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( Donghong Zhang ),( Dongliang Liu ),( Bing Liu ),( Xiulan Ma ) 한국미생물생명공학회(구 한국산업미생물학회) 2021 Journal of microbiology and biotechnology Vol.31 No.4
There has been increasing interest in the head and neck squamous cell carcinoma (HNSCC) that is caused by high-risk human papillomavirus (HR-HPV) and has posed a significant challenge to Otolaryngologists. A rapid, sensitive, and reliable method is required for the detection of HR-HPV in clinical specimens to prevent and treat HPV-induced diseases. In this study, a multiple cross-linking spiral amplification (MCLSA) assay was developed for the visual detection of HPV-16. In the MCLSA assay, samples were incubated under optimized conditions at 62°C for 45 min, and after mixing with the SYBR Green I (SGI) dye, the positive amplicons showed bright green fluorescence while the negative amplicons exhibited no obvious change. The specificity test revealed that the developed MCLSA technique had high specificity and could effectively distinguish all five HPV-16 strains from other pathogenic microorganisms. In terms of analytical sensitivity, the limit of detection (LoD) of MCLSA assay was approximately 5.4 × 10<sup>1</sup> copies/tube, which was 10-fold more sensitive than loopmediated isothermal amplification (LAMP) and RT-PCR. The detection results of laryngeal cancer specimens collected from 46 patients with suspected HPV infection in the Liaoning region demonstrated that the positive detection rates of MCLSA and hybridized capture 2 kit were 32.61% (15/46). The true positive rate of the MCLSA assay was higher than that of RT-PCR (100% vs. 93.33%) and LAMP (100% vs. 86.67%). Therefore, the MCLSA assay developed in the present study could be a potentially useful tool for the point-of-care (PoC) diagnosis of HR-HPV, especially in resourcelimited countries.
Efficient Order-based Scheduling Algorithms for Automated Retrieval System (ARS) in Smart Warehouses
Jialei Liu,Soung-Yue Liew,Boon Yaik Ooi,Donghong Qin 제어로봇시스템학회 2020 제어로봇시스템학회 국제학술대회 논문집 Vol.2020 No.10
A Smart Warehouse (SW) usually employs an automated warehouse management system that integrates artificial intelligence and robotics to automate the warehouse processes. For instance, an Automated Retrieval System (ARS) is used in SW to retrieve products ordered by customers from shelves in order to speed up the product movement. Such a process is important because the product retrieval speed directly affects the subsequent processes, such as packaging and delivery, thus bringing a significant impact on the entire transaction duration between merchants and customers. In general, an order from a customer may contain several items and these items need to be retrieved from the respective shelves of the warehouse before the packaging process can be started. The retrieval delay of an order can then be defined as the duration from the time when the order being input to the system until the time when the last item of the order is retrieved. To reduce the retrieval delay of orders, we combine the integrality of order with the job scheduling of ARS. We introduce the concept of Order Tag and propose two Order-Based scheduling algorithms according to the greedy strategy to reduce the total retrieval delay of orders. Mathematical models are constructed to classify and evaluate the retrieval problems. Both algorithms take polynomial time and the ideal state is that all the retrieval jobs are evenly distributed to stackers. Simulation results demonstrate that these two strategies can reduce the total retrieval delay by approximately 30% comparing to the existing algorithms.
Inactivation mechanisms of non-thermal plasma on microbes: A review
Liao, Xinyu,Liu, Donghong,Xiang, Qisen,Ahn, Juhee,Chen, Shiguo,Ye, Xingqian,Ding, Tian Elsevier 2017 Food Control Vol.75 No.-
<P>The increasing consumption of fresh-like food products requires the development of mild processing technologies without loss of nutritional value and sensory quality of foods. Non-thermal plasma (NTP) is an emerging and promising technology for extending the shelf-life of food products. However, the further development of a novel preservation technology should base on the adequate understandings of the effects on microbial behaviors. Therefore, the aim of this review is to provide an overview of the inactivation mechanisms of NTP technology on microbes. Topics covered are the basic introduction of NTP, the intrinsic and extrinsic factors affecting microbial inactivation effect and the probable mechanisms for microbial inactivation. Many factors, including processing parameters, environmental conditions and microbial properties have been shown to influence the bactericidal effect of NTP. According to previous research, the inhibitory activity of NTP against microbes includes biological and physical scenarios, though the exact mechanisms still remain unknown, requiring more investigations in the future. (C) 2016 Elsevier Ltd. All rights reserved.</P>
Selective hydrogenation of benzene over Ru supported on surface modified TiO2
Fang Hao,Jingsong Zheng,Donghong Ouyang,Wei Xiong,Pingle Liu,Hean Luo 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.4
A series of catalysts with ruthenium nanoparticles (NPs) loaded on the binary oxide TS (TiO2-SiO2) support were prepared by facile sol-gel method for benzene selective hydrogenation. Different mole ratios of Ti/Si were investigated. The catalytic structure, composition, hydrophilicity, electronic state and acidity were characterized. The results indicate that the acidity of the samples increases gradually with the increase of the SiO2 amount. The dispersion of ruthenium nanoparticles was improved on binary oxide support compared with TiO2 and an obvious Ru size effect appeared with the increase of Ti/Si molar ratio. It was also found that the hydrophilicity of the supports could be efficiently improved by introducing a small amount of SiO2 to TiO2, which may be due to the large amount of surface hydroxyl groups on the binary oxide samples after SiO2 introduction. This result was consistent with the increasing trend of cyclohexene yield, indicating that the surface hydroxyl groups play a significant role in the motivation of cyclohexene desorption. Ru/TS (7 : 1) showed the best catalytic performance of 38.2% yield to cyclohexene with the highest initial selectivity to cyclohexene (S0) of 87.3%. The catalytic stability experiments showed that the yield of cyclohexene could maintain at 30% after five cycles.
Liao, Xinyu,Li, Jiao,Suo, Yuanjie,Ahn, Juhee,Liu, Donghong,Chen, Shiguo,Hu, Yaqin,Ye, Xingqian,Ding, Tian Elsevier 2018 Food Research International Vol.105 No.-
<P>As the development of hurdle technology, cross-protection of various stresses for pathogens posed the potential risk to food safety and public health. This study tried to explore various preliminary stresses including acidity, osmosis, oxidation, heat and cold on the resistance of microbial cells toward the non-thermal plasma (NTP) exposure. The results indicated that short-term (4 h) exposure of Staphylococcus aureus and Escherichia coli to acidity, osmosis, oxidation, heat and cold stresses did not lead to the resistance to the subsequent NTP treatment. On the contrary, acidity, osmosis and heat preadaptation increased the vulnerability of E. coil cells to NTP treatment. After exposing S. aureus to osmosis, oxidation, heat and cold stress for longer period (24 h), the reduction level showed significantly (P < 0.05) higher. Interestingly, long-term (24 h) preliminary exposure of acidic stress exhibited protective effect for S. aureus against the following NTP exposure with less damage in cell membrane integrity, membrane potential and intracellular enzyme activity. It might be due to the protein production for oxidative stress response during preliminary acidic adaptation. In general, the obtained result helped to grasp better understanding of the microbial stress response to NTP treatment and provided insight for the future research in order to accelerate the development of NTP technology in food industry.</P>
Significance of Viable but Nonculturable Escherichia coli: Induction, Detection, and Control
( Tian Ding ),( Yuanjie Suo ),( Qisen Xiang ),( Xihong Zhao ),( Shiguo Chen ),( Xingqian Ye ),( Donghong Liu ) 한국미생물 · 생명공학회 2017 Journal of microbiology and biotechnology Vol.27 No.3
Diseases caused by foodborne or waterborne pathogens are emerging. Many pathogens can enter into the viable but nonculturable (VBNC) state, which is a survival strategy when exposed to harsh environmental stresses. Pathogens in the VBNC state have the ability to evade conventional microbiological detection methods, posing a significant and potential health risk. Therefore, controlling VBNC bacteria in food processing and the environment is of great importance. As the typical one of the gram-negatives, Escherichia coli (E. coli) is a widespread foodborne and waterborne pathogenic bacterium and is able to enter into a VBNC state in extreme conditions (similar to the other gram-negative bacteria), including inducing factors and resuscitation stimulus. VBNC E. coli has the ability to recover both culturability and pathogenicity, which may bring potential health risk. This review describes the concrete factors (nonthermal treatment, chemical agents, and environmental factors) that induce E. coli into the VBNC state, the condition or stimulus required for resuscitation of VBNC E. coli, and the methods for detecting VBNC E. coli. Furthermore, the mechanism of genes and proteins involved in the VBNC E. coli is also discussed in this review.
Hu, Lingping,Hu, Zhiheng,Chin, Yaoxian,Yu, Haixia,Xu, Jianhong,Zhou, Jianwei,Liu, Donghong,Kang, Mengli,Hu, Yaqin The Korean Society of Fisheries and Aquatic Scienc 2022 Fisheries and Aquatic Sciences Vol.25 No.3
A method of ultrahigh performance liquid chromatography coupled to high resolution mass spectrometry (UPLC-HRMS) was established for characterization of the lipid profile of Skipjack tuna. Over 300 lipid molecular species were identified through cross-acquisition in both positive and negative ion mode. Phospholipids (PLs) were dominant in Skipjack tuna. Lysophosphatidylethanolamine (LPE), phosphatidylethanolamine (PE), lysophosphatidylcholine (LPC) and phosphatidylcholine (PC) were the main lipid molecular species in PLs, accounting for 89.24% of the total PLs. The ratio of sphingolipids (SLs) and glycerolipids (GLs) were considerable, accounting for 12.30% and 13.60% of the total lipids respectively. Ceramide (Cer) was the main lipid molecular species of SLs, accounting for 64.96% of total SLs, followed by sphingomyelin (SM), accounting for 25.45% of total SLs. Ether diglycerides (ether DG) were the main lipid molecular species of GLs (97.83%). The main fatty acids (FAs) are unsaturated fatty acids (UFAs) in Skipjack tuna. Besides, a new FAs class branched fatty acid esters of hydroxy fatty acids (FAHFA) was detected, together with the FA. The active lipids identified in this study can be used to evaluate the nutritional value of Skipjack tuna.