http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Long-Term Follow-up of Enhanced Holter- Electrocardiography Monitoring in Acute Ischemic Stroke
Rolf Wachter,Mark Weber-Krüger,Gerhard F. Hamann,Pawel Kermer,Jan Liman,Meinhard Mende,Joachim Seegers,Katrin Wasser,Sonja Gröschel,Timo Uphaus,Holger Poppert,Martin Köhrmann,Markus Zabel,Ulrich Laufs 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.1
Background and Purpose Prolonged electrocardiography (ECG)-monitoring in stroke patients improves the detection of paroxysmal atrial fibrillation (pAF). However, most randomized studies only had short follow-up. We aimed to provide 3-year follow-up data for AF detection and stroke recurrence risk. Methods We randomized 402 patients aged ≥60 years with acute ischemic strokes without AF to either enhanced and prolonged monitoring (EPM; 3×10-day Holter-ECG-monitoring) or standard-of-care (≥24 hours ECG-monitoring). The endpoint of the current analysis was AF within 36 months analyzed by intention to treat. Long-term follow-up was performed for 36 months. Results Two hundred and seventy-four patients (80%) participated in the extended follow-up(median duration of follow-up was 36 months [interquartile range, 12 to 36]). During the first 6 months, more AF was documented in the EPM arm compared to the control arm (13.5% vs. 5.1%; 95% confidence interval, 2.9% to 14.4%; P=0.004). During months 6 to 36, AF was less detected in the EPM intervention arm than in the control arm (2.0% vs. 7.3%; 95% confidence interval, 0.7% to 9.9%; P=0.028). Overall, the detection rate of AF within 36 months was numerically higher within the EPM group (15.0% vs. 11.1%, P=0.30). Numerically less patients in the EPM arm had recurrent ischemic strokes (5.5% vs. 9.1%, P=0.18), transient ischemic attacks (3.0% vs. 4.5%, P=0.44) or died (4.5% vs. 6.6%, P=0.37). Conclusions Enhanced and prolonged ECG monitoring increased AF detection during the first six months, but there was significantly more clinical AF during months 6 to 36 observed in the usual-care arm. This suggests that EPM leads to an earlier detection of clinically relevant AF.
쥐의 골수로부터 추출한 줄기세포를 이용한 조골세포로의 분화 유도과정에서 나타난 문제점에 관한 분석 연구
김인숙,조태형,장옥련,이규백,박용두,노인섭,이종호,김명진,황순정,Kim, In-Sook,Cho, Tae-Hyung,Zhang, Yu-Lian,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Lee, Jong-Ho,Kim, Myung-Jin,Hwang, Soon-Jung 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1
This study was aimed to characterize osteogenic potential of rat bone marrow stromal cells (BMSC) isolated with standard flushing method and investigate the plasticity of transdifferentiation between osteoblastic and adipocytic lineage of cultured BMSC. Unlike aspiration method in human, rat bone marrow was extracted by means of irrigation with culture media that elevates the possibility of co-extraction of committed osteoprogenitor, or preosteoblast or other progenitor cells of several types present inside bone marrow. The cultured stromal cells showed high ALP activity which is representative marker of osteoblast without any treatment. Osteogenic inducers such as Dex and BMP-2 were examined for the evaluation of their effect on osteogenic and adipocytic differentiation of stromal cells, because they function as osteoinductive agent in stromal cells, but simultaneously induce adipogenic differentiation. Osteogenic differentiation was evaluated by measuring alkaline phosphatase activity or mRNA expression of osteoblast markers such as osteopontin, bone sialoprotein, collagen type I and CbfaI, and in vitro matrix mineralization by von Kossa staining. Oil red staining method was used to detect adipocyte and adipocytic marker, aP2 and $PPAR{\gamma}2$ expression was examined using RT-PCR. It can be supposed that irrigation procedure resulted in high portion of already differentiation-committed osteoprogenitor cell showing elevated ALP activity and strong mineralization only under the supplement of $100{\mu}M$ ascorbic 2-phosphate and 10mM ${\beta}$-glycerophosphate without any treatment of osteogenic inducers such as Dex and BMP-2. Dex and BMP-2 seemed to transdifferentiate osteoprogenitor cells having high ALP activity into adipocytes temporarily, but continuous treatment redifferentiated into osteoblast and developed in vitro matrix mineralization. This property must be considered either in tissue engineering for bone regeneration, or in research of characterization of osteogenic differentiation, with rat BMSC isolated by the standard irrigation method.
Complex Multicolor Tilings and Critical Phenomena in Tetraphilic Liquid Crystals
Zeng, X.,Kieffer, R.,Glettner, B.,Nurnberger, C.,Liu, F.,Pelz, K.,Prehm, M.,Baumeister, U.,Hahn, H.,Lang, H.,Gehring, G. A.,Weber, C. H. M.,Hobbs, J. K.,Tschierske, C.,Ungar, G. American Association for the Advancement of Scienc 2011 Science Vol.331 No.6022
<P>T-shaped molecules with a rod-like aromatic core and a flexible side chain form liquid crystal honeycombs with aromatic cell walls and a cell interior filled with the side chains. Here, we show how the addition of a second chain, incompatible with the first (X-shaped molecules), can form honeycombs with highly complex tiling patterns, with cells of up to five different compositions ('colors') and polygonal shapes. The complexity is caused by the inability of the side chains to separate cleanly because of geometric frustration. Furthermore, a thermoreversible transition was observed between a multicolor (phase-separated) and a single-color (mixed) honeycomb phase. This is analogous to the Curie transition in simple and frustrated ferro- and antiferromagnets; here spin flips are replaced by 180° reorientations of the molecules.</P>
사람의 골수 줄기 세포로부터의 골세포 분화 과정에서 BMP-2가 미치는 영향과 그에 따른 분화 유전자의 발현 비교 연구
김인숙,장옥련,조태형,이규백,박용두,노인섭,황순정,김명진,이종호,Kim, In-Sook,Zhang, Yu-Lian,Cho, Tae-Hyung,Lee, Kyu-Back,Park, Yong-Doo,Rho, In-Sub,Weber, F.,Hwang, Soon-Jung,Kim, Myung-Jin,Lee, Jong-Ho 대한악안면성형재건외과학회 2005 Maxillofacial Plastic Reconstructive Surgery Vol.27 No.1
It is commonly acknowledged that bone morphogenic protein (BMP-2) functions as a potential osteogenic inducer in bone formation. Recently, several papers reported that bone marrow-derived stem cell (BMSC) from human is not responsive to BMP-2 in comparison to high capacity of BMP-2 in the osteoinduction of stromal cell derived from bone marrow of rodent animals such as rat or mouse. In this study, we characterized BMSC derived from 11 years old donor for the responsiveness to rhBMP-2, dexamethasone (Dex) and 1,25-dihydroxyvitamin D (vitamin D), in order to analyze their function in the early osteogenesis. The effect of over mentioned agents was evaluated by means of assessing alkaline phosphatase (ALP) activity/staining, RT-PCR analysis and von Kossa staining. In addition, we analyzed the meaning of expressed several osteoblastic markers such as alkaline phosphatase, collagen typeI, osteopontin, bone sialoprotein and osteocalcin with relation to either differentiation or mineralization. Only in the presence of Dex, human BMSC could commit osteoblastic differentiation and matrix mineralization, and either BMP-2 or vitamin D treatment was not able to induce. But BMP-2 or Vitamin D showed potential synergy effect with Dex. ALP and bone sialoprotein were clearly expressed in response of Dex treatment compared to weak expression of osteopontin in early osteogenesis. Therefore, we expect that this study will contribute partly to elucidiating early osteogenesis mechanism in human, but variations among bone marrow donors must be considered through further study.
Integrated Biosensor for Rapid and Point-of-Care Sepsis Diagnosis
Min, Jouha,Nothing, Maria,Coble, Ben,Zheng, Hui,Park, Jongmin,Im, Hyungsoon,Weber, Georg F.,Castro, Cesar M.,Swirski, Filip K.,Weissleder, Ralph,Lee, Hakho American Chemical Society 2018 ACS NANO Vol.12 No.4
<P>Sepsis is an often fatal condition that arises when the immune response to an infection causes widespread systemic organ injury. A critical unmet need in combating sepsis is the lack of accurate early biomarkers that produce actionable results in busy clinical settings. Here, we report the development of a point-of-care platform for rapid sepsis detection. Termed IBS (integrated biosensor for sepsis), our approach leverages (i) the pathophysiological role of cytokine interleukin-3 (IL-3) in early sepsis and (ii) a hybrid magneto-electrochemical sensor for IL-3 detection. The developed platform produces test results within 1 h from native blood samples and detects IL-3 at a sensitivity of <10 pg/mL; this performance is >5-times faster and >10-times more sensitive than conventional enzyme-linked immunoadsorbent assays, the current gold standard. Using clinical samples, we show that elevated plasma IL-3 levels are associated with high organ failure rate and thus greater risk of mortality, confirming the potential of IL-3 as a sepsis diagnostic biomarker. With further system development (<I>e</I>.<I>g</I>., full automation, data security measures) and rigorous validation studies, the compact and fast IBS could be a practical clinical tool for timely diagnosis and proactive treatment of sepsis.</P> [FIG OMISSION]</BR>
Kim, Yong-Seog,Kim, Joong Kyun,Weber, Larry F. Institute of Electrical and Electronics Engineers 2017 IEEE transactions on electron devices Vol.64 No.8
<P>Exo-electron emission from MgO nanopowders sprayed on MgO thin film of alternating current plasma display panels was measured experimentally. Effects of several key parameters including panel temperature on the emission currents were measured and compared with a thermostimulated exo-electron emission model with three energy levels proposed in this paper. The results indicate that the exo-electronemission phenomenon is controlledby a thermal excitation of trapped electrons at shallow levels, similar to 0.2 similar to 0.3 eV below the conduction band edge. The MgO nanopowders increases the emission current significantly over the MgO thin film, possibly due to a higher concentration of recombination centers at deeper energy levels.</P>
Uncovering oxysterol-binding protein (OSBP) as a target of the anti-enteroviral compound TTP-8307
Albulescu, Lucian,Bigay, Joë,lle,Biswas, Bishyajit,Weber-Boyvat, Marion,Dorobantu, Cristina M.,Delang, Leen,van der Schaar, Hilde M.,Jung, Young-Sik,Neyts, Johan,Olkkonen, Vesa M.,van Kuppeveld, F Elsevier 2017 ANTIVIRAL RESEARCH Vol.140 No.-
<P>The genus Enterovirus (e.g. poliovirus, coxsackievirus, rhinovirus) of the Picornaviridae family of positive strand RNA viruses includes many important pathogens linked to a range of acute and chronic diseases for which no approved antiviral therapy is available. Targeting a step in the life cycle that is highly conserved provides an attractive strategy for developing broad-range inhibitors of enterovirus infection. A step that is currently explored as a target for the development of antivirals is the formation of replication organelles, which support replication of the viral genome. To build replication organelles, enteroviruses rewire cellular machinery and hijack lipid homeostasis pathways. For example, enteroviruses exploit the PI4KIIII beta-PI4P-OSBP pathway to direct cholesterol to replication organelles. Here, we uncover that TTP-8307, a known enterovirus replication inhibitor, acts through the PI4KIIII-PI4P-OSBP pathway by directly inhibiting OSBP activity. However, despite a shared mechanism of 1TP-8307 with established OSBP inhibitors (itraconazole and OSW-1), we identify a number of notable differences between these compounds. The antiviral activity of TTP-8307 extends to other viruses that require OSBP, namely the picornavirus encephalomyocarditis virus and the flavivirus hepatitis C virus. (C) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license</P>
Cyanophora paradoxa Genome Elucidates Origin of Photosynthesis in Algae and Plants
Price, D. C.,Chan, C. X.,Yoon, H. S.,Yang, E. C.,Qiu, H.,Weber, A. P. M.,Schwacke, R.,Gross, J.,Blouin, N. A.,Lane, C.,Reyes-Prieto, A.,Durnford, D. G.,Neilson, J. A. D.,Lang, B. F.,Burger, G.,Steiner American Association for the Advancement of Scienc 2012 Science Vol.335 No.6070