http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Implantation Rate increased by Uterus Endometrial Injury in Spontaneous Diabetes Mellitus Rat
Hoseung Kwak,Donghun Kang,Eunjoo Lee,Daeyoung Kim 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10
Embryo transfer is one of the important process of assisted reproductive technology (ART) and that is associated with uterus endometrial receptivity. Recently, mouse endometrial stimulation by artificial injury had shown the favorable effect on conception. In this experiment, we used uterus stimulation method that injury the endometrium to increase implantation rate for spontaneous Diabetes Mellitus (sDM) rat. Rats are divided into several groups involved a control group. We performed the surgical method to experimental group bilaterally or unilaterally. After that, we investigated morphological change using immunohistochemical staining method and calculated implanted embryos respective sides of the uterus. The number of implanted embryo in the experimental group was significantly higher and there were lots of morphological changes including glands and endometrial cells that support implantation. Our results showed that sDM rat uterus endometrial injury in ART help enhancing implantation rate.
이용자 특성과 재난 미디어 기능에 따른 재난 미디어 이용과 만족도
Chunsub Kwak,Donghun Chung 위기관리 이론과 실천 2014 Crisisonomy Vol.10 No.11
ype of disasters has recently become various and thus the public alert and warning system has also become diverse with the media technology development. According to the various changes of situations around disasters, this study was to investigate the status of use and the fitness of disaster information delivery function based on audience survey. The role of media in disaster was divided informative function and alerting function. As a result, Television, computer, and mobile device were the major media in the disaster situation. Television is used the most, but computer gets satisfaction the most. People who have experienced natural disaster ever showd higher disaster media usage and satisfaction, and the generation gap is identified. Younger generation use computer the most, and is satisfied with computer and mobile device as well while older generation use radio and siren the most and is satisfied with television as well. In addition, mobile device was evaluated to be the most suitable for informational function and alert function as roles of media in disaster. 최근 우리 사회의 재난 유형이 다양해지고 있으며, 재난 정보 전달체계도 미디어 기술의 발전으로 더욱 다양 해지고 있다. 따라서 본 연구에서는 이용자를 대상으로 여러 재난정보 전달 미디어의 이용 실태와 기능 적합도를 조사하고자 하였다. 재난 미디어의 기능은 정보적 기능과 경보적 기능으로 구분하였는데, 연구결과 텔레비전, 컴 퓨터, 휴대기기가 재난 상황에서의 주요 미디어로 조사되었다. 텔레비전의 이용도가 높았지만, 만족도에서는 컴퓨 터가 가장 높았으며. 자연재해 경험자들은 재난 정보의 이용과 만족도가 상대적으로 높게 나타났다. 한편 세대 차이가 확인되었는데, 젊은 세대는 컴퓨터 이용도와 컴퓨터와 휴대기기의 만족도가 상대적으로 높은 반면, 나이 든 세대에서는 라디오와 경보시설 이용도와 텔레비전 만족도가 상대적으로 높았다. 더불어 휴대기기가 재난 미디 어의 정보적 기능과 경보적 기능에서 가장 적합한 미디어로 평가되었다.
Implantation Rate Increased by Uterus Endometrial Injury in sDM Rat
Hoseung Kwak,Donghun Kang,Jihye Jang,Eunjoo Lee,Daeyoung Kim 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05
Embryo transfer is one of the important process of assisted reproductive technology (ART) and that is associated with uterus endometrial receptivity. Recently, mouse endometrial stimulation by artificial injury had shown the favorable effect on conception. In this experiment, we used uterus stimulation method that injury the endometrium to increase implantation rate for spontaneous Diabetes Mellitus (sDM) rat. Rats are divided into several groups involved a control group. We performed the surgical method to Experimental group bilaterally or unilaterally After that, we investigated morphological change and calculated implanted embryos respective sides of the uterus. The number of implanted embryo in the experimental group was significantly higher and there were lots of morphological changes including glands and endometrial cells that support implantation. Our results showed that rat uterus endometrial injury in ART help enhancing implantation rate.
Jihye Jang,Donghun Kang,Hoseung Kwak,Eunjoo Lee,Daeyoung Kim 한국수정란이식학회 2017 한국수정란이식학회 학술대회 Vol.2017 No.05
Superovulation is a technique to acquire rats to produce many eggs than normal rats. Superovulated eggs were used to make cloned animals through somatic cell nuclear transfer technology (SCNT). Healthy and valuable oocyte retrieval is essential for successful somatic cell nuclear transfer. Superovulation is also essential to maximize to the yield of IVF-derived rat eggs. Osmotic pumps (Alzet®) are miniature in order to provide research with a convenient, and reliable alternative to chronic injections. Acquiring superovulated oocytes through osmotic pump in minimizing irritation to the uterus and ovaries are competitive. We investigated the effects of pregnant mare serum gonadotropin (PMSG) and human Chorionic Gonadotropin (hCG) using osmotic pump in sDM rat. Adult female rats at 11 wks of age were used for superovulation. The response to PMSG and hCG were examined by osmotic pump of 150 IU/kg PMSG + 75 IU/kg hCG or 150 IU/kg PMSG + 150 IU/kg hCG or 300 IU/kg PMSG + 150 IU/kg hCG or 300 IU/kg PMSG + 300 IU/kg hCG. HCG was administered 48 hrs later after administration of PMSG. Oocytes were collected from the oviducts 16–18 hrs after hCG administration. Superovulation was significantly higher in rats administrated 150 IU/kg PMSG + 75 IU/kg hCG. This study demonstrated that healthy oocytes were produced in DM rat by PMSG and hCG that flowed through the osmotic pump ameliorate on uterus and ovaries.