triple-plane technique (33%). We found no studies meeting the inclusion criteria that analyzed BAD after prepectoral implant placement. This review of the current literature suggests that the degree of BAD is proportional to the degree of muscle involvement. Evidence is scarce, and the phenomenon seems to be underreported. Future comparative studies are warranted.

Breast animation deformity

Dyrberg, Diana Lydia,Bille, Camilla,Gunnarsson, Gudjon Leifur,Frandsen, Tove Faber,Salzberg, C. Andrew,Sorensen, Jens Ahm,Thomsen, Jorn Bo Korean Society of Plastic and Reconstructive Surge 2019 Archives of Plastic Surgery Vol.46 No.1

Breast animation deformity (BAD) has been reported to occur after submuscular implant placement following breast augmentation and immediate breast reconstruction. Despite its apparent impact on patients' quality of life, BAD has only recently become a topic of general concern. Its incidence and etiology have yet to be established. The aim of this systematic review was to identify papers that clearly defined and classified BAD and described how the degree of animation was assessed. We performed a search in PubMed and Embase. Studies meeting the inclusion criteria that described BAD after implant-based breast augmentation or immediate breast reconstruction were included. After screening 866 publications, four studies were included: three describing BAD after breast augmentation and one describing BAD after immediate breast reconstruction. The median percentage of patients with some degree of BAD was 58%. The highest percentages were found in patients operated on using the Regnault technique or the dual-plane technique (73%-78%). The lowest percentages were found following the dual-plane muscle-splitting technique (30%) and the triple-plane technique (33%). We found no studies meeting the inclusion criteria that analyzed BAD after prepectoral implant placement. This review of the current literature suggests that the degree of BAD is proportional to the degree of muscle involvement. Evidence is scarce, and the phenomenon seems to be underreported. Future comparative studies are warranted.

Multisite campaign on the open cluster M67 – III. δ Scuti pulsations in the blue stragglers

Bruntt, H.,Stello, D.,Suá,rez, J. C.,Arentoft, T.,Bedding, T. R.,Bouzid, M. Y.,Csubry, Z.,Dall, T. H.,Dind, Z. E.,Frandsen, S.,Gilliland, R. L.,Jacob, A. P.,Jensen, H. R.,Kang, Y. B.,Kim, S.-L. Blackwell Publishing Ltd 2007 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.378 No.4

<P>ABSTRACT</P><P>We have made an asteroseismic analysis of the variable blue stragglers in the open cluster M67. The data set consists of photometric time-series from eight sites using nine 0.6–2.1 m telescopes with a time-baseline of 43 d. In two stars, EW Cnc and EX Cnc, we detect the highest number of frequencies (41 and 26) detected in δ Scuti stars belonging to a stellar cluster, and EW Cnc has the second highest number of frequencies detected in any δ Scuti star. We have computed a grid of pulsation models that take the effects of rotation into account. The distribution of observed and theoretical frequencies shows that in a wide frequency range a significant fraction of the radial and non-radial low-degree modes are excited to detectable amplitudes. Despite the large number of observed frequencies we cannot constrain the fundamental parameters of the stars. To make progress we need to identify the degrees of some of the modes from either multicolour photometry or spectroscopy.</P>

Multisite campaign on the open cluster M67 – I. Observations and photometric reductions

Stello, D.,Arentoft, T.,Bedding, T. R.,Bouzid, M. Y.,Bruntt, H.,Csubry, Z.,Dall, T. H.,Dind, Z. E.,Frandsen, S.,Gilliland, R. L.,Jacob, A. P.,Jensen, H. R.,Kang, Y. B.,Kim, S.-L.,Kiss, L. L.,Kjeldsen, Blackwell Publishing Ltd 2006 MONTHLY NOTICES- ROYAL ASTRONOMICAL SOCIETY Vol.373 No.3

<P>ABSTRACT</P><P>We report on an ambitious multisite campaign aimed at detecting stellar variability, particularly solar-like oscillations, in the red giant stars in the open cluster M67 (NGC 2682). During the six-week observing run, which comprised 164 telescope nights, we used nine 0.6-m to 2.1-m class telescopes located around the world to obtain uninterrupted time series photometry. We outline here the data acquisition and reduction, with emphasis on the optimization of the signal-to-noise ratio of the low-amplitude (50–500 μmag) solar-like oscillations. This includes a new and efficient method for obtaining the linearity profile of the CCD response at ultrahigh precision (∼10 parts per million). The noise in the final time series is 0.50 mmag per minute integration for the best site, while the noise in the Fourier spectrum of all sites combined is 20 μmag. In addition to the red giant stars, this data set proves to be very valuable for studying high-amplitude variable stars such as eclipsing binaries, W UMa systems and δ Scuti stars.</P>