Communities of ammonia oxidizers at different stages of Spartina alterniflora invasion in salt marshes of Yangtze River estuary

Fei Xia,Jemaneh Zeleke,Qiang Sheng,Ji-Hua Wu,Zhe-Xue Quan 한국미생물학회 2015 The journal of microbiology Vol.53 No.5

Spartina alterniflora, an aggressive invasive plant species at the estuarine wetlands of China’s coasts, has become a major threat to the natural ecosystems. To understand its potential influence on nitrification processes, the community structures and abundance of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) were investigated using 454-pyrosequencing and quantitative real-time PCR (qPCR) in S. alterniflora invading salt marsh sediments at the Yangtze River estuary in Chongming island, Shanghai, China. Copy numbers of archaeal and bacterial ammonia monooxygenase subunit A (amoA) genes did not show accordant shifts with S. alterniflora invasion in the two sampling sites. However, the copy numbers of archaeal amoA gene were higher in summer than in spring. Phylogenetic analysis indicated that more than 90% of the archaeal and 92% of the bacterial amoA gene sequences were closely related to marine group I.1a and the clusters 13 and 15 in Nitrosospira lineage, respectively. The effect of different seasons (spring and summer) was important for the abundance variation of AOA, while different stages of S. alterniflora invasion did not show significant effect for both AOA and AOB. Variation of AOA community was significantly related to total carbon (TC) and sulfate concentration (P < 0.05), whereas the AOB community was significantly related to sulfate concentration, total nitrogen (TN), TC and pH (P < 0.05). In conclusion, the abundance and diversity of ammonia oxidizing microbial communities were not strongly affected by S. alterniflora invasion.

Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system

Fei Xia,Lina Wang,Jiayang Chen,Meng Fu,Guodong Wang,Yaping Yan,Langjun Cui 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

Aconitum carmichaeli Debx. (Ranunculaceae) is a potential source of an important herbal drug named “Fuzi”, which is derived from the lateral root of the plant. Increased therapeutic usage resulted in the great demand for artificial cultivation of A. carmichaeli, however, the obstacles caused by continuous cropping is a serious problem. Continuous cropping has shown to affect the soil biological and non-biological factors. The current study attempted to discover the variations of microbial communities and soil properties in shortterm continuous cropping of A. carmichaeli. An experimental procedure with A. carmichaeli planted two years continuously was established. The variation of the soil microbial community, disease incidence, soil properties, and the correlation between soil microbe and disease incidence were investigated. The disease incidence increased during the continuous cropping of A. carmichaeli. The PCoA and LefSe results indicated that fungal communities in rhizosphere soil were altered during the short-term continuous croppingand the bacterial community was disturbed by the cultivation of A. carmichaeli, however, in the following two years of continuous cropping period, the soil bacterial community has not changed obviously. Proportions of some fungal and bacterial genera were varied significantly (p < 0.05), and some genera of microflora showed a significant correlation with adisease incidence of A. carmichaeli. Microorganisms contributing to community composition discrepancy were also elucidated. Continuous cropping of A. carmichaeli disturbed the rhizosphere soil microbial community and altered the soil chemical parameters and soil pH. These variations in soil may be related to the occurrence of plant diseases. The current study will not only provide theoretical and experimental evidence for the A. carmichaeli continuous cropping obstacles but will also contribute to A. carmichaeli agricultural production and soil improvement.

Predictive Role of Computer Simulation in Assessing Signaling Pathways of Crizotinib-treated A549 Lung Cancer Cells

Xia, Pu,Mou, Fei-Fei,Wang, Li-Wei Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

Non-small-cell lung cancer (NSCLC) is a leading cause of cancer deaths worldwide. Crizotinib has been approved by the U.S. Food and Drug Administration for the treatment of patients with advanced NSCLC. However, understanding of mechanisms of action is still limited. In our studies, we confirmed crizotinib-induced apoptosis in A549 lung cancer cells. In order to assess mechanisms, small molecular docking technology was used as a preliminary simulation of signaling pathways. Interesting, our results of experiments were consistent with the results of computer simulation. This indicates that small molecular docking technology should find wide use for its reliability and convenience.

Analysis of differentially expressed proteins between the spinetoram-susceptible and -resistant strains of Plutella xylostella (L.)

Fei Yin,Qing Sheng Lin,Xia Feng,Huan-yu Chen,Zhen-yu Li,Zhen-di Hu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Plutella xylostella (L.), aworldwide vegetable pest, has developed resistance to spinetoram,whichwas previously effective for the control of P. xylostella (L.). The insecticidal resistance mechanismis essential to develop effective resistance management strategies. To explore the spinetoram resistance mechanism, a comparative proteomics approach was used to investigate the proteomic differences between the spinetoram-susceptible strain (SS) and spinetoram-resistant strain (RS) of P. xylostella (L.). Approximately 280 protein spotswere detected on each SDSPAGE gel. Of these, 19 proteinswere successfully identified byMALDI-TOF-MS.·Therewere 6 significantly downregulated spots and 13 up-regulated spots in RS, which showed significantly difference compared to that in SS. Based on the gene ontology(GO) system and KEGG database, the 19 identified proteins were classified into 6 groups includingmetabolisms, signal transduction, chaperones, transcriptional, protein synthesis, structural protein. Meanwhile, the expression profiles of 5 resistant related protein were further analysed by qRT-PCR. The results showed that 60S acidic ribosomal protein P2, glutathione S-transferase isozyme 3 and glutathione Stransferase deltawere significantly up-regulated,while phosphoglyceratemutase and receptor for activated protein kinase C homolog were significantly down-regulated. The expression tendency of mRNA was in accordance with which of protein. This study provided evidences that spinetoram induces proteomic changes in P. xylostella (L.), and it is contributed to help us understand the resistance mechanism of P. xylostella (L.) to spinetoram.

CO and C3H6 oxidation over La0.9Sr0.1CoO3 catalysts: Influence of preparation solvent

Fei Yan,Ping Li,Xia Zhang 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.5

High surface area powders, La0.9Sr0.1CoO3 (LSCO), were prepared by a hard template method with a template of SBA-15. In the preparation process, the solvent was ethanol, the mixture of ethanol and water, and water, respectively. Characterizations were performed to understand the properties of the catalysts. When the solvent is a mixture of ethanol and water, the prepared LSCO has the most easily extracted surface adsorbed oxygen in the H2 atmosphere, exhibiting the best light-off performances for CO oxidation. However, the LSCO prepared in ethanol solvent has the best lattice oxygen mobility, resulting in the best light-off performance for C3H6 oxidation. Moreover, the LSCO prepared in ethanol also shows the best catalytic activity for both CO and C3H6 oxidation under the conditions of simulated diesel exhaust. Furthermore, Pd was impregnated on the LSCO, which was prepared by a sol-gel method and a hard template method, separately; the later one shows higher CO and C3H6 catalytic oxidation activity.

Periodic Event-triggered State-feedback and Output-feedback Control for Linear Systems

Xia Chen,Fei Hao 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.4

This paper investigates the periodic event-triggered control for linear systems. Both state-feedback and output-feedback controllers are considered. The resulting closed-loop systems are modeled as delay systems and event conditions are constructed in more general forms. The stability analyses rely on the proposed Lyapunov functional and the improving free weighing matrix method. Criteria co-designing the feedback gain matrices and the event condition parameters are established to guarantee the asymptotic stability of the closed-loop systems. Moreover, we can obtain the maximum verifying period by solving a generalized eigenvalue problem. Simulation examples verify the less conservativeness and efficiency of the theoretical results.

Acid-doped polyaniline membranes for solar-driven interfacial evaporation

Xia Li,Dongmin Yue,Fei Liu,Jingtong Yu,Bing-Bing Li,De Sun,Xin Ma 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.1

Interface solar desalination technology is an important green and sustainable strategy to solve the freshwater crisis. Herein, polyaniline (PANI) membranes were prepared by immersion precipitation phase inversion (NIPs) method and using for solar-driven water evaporation. To improve the light absorption rate of the PANI membrane, acid doping modification was carried out to the membrane. The results show that the polyaniline-p-toluene sulfonic acid (PANI-PTSA) membrane modified by p-toluene sulfonic acid (PTSA) has microporous structure, and the hydrophilicity was greatly improved after modification. The water evaporation rate and solar energy conversion efficiency of PANI-PTSA membrane were 1.38 kg/(m2h) and 80.7% under 1 kW/m2 sunlight irradiation, respectively, significantly improved compared to the original membrane. Due to the electrostatic repulsion effect of PANI-PTSA on anion charged SO − 3 , Na+ is separated from Cl−, which reduces the salt crystallization in the evaporator, indicating that PANI-PTSA membrane has certain salt resistance in solar desalination experiments. This work provides a simple method to prepare the PANI-PTSA membrane with high efficiency and salt resistance that has huge potential for practical application of interface solar desalination technology.

Three new species of spiders of Erigoninae(Araneae:Linyphiidae)from China

Xia Qiang,Zhang Gu-Ren,Gao Jiu-Chun,Fei Rui,Joo-Pil Kim 한국거미연구소 2001 한국거미 Vol.17 No.2

Analysis of delay compensation in real-time dynamic hybrid testing with large integration time-step

Fei Zhu,Jin-Ting Wang,Feng Jin,Yao Gui,Meng-Xia Zhou 국제구조공학회 2014 Smart Structures and Systems, An International Jou Vol.14 No.6

With the sub-stepping technique, the numerical analysis in real-time dynamic hybrid testing is split into the response analysis and signal generation tasks. Two target computers that operate in real-time may be assigned to implement these two tasks, respectively, for fully extending the simulation scale of the numerical substructure. In this case, the integration time-step of solving the dynamic response of the numerical substructure can be dozens of times bigger than the sampling time-step of the controller. The time delay between the real and desired feedback forces becomes more striking, which challenges the well-developed delay compensation methods in real-time dynamic hybrid testing. This paper focuses on displacement prediction and force correction for delay compensation in the real-time dynamic hybrid testing with a large integration time-step. A new displacement prediction scheme is proposed based on recently-developed explicit integration algorithms and compared with several commonly-used prediction procedures. The evaluation of its prediction accuracy is carried out theoretically, numerically and experimentally. Results indicate that the accuracy and effectiveness of the proposed prediction method are of significance.

The Application of Decellularized Adipose Tissue Promotes Wound Healing

Xia Zenan,Guo Xiao,Yu Nanze,Zeng Ang,Si Loubin,Long Fei,Zhang Wenchao,Wang Xiaojun,Zhu Lin,Liu Zhifei 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6

Background: Due to adipose-derived stem cells (ASCs) being easy to obtain, their rapid proliferation rate, and their multidirectional differentiation capabilities, they have been widely used in the field of regenerative medicine. With the progress of decellularized adipose tissue (DAT) and adipose tissue engineering research, the role of DAT in promoting angiogenesis has gradually been emphasized. Methods: We examined the biological characteristics and biosafety of DAT and evaluated the stem cell maintenance ability and promotion of growth factor secretion through conducting in vitro and in vivo studies. Results The tested ASCs showed high rat:es of proliferation and adhered well to DAT. The expression levels of essential genes for cell stem maintenance, including OCT4, SOX2, and Nanog were low at 2–24 h and much higher at 48 and 96 h. The Adipogenic expression level of markers for ASCs proliferation including PPARγ, C/EPBα, and LPL increased from 2 to 96 h. Co-culture of ASCs and DAT increased the secretion of local growth factors, such as VEGF, PDGF-bb, bFGF, HGF, EGF, and FDGF-bb, and secretion gradually increased from 0 to 48 h. A model of full-thickness skin defects on the back of nude mice was established, and the co-culture of ASCs and DAT showed the best in vivo treatment effect. Conclusion: The application of DAT promotes wound healing, and DAT combined with ASCs may be a promising material in adipose tissue engineering and regenerative medicine.