Cloning and Expression of 34-kiIodalton protein Gene of Mycobacterium pamtuberculosis

Doo Kim,Yung-Fu Chang,Sang-Jae Shin 대한수의학회 1994 대한수의학회 학술대회발표집 Vol.1994 No.-

Molecular Phylogenetic Classification of Dermatophytes Isolated from Dogs and Cats

Doo Kim,Seok-Young Jeoung,So-Jeo Ahn 한국임상수의학회 2006 한국임상수의학회지 Vol.23 No.4

Using internal transcribed spacer 1 (ITS1) region ribosomal DNA sequences from 9 strains of Microsporumcanis and 5 strains of Microsporum gypseum isolated from dogs and a cat with dermatophytosis, we demonstratedthe mutual phylogenetic relationship of these strains. Nucleotide sequence analysis of the ITS1 gene fragments fromthe 9 strains of M canis had the 100% nucleotide sequence similarities and the 5 strains of M gypseum also hadthe 100% nucleotide sequence similarities. The phylogenetic analysis of the nucleotide sequences of the 9 strains ofM canis formed a nested cluster with the reference strains of M canisEurope. M canis were genetically distinct from the other reference strains of Microsporum spp, but M distortum, Mequinum, and M ferrugineum were genetically very close to M canis. M gypseum form a cluster in the phylogenetictree with M canis as an outgroup. The molecular analysis of ITS1 genes provided the useful information for theidentification of these Microsporum species and the understanding of their relationship.

Epidemiological Observation on Recent Outbreaks of Canine Distemper in Korea

Doo Kim,Son-il Pak,Ji-young Park,So-jeo Ahn,Seok-young Jeoung 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.3

To characterize the recent outbreaks of canine distemper (CD) in Korea, we carried out epidemiologicalinvestigations by clinical observations, serum neutralizing (SN) antibody titer determination and RT-PCR on the 315 dogswere infected with CDV. Breed or gender did not sem to have effects on the prevalence of CD. The major part ofdogs were in young age from 6 weeks to 18 weeks of age, and were not vaccinated or incompletely vaccinated. Clinicalsigns of dogs with CD were multisystemic and extremely variable. Dogs died from CD had significantly more ocularsigns and neurologic signs than those of dogs survived (p<0.05). The SN titers against CDV of 157 (96.9%) dogs wereunder 1:16, which is less than protective level. One possible explanation for recent outbreaks of CD in Korea mightbe low antibody titers against CDV because of vacination failure. Therefore, to reduce the impact of virulent infectionin the dog population, dogs should be vacinated adequately and prophylactic measures should include isolation of youngdogs from the dog population until vaccination can be expected to provide protection. 본 연구에서는 최근 국내에서 발병하는 개 디스템퍼(CD)의 역학적 조사를 위하여 2002년 2월부터 2002년 10월까지 강원도, 경기도와 서울특별시의 10개 동물병원 수의사에 의하여 CD에 감염된 것으로 잠정진단된 315마리의 개를 대상으로 하였다. 개체의 신상자료와 임상증상을 조사하였으며 CD를 확진하기 위하여 혈액의 단핵구나 결막 상피세포에서 CDV의 NP gene을 RT-PCR로 증폭하였다. CD로 의심되는 총 315마리 중 162마리가 CD 양성으로 진단 되었으며 성별과 종에 따른 발병율에는 차이가 없었다. 나이별 분포는 백신을 접종해야 하는 기간인 6-18주에 가장 높은 분포를 보였으며 1세 이상에서도 소수 발병하였다. CD 환축은 호흡기 증상, 위장관 증상, 눈의 증상 등 다양한 증상이 나타나 임상증상에 근거하여 CD를 진단하기에 어려움이 있었다. CD 양성의 개 중 84.6%가 백신을 한번도 맞지 않았거나 예방접종을 제대로 실시되지 않은 상태였으며 CDV에 대한 혈청 중화 항체가는 96.9%의 개가 방어수준 이하인 1:16 이하를 나타내었다. CD 양성 162마리와 CD음성으로 진단 된 153마리의 혈청 중화 항체가 사이에는 통계적인 유의차가 인정되었으며 (P< 0.05), CD 양성 개 중에 생존 한 개들은 폐사한 개들보다 중화 항체가 높은 경향을 보였다. 눈의 증상과 신경증상을 보인 개체들은 다른 개체들에 비해 생존율이 유의하게 낮은 것으로 나타났다 (P< 0.05). 본 연구의 결과 현재 국내에서 발병하고 있는 개 디스템퍼는 예방접종의 실패에 따라 개들의 항체 수준이 전반적으로 낮은 것에 기인하는 것으로 판단되므로 예방접종에 대한 전반적인 검토가 필요하며 예방을 위하여 감염에 취약한 시기에는 감염원에 접촉을 최소화하는 것이 중요할 것으로 판단되었다.

전분 제조방법에 따른 밤전분의 이화학적 특성

김용두(Yong?Doo Kim),최옥자(Ok?Ja Choi),심기훈(Ki?Hoon Shim),조인경(In?Kyung Cho) 한국식품영양과학회 2006 한국식품영양과학회지 Vol.35 No.3

알칼리 침지법에 의하여 박피한 밤 전분과 박피하지 않은 밤 전분의 이화학적 특성, 가열 및 알칼리에 의한 호화특성을 각각 분석한 결과는 다음과 같다. 물결합력은 박피한 A전분의 경우 86.9%, 박피하지 않은 B전분은 80.66%로 박피한 A전분이 더 높은 물결합력을 나타냈다. A전분과 B전분의 팽윤력은 60℃에서 급격히 증가하기 시작하여 80℃까지 증가하다가 그 이후는 완만하게 변하였고, 용해도는 60℃에서 부터 증가하기 시작하여 온도가 상승함에 따라 점점 증가하는 특성을 보였으며, A전분이 B전분보다 팽윤력과 용해도가 더 높게 나타났다. 요오드 반응은 A전분이 B전분보다 더 높게 났다. X-선 회절도는 A전분은 Cb도형, B전분은 B도형으로 나타났고, 상대적 결정화도는 A전분이 36.2%, B전분은 37.0%로 B전분이 더 높게 나타났다. DSC에 의하여 측정한 호화온도 및 호화엔탈피는 A전분의 경우 66.95℃~77.5℃, 2.04 ㎈/g, B전분의 경우 67.09℃~77.5℃, 2.29 ㎈/g으로 나타났다. 6.5% 밤 전분의 아밀로그램 특성은 A전분보다 B전분이 호화개시온도가 더 높았고, peak viscosity, breakdown, setback은 A전분이 B전분보다 더 높았다. 알칼리에 의한 호화특성에서 A전분이 B전분보다 점도, gel 부피 및 광투과도가 더 높게 나타났다. This study is to investigate the physicochemical properties of differently pretreated chestnut starches during starch isolation, and to examine their gelatinization properties by both heat and alkali treatments. One kind is starch A made by alkali method from peeled chestnut. The other is starch B made from chestnut with the outer layer. The results are as follows. Starch A has higher water binding capacity of 86.9% than starch B with 80.66%. Swelling powers of both starch A and B increased rapidly from 60℃ to 80℃ in both, and since then it has changed a bit. Both began to show their solubility at 60℃ and increased continuously as the temperature went up. Starch A has higher swelling power and solubility than starch B. In iodine reaction, starch A has higher λmax and absorbance at λmax than starch B. X?ray diffraction patterns showed that starch A is type Cb and that starch B is type B. Starch B has higher relative crystallinity of 37.0% than starch A with 36.2%. The results by differential scanning calorimetry revealed that starch A gelatinized from 66.95℃ to 77.5℃, and its enthalpy is 2.04 ㎈/g. And starch B gelatinized from 67.09℃ to 77.5℃, and its enthalpy is 2.29 ㎈/g. Amylograms of chestnut starch at 6.5% concentration indicated that starch B needs higher onset temperature when beginning to gelatinize than starch A does. But starch A shows much higher peak viscosity, breakdown, and setback than starch B does. Starch A shows higher viscosity, gel volume, and optical transmittance in gelatinization properties by alkali than starch B does.

Estimation of Paratuberculosis Prevalence in Dairy Cattle in Province of Korea using an Enzyme-linked Immunosorbent Assay:Application of Bayesian Approach

Son-ilPak,DooKim,MoSalman 대한수의학회 2003 Journal of Veterinary Science Vol.4 No.1

To draw inferences about the sensitivity andspecificity of the newly developed ELISA test forbovine paratuberculosis (PTB) diagnosis and posteriordistribution on the prevalence of PTB in a provinceof Korea, we applied Bayesian approach with Gibbssampler to the data extracted from the prevalencestudy in 1999.The data were from a single test resultswithout a designated gold test.The prevalence estimates for PTB in study po-pulation ranged 3.2∼5.3% for conservative and 6.7∼7.1% for liberal, depending on the priors used. Thesimulated specificities of the ELISA close to oneanother, ranging 84.7∼90.6% , whereas the sensitivitywas somewhat spread out depending largely on thepriors with a range of46.4∼88.2% .Our findings indicatethat the ELISA method appeared useful as a screeningtool at a minimum level in comparison to otherdiagnostic tests available for this disease in terms ofsensitivity. However, this advantage comes at a costof having low specificity of the test.

Diagnostic Performance for Detection of Helicobacter Pylori Infection in Gastric Biopsy Specimens with No Gold Test: Non-linear Regression Approach

Son-IlPak,DooKim 한국임상수의학회 2003 한국임상수의학회지 Vol.20 No.1

The selection of a test as a reference with no perfect sensitivity and specificity may lead to bias, yielding distortion of the diagnostic performance. This means it is inappropriate to use imperfect diagnostic tests as a reference method to identify infected patients in clinical environments. In this study, diagnostic performance of rapid urease test, polymerase chain reaction (PCR), and histology of gastric biopsy specimens for diagnosing Helicobacter pylori infection separately and in combination was estimated by using non-linear regression. Based on this approach, the sensitivity, specificity and likelihood ratio positive and negative values for each test were as follows: urease test 99.9%, 99.9%, 99.9%, 99.6%, respectively; PCR 88.6%, 99.9%, 99.9%, 70.5%, respectively; histology 78.3%, 97%, 78.3%, 97%, respectively. Predictive values for positive and negative changes with varying prevalences. A positive histology test will be correct at least 90% of the time provided prevalence of H. pylori is greater than 35%, whereas a negative test will be correct in more than 90% if the prevalence is less than 25%. Combination of three diagnostic tests employed in the study gives no substantial benefit for practitioners to screen infected patients, and urease test or PCR represents an appropriate single test in clinical environments.

Effect of Si/α-Si3N4 Ratio on the Shape of Silicon Nitride Particles Produced by SHS Method

Min-SungKim,Dong-SooPark,Byung-DongHan,Hai-DooKim,ChanPark 한국세라믹학회 2002 한국세라믹학회지 Vol.39 No.3

Effect of the Amount of the Sintering Additives on the Microstructural Development and the Mechanical Properties of Silicon Nitride with Aligned Whisker Seeds

Byoung-ChanBae,Dong-SooPark,Hai-DooKim,Byung-DongHan,ChanPark,LinhuaZou 한국세라믹학회 2002 한국세라믹학회지 Vol.39 No.8

Induction of hepatic inducible nitric oxide synthase by cholesterol in vivo and in vitro

Jun-WooKim,Keon-WookKang,GooTaegOh,JihyunSong,Nak-DooKim,김영미 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.2

Cholesterol-rich diet impairs endothelial NO syn-thase (eNOS) and enhances inducible NOS (iNOS)expresion. In this study, we investigated efects ofcholesterol on iNOS expression in high-fat-fed ratmodels, HepG2 and RAW264.7 cels. The high-fatdiet increased the plasma total cholesterol level 6-7fold and low-density lipoprotein cholesterol level(LDL-C) approximately 70 fold and slightly increasedthe level of lipid peroxidation as determined bythiobarbituric acid-reactive substance assay. Thehigh-fat diet also increased plasma nitric oxide (NO)concentrations up to 5 fold, and induced iNOSmRNA expression in liver. The contractile responsesof the endothelium-denuded thoracic aortic rings tophenylephrine were significantly damaged in high-fat-fed rats when assessed by organ chamber study.Treatment with estrogen for 4 days failed to reduceiNOS expresions as well as aortic contractility,although it improved lipid profiles. In culturedHepG2 or murine macrophage RAW264.7 cells, 3days treatment with either 25-hydroxycholesterol or7-ketocholesterol induced iNOS mRNA expression,as determined by RT-PCR. Our data suggested thatthe chronic exposure of hepatocytes and macroph-age cells to high concentration of cholesterol oroxysterols may induce iNOS expression and subse-quent synthesis of NO, which may be important inthe pathogenesis of atherosclerosis.