Evidence of the genetic diversity and clonal population structure of Oenococcus oeni strains isolated from different wine-making regions of China

Dongliang Yu,Kan Shi,Xiangyuan Wen,Fangshu Xie,Tao Wang,Shuwen Liu,Ling He 한국미생물학회 2018 The journal of microbiology Vol.56 No.8

Studies of the genetic diversity and population structure of Oenococcus oeni (O. oeni) strains from China are lacking compared to other countries and regions. In this study, amplified fragment length polymorphism (AFLP) and multilocus sequence typing (MLST) methods were used to investigate the genetic diversity and regional evolutionary patterns of 38 O. oeni strains isolated from different wine-making regions in China. The results indicated that AFLP was markedly more efficient than MLST for typing O. oeni strains. AFLP distinguished 37 DNA patterns compared to 7 sequence types identified using MLST, corresponding to discriminatory indices of 0.999 and 0.602, respectively. The AFLP results revealed a high level of genetic diversity among the O. oeni strains from different regions of China, since two subpopulations and an intraspecific homology higher than 60% were observed. Phylogenetic analysis of the O. oeni strains using the MLST method also identified two major phylogroups, which were differentiated into two distinct clonal complexes by minimum spanning tree analysis. Neither intragenic nor intergenic recombination verified the existence of the clonal population structure of the O. oeni strains.

Enhanced Strength-Ductility Synergy in Mg-0.5 wt%Ce Alloy by hot Extrusion

Hui Yu,Yu Liu,Ya Liu,Dongliang Wang,Yuling Xu,Binan Jiang,Weili Cheng,Lixin Huang,Weineng Tang,Wei Yu 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.7

The microstructure and mechanical properties of micro-alloyed Mg-0.5 wt%Ce alloys are investigated systemically in this study. It shows that Mg-Ce binary system is very sensitive to extrusion temperature. On the one hand, the high tensile yield strength (TYS) of 343 MPa was obtained by extrusion at 250 °C mainly due to the combined effects of profuse nano-precipitation phase, grain refinement, texture strengthening and high-density dislocation; On the other hand, the excellent elongation of 30% was acquired by extrusion at 300 °C resulting from the elimination of dislocations as well as the modification of texture.

Molecular dynamics simulation of nanoindentation influence of indenter velocity on 3C-SiC ceramics

Yu Dongling,Liu Dongliang,Yi Jiaqi,Zhou Jianzhen,Wu Nanxing 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.2

To investigate the effect of nanoindentation velocity on dislocation nucleation, development and elastic deformation of 3C-SiCceramic specimens, the nanoindentation loading process of 3C-SiC ceramic specimens under a diamond indenter is simulated. The molecular dynamics models of diamond indenter and 3C-SiC amorphous layer are constructed, and the nanoindentationconditions such as potential function, relaxation synthesis and time step are optimized. The dislocation analysis method andthe identify diamond structure method are used to analyze the internal structure change of the specimen and the dislocationgeneration, change and transfer of different positions after nanoindentation simulation. It is found that with the increase ofindenter velocity, the elastic threshold decreases and the plasticity increases, and tends to be stable at a certain stage. The loadfluctuation tends to be flat, the total amount of dislocation generated continues to decrease, and the diffusion rate of dislocationslows down. The length of 1/2<110> and 1/6<112> type dislocation becomes shorter, indentation formation of the 'lotus' shapemore regularly. The elastic-plastic threshold of 3C-SiC ceramic material also changes with the change of indenter velocity, andthe dislocation formation rate and total amount also affect while show a certain regularity.

Facial Action Unit Detection with Multilayer Fused Multi-Task and Multi-Label Deep Learning Network

( Jun He ),( Dongliang Li ),( Sun Bo ),( Lejun Yu ) 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.11

Facial action units (AUs) have recently drawn increased attention because they can be used to recognize facial expressions. A variety of methods have been designed for frontal-view AU detection, but few have been able to handle multi-view face images. In this paper we propose a method for multi-view facial AU detection using a fused multilayer, multi-task, and multi-label deep learning network. The network can complete two tasks: AU detection and facial view detection. AU detection is a multi-label problem and facial view detection is a single-label problem. A residual network and multilayer fusion are applied to obtain more representative features. Our method is effective and performs well. The F1 score on FERA 2017 is 13.1% higher than the baseline. The facial view recognition accuracy is 0.991. This shows that our multi-task, multi-label model could achieve good performance on the two tasks.

Effects of exposure to trace pyriproxyfen on the intestinal bacterial diversity and immune signal pathways of silkworm (Bombyx mori) larvae

Lu Qingyu,Li Guoli,Lan Huangli,Yu Dongliang,Yin Xingcan,Yang Wenqi,Yang Zehao,He Chunhui,Mo Danmei,Xu Kaizun 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.2

Silkworm (Bombyx mori) is a widely used lepidopteran model insect. Pyriproxyfen is an insect growth regulator (IGR) that can be used to control pests such as lepidoptera and diptera, and there is no research about its in fluence on the intestinal bacterial diversity and immune signal pathway of non-target lepidopteran insect larvae. In this study, we treated fifth-instar silkworms with trace pyriproxyfen (10 − 4 mg/L). The results showed that the abundance and diversity of silkworm larvae intestinal flora at the level of phylum, class, order, family and genus were all changed. The function prediction result showed that the functional changes mainly focused on tran scription, amino acid transport and metabolism, carbohydrate transport and metabolism, signal transduction mechanisms, energy production and conversion et al. after exposure to trace pyriproxyfen. Furthermore, qRTPCR results showed that trace pyriproxyfen exposure activated the transcriptions of antimicrobial peptides (AMPs) genes regulated by Toll and IMD pathways. This research can lay the foundation for the study on the resistance of lepidopteran insects to pyriproxyfen and other juvenile hormone pesticides.

Effect of Phosphodiesterase-5 Inhibitors on the Treatment of Male Infertility: A Systematic Review and Meta-Analysis

Dong Liang,Zhang Xiaojin,Yan Xuhong,Shen Yifeng,Li Yulin,Yu Xujun 대한남성과학회 2021 The World Journal of Men's Health Vol.39 No.4

Purpose: Male infertility is a worldwide problem with limitations in the treatment. Phosphodiesterase-5 inhibitors (PDE5is) is the first choice for the treatment of erectile dysfunction, more and more studies show that it has a certain effect on male infertility in recent years. But there was currently no high quality of systematic review to evaluate the effects of PDE5is on semen quality. Materials and Methods: We retrieved the electronic databases of MEDLINE, PubMed, Web of Science, EMBASE, etc. Related randomized controlled trials (RCTs) were collected and selected up to May 20, 2020. We have searched literature with terms “male infertility”, “phosphodiesterase-5 inhibitors”, “PDE5i”, “Tadalafil”, “Sildenafil”, “Vardenafil”, “Udenafil”, “Avanafil”, “semen”, and “sperm”. Mean value and its standard deviation were used to perform quantitative analysis. All statistical analyses were conducted by RevMan 5.3 and Stata software. Results: There were a total of 1,121 participants in the nine included studies. There was a statistically significant improvement treated with PDE5is compared with sham therapy, which including sperm concentration (mean difference [MD]=1.96, 95% confidence interval [CI]=1.70–2.21, p<0.001; MD=3.22, 95% CI=1.96–4.48, p<0.001), straight progressive motility (%) Grade A (MD=3.71, 95% CI=2.21–5.20, p<0.001), sperm motility (MD=8.09, 95% CI=7.83–8.36, p<0.001), morphologically normal spermatozoa (%) (MD=0.67, 95% CI=0.20–1.15, p=0.005; MD=1.27, 95% CI=0.02–2.52, p=0.05), sperm abnormalities (%) (MD=-0.64, 95% CI=-0.81–-0.47, p<0.001), and progressive motile sperm (MD=5.34, 95% CI=3.87–6.81, p<0.001). Conclusions: In this meta-analysis of nine RCTs, treatment with PDE5is could improve some indicators of male sperm.