Cloning and expression analysis of c-type and g-type lysozymes in yellow catfish (Pelteobagrus fulvidraco)

Fei Ke,Yun Wang,XiaoYao Cao,GaoWei Pan,Rui Geng 한국유전학회 2016 Genes & Genomics Vol.38 No.8

Lysozymes have important roles in innate immune system. Here, a c-type and a g-type lysozyme were identified from yellow catfish (Pelteobagrus fulvidraco). The deduced amino acid sequences of both lysozymes were conserved in catalytic sites and structural features as compared to their counterparts from other species. It was interesting that the g-type lysozyme possessed a signal peptide. The c-type and g-type lysozymes had the highest identity 89.4 and 76.2 % with that from channel catfish respectively. Phylogenetic analysis showed that the two lysozymes had a closely relationship with that from channel catfish and Astyanax mexicanus. Lysozymes from one order could form more than one clade in the phylogenetic tree, which indicated the gene duplications in evolution. Expression analysis with real time quantitative PCR revealed that the two lysozyme genes were constitutively expressed in all the tested tissues. The highest expression of c-type lysozyme was observed in liver, followed by spleen, head kidney, and trunk kidney, while the g-type lysozyme had highest expression in intestine, followed by spleen, head kidney, and trunk kidney. The mRNA levels of both genes were all up-regulated after challenging with Aeromonas hydrophila. However, there were differences in tissues and time points when the mRNA levels reached its peak between the two lysozymes. It indicated the diversity in regulation mechanisms and detailed functions among lysozymes. Taking together, these results will benefit the understanding of yellow catfish lysozymes.

Six new dammarane-type triterpene saponins from Panax ginseng flower buds and their cytotoxicity

Ke-Ke Li,Sha-Sha Li,Fei Xu,Xiao-Jie Gong 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.2

Background: Panax ginseng has been used for a variety of medical purposes in eastern countries formore than two thousand years. From the extensive experiences accumulated in its long medication usehistory and the substantial strong evidence in modern research studies, we know that ginseng hasvarious pharmacological activities, such as antitumor, antidiabetic, antioxidant, and cardiovascularsystemeprotective effects. The active chemical constituents of ginseng, ginsenosides, are rich instructural diversity and exhibit a wide range of biological activities. Methods: Ginsenoside constituents from P. ginseng flower buds were isolated and purified by variouschromatographic methods, and their structures were identified by spectroscopic analysis and comparisonwith the reported data. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromidemethod was used to test their cytotoxic effects on three human cancer cell lines. Results: Six ginsenosides, namely 6’emalonyl formyl ginsenoside F1 (1), 3beacetoxyl ginsenoside F1 (2),ginsenoside Rh24 (6), ginsenoside Rh25 (7), 7behydroxyl ginsenoside Rd (8) and ginsenoside Rh26 (10)were isolated and elucidated as new compounds, together with four known compounds (3e5 and 9). Inaddition, the cytotoxicity of these isolated compounds was shown as half inhibitory concentration values,a tentative structureeactivity relationship was also discussed based on the results of our bioassay. Conclusion: The study of chemical constituents was useful for the quality control of P. ginseng flowerbuds. The study on antitumor activities showed that new Compound 1 exhibited moderate cytotoxicactivities against HL-60, MGC80-3 and Hep-G2 with half inhibitory concentration values of 16.74, 29.51and 20.48 mM, respectively.

Energy factor of high-strength-steel frames with energy dissipation bays under repeated near-field earthquakes

Ke Ke,Michael C.H. Yam,Xu-Hong Zhou,Fuming Wang,Fei Xu 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.3

This investigation contributes to quantification of the inelastic seismic demands for high strength steel moment resisting frames equipped with energy dissipation bays (HSSF-EDBs) subjected to seismic sequences composed of repeated near-field ground motions. The emphasis is placed on the energy factor demand. A statistical examination of a database with more than eighty million energy factor demands of single-degree-of-freedom (SDOF) oscillators representing HSSF-EDBs responding in different yielding stages is conducted. The research findings show that in the damage-control stage, the energy factor which quantifies the peak seismic demand of a HSSF-EDB structure is insensitive to the repeated near-field earthquake motions. In contrast, a remarkable elevation of the energy factor is observed when oscillators characterising HSSF-EDBs progress into the ultimate stage. In addition, an increasing post-yielding stiffness ratio of the nonlinear force-displacement response in the damage-control stage may produce a detrimental effect on HSSF-EDBs progressing into the ultimate stage under repeated near-field earthquakes due to the corresponding evident increase of seismic demands. A nonlinear regression model quantifying the mean energy factor demand of the system under repeated near-field earthquake motions is proposed to facilitate performance-based seismic design of HSSF-EDBs.

Six new dammarane-type triterpene saponins from Panax ginseng flower buds and their cytotoxicity

Li, Ke-Ke,Li, Sha-Sha,Xu, Fei,Gong, Xiao-Jie The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.2

Background: Panax ginseng has been used for a variety of medical purposes in eastern countries for more than two thousand years. From the extensive experiences accumulated in its long medication use history and the substantial strong evidence in modern research studies, we know that ginseng has various pharmacological activities, such as antitumor, antidiabetic, antioxidant, and cardiovascular system-protective effects. The active chemical constituents of ginseng, ginsenosides, are rich in structural diversity and exhibit a wide range of biological activities. Methods: Ginsenoside constituents from P. ginseng flower buds were isolated and purified by various chromatographic methods, and their structures were identified by spectroscopic analysis and comparison with the reported data. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H- tetrazolium bromide method was used to test their cytotoxic effects on three human cancer cell lines. Results: Six ginsenosides, namely 6'-malonyl formyl ginsenoside F₁ (1), 3β-acetoxyl ginsenoside F₁ (2), ginsenoside Rh₂₄ (6), ginsenoside Rh₂₅ (7), 7β-hydroxyl ginsenoside Rd (8) and ginsenoside Rh₂₆ (10) were isolated and elucidated as new compounds, together with four known compounds (3-5 and 9). In addition, the cytotoxicity of these isolated compounds was shown as half inhibitory concentration values, a tentative structure-activity relationship was also discussed based on the results of our bioassay. Conclusion: The study of chemical constituents was useful for the quality control of P. ginseng flower buds. The study on antitumor activities showed that new Compound 1 exhibited moderate cytotoxic activities against HL-60, MGC80-3 and Hep-G2 with half inhibitory concentration values of 16.74, 29.51 and 20.48 μM, respectively.

Early Efficacy of Taxotere and Cisplatin Chemo-Radiotherapy for Advanced Cervical Cancer

Ke, Qing-Hua,Zhou, Shi-Qiong,Du, Wei,Lei, Yong,Huang, Min,Luo, Fei,Yang, Ji-Yuan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

The aim of this study was to investigate the early outcome of the taxotere and cisplatin chemoradiotherapy for advanced cervical cancer. Fifty-six cases (FIGO II b to IVa) were divided randomly into two groups: radiotherapy alone (28 cases) and radiation plus chemotherapy (TP) group. There was no difference in radiotherapy between the two groups. The RT+C cases who received TP regimen during the radiation, and DDP once weekly injection of vain, according to 20$mg/m^2$ and taxotere once weekly iv according to 35 $mg/m^2$. These regimens were given for 4~5weeks, and some medicines to control vomiting were available for the RT+C cases. The two groups received an oral medicine MA 160mg every day during the treatment. Regarding early outcome, the complete remission rate was 64.3% and partial remission rate was 35.7% in RT+C. The complete remission rate was 32.1% and partial remission rate was 39.3% in RT. The total response rate and complete remission in the RT+C group were higher than that in the RT group. We conclude that taxotere and cisplatin chemoradiotherapy can improve the early outcome of the advanced cervical cancer, the adverse effects being endurable.

Glacier Change in the Yigong Zangbo Basin, Tibetan Plateau, China

Ke, Chang-Qing,Lee, Hoonyol,Han, Yan-Fei The Korean Society of Remote Sensing 2019 大韓遠隔探査學會誌 Vol.35 No.4

Distinguishing debris-covered glaciers from debris-free glaciers is difficult when using only optical remote sensing images to extract glacier boundaries.According to the features that the surface temperature of debris-covered glacier is lower than surrounding objects, and higher than clean glaciers, glacial changes in the Yigong Zangbo basin was analyzed on the basis of visible, near-infrared and thermal-infrared band images of Landsat TM and OLI/TIRS in the support of ancillary digital elevation model (DEM). The results indicated that glacier area gradually declined from $928.76km^2$ in 1990 to $918.46km^2$ in 2000 and $901.51km^2$ in 2015. However, debris-covered glacier area showed a slight increase from $63.39km^2$ in 1990 to $66.24km^2$ in 2000 and $71.16km^2$ in 2015. During 25 years, the glacier length became shorter continuously with terminus elevation rising up. The area of moraine lakes in 1990 was $1.43km^2$, which increased to $1.98km^2$ in 2000 and $3.41km^2$ in 2015. In other words, the total area of the moraine lakes in 2015 is 2.38 times of that in 1990. This increase in moraine lake area could be the result of accelerated glacier melt and retreat, which is consistent with the significant warming trend in recent decades in the basin.

Calculation of Vibrational Relaxation Times in Multi-component Excitable Gases

Ke-Sheng Zhang,Weihua Ou,Xueqin Jiang,Fei Long,Mingzhe Hu 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.7

In the research field of acoustic propagation in excitable gases, one of the most critical parametersis the vibrational relaxation time, which determines the frequency of the acoustic dispersion stepor the absorption maximum. In this paper, the vibrational relaxation equations given by Tanczos[J. Chem. Phys. 25, 439 (1956)] have been applied to calculate the vibrational multi-relaxationtimes in multi-component gases. The eigenvalues of the energy-transition-rate matrix are provento be the reciprocals of the multi-relaxation times. Comparisons demonstrate that our relaxationfrequencies calculated for various gas compositions, including carbon dioxide, methane, chlorine,nitrogen, and oxygen, agree with the experimental data.

Improved Fiber Uniformity and Jet Number in Multi-spinneret Electrospinning via Auxiliary Electrode

Yu-Ke Wu,Zong-Jie Li,Jie Fan,Liang Wang,Peng-Fei Zhang,Jian Liu,Yong Liu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.6

Auxiliary-electrode electrospinning process is a novel technique to improve nanofiber production rate Due to theformation of multiple jets. In the present work, dual-spinneret (DSE) and tri-spinneret electrospinning (TSE) with anauxiliary electrode were employed to evaluate the number of jets and fiber uniformity in this electrospinning process. Theresults showed that all the spinnerets in DSE and TSE with an auxiliary electrode could produce 2-12 jets, which could beadjusted by controlling the process parameters such as applied voltage and injection speed. The number of jets initiallyincreased and then decreased with a rise in applied voltage in these two systems. Moreover, the produced nanofibers weresmaller in size and possessed a more uniform fiber diameter per distribution in comparison to those without the auxiliaryelectrode. This work provides a new insight on mass production of nanofibers and designing electrospinning apparatus.

Long Noncoding RNA NEAT1 Aggravates Aβ-Induced Neuronal Damage by Targeting miR-107 in Alzheimer’s Disease

Sha Ke,Zhaohui Yang,Fei Yang,Xiaoming Wang,Juan Tan,Bo Liao 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.7

Purpose: Alzheimer’s disease (AD) is the most common neurodegenerative disease, with a rising prevalence worldwide. Longnoncoding RNAs (lncRNAs) have been found to play important roles in the development and treatment of AD. However, the exactrole of lncRNA nuclear enriched abundant transcript 1 (NEAT1) in neuronal damage in AD is largely unknown. Materials and Methods: The AD model was established in SH-SY5Y and SK-N-SH cells via treatment with amyloid β1-42 (Aβ). Theexpression of NEAT1 and microRNA-107 (miR-107) was measured by quantitative real-time polymerase chain reaction. Cell viabilityand apoptosis were detected by MTT assay, immunocytochemistry, and flow cytometry. The expression of phosphorylated tauprotein (p-Tau) was measured by Western blot. The interaction between NEAT1 and miR-107 was explored by bioinformaticsanalysis, luciferase activity, and RNA immunoprecipitation assays. Results: NEAT1 expression was enhanced in Aβ-treated SH-SY5Y and SK-N-SH cells, and its knockdown attenuated Aβ-inducedinhibition of viability and promotion of apoptosis and p-Tau levels. NEAT1 was indicated as a decoy of miR-107. miR-107 abundancewas reduced in Aβ-treated cells, and its overexpression reversed Aβ-induced injury. Moreover, interference of miR-107 abatedsilencing of NEAT1-mediated inhibition of neuronal damage in Aβ-treated SH-SY5Y and SK-N-SH cells. Conclusion: LncRNA NEAT1 aggravated Aβ-induced neuronal damage by sponging miR-107, indicating a novel avenue for treatmentof AD.

Expression and Immunogenicity of SARSCoV-2 Virus-Like Particles based on Recombinant Truncated HEV-3 ORF2 Capsid Protein

( Yong-fei Zhou ),( Jiao-jiao Nie ),( Chao Shi ),( Ke Ning ),( Yu-feng Cao ),( Yanbo Xie ),( Hongyu Xiang ),( Qiuhong Xie ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

COVID-19 is an emerging disease that poses a severe threat to global public health. As such, there is an urgent demand for vaccines against SARS-CoV-2, the virus that causes COVID-19. Here, we describe a virus-like nanoparticle candidate vaccine against SARS-CoV-2 produced by an E. coli expression system. The fusion protein of a truncated ORF2-encoded protein of aa 439~608 (p170) from hepatitis E virus CCJD-517 and the receptor-binding domain of the spike protein from SARSCoV-2 were expressed, purified and characterized. The antigenicity and immunogenicity of p170-RBD were evaluated in vitro and in Kunming mice. Our investigation revealed that p170-RBD selfassembled into approximately 24 nm virus-like particles, which could bind to serum from vaccinated people (p < 0.001) and receptors on cells. Immunization with p170-RBD induced the titer of IgG antibody vaccine increased from 14 days post-immunization and was significantly enhanced after a booster immunization at 28 dpi, ultimately reaching a peak level on 42 dpi with a titer of 4.97 log10. Pseudovirus neutralization tests showed that the candidate vaccine induced a strong neutralizing antibody response in mice. In this research, we demonstrated that p170-RBD possesses strong antigenicity and immunogenicity and could be a potential candidate for use in future SARS-CoV-2 vaccine development.