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Youping Sun,Lois Berg Stack,Donglin Zhang,Zhanying Gu 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.4
Ethephon was sprayed on Euphorbia pulcherrima Willd. ex Klotzsch (poinsettia) ‘Sonora Jingle’ and ‘Sonora White’ to control their height and produce more compact potted plants. The results showed that Ethephon could effectively control the growth of ‘Sonora Jingle’ and ‘Sonora White’ poinsettia. Height was reduced by was 33.4 ± 0.8% for ‘Sonora Jingle’ and 30.8 ± 1.3% for ‘Sonora White’ poinsettia, when 700 mg・L^(-1) Ethephon was sprayed three times on 29 August, 20September, and 13 October 2005, respectively. Similar to other plant growth retardants, side effects including phytotoxicity and delays to first bract color were also observed on Ethephon treated poinsettia. However, all plants produced were still of a marketable quality.
Genome-wide association study for loin muscle area of commercial crossbred pigs
Luan Menghao,Ruan Donglin,Qiu Yibin,Ye Yong,Zhou Shenping,Yang Jifei,Sun Ying,Ma Fucai,Wu Zhenfang,Yang Jie,Yang Ming,Zheng Enqin,Cai Gengyuan,Huang Sixiu 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6
Objective: Loin muscle area (LMA) is an important target trait of pig breeding. This study aimed to identify single nucleotide polymorphisms (SNPs) and genes associated with LMA in the Duroc×(Landrace×Yorkshire) crossbred pigs (DLY). Methods: A genome-wide association study was performed using the Illumina 50K chip to map the genetic marker and genes associated with LMA in 511 DLY pigs (255 boars and 256 sows). Results: After quality control, we detected 35,426 SNPs, including six SNPs significantly associated with LMA in pigs, with MARC0094338 and ASGA0072817 being the two key SNPs responsible for 1.77% and 2.48% of the phenotypic variance of LMA, respectively. Based on previous research, we determined two candidate genes (growth hormone receptor [GHR] and 3-oxoacid Co A-transferase 1 [OXCT1]) that are associated with fat deposition and muscle growth and found further additional genes (MYOCD, ARHGAP44, ELAC2, MAP2K4, FBXO4, FBLL1, RARS1, SLIT3, and RANK3) that are presumed to have an effect on LMA. Conclusion: This study contributes to the identification of the mutation that underlies quantitative trait loci associated with LMA and to future pig breeding programs based on marker-assisted selection. Further studies are needed to elucidate the role of the identified candidate genes in the physiological processes involved in LMA regulation.
Sihang Bao,Junzhen Di,Jianguo Yang,Donglin Wang,Juan Sun,Yanrong Dong 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.3
In view of the high content of Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> in acid mine drainage (AMD), the adsorption properties of lignite for Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> were studied. The adsorption performance of lignite for Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> was revealed by combining with FT-IR, XPS and EDS. The results showed that the adsorption kinetic model of lignite for Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> conformed to the quasi-first-order kinetic model. The isothermal adsorption line fitting models of lignite for Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> were in accordance with the Langmuir model and the Freundlich model, respectively. The maximum equilibrium adsorption capacities of lignite for Cu and Zn were 67.84 mg/g and 55.5 mg/g. The adsorption process of Cu<SUP>2+</SUP> and Zn<SUP>2+</SUP> by lignite involved electrostatic, coordination and ion exchange. Under the condition of coexistence of two kinds of ion, the adsorption site of lignite had stronger binding ability to copper ions.
Jingcui Yu,Songbin Fu,Peng Liu,Xiaobo Cui,Yu Sui,Guohua Ji,Rongwei Guan,Donglin Sun,Wei Ji,Fangli Liu,An Liu,Yuzhen Zhao,Yang Yu,Yan Jin,Jing Bai,Jingshu Geng,Yingwei Xue,Jiping Qi,Ki-Young Lee 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.1
Previously, we identified 3 overlapping regions showing loss of heterozygosity (LOH, R_1-R_3 from 11 to 30 cM) on chromosome 17 in 45 primary gastric cancers (GCs). The data indicated the presence of tumor suppressor genes (TSGs) on chromosome 17 involved in GC. Among the putative TSGs in these regions, HIC1 (in SR_1) and TOB1 (in SR_3) remain to be examined in GC. By immunohistochemistry (IHC), methylation-specific PCR (MSP) and western blot, we evaluated the expression and regulation status for HIC1 and TOB1 protein in GC. We narrowed down the deletion intervals on chromosome 17 and defined five smaller LOH subregions, SR_1-SR_5 (0.54 to 3.42 cM), in GC. We found that HIC1 had downregulated expression in 86% (91/106) and was methylated in 87% (26/30) of primary GCs. Of the primary GCs showing downregulation of HIC1 protein, 75% (18/24) had methylated HIC1 gene. TOB1 was either absent or expressed at reduced levels in 75% (73/97) of the GC samples. In addition, a general reduction was found in total and the ratio of unphosphorylated to phosphorylated TOB1 protein levels in the differentiated GC cell lines. Further analysis revealed significant simultaneous downregulation of both HIC1 and TOB1 protein in GC tissue microarray samples (67%, 52/78) and in primary GCs (65%, 11/17). These results indicate that silencing of HIC1 and TOB1 expression is a common occurrence in GC and may contribute to the development and progression of the disease.