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Eojin Jo,Mi-Hwa Choi,Hwa-Sook Kim,Soon-Nang Park,Yun Kyong Lim,Christina K. Kang,Joong-Ki Kook KOREAN ACADAMY OF ORAL BIOLOGY 2014 International Journal of Oral Biology Vol.39 No.2
The aim of this study was to investigate antimicrobialeffect of oleanolic acid (OA), ursolic acid (UA), andsophoraflavanone G against Enterococcus faecalis andPropionibacterium acnes, which are the major causativebacteria of endodontic infections. The antimicrobial activitywas evaluated by the minimal inhibitory concentration(MIC). The data showed that the OA, UA, andsophoraflavanone G had antimicrobial effect on all thestrains use in the study with 16-64 μg/ml, 8-64 μg/ml, and 1-8μg/ml of MIC values, respectively. These results indicate thatOA, UA, and sophoraflavanone G could be useful in thedevelopment of antiseptic solution for washing the root canal in endodontic treatments.
Eojin Jo,Soon-Nang Park,Joong-Ki Kook 대한구강생물학회 2016 International Journal of Oral Biology Vol.41 No.4
Aggregatibacter actinomycetemcomitans bacteria grown on the tryptic soy-serum-bacitracin-vancomycin (TSBV) medium, an A. actinomycetemcomitans selective medium. A total of 82 unidentified bacterial isolates from the oral cavities of a Korean population were kindly provide by the Korean Collection for Oral Microbiology. All the clinical isolates were grown on TSBV medium and bacterial DNA purified from each isolate was subjected to PCR with universal primers specific for bacterial 16S rRNA genes (16S rDNAs) sequence. The each bacterial 16S rDNA was amplified by PCR and the nucleotide sequences of it was determined by the dideoxynucleotide chain termination method. They were identified by 16S rDNA sequence comparison method at the specie-level. The data showed that Neisseria spp. (42 strains), Fusobacterium spp. (10 strains), Capnocytophaga spp. (8 strains), Propionibacterium acnes (5 strains), Aggregatibacter aprophilus (4 strains), Campylobacter spp. (5 strains), Veillonella dispar (3 strains), Streptococcus sp. (1 strain), Haemophilus parainfluenzae (1 strain), Leptotrichia wadei (1 strain), Morococcus sp./ Neisseria sp. (1 strain), and Staphylococcus sp. (1 strain) were identified. These results could be used to develop a new A. actinomycetemcomitans-selective medium which is more effective than the TSBV medium in future studies.
Capnocytophaga endodontalis sp. nov., Isolated from a Human Refractory Periapical Abscess
Jo, Eojin,Park, Soon-Nang,Lim, Yun Kyong,Paek, Jayoung,Shin, Yeseul,Kim, Hongik,Kim, Si Hyun,Shin, Jeong Hwan,Chang, Young-Hyo,Kook, Joong-Ki Springer-Verlag 2018 Current microbiology Vol.75 No.4
<P>A novel Gram-negative, capnophilic, fusiform bacterium, designated strain ChDC OS43(T), was isolated from a human refractory periapical abscess in the left mandibular second molar and was characterized by polyphasic taxonomic analysis. The 16S rRNA gene sequence revealed that the strain belongs to the genus Capnocytophaga, as it showed sequence similarities to Capnocytophaga ochracea ATCC 27872(T) (96.30%) and C. sputigena ATCC 33612(T) (96.16%). The prevalent fatty acids of strain ChDC OS43(T) were isoC(15:0) (57.54%), C-16:0 (5.93%), C-16:0 3OH (5.72%), and C-18:1 cis 9 (4.41%). The complete genome of strain ChDC OS43(T) was 3,412,686 bp, and the G+C content was 38.2 mol%. The average nucleotide identity (ANI) value between strain ChDC OS43(T) and C. ochracea ATCC 27872(T) or C. sputigena ATCC 33612(T) was > 92.01%. The genome-to-genome distance (GGD) value between strain ChDC OS43(T) and C. ochracea ATCC 27872(T) or C. sputigena ATCC 33612(T) was 32.0 and 45.7%, respectively. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC OS43(T) (= KCOM 1579(T) = KCTC 5562(T) = KCCM 42841(T) = JCM 32133(T)) should be classified as the type strain of a novel species of genus Capnocytophaga, for which the name Capnocytophaga endodontalis sp. nov. is proposed.</P>
Antimicrobial Activity of Mulberry Leaf against Mutans Streptococci and Periodontopathogens
Soon-Nang Park,Yun Kyong Lim,Eugene Cho,Eojin Jo,Pyoung-Sim Park,Joong-Ki Kook KOREAN ACADAMY OF ORAL BIOLOGY 2014 International Journal of Oral Biology Vol.39 No.4
This study investigated the antimicrobial activity of methanol extract of mulberry leaf against 16 strains of mutans streptococci and four species of periodontopathogens: Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of the crude extracts or silica gel chromatography fractions of methanol-extracted mulberry leaf were evaluated by determining minimal inhibitory concentrations using an established microdilution method. The cytotoxicity of the extracts of mulberry leaf on KB cells was tested by the methyl thiazolyl tetrazolium assay. Chromatography fraction 12 displayed the most potent antimicrobial activity against all 16 strains of mutans streptococci, P. gingivalis, and P. intermedia. No KB cell cytotoxicity was evident up to 128 μg/ml of fraction 12. The methanol extract had no antimicrobial activity against F. nucleatum and A. actinomycetemcomitans. These results suggest chromatography fraction 12 methanol extract of mulberry leaf could be useful in the development of oral hygiene products, such as dentifrice and oral hygiene solution, for the prevention of dental caries.
Peptoniphilus mikwangii sp. nov., Isolated from a Clinical Specimen of Human Origin
Cho, Eugene,Park, Soon-Nang,Shin, Yeseul,Lim, Yun Kyong,Paek, Jayoung,Kim, Hak Kyun,Hwang, Cheol Ho,Jo, Eojin,Jin, Dongchun,Chang, Young-Hyo,Kook, Joong-Ki Springer-Verlag 2015 Current microbiology Vol.70 No.2
<P>A novel Gram-positive, anaerobic, and coccus-shaped bacterium, designated as strain ChDC B134(T), was isolated from a human postoperative infectious lesion in the right maxilla between the lateral incisor and canine and was characterized by polyphasic taxonomic analysis. 16S rRNA gene sequence analysis revealed that the strain ChDC B134(T) belonged to the genus Peptoniphilus, as it showed sequence similarities to Peptoniphilus indolicus KCTC 15023(T) (94.0 %) and Peptoniphilus asaccharolyticus KCTC 3321(T) (93.8 %). The prevalent fatty acids of of strain ChDC B134(T) were C16:0 (20.3 %), C18:1 cis 9 (34.3 %), and C18:0 (13.2 %). The DNA G+C content was 30.9 mol%. The cell wall diamino acid was D-ornithine, which is a property shared by other reference type strains of the genus Peptoniphilus. Based on the results of phenotypic, chemotaxonomic, and phylogenetic analysis, strain ChDC B134(T) (=KCOM 1628(T) = KCTC 15227(T) = JCM 30223(T)) should be classified as the type strain of a novel species of genus Peptoniphilus, for which the name Peptoniphilus mikwangii sp. nov. is proposed.</P>