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      • 동부(Vigna sinensis King) 자엽에서 α-amylase 활성에 미치는 식물 생장 조절의 효과

        權義貞,權五溶 충남대학교 기초과학연구소 1997 忠南科學硏究誌 Vol.24 No.1

        The effects of plant growth regulators on α-amylase activities in the embryonic axis-attached and detached cotyledons of Vigna sinensis King have been studied. In the absence of plant growth regulators, α-amylase activities in the embryonic axis-attached and detached cotyledons developing for 3 days have been generally increased, and than gradually decreased. When the 10 ^6M solution of gibberelli acid (GA_3), kinetin, indole-3-acetic acid (IAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), have been exogenously supplied in the detached cotyledons for 3 days, α-amylase activities have occurred with about 2.4-fold in GA_3, 2.8-fold in kinetin, 1.8-fold in IAA, and 3.7-fold in 2,4-D, rather than the control. In the detached cotyledons treated with the 10 ^7M solution of zeatin, and 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), α-amylase activities have been orderly increased with about 2-fold in zeatin, and 3.3-fold in 2,4,5-T. Consequently, it has been recently suggested that the 10 ^6M solution of 2,4-D besides the 10 ^7M solution of 2,4,5-T, which is a sort of synthetic auxin, has been obviously induced the development of α-amylase occurred in the embryonic axis-detached cotyledons of Vigna sinensis King.

      • KCI등재

        방사선 조사선량에 따른 백서 악하선의 caspase-3 발현양상

        권기정,최용석,황의환,이상래,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.37 No.1

        Purpose : To investigate the caspase-3 expression in the acinar and ductal cells of rat submandibular glands after the irradiation of various doses. Materials and Methods: The male Sprague-Dawley rats weighing approximately 250 gm were used for this study. The experimental group was irradiated with a single absorbed dose of 2, 5, 10, and 15 Gy on the head and neck region. The rats were sacrificed on the 1st, 3rd, 7th, 14th, 21st, and 28th day after irradiation. The specimens including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : The local destruction of the acinar and ductal cells and the karyopyknotic nuclei of the acinar cells were observed in the 2 Gy and 5 Gy irradiation groups later than in the 10 Gy and 15 Gy irradiation groups. And the expression of caspase-3 was prominent only in the ductal cells in the 2 Gy and 5 Gy irradiation groups. Conclusion : This experiment suggests that radiation-induced apoptosis in the ductal cells of rat submandibular glands was induced by a low dose radiation associated with the activation of caspase-3 and radiation-induced necrosis was induced by a high dose radiation. (Korean J Oral Maxillofac Radiol 2006; 36 : 7-15)

      • KCI등재

        발열이 있는 호중구 감소증 환자에서의 위험도 예측인자

        정중식,권운용,김규석,임용수,이중의,서길준,윤여규 대한응급의학회 2000 대한응급의학회지 Vol.11 No.3

        Background: Most febrile neutropenic patients are treated in an aggressive manner. However, identification of low-risk patients may enable clinicians to administer risk-based treatment. The object of this study is to certify the factors associated with increased risk at the time of visiting the emergency department. Methods: This is a retrospective study. We reviewed the medical records of 101 febrile neutropenic patients who had visited the emergency department of Seoul National University Hospital from January 1998 to August 1999. We assumed 22 risk prediction factors that could be assessed at admission to the emergency department and 5 factors that could be assessed during treatment course. To find independent risk-prediction factors, we analyzed these factors respectively by using multiple regression analysis. Results: Tachycardia(aOR=136.5), altered mentality(aOR=28.8), decreased renal function(aOR=20.1), and significant comorbidity(aOR=17,2) are the independent factors associated with higher mortality. Altered mentality(aOR=31.6) and decreased renal function(CCr < 75㎖/min, aOR=5.4) are those associated with a higher incidence of septic shock. Independent factors associated with persistent(more than 3 days) fever are the early(within 10 days) onset of fever after last chemotherapy(aOR=8.8) and the existence of new pulmonary infiltrates on a simple chest X-ray(aOR=4.3). Conclusion: The stability of vital signs, the change of mentality, the renal function, the existence of significant comorbidity, the existence of new pulmonary infiltrates, and the rate of neutropenia are clinically useful risk-prediction factors in febrile neutropenia at the time of visiting the emergency department.

      • KCI등재

        사망 진단서(시체 검안서) 작성의 문제점

        김규석,임용수,이중의,서길준,윤여규,어은경,염석란,정연권,이윤성 대한응급의학회 2000 대한응급의학회지 Vol.11 No.4

        Background: This study was conducted to analyze the current problems in completing death certificates and to identify the correct method for completing death certificates Methods: We reviewed 262 death certificates in three hospitals from March 1 to April 30, 2000, and 119 death certificates in one hospital from March 1 to 31, 2000. We identified major and minor errors and analyzed and compared them retrospectively. Results: A total of 381 death certificates were reviewed: 59 in Seoul National University Hospital,101 in Ewha Woman's University Hospital, and 102 in Gachon Medical College Hospital, which has no education program for completing death certificates in postgraduate training, and 119 in Samsung Medical Center which has an education program for completing death certificates. 358 certificates(94.0%) had at least one error. There were only 23 death certificates(6.0%) without an error. In 182 cases(47.8%), there was one major error. In 321 death certificates(84.3%), there were more than two errors. A comparison of Samsung Medical Center with the other hospitals showed that the number of total errors was statistically different(p=0.001). Conclusion: There were few death certificates without an error in this study. In a hospital which has postgraduate training in completing death certificates, there are fewer errors than in other hospitals which have no training course. Emergency physicians actually certify many deaths, so they must know the correct method of completing death certificates for statistics on morbidity and mortality.

      • KCI등재

        Adventitious Root Development and Ginsenoside Production in Panax ginseng, Panax quinquefolium and Panax japonicum

        Yong Eui Choi,한정연,Yong Soo Kwon 한국식물생명공학회 2006 JOURNAL OF PLANT BIOTECHNOLOGY Vol.33 No.2

        This work was carried out to establish adventitious root culture system in three Panax species (wild-grown P. ginseng, P. quinquefolium, and P. japonicum) to analyze their ginsenoside productivity. Adventitious roots were induced directly from segments of seedlings after cultured on MS (Murashige andSkoog 1962) solid medium containing 3.0 mg/l IBA. Omission of NH4NO3 from the medium greatly enhanced both the frequency of adventitious root formation and number of roots per explants in all the three Panax species. However, elongation of post-induced adventitious roots was enhanced on medium with NH4NO3. Two-step culture protocol: NH4NO3-free medium for first two weeks of culture, followed by NH4NO3 containing medium for further 4 weeks, greatly enhanced the fresh weight increase of adventitious roots in all the three ginseng species. The fresh weight of adventitious roots was high in P. quinquefolium and low in P. ginseng, followed by P. japonicum regardless of the composition of medium. Pattern and content of ginsenosides in adventitious roots differed among the three Panax species. Total ginsenoside content of adventitious roots in P. quinquefolium, P. ginseng, and P. japonicum was 8.03, 15.7 and 1.2 mg/g dry weight, respectively. Among the three speices, adventitious roots in P. quinquefolium produced highamount of ginsenosides. The pattern of ginsenoside fractions between P. ginseng and P. quinquefolium was similar but the amount of ginsenoside differed between the two. While, in P. japonicum, total ginsenoside content was very low and some ginsenosides such as ginsenoside Rb2 and Rf were not detected. Conclusively, we demonstrate that same culture condition was required for induction and elon-gation of adventitious roots of three ginseng species but growth of adventitious roots and their ginsenoside production were different among them. This work was carried out to establish adventitious root culture system in three Panax species (wild-grown P. ginseng, P. quinquefolium, and P. japonicum) to analyze their ginsenoside productivity. Adventitious roots were induced directly from segments of seedlings after cultured on MS (Murashige andSkoog 1962) solid medium containing 3.0 mg/l IBA. Omission of NH4NO3 from the medium greatly enhanced both the frequency of adventitious root formation and number of roots per explants in all the three Panax species. However, elongation of post-induced adventitious roots was enhanced on medium with NH4NO3. Two-step culture protocol: NH4NO3-free medium for first two weeks of culture, followed by NH4NO3 containing medium for further 4 weeks, greatly enhanced the fresh weight increase of adventitious roots in all the three ginseng species. The fresh weight of adventitious roots was high in P. quinquefolium and low in P. ginseng, followed by P. japonicum regardless of the composition of medium. Pattern and content of ginsenosides in adventitious roots differed among the three Panax species. Total ginsenoside content of adventitious roots in P. quinquefolium, P. ginseng, and P. japonicum was 8.03, 15.7 and 1.2 mg/g dry weight, respectively. Among the three speices, adventitious roots in P. quinquefolium produced highamount of ginsenosides. The pattern of ginsenoside fractions between P. ginseng and P. quinquefolium was similar but the amount of ginsenoside differed between the two. While, in P. japonicum, total ginsenoside content was very low and some ginsenosides such as ginsenoside Rb2 and Rf were not detected. Conclusively, we demonstrate that same culture condition was required for induction and elon-gation of adventitious roots of three ginseng species but growth of adventitious roots and their ginsenoside production were different among them.

      • SCIESCOPUSKCI등재

        Experimental Study on the Mean Flow Characteristics of Forward-Curved Centrifugal Fans

        Kwon, Eui-Yong,Cho, Nam-Hyo The Korean Society of Mechanical Engineers 2001 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.15 No.12

        Measurements have been made in an automotive HVAC b1ower for two different centrifugal fans. This work is directed at improving the performance of a conventional forward-curved centrifugal fan for a given small blower casing. Mean velocities and pressure have been measured using a miniature five-hole probe and a pressure scanning unit connected to an online data acquisition system. First, we obtained the fan performance versus flow rates showing a significant attenuation of unstable nature achieved with the new fan rotor in the surging operation range. Second, aerodynamic characterizations were carried out by investigating the velocity and pressure fields in the casing flow passage for different fan operating conditions. The measurements stowed that performance coefficients are strongly influenced by flow characteristics at the throat region. The main flow features ware common in both fans, but improved performance is achieved with tole new fan rotor, particularly in lower flow rate legions. Based on the measured results, design improvements were carried out in an acceptable operation range, which gave considerable insight into what features of flow behavior ware most important.

      • KCI등재SCOPUS
      • 자동차 에어컨용 전곡형 원심 송풍기의 공력성능 분석

        권의용(Eui-Yong Kwon),조남효(Nam-Hyo Cho) 한국유체기계학회 2000 유체기계 연구개발 발표회 논문집 Vol.- No.-

        Aerodynamic optimization of an automotive air-conditioning blower is a hard task because of the highly complex flow phenomena related to three-dimensional flow separations and the unsteady nature caused by the interaction between primary and secondary air flows throughout the fan. In this paper, an aerodynamic study on a forward-curved centrifugal fan has been carried out. Firstly we obtained the fan performance curves versus flow rates showing its unstable nature in the surging operation range. Secondly aerodynamic characterizations were carried out by investigating the velocity and pressure fields in the casing flow passage using a 5-hole pitot probe, at different operating conditions. Surface flow pattern near the cut-off area exhibits similar flow behavior above the best efficiency operating point, although the pressure level increases substantially with the increase of flow rate. Vorticity in the casing passage flow occurs in all flow rates, downstream from the r-Z plane θ=120 deg., where the position of its core changes with the circumferential location. Although complex, the general flow behavior were common, giving insight in its main aerodynamic features.

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