우리나라 약가제도의 변천사

배은영,Bae, Eun-Yeong 대한병원협회 2001 대한병원협회지 Vol.30 No.4

교육후기_제2기 여성낙농인 목장경영전문화 교육을 다녀와서 - 목장 주인으로서 나를 느끼게 해주었던 교육

배은영,Bae, Eun-Yeong 한국낙농육우협회 2009 낙농·육우 Vol.29 No.10

농가진의 원인 균주와 항생제 감수성에 관한 연구

배은영 ( Bae Eun Yeong ),이정덕 ( Lee Jeong Deog ),조상현 ( Jo Sang Hyeon ) 대한피부과학회 2003 대한피부과학회지 Vol.41 No.10

N/A Background : Impetigo is mostly caused by either Staphylococcus aureus(S. aureus) or group A β-hemolytic Streptococcus(Streptococcus pyogenes). As the relative preponderance of each microorganism varies greatly, so reports have been contradictory. Recently, S. aureus has been reported as the principal causative microorganism of impetigo and shown variable degrees of antimicrobial resistance. Objective : The purpose of this study is to find out the main causative microorganism and the antibiotic susceptibility of causative organism in impetigo. Method : Bacterial cultures and antimicrobial susceptibility test were done in 55 patients with impetigo. Results : S. aureus was cultured from 49 patients of 55 patients(89.1%), and the remains were coagulase negative Staphylococcus, Streptococcus, Citrobacter braakii and Klebsiella. The resistant rates of S. aureus against antibiotics were as follows ; penicillin : 98.0%, gentamicin : 69.2%, tobramycin : 65.0%, tetracycline : 62.5%, erythromycin : 57.1%, piperacillin : 36.0%, clindamycin : 17.4%, cefazolin : 9.5%, ciprofloxacin : 8.1%, imipenem : 5.0%, oxacillin : 3.9%, trimethoprim-sulfamethoxazole : 3.7%, cefuroxime : 0%, nobobiocin : 0%, teicoplanin : 0%, and vancomycin : 0%, Methicillin-resistant S. aureus(MRSA) and oxacillin-resistant S. aureus(ORSA) were cultured in three of 20 patients(15%) and one of 26 patients(3.9%), respectively The resistant rates of MRSA or ORSA against other antibiotics were as follows ; cefazolin, ciprofloxacin, erythromycin, and penicillin : 100%, tobramycin : 67%, trimethoprim-sumfamethoxazole : 25%, and teicoplanin, vancomycin, and nobobiocin : 0%. Conclusion : S. aureus was the most prominent pathogen in impetigo in this study and sensitive to cefuroxime, nobobiocin, teicoplanin, vancomycin and oxacillin, but not to penicillin, gentamicin, tobramycin, tetracycline and erythromycin. The effective antibiotics in the treatment of MRSA or ORSA were vancomycin, nobobiocin, teicoplanin and trimethoprim-sulfamethoxazole.(Korean J Dermatol 2003;41(10) : 1278~1285)

The Potential Probiotic and Functional Health Effects of Lactic Acid Bacteria Isolated from Traditional Korean Fermented Foods

Jeong-Eun Ohn(온정은),Min-Kyeong Seol(설민경),Eun-Yeong Bae(배은영),Young-Je Cho(조영제),Hee-Young Jung(정희영),Byung-Oh Kim(김병오) 한국생명과학회 2020 생명과학회지 Vol.30 No.7

본 연구에서는 한국의 전통발효식품인 식해, 열무김치, 비지에서 분리한 유산균의 프로바이오틱스로써의 사용가능성을 확인하였다. 분리된 유산균 중 pH 2.5의 산에서 60% 이상의 생존율을 나타내는 Pediococcus inopinatus BZ4, Lactobacillus plantarum SH1, Lactobacillus brevis SH14, Pediococcus pentosaceus YMT1, Leuconostoc mesenteroides YMT2는 0.3% 담즙산에서도 모두 우수한 생존율을 나타내어 이 5종을 선별하여 실험을 진행하였다. 간접적으로 유용미생물의 군집화 및 병원성 세균의 부착을 저해하는 자가 응집 및 상호 응집 실험에서 다섯 개의 유산균은 강력한 응집능을 나타내었다. 유기용매를 이용한 세포 표면 소수성 실험에서 3가지 용매에 모두 부착성을 나타내어 세포 표면의 높은 소수성을 보여주었으며 이는 간접적으로 장세포에 부착할 수 있는 세포 부착능이 우수하다는 것을 보여준다. 또한, DPPH, ABTS 라디컬 소거능 측정, 지질 과산화억제능 실험에서도 선별된 유산균의 cell-free supernatant 및 intracellular cell-free extract는 항산화 활성을 나타내었다. 마지막으로 진균인 C. albicans ATCC 10231를 제외한 4가지 병원성세균(E. coli KCTC 2571, H. pylori HPKCTC B0150, L. monocytogenes KCTC 13064, S. aureus KCTC 1916)에서 모두 항균활성이 나타남을 확인하였다. 상기 실험결과를 바탕으로, 분리된 유산균은 항산화, 항균활성을 보유하고 있는 프로바이오틱스 제제로써 활용이 가능할 것으로 기대되며 이는 기초적인 실험으로써 산업화를 위한 임상검증 등의 추가적인 연구가 필요하다고 사료된다. This study investigated the probiotic properties and physiological activities of Korean fermented foods such as sikhae, young radish kimchi, and bean-curd dregs. Among the isolated lactic acid bacteria, Pediococcus inopinatus BZ4, Lactobacillus plantarum SH1, Lactobacillus brevis SH14, Pediococcus pentosaceus YMT1, and Leuconostoc mesenteroides YMT6 demonstrated a greater than 60% survival rate at pH 2.5, along with an excellent survival rate even at 0.3% bile acid. These five bacteria showed strong floccu-lation ability in autoaggregation and coaggregation tests, indirectly clustering useful micro-organisms and inhibiting the attachment of pathogenic bacteria. In a cell surface hydrophobicity test, these bacteria showed adhesion to three solvents (ethyl acetate, chloroform, and xylene) and high hydrophobicity, thereby indicating excellent indirect cell adhesion to intestinal cells. The cell-free supernatants and intracellular extracts of the five lactic acid bacteria showed antioxidative activity in the form of 2,2-Diphenyl-1-picrylhydrazyl and 2,2"-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging ability and lipid peroxidation inhibition. Antimicrobial activities were also observed in four pathogenic bacteria, namely E. coli KCTC 2571, H. pylori HPKCTC B0150, L. monocytogenes KCTC 13064, and S. aureus KCTC 1916. These results demonstrate that these five lactic acid bacteria could be used as probiotics with antioxidant and antimicrobial properties.

Change in Lactobacillus brevis GS1022 and Pediococcus inopinatus GS316 in Gajami Sikhae Fermentation

Soo-Jeong Lim(임수정),Eun-Yeong Bae(배은영),Min-Kyeong Seol(설민경),Young-je Cho(조영제),Hee-Young Jung(정희영),Byung-Oh Kim(김병오) 한국생명과학회 2020 생명과학회지 Vol.30 No.6

유산균은 장 건강, 심장질환, 우울증, 비만 등을 예방하고 치료할 수 있다고 널리 알려져 있고, 특히 한국은 다양한 발효식품에서 유산균을 섭취할 수 있다. 하지만 국내 발효식품에서 분리된 유산균에 대한 연구는 대부분 김치에 한정되어 있기 때문에 대표적인 어류 전통 발효식품인 식해류의 유산균에 대한 연구는 미비한 실정이다. 따라서 본 연구는 한국의 전통발효식품인 가자미 식해의 발효에 관여하는 유산균의 배양일에 따른 균총의 변화를 관찰하고, 가자미 식해로부터 프로바이오틱스로 이용 가치가 있는 균을 분리하기 위해 시행되었다. 포항의 가정집에서 제조된 가자미 식해에서 유산균을 제조일로부터 50일까지 5일 간격으로 분리하고 동정하였고, 동정 결과 P. pentosaceus, P. inopinatus, Lc. mesenteroides, L. brevis, L. plantarum 5개 균주가 발효에 관여함을 알 수 있었다. 발효가 진행됨에 따라 초기 발효에는 Pediococcus sp.가, 중기 이후부터는 Lactobacillus sp.가 우세하게 발효에 관여하는 것을 확인할 수 있었다. 분리한 유산균 중 우수한 프로바이오틱 활성을 가지는 균주를 알아보기 위하여 산내성, 담즙산 내성 시험을 통해 균주를 선별한 뒤 응집능, 표면 소수성, 항균 활성, 항산화 활성 등을 평가하였다. 그 결과 본 연구의 가자미 식해 분리 균주 중 최종적으로 선택된 소화액 내성이 뛰어난 L. brevis GS1022와 응집능이 뛰어난 P. inopinatus GS316은 프로바이오틱 균주로 이용할 가치가 충분하다고 사료된다. Lactic acid bacteria are widely known to prevent and treat intestinal health conditions, heart disease, depression, and obesity. In Korea, such bacteria are commonly consumed through various fermented foods, although most are isolated from kimchi, and research on the lactic acid bacteria in fermented seafood is insufficient. This study was therefore conducted to observe changes in bacterial flora according to the culture date of lactic acid bacteria in the fermentation of traditional Korean Gajami Sikhae produced in Pohang and to isolate the bacteria of probiotic value. The bacteria were periodically isolated and identified from date of preparation to 50 days after preparation to investigate which Lactobacillus are involved in Gajami Sikhae. As fermentation progressed, it was confirmed that Pediococcus sp. and Lactobacillus sp. participate predominantly in the early and later periods of fermentation, respectively. During the entire fermentation period, 170 isolates were screened, and the following five species were found to be involved: Pediococcus pentosaceus, Pediococcus inopinatus, Leuconostoc mesenteroides, Lactobacillus brevis, and Lactobacillus plantarum. Five strains of these species were selected through acid and bile tolerance tests, and their coaggregation, autoaggregation, hydrophobicity, antibacterial, and antioxidant activities were then evaluated. As a result, it is thought that L. brevis GS1022, which has excellent digestive fluid resistance, and P. inopinatus GS316, which has excellent cohesiveness, may be useful as probiotic strains.

The Anti-oxidant and Anti-microbial Activities of Purified Syringin from Cortex Fraxini

Min-Kyeong Seol(설민경),Eun-Yeong Bae(배은영),Young-Je Cho(조영제),Soon-Ki Park(박순기),Byung-Oh Kim(김병오) 한국생명과학회 2020 생명과학회지 Vol.30 No.8

본 연구에서는 물푸레나무 껍질인 진피로부터 Syringin을 효과적으로 분리, 정제하고 항산화 및 항균 활성을 평가하여 기능성 소재로써의 가능성을 탐구하고자 하였다. Syringin은 DPPH radical 소거활성 평가에서 50 μg/ml의 적은 농도로도 BHT보다 높은 활성을 나타내었으며 ABTS radical 소거활성 평가에서는 모든 농도에서 BHT의 활성과 비슷한 정도의 활성을 나타냈다. PF 측정에서는 Syringin의 농도가 증가함에 따라 활성이 다소 높아지는 듯했으나 증가폭이 크지 않았고 모든 농도에서 1.2 PF 이상의 활성이 나타나 항산화력이 있다고 판단하였다. TBARs 활성 측정에서는 모든 농도에서 BHT의 활성보다 낮지만 농도 의존적으로 항산화력이 증가하는 것을 확인할 수 있었다. 그람양성, 그람음성, 진균에 대한 생육 억제 활성 평가에서는 L. monocytogenes KCTC 13064, S. aureus KCTC 1916, E. coli KCTC 2571, H. pylori HPKCTC B0150의 네 균주에 대한 생육억제환의 크기가 Syringin의 농도에 의존적으로 증가하였고 C. albicans ATCC 10231에 대해서는 생육억제환이 관찰되지 않았다. 위의 결과를 종합한 결과 Syringin의 수용성 물질의 항산화 활성은 지용성 물질의 항산화 활성보다 우수하고 진균을 제외한 그람양성 및 그람음성 균주에 대한 성장을 억제함으로써 항균 활성을 나타낸다고 판단하였다. 본 연구를 토대로 Syringin의 항산화 및 항세균 기작에 대한 연구와 다른 생리활성 작용, 인체 적용성에 대한 연구를 추가로 진행한다면 천연 유래의 안전한 기능성 소재로 활용 가능할 것으로 사료된다. The purpose of this study is to evaluate the anti-oxidant and anti-microbial activity of syringin isolated from Cortex Fraxini to investigate their potential for use as safe natural compounds. Purified syringin was dissolved in distilled water for each concentration and used in each experiment. Syringin showed higher 2,2-Diphenyl-1-picrylhydrazyl radical scavenging than butylated hydroxytoluene (BHT) at a concentration of 50 μg/ml. In 2,2"-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging, activity was similar to that of BHT at all concentrations. In antioxidant protection factor measurement, activity of syringin slightly increased as the concentration increased, as did the inhibitory effect of thiobarbutric acid reactive substances. In evaluating anti-microbial activity, the clear zones of Listeria monocytogenes KCTC 13064, Staphylococcus aureus KCTC 1916, Escherichia coli KCTC 2571, and Helicobacter pylori HPKCTC B0150 at a concentration of 200 μg/ml were found to be 17.8 mm, 20.45 mm, 17.05 mm, and 16.8 mm, respectively, but no clear zone was observed in the case of Candida albicans ATCC 10231. The activity against water-soluble antioxidants was therefore superior to that against lipid-soluble antioxidants. Anti-microbial activity was examined by inhibiting growth against gram-positive and -negative strains, and anti-fungal activity was not observed. Based on the results of this study, syringin has possible applications as a natural anti-oxidant and anti-microbial material.

서울 지역 내과 개원의 천식 진료 양상

이의경 ( Lee Ui Gyeong ),배은영 ( Bae Eun Yeong ),박은자 ( Park Eun Ja ),이숙향 ( Lee Sug Hyang ),오연목 ( O Yeon Mog ),인광호 ( In Gwang Ho ),유세화 ( Yu Se Hwa ) 대한결핵 및 호흡기학회 2003 Tuberculosis and Respiratory Diseases Vol.55 No.2

발효식품의 품질을 저해하는 부패효모의 발생원인과 검출 및 제어방법

정덕열(Deokyeol Jeong),박희영(Heeyoung Park),배은영(Eun-Yeong Bae),설민경(Min-Kyeong Seol),주예빈(Ye-Bin Ju),김종석(Jong-Seok Kim),장병관(Byeong-Kwan Jang),Sultanov Akhmadjon,김병오(Byung-Oh Kim),조영제(Young-Je Cho),김수린(Soo Ri 한국생물공학회 2020 KSBB Journal Vol.35 No.1

It is not commonly recognized that yeast is responsible for food spoilage as well as food fermentation. Fermented beverages and foods have several factors that inhibit bacterial growth such as high contents of organic acids, sugars, salts, and/or alcohol; however, some yeast species can tolerate these stress factors and cause food spoilage. Specifically, during wine and beer fermentation, if some contaminated spoilage yeast species grow dominantly over fermentation starter yeast, the products would have undesirable flavor. More importantly, during storage of fermented kimchi and soy sauce, some spoilage yeast species slowly grow on the surface of the products and produce gases, which limits product shelf life and exportability. In this review, spoilage yeast species commonly found in fermented foods are introduced. Next, contamination routes, detection methods, and control methods of the spoilage yeast will be described.

Polymeric Gene Carrier as a mean of targeting to Artery Wall Cells

Choi, Hey Young,Bae, Eun Yeong,Choi, Chang Yong,Jang, Mi Kyeong,Kim, Myung Yul,Nah, Jae Woon 한국공업화학회 2002 응용화학 Vol.6 No.2

Non-viral gene carriers continue to attract a great deal of interest due to advantageous safety profile. Among the non-viral gene carriers, cationic liposomes or synthetic gene carriers are efficient DNA carriers in vitro, but there in vivo applications are greatly hampered because of their low bioavailability. On the other hand, chitosan, a natural cationic polysaccharide, is a candidate non-viral vector for gene delivery because of its high positive charges and low cytotoxicity. In this work, AWBP-PEG-LMWSC was synthesized by conjugation the arterial wall binding peptide (AWBP), a specific targeting peptide, to the end of PEGylation low molecular water soluble chitosan (LMWSC) as a gene carrier to target artery wall cells. Chatacters of the synthesized AWBP-PEG-LMWSC were analyzed by measuring FT-IR, ^1H-NMR, zeta-potentiometer, atomic force microscopy (AFM), transmittance electro microscopy (TEM). And the physical and biochemical properties of AWBP-PEG-LMWSC/pDNA complexes will be also investigated.