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Synthesis and Characterization of Nanosized MnxFe2O4 Powders by Glycothermal Process
Dong-SikBae,Eun-JungKim,Hae-WonLee,Kyong-SopHan 한국세라믹학회 2002 한국세라믹학회지 Vol.39 No.10
Nanosized MnxFe2O4 powders were prepared in ethylene glycol solution under mild temperature and pressure conditions by precipitation from metal nitrates with aqueous potassium hydroxide. The average size and distribution of the synthesized MnxFe2O4 powders was about 20 ㎚ and broad, respectively. The phase of synthesized particles was crystalline reacted at 200 0C for 6 h.. The magnetic properties of the synthesized MnxFe2O4 powders were about 35-60 (emu/g) with superparamagnetic character.
Characterization of Cell Wall Proteins from the soo1-1/ret1-1 Mutant of Saccharomyces cerevisiae
Dong-WonLee,Ki-HyunKim,Se-ChulChun,Hee-MoonPark 한국미생물학회 2002 The journal of microbiology Vol.40 No.3
In order to investigate the function of Soo1p/α-COP during post-translational modification and intracellular transport of cell wall proteins in Saccharomyces cerevisiae, cell wall proteins from the soo1-1/ ret1-1 mutant cells were analyzed. SDS-PAGE analysis of biotin labeled cell wall proteins suggested that the soo1-1 mutation impairs post-translational modification of cell wall proteins, such as N- and/ or O-glycosylation. Analysis of cell wall proteins with antibodies against β-1,3-glucan and β-1,6-glucan revealed alteration of the linkage between cell wall proteins and β-glucans in the soo1-1 mutant cells. Compositional sugar analysis of the cell wall proteins also suggested that the soo1-1 mutation impairs glycosylation of cell wall protein in the ER, which is crucial for the maintenance of cell wall integrity.
A New Phenylpropanoid Glucoside from the Fruits of Illicium verum
Sung-WonLee,GaoLi,Kyong-SunLee,Dong-KeunSong,Jong-KeunSon 대한약학회 2003 Archives of Pharmacal Research Vol.26 No.8
One new phenylpropanoid glucoside (3), along with one known phenylpropanoid (1), and one known alkyl glucoside (2) were isolated from the fruits of Illicium verum and their chemical structures were elucidated on the basis of spectroscopic studies.
HwanYoun,JangyulKwak,Dong-WonKim,Chang-JinLee,Yang-InYim,Jin-WonLee,In-KwonKim,Jeong-IlYu,임형순,Sa-OukKang 한국미생물학회 2002 The journal of microbiology Vol.40 No.1
A cluster of genes encoding the pyruvate dehydrogenase complex (PDC) of Streptomyces seoulensis, a Gram-positive bacterium, was cloned and sequenced. The genes of S. seoulensis consist of four open reading frames. The first gene, lpd, which encodes a lipoamide dehydrogenase, is followed by pdhB encoding a dihydrolipoamide acetyltransferase (E2p), pdhR, a regulatory gene, and pdhA encoding a pyruvate dehydrogenase component (E1p). E1p had an unusual homodimeric subunit, which has been known only in Gram-negative bacteria. S. seoulensis E2p contains two lipoyl domains like those of humans and Streptococcus faecalis. The pdhR gene appears to be clustered with the structural genes of S. seoulensis PDC. The PdhR-overexpressed S. seoulensis showed growth retardation and the decrease of E1p, indicating that PdhR regulates the function of PDC by repressing the expression of E1p. A strain of Streptomyces lividans overexpressing S. seoulensis PdhR showed a significant decrease in the level of actinorhodin, implying a regulatory role for Streptomyces PDC in antibiotic biosynthesis.
A Simulation Study for the Logistics Planning of a Container Operations in view of SCM
Tae-WooLee,Nam-KyuPark,Dong-WonLee 한국항해항만학회 2002 한국항해항만학회지 Vol.26 No.6
This paper aims to apply a supply chain modeling and its analysis framework to the supply chain in the port industry. The simulation approach serves two purposes: to model a supply chain network in quantity approach and to evaluate its supply chain performance based on proposed strategies. Through the modeling works to improve the performance, the components of simulation model such as input model, strategy model, operational policy model and performance model in port supply chain were identified. The effects of various strategies can guide the way to administrate the supply chain in the different objectives.