Relationship Between K_trans and K₁ with Simultaneous Versus Separate MR/PET in Rabbits with VX2 Tumors

Lee, K. H.,Kang, S. K.,Goo, J. M.,Lee, J. S.,Cheon, G. J.,Seo, S.,Hwang, E. J. INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH 2017 Anticancer research Vol.37 No.3

<P>Background/Aim: To compare the relationship between Ktrans from DCE-MRI and K1 from dynamic (NNH3)-N-13- PET, with simultaneous and separate MR/PET in the VX-2 rabbit carcinoma model. Materials and Methods: MR/PET was performed simultaneously and separately, 14 and 15 days after VX-2 tumor implantation at the paravertebral muscle. The Ktrans and K-1 values were estimated using an in-house software program. The relationships between Ktrans and K-1 were analyzed using Pearson's correlation coefficients and linear/non-linear regression function. Results: Assuming a linear relationship, Ktrans and K-1 exhibited a moderate positive correlations with both simultaneous ( r=0.54-0.57) and separate ( r=0.53-0.69) imaging. However, while the Ktrans and K-1 from separate imaging were linearly correlated, those from simultaneous imaging exhibited a non-linear relationship. The amount of change in K-1 associated with a unit increase in Ktrans varied depending on Ktrans values. Conclusion: The relationship between K-trans and K-1 may be mis-interpreted with separate MR and PET acquisition.</P>

6'-O-Caffeoyldihydrosyringin isolated from Aster glehni suppresses lipopolysaccharide-induced iNOS, COX-2, TNF-α, IL-1β and IL-6 expression via NF-κB and AP-1 inactivation in RAW 264.7 macrophages

Seo, S.,Lee, K.G.,Shin, J.S.,Chung, E.K.,Lee, J.Y.,Kim, H.J.,Lee, K.T. Pergamon Press 2016 Bioorganic & medicinal chemistry letters Vol.26 No.19

Previously, we found that ethyl acetate extract fraction of Aster glehni exhibited anti-hyperuricemic effects in animal models and also five new caffeoylglucoside derivatives were isolated from this fraction. In this work, we evaluated the anti-inflammatory effects of these caffeoylglucoside derivatives and found that 6'-O-caffeoyldihydrosyringin (2, CDS) most potently inhibited the LPS-induced production of nitric oxide (NO) and prostaglandin E<SUB>2</SUB> (PGE<SUB>2</SUB>) in RAW 264.7 macrophages. In addition, CDS was found to concentration-dependently reduce the production of NO, PGE<SUB>2</SUB>, and the pro-inflammatory cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β) induced by LPS in macrophages. Consistent with these observations, CDS concentration-dependently inhibited LPS-induced inducible nitric oxide synthase (iNOS) and cyclooxidase-2 (COX-2) expression at the protein level and also iNOS, COX-2, TNF-α, and IL-6, IL-1β expression at the mRNA level. Furthermore, CDS suppressed the LPS-induced transcriptional activities of nuclear factor-κB (NF-κB) and activator protein-1 (AP-1) as well as the phosphorylation of p65 and c-Fos. Taken together, these results suggest that the anti-inflammatory effect of CDS is associated with the downregulation of iNOS, COX-2, TNF-α, IL-1β, and IL-6 expression via the negative regulation of NF-κB and AP-1 activation in LPS-induced RAW 264.7 macrophages.

거대아에 대한 임상적 고찰

이언기,김기영,서정식,조용균,전명권,최훈,김복린,미홍균 인제대학교 1993 仁濟醫學 Vol.14 No.3

거대아에서 임신, 분만, 또는 산후에 산모나 태아에게 발생할 수 있는 여러가지 합병증을 감소시키고 차후의 거대아 관리에 도움이 되기 위하여 거대아에 관한 임상적 고찰을 실시하게 되었다. 본 논문에서 나타난 거대아의 빈도는 6.46%로 다른 저자들의 보고와 상당한 차이가 있었으며 산모의 주된 합병증은 산도의 열상이었고 신생아의 주된 합병증은 견갑난산으로 나타났으며 2례의 태아사망이 있었다. 거대아에 대한 정확한 진단과 적절한 분만방법의 선택, 주의 깊은 산모관찰을 실시하여 거대아 분만에 따른 합병증 및 태아사망을 감소시킬 수 있을 것으로 사료된다. Ⅰ.서론 Ⅱ.연구재료 및 방법 Ⅲ.연구결과 Ⅳ.고찰 Ⅴ.결론

Improvement of the phosphoenolpyruvate carboxylase activity of Phaeodactylum tricornutum PEPCase 1 through protein engineering

Chang, K.S.,Jeon, H.,Seo, S.,Lee, Y.,Jin, E. IPC Science and Technology Press ; Elsevier Scienc 2014 Enzyme and microbial technology Vol.60 No.-

In order to mitigate CO<SUB>2</SUB> accumulation and decrease the rate of global warming and climate change, we previously presented a strategy for the development of an efficient CO<SUB>2</SUB> capture and utilization system. The system employs two recombinant enzymes, carbonic anhydrase and phosphoenolpyruvate carboxylase, which were originated from microalgae. Although utilization of this integrated system would require a large quantity of high quality PEPCase protein, such quantities could be produced by increasing the solubility of the Phaeodactylum tricornutum PEPCase 1 (PtPEPCase 1) protein in the Escherichia coli heterologous expression system. We first expressed the putative mitochondria targeting peptide- and chloroplast transit peptide-truncated proteins of PtPEPCase 1, mPtPEPCase 1 and cPtPEPCase 1, respectively, in E. coli. After affinity chromatography, the amount of purified PEPCase protein from 500mL of E. coli culture was greatest for cPtPEPCase 1 (1.99mg), followed by mPtPEPCase 1 (0.82mg) and PtPEPCase 1 (0.61mg). Furthermore, the enzymatic activity of mPtPEPCase 1 and cPtPEPCase 1 showed approximately 1.6-fold (32.19 units/mg) and 3-fold (59.48 units/mg) increases, respectively. Therefore, cPtPEPCase 1 purified using the E. coli heterogeneous expression system could be a strong candidate for a platform technology to capture CO<SUB>2</SUB> and produce value-added four-carbon platform chemicals.

The PPARα activator fenofibrate inhibits voltage-dependent K⁺ channels in rabbit coronary arterial smooth muscle cells

Li, H.,Shin, S.E.,Seo, M.S.,An, J.R.,Jung, W.K.,Ha, K.S.,Han, E.T.,Hong, S.H.,Bang, H.,Bae, Y.M.,Firth, A.L.,Choi, I.W.,Park, W.S. North-Holland 2017 European journal of pharmacology Vol.812 No.-

<P>We examined the effects of the PPAR alpha activator fenofibrate on voltage-dependent K+ (Kv) channels using a patch clamp technique in native rabbit coronary arterial smooth muscle cells. Kv current was inhibited by application of fenofibrate in a concentration-dependent manner, with an apparent IC50 value of 6.39 +/- 0.53 mu M and a slope value (Hill coefficient) of 1.63 +/- 0.10. Fenofibrate accelerated the decay rate of Kv channel inactivation. The rate constants of association and dissociation for fenofibrate were 0.81 +/- 0.05 mu M-1 s(-1) and 4.70 +/- 0.47 s(-1), respectively. Although fenofibrate did not affect the steady-state activation curves, fenofibrate shifted the inactivation curves toward a more negative potential. Application of train pulses (1 or 2 Hz) progressively increased the fenofibrate-induced inhibition of the Kv channel, and the recovery time constant from inactivation was increased in the presence of fenofibrate, which suggested that the inhibitory effect of fenofibrate is use-dependent. Another PPAR alpha activator, bezafibrate and PPARa inhibitor, GW 6471, did not affect the Kv current and also did not change the inhibitory effect of fenofibrate on the Kv current. From these results, we suggest that fenofibrate inhibited Kv current in a state-, time-, and use-dependent manner, completely independent of PPAR alpha activation.</P>

다공성 Si의 발광 특성

서영훈 ( Y. H. Seo ),남기석 ( K. S. Nahm ),서은경 ( E. K. Suh ),이영희 ( Y. H. Lee ) 한국공업화학회 1992 한국공업화학회 연구논문 초록집 Vol.1992 No.0

Si은 지금까지는 그것의 간접천이형 특성 때문에 빛을 효율성있게 내는 것이 어려워 발광소자로 이용되기가 불가능한 재료로 알려져 왔다. 그러나 영국의 Canham 그룹이 미국 응용물리학회지(Applied Physics Letters)에 다공질 Si으로 부터 고효율 적생광 발광을 처음으로 보고한 이래 1991년 프랑스의 Grenoble대학, 미국의 AT&T 및 IBM 연구그룹들이 각기 이 현상의 재현성을 실험적으로 확인함으로서 광소자 재료로서 Si 반도체의 이용 가능성을 시사하였다. 그 이후 1991년 12월에 열린 미국 재료학회(Materials Research Society)와 1992년 3월에 개최된 미-일 물리학회에서 발광 Si에 관한 수십편의 논문이 발표되고 있어, 초기단계 이기는 하지만 발광 Si의 연구가 가속화되고 있는 실정이다. 이러한 이유로 발광 다공성 Si의 생성기구, 발광기구 및 구조 등에 관해서는 분명한 결론이 내려지지 못한 실정이다. 본 연구에서는 HF수용액 속에서 p형 Si기판을 양극반응(anodization)시켜 다공성 Si(PSL: porous silicon layer)을 제조하여 PL(photoluminescence)와 Raman측정을 하여 PSL의 발광특성과 구조를 조사하였다. 또한 IR을 이용하여 PSL표면에 결합된 수소의 결합상태와 porosity를 측정하여 PSL의 발광기구를 규명하고자 노력하였다. 그 결과 PL peak는 1.8 eV 근처에서 보였으며, 그 강도는 5 K에서 얻은 GaAs의 PL 강도와 거의 같았다.

Suppression of PPARγ through MKRN1-mediated ubiquitination and degradation prevents adipocyte differentiation

Kim, J-H,Park, K W,Lee, E-W,Jang, W-S,Seo, J,Shin, S,Hwang, K-A,Song, J Macmillan Publishers Limited 2014 Cell death and differentiation Vol.21 No.4

The central regulator of adipogenesis, PPARγ, is a nuclear receptor that is linked to obesity and metabolic diseases. Here we report that MKRN1 is an E3 ligase of PPARγ that induces its ubiquitination, followed by proteasome-dependent degradation. Furthermore, we identified two lysine sites at 184 and 185 that appear to be targeted for ubiquitination by MKRN1. Stable overexpression of MKRN1 reduced PPARγ protein levels and suppressed adipocyte differentiation in 3T3-L1 and C3H10T1/2 cells. In contrast, MKRN1 depletion stimulated adipocyte differentiation in these cells. Finally, MKRN1 knockout MEFs showed an increased capacity for adipocyte differentiation compared with wild-type MEFs, with a concomitant increase of PPARγ and adipogenic markers. Together, these data indicate that MKRN1 is an elusive PPARγ E3 ligase that targets PPARγ for proteasomal degradation by ubiquitin-dependent pathways, and further depict MKRN1 as a novel target for diseases involving PPARγ.

특별강연(特別講演) 및 학술연구(學術硏究) 발표요지(發表要旨) : 제2분야: 잡초 생리생태 ; protox저해형 신규화합물 K-15557의 제초활성과 체내이행성

서지예 ( J. Y. Seo ),고영관 ( Y. K. Ko ),류재욱 ( J. W. Ryu ),김대황 ( D. H. Kim ),송재은 ( J. E. Song ),정봉진 ( B. J. Jung ),김태준 ( T. J. Kim ) 한국잡초학회 2005 한국잡초학회 별책(학술대회 초록집) Vol.25 No.2

EZH2 Generates a Methyl Degron that Is Recognized by the DCAF1/DDB1/CUL4 E3 Ubiquitin Ligase Complex

Lee, J.,Lee, Jason S.,Kim, H.,Kim, K.,Park, H.,Kim, J.Y.,Lee, S.,Kim, I.,Kim, J.,Lee, M.,Chung, C.,Seo, S.B.,Yoon, J.B.,Ko, E.,Noh, D.Y.,Kim, K.,Kim, K.,Baek, S. Cell Press 2012 Molecular cell Vol.48 No.4

Ubiquitination plays a major role in protein degradation. Although phosphorylation-dependent ubiquitination is well known for the regulation of protein stability, methylation-dependent ubiquitination machinery has not been characterized. Here, we provide evidence that methylation-dependent ubiquitination is carried out by damage-specific DNA binding protein 1 (DDB1)/cullin4 (CUL4) E3 ubiquitin ligase complex and a DDB1-CUL4-associated factor 1 (DCAF1) adaptor, which recognizes monomethylated substrates. Molecular modeling and binding affinity studies reveal that the putative chromo domain of DCAF1 directly recognizes monomethylated substrates, whereas critical binding pocket mutations of the DCAF1 chromo domain ablated the binding from the monomethylated substrates. Further, we discovered that enhancer of zeste homolog 2 (EZH2) methyltransferase has distinct substrate specificities for histone H3K27 and nonhistones exemplified by an orphan nuclear receptor, RORα. We propose that EZH2-DCAF1/DDB1/CUL4 represents a previously unrecognized methylation-dependent ubiquitination machinery specifically recognizing ''methyl degron''; through this, nonhistone protein stability can be dynamically regulated in a methylation-dependent manner.

대학생의 문제음주, 사회적 지지, 우울에 관한 연구

권민정,김리예,김세영,김은진,서혜인,심누리,이혜진,천경민,최하은,신주현,이민경 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: The purpose of this study was to examine the correlation of alcohol use disorder, social support and depression of the college students. Method: In this descriptive design study, 334 university students were recruited through convenient sampling from October, 2015 to January, 2016. Questionnaires used for online Google docs were AUDIT-K(Alcohol Use Disorders Identification Test-Korea), MSPSS(Multidimensional Scale of Perceived Social Support) and SDS(Self - Rating Depression Scale). Data were analyzed with descriptive statistics, independent t-test, one way ANOVA with Scheffe's test, and Pearson’s coefficient using SPSS 22.0 program. Result: 230 students of the 334 college students(68.8%) were classified into alcohol use disorder group. There was a significant correlation between the degree of social support and depression among all participants(r=-.45, p=.000). However, there was no correlation between alcohol use disorder and depression(r=.23, p=.006) and between alcohol use disorder and social support(r=.02, p=.758). Conclusion: This study showed a relationship between social support and depression, not the alcohol use disorder. This result can be used as the basic data in nursing research about alcohol use disorder, social support and depression of college students. Also, Further researches are needed to examine the factors that affect alcohol use disorder of college students, as well as depression and social support.