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El Ezzi, Asmahan Ali,Zaidan, Wissam Rateeb,El-Saidi, Mohammed Ahmed,Al-Ahmadieh, Nabil,Mortenson, Jeffrey Benjamin,Kuddus, Ruhul Haque Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.3
Background: The aim of the study was to investigate any associations between benign prostate hyperplasia (BPH) and single nucleotide polymorphisms (SNPs) in the VDR gene (FokI, BsmI, ApaI and Taq${\alpha}$I loci) and the CYP17 gene (MspA1I locus), as well as TA repeat polymorphism in SRD5A2 gene among Lebanese men. Materials and Methods: DNA extracted from blood of 68 subjects with confirmed BPH and 79 age-matched controls was subjected to PCR/PCR-restriction fragment length polymorphism analysis. The odds ra=tio (OR) of having a genotype and the relative risk (RR) of developing BPH for having the genotype were calculated and the alleles were designated risk-bearing or protective. Results: Our data indicated that the A and B alleles of the VDR ApaI and BsmI SNPs were highly associated with increased risk of BPH (p=0.0168 and 0.0002, respectively). Moreover, 63% of the controls compared to 43% of the subjects with BPH were homozygous for none of the risk-bearing alleles (p=0.0123) whereas 60% of the controls and 28% of the subjects with BPH were homozygous for two or more protective alleles (p<0.0001). Conclusions: For the first time, our study demonstrated that ApaI and BsmI of the VDR gene are associated with risk of BPH among Lebanese men. Our study also indicated that overall polymorphism profile of all the genes involved in prostate physiology could be a better predictor of BPH risk.
El-Said I. El-Shafey,Haider A. J. Al-Lawati,Wafa S. H. Al-Saidi 한국탄소학회 2016 Carbon Letters Vol.19 No.-
Date palm leaflets were used as a precursor to prepare dehydrated carbon (DC) via phosphoric acid treatment at 150°C. DC, acidified with H3PO4, was converted to activated carbon (AC) at 500°C under a nitrogen atmosphere. DC shows very low surface area (6.1 m2/g) while AC possesses very high surface area (829 m2/g). The removal of lisinopril (LIS) and chlorpheniramine (CP) from an aqueous solution was tested at different pH, contact time, concentration, and temperature on both carbons. The optimal initial pH for LIS removal was 4.0 and 5.0 for DC and AC, respectively. However, for CP, initial pH 9.0 showed maximum adsorption on both carbons. Adsorption kinetics showed faster removal on AC than DC with adsorption data closely following the pseudo second order kinetic model. Adsorption increases with temperature (25°C–45°C) and activation energy (Ea) is in a range of 19–25 kJ mol/L. Equilibrium studies show higher adsorption on AC than DC. Thermodynamic parameters show that drug removal is endothermic and spontaneous with physical adsorption dominating the adsorption process. Column adsorption data show good fitting to the Thomas model. Despite its very low surface area, DC shows ~70% of AC drug adsorption capacity in addition of being inexpensive and easily prepared.
Zineb Nejjar El Ansari,Brahim El Bouzdoudi,Tomader Errabii,Rabah Saidi,Mohammed L’bachir El Kbiach 한국식물생명공학회 2021 JOURNAL OF PLANT BIOTECHNOLOGY Vol.48 No.1
The present work aims to study the induction of somatic embryogenesis in cork oak (Quercus suber L.) from immature zygotic embryos and young apical leaves obtained from 2-month-old seedlings through acorn germination on sterilized peat. The immature zygotic embryos were grown for 1 month on the mineral solution of MS in the presence of 4.52 μM 2,4-D and 30 g/L sucrose. They were then transferred to the same mineral solution with no added growth regulators. In the third subculture, yellow somatic embryos, characterized by two voluminous cotyledons, were differentiated from the radicle of the immature zygotic embryos. The induction of somatic embryogenesis in young leaves required a series of transfers on different culture media containing 30 g/L sucrose and 100 mg/L myo-inositol. Secondary or recurrent somatic embryogenesis occurred within the immature somatic embryo radicles after 1 month of culture on growth regulator-free medium containing WPM macronutrients, MS micronutrients, and vitamins.
Hayat Saidi,Abdelouahed Tounsi,Mohammed Sid Ahmed Houari,El Abbas Adda Bedia 국제구조공학회 2013 Steel and Composite Structures, An International J Vol.15 No.2
This paper presents an analytical solution to the thermomechanical bending analysis of functionally graded sandwich plates by using a new hyperbolic shear deformation theory in which the stretching effect is included. The modulus of elasticity of plates is assumed to vary according to a power law distribution in terms of the volume fractions of the constituents. The core layer is still homogeneous and made of an isotropic ceramic material. The effects of functionally graded material (FGM) layer thickness, volume fraction index, layer thickness ratio, thickness ratio and aspect ratio on the deflections and stresses of functionally graded sandwich plates are investigated.
M.S. El-Shahawi,A.A. Al-Sibaai,A.S. Bashammakh,H. Alwael,H.M. Al-Saidi 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.28 No.-
The sorption profile of trace concentrations of bismuth(III) species from aqueous KI–H2SO4 media ontothe ion pairing procaine hydrochloride (PQ+.Cl ) based polyurethane foam (PUFs) was studied. A dual–mode of bismuth(III) sorption as a ternary complex ion associate [PQ+.BiI4] involving absorption due toa ‘‘weak base anion exchanger’’ and an added component for ‘‘surface adsorption’’ seems the proposedmechanism for bismuth(III) retention. The capacity of bismuth(III) sorption was found equal40.05 1.10 mg g 1. Complete separation of spiked bismuth(III) at various concentrations (5–15 mg mL 1)1) from water onto the proposed sorbent packed columns at 5 mL min 1 flow rate was successfully achieved. The retained bismuth(III) species were recovered quantitatively from the sorbent packed columns(98.4 2.4%, n = 5) with HNO3 (1.0 mol L 1). Bismuth(V) after reduction to Bi(III) was also preconcentrated,recovered from the sorbent packed column and subsequently analyzed by inductively coupled plasma–optical emission spectrometry (ICP–OES). The height equivalent to the theoretical plates (HETP), number ofplates (N), critical and breakthrough capacities of sorbent packed column towards bismuth(III) retentionwere evaluated. Based on these results, a simple and sensitive PQ+.Cl –PUF packed column was developed fordetermination and speciation of trace concentrations of bismuth(III & V) species in water by ICP–OES. Thelimits of detection (LOD) and quantification (LOQ) of Bi(III) were found 0.09 and 0.30 mg L 1, respectively. The relative standard deviation (RSD) for 5 replicates of 50 ng L 1 Bi at 2.0 mL min 1 flow was 3.7%. Theproposed column was validated and applied for the preconcentration, separation and subsequentdetermination of analyte in Environmental water reference material (TMDW) and water samples. Theresults were found to be in good agreement with the CRM at the 95% confidence level.
M.S. El-Shahawi,A. Hamza,A.A. Al-Sibaai,A.S. Bashammakh,H.M. Al-Saidi 한국공업화학회 2013 Journal of Industrial and Engineering Chemistry Vol.19 No.2
A simple new micelle mediated preconcentration method was developed for analysis of sunset yellow (SY) prior to its spectrophotometric determination. The method was based upon cloud point extraction of the ion associate of SY and trioctylamine (TOA) in HCl–Triton X-100. In the surfactant phase the SY species react with TOA yielding hydrophobic ion associate of SY-+TOA+. The distribution coefficient of SY between surfactant-rich phase and aqueous phase was approximately 104. Validation was tested by comparing the results with standard HPLC. Isotherm and thermodynamic parameters, chemical equilibrium, extraction constants and stiochiometry of the associate were assigned.
Nasreddine Hfayedh,Wassila Saidi,Mohamed Haouari,C ecile Autret-Lambert,Sylvain Roger,Adel Megriche,Mohamed El Maaoui 한국물리학회 2016 Current Applied Physics Vol.16 No.10
Titanate nanotubes were synthesized via hydrothermal treatment of rutile TiO2. The reaction conditions were: particles sizes of TiO2 having diameter between 0.05 and 0.5 mm, 10 M NaOH solution and a temperature of 150 C. The effect of reaction time on the morphology and the crystalline structure were investigated by X-ray diffraction (XRD), Raman spectroscopy and High Resolution Transmission Electron Microscopy (HRTEM). Results showed that the reaction products are composed of nanotubes having 3, 4 and 5 layers. All the three forms coexist together in each sample with different fractions. The number of layers of the major form increases from 3 to 5 layers when reaction time increases from 12 to 120 h. At the same time, internal and external diameters vary from 3 nm to 5 nm and from 7 nm to 11 nm respectively. Deformation of nanotubes as well as nanotubes assembling and the formation of nanoribbons appear clearly for high reaction times (120 h).