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Dong, Yong-Qiang,Liang, Jiang-Shui,Zhu, Shui-Bo,Zhang, Xiao-Ming,Ji, Tao,Xu, Jia-Hang,Yin, Gui-Lin Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7
Objective: The present study employed 5-aza-2'-deoxycytidine (5-Aza-CdR) to treat non-small cell lung cancer (NSCLC) cell line A549 to investigate the effects on proliferation and expression of the TFPI-2 gene. Methods: Proliferation was assessed by MTT assay after A549 cells were treated with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR, a specific demethylating agent, for 24, 48 and 72h. At the last time point cells were also analyzed by flow cytometry (FCM) to identify any change in their cell cycle profiles. Methylation-specific polymerase chain reaction (MSPCR), real time polymerase chain reaction(real-time PCR) and western blotting were carried out to determine TFPI-2 gene methylation status, mRNA expression and protein expression. Results: MTT assay showed that the growth of A549 cells which were treated with 5-Aza-CdR was significantly suppressed as compared with the control group (0 ${\mu}mol/L$ 5-Aza-CdR). After treatment with 0, 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h, FCM showed their proportion in G0/G1 was $69.7{\pm}0.99%$, $76.1{\pm}0.83%$, $83.8{\pm}0.35%$, $95.5{\pm}0.55%$ respectively (P<0.05), and the proportion in S was $29.8{\pm}0.43%$, $23.7{\pm}0.96%$, $15.7{\pm}0.75%$, $1.73{\pm}0.45%$, respectively (P<0.05), suggesting 5-Aza-CdR treatment induced G0/G1 phase arrest. MSPCR showed that hypermethylation in the promoter region of TFPI-2 gene was detected in control group (0 ${\mu}mol/L$ 5-Aza-CdR), and demethylation appeared after treatment with 1, 5, 10 ${\mu}mol/L$ 5-Aza-CdR for 72h. Real-time PCR showed that the expression levels of TFPI-2 gene mRNA were $1{\pm}0$, $1.49{\pm}0.14$, $1.86{\pm}0.09$ and $5.80{\pm}0.15$ (P<0.05) respectively. Western blotting analysis showed the relative expression levels of TFPI-2 protein were $0.12{\pm}0.01$, $0.23{\pm}0.02$, $0.31{\pm}0.02$, $0.62{\pm}0.03$ (P<0.05). TFPI-2 protein expression in A549 cells was gradually increased significantly with increase in the 5-Aza-CdR concentration. Conclusions: TFPI-2 gene promoter methylation results in the loss of TFPI-2 mRNA and protein expression in the non-small cell lung cancer cell line A549, and 5-Aza-CdR treatment could induce the demethylation of TFPI-2 gene promoter and restore TFPI-2 gene expression. These findings provide theoretic evidence for clinical treatment of advanced non-small cell lung cancer with the demethylation agent 5-Aza-CdR. TFPI-2 may be one molecular marker for effective treatment of advanced non-small cell lung cancer with 5-Aza-CdR.
Dong-Bo Sheng,Sang-Bong Kim,Trong-Hai Nguyen,Dae-Hwan Kim,Tian-Shui Gao,Hak-Kyeong Kim 한국동력기계공학회 2016 동력시스템공학회지 Vol.20 No.4
This paper proposes two measurement methods for injured rate of fish surface using color image segmentation method based on K-means clustering algorithm and Otsu’s threshold algorithm. To do this task, the following steps are done. Firstly, an RGB color image of the fish is obtained by the CCD color camera and then converted from RGB to HSI. Secondly, the S channel is extracted from HSI color space. Thirdly, by applying the K-means clustering algorithm to the HSI color space and applying the Otsu’s threshold algorithm to the S channel of HSI color space, the binary images are obtained. Fourthly, morphological processes such as dilation and erosion, etc. are applied to the binary image. Fifthly, to count the number of pixels, the connected-component labeling is adopted and the defined injured rate is gotten by calculating the pixels on the labeled images. Finally, to compare the performances of the proposed two measurement methods based on the K-means clustering algorithm and the Otsu’s threshold algorithm, the edge detection of the final binary image after morphological processing is done and matched with the gray image of the original RGB image obtained by CCD camera. The results show that the detected edge of injured part by the K-means clustering algorithm is more close to real injured edge than that by the Otsu’ threshold algorithm.
Biphasic effects of TGFβ1 on BMP9-induced osteogenic differentiation of mesenchymal stem cells
( Rui Dong Li ),( Zhong Liang Deng ),( Ning Hu ),( Xi Liang ),( Bo Liu ),( Jin Yong Luo ),( Liang Chen ),( Liang Jun Yin ),( Xiao Ji Luo ),( Wei Shui ),( Tong Chuan He ),( Wei Huang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2012 BMB Reports Vol.45 No.9
We have found that the previously uncharacterized bone morphogenetic protein-9 (BMP9) is one of the most osteogenic factors. However, it is unclear if BMP9 cross-talks with TGFβ1 during osteogenic differentiation. Using the recombinant BMP9 adenovirus, we find that low concentration of rhTGFβ1 synergistically induces alkaline phosphatase activity in BMP9-transduced C3H10T1/2 cells and produces more pronounced matrix mineralization. However, higher concentrations of TGFβ1 inhibit BMP9-induced osteogenic activity. Real-time PCR and Western blotting indicate that BMP9 in combination with low dose of TGFβ1 potentiates the expression of later osteogenic markers osteopontin, osteocalcin and collagen type 1 (COL1a2), while higher concentrations of TGFβ1 decrease the expression of osteopontin and osteocalcin but not COL1a2. Cell cycle analysis reveals that TGFβ1 inhibits C3H10T1/2 proliferation in BMP9-induced osteogenesis and restricts the cells in G0/G1 phase. Our findings strongly suggest that TGFβ1 may exert a biphasic effect on BMP9-induced osteogenic differentiation of mesenchymal stem cells. [BMB Reports 2012; 45(9): 509-514]
THEORETICAL AND EXPERIMENTAL STUDY ON FLOW CHARACTERISTICS OF A DIAPHRAGM PUMP FOR UREA-SCR SYSTEMS
Shu Dong Yang,You Cheng Shi,Xi Wei Pan,Yin Shui Liu 한국자동차공학회 2019 International journal of automotive technology Vol.20 No.4
Urea selective catalytic reduction (SCR) is the primary technology used to reduce the nitrogen oxides (NOx) of diesel engine exhaust. To meet the requirements of an SCR system, a novel type of miniature diaphragm pump was designed. Based on the theory of large deflection of annular plates, the equilibrium equations of a diaphragm with a rigid inclusion were established, and the equations were solved by the nondimensional method and the finite difference method. Theoretical and approximated flow model for this pump were proposed. A theoretical relationship between back pressure, rigid inclusion size and volumetric efficiencies were calculated. To verify the validity of theoretical model, a prototype pump was fabricated and tested. Experimental results demonstrated that the flow is proportional to the pump speed. The deviation between theoretical, approximated flow and experimental flow was less than 4 % and 9.4 %, respectively. The difference between theoretical and experimental volumetric efficiency was varied from 2.7 % to 6.1 % when back pressure changed from 0 to 0.9 MPa. The volumetric efficiency was growing with the increasing of the rigid inclusion size. The pressures in the working chamber showed almost the same overall trends between the theoretical results and experimental values. The experimental results show that the proposed theoretical model is effective.
( Jing Han ),( Dong Sheng Wang ),( Shui Bing Liu ),( Ming Gao Zhao ) 한국응용약물학회 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.3
Cytisine (CYT), a partial agonist of α4β2-nicotinic receptors, has been used for antidepressant efficacy in several tests. Nicotinic receptors have been shown to be closely associated with depression. However, little is known about the effects of CYT on the depression. In the present study, a mouse model of depression, the unpredictable chronic mild stress (UCMS), was used to evaluate the activities of CYT. UCMS caused significant depression-like behaviors, as shown by the decrease of total distances in open field test, and the prolonged duration of immobility in tail suspension test and forced swimming test. Treatment with CYT for two weeks notably relieved the depression-like behaviors in the UCMS mice. Next, proteins related to depressive disorder in the brain region of hippocampus and amygdala were analyzed to elucidate the underlying mechanisms of CYT. CYT significantly reversed the decreases of 5-HT1A, BDNF, and mTOR levels in the hippocampus and amygdala. These results imply that CYT may act as a potential anti-depressant in the animals under chronic stress.
( Zhou Zhou ),( Dong Hai Peng ),( Jin Shui Zheng ),( Gang Guo ),( Long Jun Tian ),( Zi Niu Yu ),( Ming Sun ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5
We screened four B. thuringiensis strains whose parasporal inclusions contained the S-layer protein (SLP), and cloned two slp genes from each strain. Phylogenetic analysis indicated these SLPs could be divided into two groups, SLP1s and SLP2s. To confirm whether SLPs were present in the S-layer or as a parasporal inclusion, strains CTC and BMB1152 were chosen for further study. Western blots with isolated S-layer proteins from strains CTC and BMB1152 in the vegetative phase showed that SLP1s and SLP2s were constituents of the S-layer. Immunofluorescence utilizing spore-inclusion mixtures of strains CTC and BMB1152 in the sporulation phase showed that SLP1s and SLP2s were also constituents of parasporal inclusions. When heterogeneously expressed in the crystal negative strain BMB171, four SLPs from strains CTC and BMB1152 could also form parasporal inclusions. This temporal and spatial expression is not an occasional phenomenon but ubiquitous in B. thuringiensis strains. [BMB reports 2011; 44(5): 323-328]
Han, Jing,Wang, Dong-sheng,Liu, Shui-bing,Zhao, Ming-gao The Korean Society of Applied Pharmacology 2016 Biomolecules & Therapeutics(구 응용약물학회지) Vol.24 No.3
Cytisine (CYT), a partial agonist of ${\alpha}4{\beta}2-nicotinic$ receptors, has been used for antidepressant efficacy in several tests. Nicotinic receptors have been shown to be closely associated with depression. However, little is known about the effects of CYT on the depression. In the present study, a mouse model of depression, the unpredictable chronic mild stress (UCMS), was used to evaluate the activities of CYT. UCMS caused significant depression-like behaviors, as shown by the decrease of total distances in open field test, and the prolonged duration of immobility in tail suspension test and forced swimming test. Treatment with CYT for two weeks notably relieved the depression-like behaviors in the UCMS mice. Next, proteins related to depressive disorder in the brain region of hippocampus and amygdala were analyzed to elucidate the underlying mechanisms of CYT. CYT significantly reversed the decreases of 5-HT1A, BDNF, and mTOR levels in the hippocampus and amygdala. These results imply that CYT may act as a potential anti-depressant in the animals under chronic stress.
Physicochemical and Sensory Characterization of Ginger-juice Yogurt during Fermentation
Guo-Hao Yang,Jun-Jun Guan,Jin-Shui Wang,Haicheng Yin,Fa-Dong Qiao,Feng Jia 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
The primary purpose of this study was to investigate the effects of a ginger (Zingiber ofcinale) juice addition to milk on the physicochemical and sensory properties and consumer acceptability of yogurt during fermentation. Under the presence of ginger juice at 2.0 to 10.0%(v/v), there was a significant influence (p<0.05) on the viability of characteristic microorganisms in yogurt,the pH, titratable acidity (ºSH), viscosity, and hardness of the products. The descriptive data showed significant differences (p<0.05) among the samples for all attributes. The consumer results from external preference mapping indicated that samples with the lower ginger juice (≤4%)were liked over the higher samples (≥6%), with a negative impact on the acceptance by the consumers. Therefore,these findings suggest that 4%(v/v) may be the more appropriate content of ginger juice in the preparation of ginger-juice yogurt.