COVID-19 상황에서 온라인 비대면 수업에 대한 초등교사의 인식 - 과학교과를 중심으로 -

강유진 ( Kang¸ Eugene ),정도준 ( Jeong¸ Dojun ),박지훈 ( Park¸ Jihun ),김지나 ( Kim¸ Jina ),박종석 ( Park¸ Jongseok ),남정희 ( Nam¸ Jeonghee ) 한국초등과학교육학회 2021 초등과학교육 Vol.40 No.4

COVID-19 팬데믹의 위기는 전 세계적으로 교육 시스템에 큰 변화를 초래하였다. 우리나라에서도 COVID-19로 인한 사회적 거리두기 정책에 따라 갑작스럽게 비대면 수업으로 전환되었다. 준비 없는 비대면 수업으로 인해 초등학교 현장에서 발생하는 문제들로 볼 때 이와 관련된 연구가 필요한 상황이다. 본 연구는 초등교사가 인식하는 비대면 수업에서 과학교육의 목표와 방법, 과학탐구활동을 포함한 비대면 수업에서 발생한 문제와 초등교사의 대응, 비대면 수업 경험 후 비대면수업의 장단점과 교사의 역할에 대한 초등교사의 인식을 알아보고자 한다. 온라인 콘텐츠 활용 수업과 온라인 실시간 쌍방향 수업을 포함하는 온라인 비대면 수업을 시행한 초등교사를 대상으로 설문조사(153명)와 포커스 인터뷰(9명)를 실시하였다. 연구 결과에서 3가지 결론과 제안점을 도출하였다. 첫째, 초등교사들은 온라인 비대면 수업에서 비대면 방식에 따라 일방향 강의와 쌍방향 강의를 하였다. 온라인 비대면 수업에서 조사나 토론 등 강의 외의 수업방법을 활용하기 위해서는 교사들의 디지털 리터러시를 높일 필요가 있다. 둘째, 초등교사들은 비대면 수업에서 과학탐구활동의 어려움으로 현장 피드백의 어려움, 준비물 확보의 어려움, 안전 확보의 어려움을 인식하였다. 개인 실험을 위한 실험 꾸러미와 실험 영상을 제공하고 온라인에서 교사-학생, 학생-학생 간 협동적 논의와 피드백을 통해서 어려움을 극복할 수 있다. 셋째, 초등교사들은 비대면 수업의 장점으로 IT 기기를 사용하는 다양한 형태의 수업이 가능하고 개별화된 학습이 가능하다고 인식하였다. 단점으로는 탐구활동이나 협동학습과 같은 대면 기반 수업이 어렵고, 학생의 이해 정도를 알 수 없어서 피드백이 어려우며, 학생간의 상호작용이 어렵고, 학습 격차가 벌어진다고 인식하였다. The crisis of the COVID-19 pandemic has caused significant changes in education systems worldwide, including in Korea. Due to COVID-19’s social distancing policies, the education system was suddenly switched to distance learning, resulting in many problems in primary schools without preparation. The purpose of this study was to investigate a teacher’s awareness of science education techniques, responses to issues in science classes, including inquiry activities, advantages and disadvantages, and roles after experiencing distance learning. Survey and focus interviews were conducted for primary school teachers who had previously participated in distance learning, such as online content classes and real-time interactive classes. The study findings showed three conclusions: 1) Primary school teachers conducted one-way and interactive lectures in online classes. It is vital to improve a teacher’s digital literacy to improve other teaching methods such as investigation and discussion in online classes. 2) Primary school teachers acknowledged the challenges of field feedback, inquiry item preparations, and safety in inquiry activities of science classes, by providing individual experimental packages and videos and using online discussion and feedback among teacher-student and student-student interactions. 3) Primary school teachers recognized that various types of classes using IT devices and individualized learning were possible as advantages of distance learning. As for disadvantages, it was acknowledged that inquiry activities, cooperative learning, immediate feedback, and interaction among students were challenging. Furthermore, learning gaps were wider in distance learning.

The effect of rhinovirus on airway inflammation in a murine asthma model

Kim, Eugene,Lee, Huisu,Kim, Hyun Sook,Won, Sulmui,Lee, Eu Kyoung,Kim, Hwan Soo,Bang, Kyongwon,Chun, Yoon Hong,Yoon, Jong-Seo,Kim, Hyun Hee,Kim, Jin Tack,Lee, Joon Sung The Korean Pediatric Society 2013 Clinical and Experimental Pediatrics (CEP) Vol.56 No.11

Purpose: The aim of the present study was to investigate the differences in lower airway inflammatory immune responses, including cellular responses and responses in terms of inflammatory mediators in bronchoalveolar lavage fluid (BALF) and the airway, to rhinovirus (RV) infection on asthma exacerbation by comparing a control and a murine asthma model, with or without RV infection. Methods: BALB/c mice were intraperitoneally injected with a crude extract of Dermatophagoides farinae (Df ) or phosphate buffered saline (PBS) and were subsequently intranasally treated with a crude extract of Df or PBS. Airway responsiveness and cell infiltration, differential cell counts in BALF, and cytokine and chemokine concentrations in BALF were measured 24 hours after intranasal RV1B infection. Results: RV infection increased the enhanced pause (Penh) in both the Df sensitized and challenged mice (Df mice) and PBS-treated mice (PBS mice) (P<0.05). Airway eosinophil infiltration increased in Df mice after RV infection (P<0.05). The levels of interleukin (IL) 13, tumor necrosis factor alpha, and regulated on activation, normal T cells expressed and secreted (RANTES) increased in response to RV infection in Df mice, but not in PBS mice (P<0.05). The level of IL-10 significantly decreased following RV infection in Df mice (P<0.05). Conclusion: Our findings suggest that the augmented induction of proinflammatory cytokines, Th2 cytokines, and chemokines that mediate an eosinophil response and the decreased induction of regulatory cytokines after RV infection may be important manifestations leading to airway inflammation with eosinophil infiltration and changes in airway responsiveness in the asthma model.

Cardiovascular Risk Is Elevated in Lean Subjects with Nonalcoholic Fatty Liver Disease

Kim Yuna,Han Eugene,Lee Jae Seung,Lee Hye Won,Kim Beom Kyung,Kim Mi Kyung,Kim Hye Soon,Park Jun Yong,Kim Do Young,Ahn Sang Hoon,Lee Byung-Wan,Kang Eun Seok,Cha Bong-Soo,Lee Yong-ho,Kim Seung Up 거트앤리버 소화기연관학회협의회 2022 Gut and Liver Vol.16 No.2

Background/Aims: Nonalcoholic fatty liver disease (NAFLD) and obesity are independently associated with an increased risk for atherosclerotic cardiovascular disease (ASCVD), the leading cause of mortality in patients with NAFLD. Many NAFLD patients are lean, but their ASCVD risk compared to obese subjects with NAFLD is unclear. Methods: Data from the 2008 to 2011 Korea National Health and Nutrition Examination Surveys database were analyzed (n=4,786). NAFLD was defined as a comprehensive NAFLD score ≥40 or a liver fat score ≥–0.640. ASCVD risk was evaluated using the American College of Cardiology/ American Heart Association guidelines. Results: The frequency of subjects without NAFLD, with obese NAFLD, and with lean NAFLD was 62.4% (n=2,987), 26.6% (n=1,274), and 11.0% (n=525), respectively. Subjects with lean NAFLD had a significantly higher ASCVD score and prevalence of a high ASCVD risk (mean 15.6±14.0, 51.6%) than those with obese NAFLD and without NAFLD (mean 11.2±11.4, 39.8%; mean 7.9±10.9, 25.5%; all p<0.001). Subjects with lean NAFLD and significant liver fibrosis showed a significantly higher odds ratio for a high risk for ASCVD than those with obese NAFLD with or without significant liver fibrosis (odds ratio, 2.60 vs 1.93; p=0.023). Conclusions: Subjects with lean NAFLD had a significantly higher ASCVD score and prevalence of high risk for ASCVD than those with obese NAFLD. Similarly, lean subjects with significant liver fibrosis had a higher probability of ASCVD than obese subjects in the subpopulation with NAFLD.

A library of TAL effector nucleases spanning the human genome

Kim, Yongsub,Kweon, Jiyeon,Kim, Annie,Chon, Jae Kyung,Yoo, Ji Yeon,Kim, Hye Joo,Kim, Sojung,Lee, Choongil,Jeong, Euihwan,Chung, Eugene,Kim, Doyoung,Lee, Mi Seon,Go, Eun Mi,Song, Hye Jung,Kim, Hwangbeo Nature Publishing Group, a division of Macmillan P 2013 Nature biotechnology Vol.31 No.3

Transcription activator–like (TAL) effector nucleases (TALENs) can be readily engineered to bind specific genomic loci, enabling the introduction of precise genetic modifications such as gene knockouts and additions. Here we present a genome-scale collection of TALENs for efficient and scalable gene targeting in human cells. We chose target sites that did not have highly similar sequences elsewhere in the genome to avoid off-target mutations and assembled TALEN plasmids for 18,740 protein-coding genes using a high-throughput Golden-Gate cloning system. A pilot test involving 124 genes showed that all TALENs were active and disrupted their target genes at high frequencies, although two of these TALENs became active only after their target sites were partially demethylated using an inhibitor of DNA methyltransferase. We used our TALEN library to generate single- and double-gene-knockout cells in which NF-κB signaling pathways were disrupted. Compared with cells treated with short interfering RNAs, these cells showed unambiguous suppression of signal transduction.

The novel cytokine p43 induces IL-12 production in macrophages via NF-kappaB activation, leading to enhanced IFN-gamma production in CD4+ T cells.

Kim, Eugene,Kim, Seung Hyun,Kim, Sunghoon,Kim, Tae Sung Williams Wilkins 2006 JOURNAL OF IMMUNOLOGY Vol.176 No.1

<P>Recently, we determined that p43, an auxiliary factor of mammalian multiaminoacyl-tRNA synthetases, is secreted, and functions as a novel pleiotropic cytokine. In this study, we have attempted to characterize the effects of p43 on the generation of IL-12 in mouse macrophages. p43 was determined to induce significant IL-12 production from mouse macrophages in a dose-dependent manner. The stimulatory effect of p43 on the activation of IL-12p40 promoter was mapped to a region harboring an NF-kappaB binding site. The nuclear extracts from the p43-stimulated macrophages exhibited profound NF-kappaB DNA-binding activity, as determined by the EMSA. In addition, the p43-stimulated IL-12 induction and NF-kappaB DNA-binding activity were significantly suppressed by caffeic acid phenethyl ester and BAY11-7082, both inhibitors of NF-kappaB activation, indicating that p43 induced the production of IL-12 in macrophages mainly via the activation of NF-kappaB. Importantly, p43 increased the level of IFN-gamma production in the Ag-primed lymph node cells, but had no effect on IL-4 levels. The addition of a neutralizing anti-IL-12p40 mAb to the cell cultures resulted in a decrease of the production of p43-enhanced IFN-gamma by the keyhole limpet hemocyanin-primed lymph node cells. Furthermore, coincubation with p43-pretreated macrophages enhanced the production of IFN-gamma by the keyhole limpet hemocyanin-primed CD4+ T cells, thereby indicating that p43 may enhance IFN-gamma expression in CD4+ T cells via the induction of IL-12 production in macrophages. These results indicate that p43 may play an essential role in the development of the Th1 immune responses associated with cancer immunotherapy and protective immunity against intracellular pathogens.</P>

AIMP1/p43 protein induces the maturation of bone marrow-derived dendritic cells with T helper type 1-polarizing ability.

Kim, Eugene,Kim, Seung Hyun,Kim, Sunghoon,Cho, Daeho,Kim, Tae Sung Williams Wilkins 2008 JOURNAL OF IMMUNOLOGY Vol.180 No.5

<P>AIMP1 (ARS-interacting multifunctional protein 1), previously known as p43, was initially identified as a factor associated with a macromolecular tRNA synthetase complex. Recently, we demonstrated that AIMP1 is also secreted and acts as a novel pleiotropic cytokine. In this study, we investigated whether AIMP1 induces the activation and maturation of murine bone marrow-derived dendritic cells (DCs). AIMP1-treated DCs exhibited up-regulated expression of cell-surface molecules, including CD40, CD86, and MHC class II. Additionally, microarray analysis and RT-PCR determinations indicated that the expression of known DC maturation genes also increased significantly following treatment with AIMP1. Treatment of DCs with AIMP1 resulted in a significant increase in IL-12 production and Ag-presenting capability, and it also stimulated the proliferation of allogeneic T cells. Importantly, AIMP1-treated DCs induced activation of Ag-specific Th type 1 (Th1) cells in vitro and in vivo. AIMP1-stimulated DCs significantly enhanced the IFN-gamma production of cocultured CD4+ T cells. Immunization of mice with keyhole limpet hemocyanin-pulsed AIMP1 DCs efficiently led to Ag-specific Th1 cell responses, as determined by flow cytometry and ELISA. The addition of a neutralizing anti-IL-12 mAb to the cell cultures that had been treated with AIMP1 resulted in the decreased production of IFN-gamma, thereby indicating that AIMP1-stimulated DCs may enhance the Th1 response through increased production of IL-12 by APCs. Taken together, these results indicate that AIMP1 protein induces the maturation and activation of DCs, which skew the immune response toward a Th1 response.</P>

Solitary bone and brain metastasis in a patient with papillary thyroid carcinoma mimicking cavernous angioma

( Eugene Han ),( Ye Yeon Lee ),( El Kim ),( Sun Young Kwon ),( Sang Pyo Kim ),( Hye Soon Kim ),( Mi Kyung Kim ) 대한내과학회 2016 The Korean Journal of Internal Medicine Vol.31 No.5

PARENTAL PERCEPTION OF EDUCATION : A CROSS-NATIONAL SURVEY

KIM, EUGENE C.,PARK, JOON HEE 이화여자대학교 동서교육연구소 1980 East west education Vol.1 No.1

A Method of Expanding Operating Frequency Band in a Reverberating TEM Cell by Using a Wire Septum

KIM, Hye-Kwang,KIM, Jung-Hoon,RHEE, Eugene,YANG, Sung-Il The Institute of Electronics, Information and Comm 2010 IEICE TRANSACTIONS ON COMMUNICATIONS - Vol.93 No.11

<P>This paper presents a method of expanding the operating frequency band of a Reverberating TEM Cell (RTC) for electromagnetic compatibility (EMC) testing. To expand the operating frequency band of an RTC, this paper places a wire septum inside the cell instead of a solid septum. The maximum usable frequency (MUF) for TEM cell operation and the lowest usable frequency (LUF) for reverberating chamber operation with the wire septum are studied and compared with a conventional solid septum. The E field strengths inside the RTC are measured and evaluated. The measurement results show that the RTC with the wire septum have similar MUF to the RTC with a solid septum at TEM mode, but have much lower LUF at a reverberating mode, which proves that the operating frequency band of the RTC can be expanded by using the wire septum.</P>

In vivo 3D Kinematics of Axis of Rotation in Malunited Monteggia Fracture Dislocation

Eugene Kim,Se-Jin Park,Haw-Jae Jeong,Jin Whan Ahn,Hun-Kyu Shin,Jai Hyung Park,Mi Yeon Lee,Murase Tsuyoshi,Ikemototo Sumika,Sugamoto Kazuomi,Young-Min Choi 대한견주관절의학회 2014 대한견주관절학회지 Vol.17 No.1

Background: Normal elbow joint kinematics has been widely studied in cadaver, whilst in vivo study, especially of the forearm, is rare. Our study analyses, in vivo, the kinematics of normal forearm and of malunited forearm using a three-dimensional computerized simulation system. Methods: We examined 8 patients with malunited Monteggia fracture and 4 controls with normal elbow joint. The ulna and radius were reconstructed from CT data placing the forearm in three different positions; full pronation, neutral, and full supination using computer bone models. We analyzed the axis of rotation 3-dimentionally based on the axes during forearm rotation from full pronation to full supination. Results: Axis of rotation of normal forearm was pitch line, with a mean range of 2 mm, from full pronation to full supination, connecting the radial head center proximally and ulnar fovea distally. In normal forearm, the mean range was 1.32 mm at the proximal radioulnar joint and 1.51 mm at the distal radioulnar joint. However in Monteggia fracture patients, this range changed to 7.65 mm at proximal and 4.99 mm at distal radoulnar joint. Conclusions: During forearm rotation, the axis of rotation was constant in normal elbow joint but unstable in malunited Monteggia fracture patients as seen with radial head instability. Therefore, consideration should be given not only to correcting deformity but also to restoring AOR by 3D kinematics analysis before surgical treatment of such fractures.