Distinctive Roles of Wnt Signaling in Chondrogenic Differentiation of BMSCs under Coupling of Pressure and Platelet-Rich Fibrin

Cheng Baixiang,Feng Fan,Shi Fan,Huang Jinmei,Zhang Songbai,Quan Yue,Tu Teng,Liu Yanli,Wang Junjun,Zhao Ying,Zhang Min 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.4

BACKGROUND: Although newly formed constructs of feasible pressure-preadjusted bone marrow mesenchymal stem cells (BMSCs) and platelet-rich fibrin (PRF) showed biomechanical flexibility and superior capacity for cartilage regeneration, it is still not very clear how BMSCs and seed cells feel mechanical stimuli and convert them into biological signals, and the difference in signal transduction underlying mechanical and chemical cues is also unclear. METHODS: To determine whether mechanical stimulation (hydrostatic pressure) and chemical cues (platelet-rich fibrin, PRF) activate canonical or noncanonical Wnt signaling in BMSCs, BMSCs cocultured with PRF were subjected to hydrostatic pressure loading, and the activation of the Wnt signaling molecules and expression of cartilage-associated proteins and genes were determined by western blotting and polymerase chain reaction (PCR). Inhibitors of canonical or noncanonical Wnt signaling, XVX-939 or L690,330, were adopted to investigate the role of Wnt signaling molecules in mechanically promoted chondrogenic differentiation of BMSCs. RESULTS: Hydrostatic pressure of 120 kPa activated both Wnt/b-catenin signaling and Wnt/Ca2? signaling, with the the maximum promotion effect at 60 min. PRF exerted no synergistic effect on Wnt/b-catenin signaling activation. However, the growth factors released by PRF might reverse the promotion effects of pressure on Wnt/Ca2? signaling. Real-time PCR and Western blotting results showed that pressure could activate the expression of Col-II, Sox9, and aggrecan in BMSCs cocultured with PRF. Blocking experiment found a positive role of Wnt/b-catenin signaling, and a negative role of Wnt/ Ca2? signaling in chondrogenic differentiation of the BMSCs. Mutual inhibition exists between canonical and noncanonical Wnt signaling in BMSCs under pressure. CONCLUSION: Wnt signaling participates in the pressure-promoted chondrogenesis of the BMSCs co-cultured with PRF, with canonical and noncanonical pathways playing distinct roles during the process.

In-package inhibition of <i>E. coli</i> O157:H7 on bulk Romaine lettuce using cold plasma

Min, Sea C.,Roh, Si Hyeon,Niemira, Brendan A.,Boyd, Glenn,Sites, Joseph E.,Uknalis, Joseph,Fan, Xuetong Elsevier 2017 Food microbiology Vol.65 No.-

<P><B>Abstract</B></P> <P>Dielectric barrier discharge atmospheric cold plasma (DACP) treatment was evaluated for the inactivation of <I>Escherichia coli</I> O157:H7, surface morphology, color, carbon dioxide generation, and weight loss of bulk Romaine lettuce in a commercial plastic clamshell container. The lettuce samples were packed in a model bulk packaging configuration (three rows with either 1, 3, 5, or 7 layers) in the container and treated by DACP (42.6 kV, 10 min). DACP treatment reduced the number of <I>E. coli</I> O157:H7 in the leaf samples in the 1-, 3-, and 5-layer configurations by 0.4–0.8 log CFU/g lettuce, with no significant correlation to the sample location (<I>P</I> > 0.05). In the largest bulk stacking with 7 layers, a greater degree of reduction (1.1 log CFU/g lettuce) was observed at the top layer, but shaking the container increased the uniformity of the inhibition. DACP did not significantly change the surface morphology, color, respiration rate, or weight loss of the samples, nor did these properties differ significantly according to their location in the bulk stack. DACP treatment inhibited <I>E. coli</I> O157:H7 on bulk lettuce in clamshell containers in a uniform manner, without affecting the physical and biological properties and thus holds promise as a post-packaging process for fresh and fresh-cut fruits and vegetables.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Dielectric barrier atmospheric cold plasma (DACP) was applied to <I>E. coli</I> O157:H7. </LI> <LI> DACP inhibited <I>E. coli</I> O157:H7 on bulk lettuce layered in a commercial container. </LI> <LI> DACP uniformly inhibited the pathogen on 3- and 5-layer cut lettuce. </LI> <LI> Shaking container ensured inhibition uniformity in the 7-layer lettuce sample. </LI> <LI> Sensory and metabolic properties of the bulk lettuce were unaffected by DACP. </LI> </UL> </P>

TRANSLATION OF THE CONFUCIAN CONCEPTS IN THE ANALECTS: A SOCIOLINGUISTIC PERSPECTIVE

FAN MIN 한국외국어대학교 통번역연구소 2019 한국외국어대학교 통번역연구소 학술대회 Vol.2019 No.07

Inactivation of Escherichia coli O157:H7 and Aerobic Microorganisms in Romaine Lettuce Packaged in a Commercial Polyethylene Terephthalate Container Using Atmospheric Cold Plasma

MIN, SEA C.,ROH, SI HYEON,BOYD, GLENN,SITES, JOSEPH E.,UKNALIS, JOSEPH,FAN, XUETONG,NIEMIRA, BRENDAN A. ational Association for Food Protection 2017 Journal of food protection Vol.80 No.1

<B>ABSTRACT</B><P>The effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of Escherichia coli O157:H7 and aerobic microorganisms in romaine lettuce packaged in a conventional commercial plastic container were evaluated during storage at 4°C for 7 days. Effects investigated included the color, carbon dioxide (CO2) generation, weight loss, and surface morphology of the lettuce during storage. Romaine lettuce pieces, with or without inoculation with a cocktail of three strains of E. coli O157:H7 (~6 log CFU/g of lettuce), were packaged in a polyethylene terephthalate commercial clamshell container and treated at 34.8 kV at 1.1 kHz for 5 min by using a DACP treatment system equipped with a pin-type high-voltage electrode. Romaine lettuce samples were analyzed for inactivation of E. coli O157:H7, total mesophilic aerobes, and yeasts and molds, color, CO2 generation, weight loss, and surface morphology during storage at 4°C for 7 days. The DACP treatment reduced the initial counts of E. coli O157:H7 and total aerobic microorganisms by ~1 log CFU/g, with negligible temperature change from 24.5 ± 1.4°C to 26.6 ± 1.7°C. The reductions in the numbers of E. coli O157:H7, total mesophilic aerobes, and yeasts and molds during storage were 0.8 to 1.5, 0.7 to 1.9, and 0.9 to 1.7 log CFU/g, respectively. DACP treatment, however, did not significantly affect the color, CO2 generation, weight, and surface morphology of lettuce during storage (P &gt; 0.05). Some mesophilic aerobic bacteria were sublethally injured by DACP treatment. The results from this study demonstrate the potential of applying DACP as a postpackaging treatment to decontaminate lettuce contained in conventional plastic packages without altering color and leaf respiration during posttreatment cold storage.</P>

In-package atmospheric cold plasma treatment of bulk grape tomatoes for microbiological safety and preservation

Min, Sea C.,Roh, Si Hyeon,Niemira, Brendan A.,Boyd, Glenn,Sites, Joseph E.,Fan, Xuetong,Sokorai, Kimberly,Jin, Tony Z. Elsevier 2018 Food Research International Vol.108 No.-

<P><B>Abstract</B></P> <P>Effects of dielectric barrier discharge atmospheric cold plasma (DACP) treatment on the inactivation of <I>Salmonella</I> and the storability of grape tomato were investigated. Grape tomatoes, with or without inoculation with <I>Salmonella</I>, were packaged in a polyethylene terephthalate (PET) commercial clamshell container and cold plasma-treated at 35 kV at 1.1 A for 3 min using a DACP system equipped with a pin-type high-voltage electrode. DACP treatment inactivated <I>Salmonella</I> (<I>p</I> < 0.05) without altering the color or firmness of the grape tomatoes (<I>p</I> > 0.05). DACP treatment inactivated <I>Salmonella</I> uniformly in both layers of the double-layer configuration of the grape tomatoes regardless of the position of the tomatoes in each layer. <I>Salmonella</I> was most efficiently inactivated when the headspace to tomato volume ratio of the container was highest. Integration of rolling of tomatoes during treatment significantly increased the <I>Salmonella</I> reduction rates from 0.9 ± 0.2 log CFU/tomato to 3.3 ± 0.5 log CFU/tomato in the double-layer configuration of the tomato samples. Rolling-integrated DACP also initially reduced the number of total mesophilic aerobic bacteria and yeast and molds in the double-layer configuration of tomato samples by 1.3 ± 0.3 and 1.5 ± 0.2 log CFU/tomato, respectively. DACP treatment effectively reduced the growth of <I>Salmonella</I> and indigenous microorganisms at 10 and 25 °C, and did not influence the surface color, firmness, weight loss, lycopene concentration and residual ascorbic acid of grape tomatoes during storage at 10 and 25 °C. DACP treatment holds promise as a post-packaging process for improving microbial safety against <I>Salmonella</I> and storability of fresh grape tomatoes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Atmospheric cold plasma treatment (ACPT) inactivates <I>Salmonella</I> on grape tomatoes. </LI> <LI> A higher container headspace to tomato volume ratio results in higher inactivation. </LI> <LI> Inactivation is uniform with tomatoes in a 2-layer configuration in the container. </LI> <LI> Rolling of samples during ACPT increases <I>Salmonella</I> reduction rates. </LI> <LI> ACPT inhibits microbial growth during storage without affecting quality. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Controlling robot by image-based visual servoing with stereo cameras

Fan Jun-Min,Won Sang-Chul 한국정보기술응용학회 2005 한국정보기술응용학회학술대회 Vol.2005 No.1

The bHLH Transcription Factor HBI1 Mediates the Trade-Off between Growth and Pathogen-Associated Molecular Pattern–Triggered Immunity in <i>Arabidopsis</i>

Fan, Min,Bai, Ming-Yi,Kim, Jung-Gun,Wang, Tina,Oh, Eunkyoo,Chen, Lawrence,Park, Chan Ho,Son, Seung-Hyun,Kim, Seong-Ki,Mudgett, Mary Beth,Wang, Zhi-Yong American Society of Plant Biologists 2014 The Plant cell Vol.26 No.2

<P>HBI1 is activated posttranscriptionally by growth-promoting hormonal and environmental signals through the triple-HLH/bHLH cascade but is repressed transcriptionally by pathogen-associated molecular pattern signals. HBI1 both activates growth and inhibits immunity, thereby acting as a crosstalk node that mediates the trade-off between growth and immunity.</P>

Vitexicarpin Induces Apoptosis in Human Prostate Carcinoma PC-3 Cells through G2/M Phase Arrest

Meng, Fan-Min,Yang, Jing-Bo,Yang, Chun-Hui,Jiang, Yu,Zhou, Yong-Feng,Yu, Bo,Yang, Hong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

Vitexicarpin (3', 5-dihydroxy-3, 4', 6, 7-tetramethoxyflavone), a polymethoxyflavone isolated from Viticis Fructus (Vitex rotundifolia Linne fil.), has long been used as an anti-inflammatory herb in traditional Chinese medicine. It has also been reported that vitexicarpin can inhibit the growth of various cancer cells. However, there is no report elucidating its effect on human prostate carcinoma cells. The aim of the present study was to examine the apoptotic induction activity of vitexicarpin on PC-3 cells and molecular mechanisms involved. MTT studies showed that vitexicarpin dose-dependently inhibited growth of PC-3 cells with an $IC_{50}{\sim}28.8{\mu}M$. Hoechst 33258 staining further revealed that vitexicarpin induced apoptotic cell death. The effect of vitexicarpin on PC-3 cells apoptosis was tested using prodium iodide (PI)/Annexin V-FITC double staining and flow cytometry. The results indicated that vitexicarpin induction of apoptotic cell death in PC-3 cells was accompanied by cell cycle arrest in the G2/M phase. Furthermore, our study demonstrated that vitexicarpin induction of PC-3 cell apoptosis was associated with upregulation of the proapoptotic protein Bax, and downregulation of antiapoptotic protein Bcl-2, release of Cytochrome c from mitochondria and decrease in mitochondrial membrane potential. Our findings suggested that vitexicarpin may become a potential leading drug in the therapy of prostate carcinoma.

Allergen-Dependent Differences in ILC2s Frequencies in Patients With Allergic Rhinitis

Dachuan Fan,Xiangdong Wang,Min Wang,Yang Wang,Liang Zhang,Ying Li,Erzhong Fan,Feifei Cao,Koen Van Crombruggen,Luo Zhang 대한천식알레르기학회 2016 Allergy, Asthma & Immunology Research Vol.8 No.3

Purpose: Group 2 innate lymphoid cells (ILC2s) are a novel population of lineage-negative cells that induce innate type 2 responses by producing the critical Th2-type cytokines IL-5 and IL-13 in response to IL-25 and IL-33 stimulation. ILC2s accumulation in the peripheral blood of patients with allergic rhinitis (AR) is controversial; the precise role of ILC2s in the immunopathogenesis of AR is still not clear. We investigated the role of ILC2s in phenotypic AR sensitized to distinct allergens. Methods: Flow cytometric analysis of the peripheral blood of 7 healthy controls (HCs), 9 patients monosensitized to house dust mite (HDM), and 8 patients monosensitized to mugwort was performed to quantify ILC2s frequency. Peripheral blood mononuclear cells (PBMCs) were isolated from HDM-AR and mugwort-AR patients, and Lineage- and Lineage+ cells were separated using a fluorescence-activated cell sorter (FACS). IL-5 and IL-13 levels in the supernatants of PBMCs, and Lineage- and Lineage+ cells stimulated with IL-25 and/or IL-33 combined with IL-2 in vitro were assessed using the Milliplex magnetic bead kit. Results: The percentage of ILC2s was significantly elevated in HDM-AR patients compared to mugwort-AR patients and HCs, while no significant difference was found between mugwort-AR patients and HCs. IL-33±IL-25 plus IL-2 induced a significantly greater release of IL-5 and IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. IL-25 plus IL-2 also induced a significantly greater release of IL-13 in the PBMCs of HDM-AR patients compared to PBMCs of mugwort-AR patients. Stimulation with IL-33 and/or IL-25 combined with IL-2 also induced a significantly greater IL-5 and IL-13 release from Lineage- cells compared to Lineage+ cells. Conclusions: AR patients sensitized to HDM or mugwort allergen have distinct phenotypic and functional profiles in ILC2s frequencies. ILC2s mediate major type 2 immunity in the development of HDM-AR and may be a potential therapeutic target.

한라골드 수확기 결정을 위한 생리적 지표 비교

범수정(Xiujing Fan),임찬규(Chan Kyu Lim),민정호(Jeong Ho Min),배태민(Tae Min Bae),김진국(Jin Gook Kim),황용수(Yong-Soo Hwang) 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.5