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DNA damage in T- and B-lymphocytes of rats exposed to benzene
Sul, Dong-Geun,Lee, Do-Young,Jo, Gyu-Chan,Im, Ho-Sub,Hong, Hyun-Ho,Jo, Duk-Jin,Kim, Chan-Wha,Kim, Hae-Joon,Lee, Eun-Il Korean Environmental Mutagen Society 2002 한국환경성돌연변이·발암원학회지 Vol.22 No.4
Single cell gel electrophoresis assay was carried out to evaluate DNA damage in T-and B-lymphocytes from rats exposed to benzene and the correlation between DNA damage and the level of t,t-muconic acids, which are urinary benzene metabolites, was investigated. In control rats, the mean values of Olive tail moments in T-and B-lymphocytes were 1.507$\pm$0.187 and 1.579$\pm$0.206 respectively. DNA damages of T-lymphocytes in rats exposed for 4 weeks showed the highest Olive tail moments at each benzene concentration examined (2.72-4.351). However this DNA damage was decreased after 6 weeks of exposure (1.74-2.09). DNA damages of B-lymphocytes did not show such differences with exposure time or benzene concentration (1.49-2.07) except at 200 ppm at 4 weeks. T-lymphocytes show significantly more damages than B-lymphocyte upon acute exposure to benzene.
( Young Joon Park ),( Uy Dong Sohn ),( Ji Hoon Jeong ),( Yoon Hee Chung ),( Sung Up Choi ),( Moo Yeol Lee ),( Eon Sub Park ),( Hyun Dong Je ),( Yun Sung Nam ),( Choong Ho Lee ),( Yong Sul Park ),( Zoo 한국응용약물학회 2011 Biomolecules & Therapeutics(구 응용약물학회지) Vol.19 No.1
The present study was undertaken to determine whether combined treatment with prokinetic trimebutine and mosapride has a synergic effect on gastrointestinal motility and visceral pain associated with gastrointestinal dysfunction. To develop effective gastroprokinetic agents with greater potencies than trimebutine or mosapride for the treatment of gastrointestinal tract disease, a mixture of trimebutine and mosapride was designed and prepared. In the present study, treatment with trimebutine alone showed a dose-dependent effect on propelling movements of normal small and large intestine in mice, whereas mosapride effected only small intestine motility. Co-administration of trimebutine with mosapride, a well-established prokinetic drug, produced a synergistic influence on normal small intestine motility, but demonstrated an unclear effect on large intestine motility, with a slight tendency to reduce the propelling time. In a stress model, the small and large intestine motilities were significantly decreased. The reduction of intestine motility was restored to a normal level and the restoring effect was more pronounced in the combined treatment with trimebutine plus mosapride than treatment with trimebutine or mosapride alone. Furthermore, treatment with trimebutine plus mosapride significantly decreased acute visceral pain which was not controlled by trimebutine or mosapride alone. These data suggest that combination therapy with trimebutine plus mosapride has a synergic effect on small and large intestine motility and visceral pain control in gastrointestinal disorders
Effects of Polycyclic Aromatic Hydrocarbons on DNA Damage and Plasma Protein Expression in Mouse
Oh, Sang-Nam,Oh, Eun-Ha,Im, Ho-Sub,Jo, Gyu-Chan,Sul, Dong-Geun,Kim, Young-Whan,Lee, Eun-Il The Korean Society of Toxicogenomics and Toxicopro 2005 Molecular & cellular toxicology Vol.1 No.1
Polycyclic aromatic hydrocarbons (PAHs) are an important class of environmentally prevalent xenobiotics that exert complex effects on the biological system and characterized as probably carcinogenic materials. Single cell gel electrophoresis assays were performed in order to evaluate DNA damage occurring in the T-and B lymphocytes, spleens (T/B-cell), bone marrow, and livers of mouse exposed to mixture of PAHs (Benzo(a)pyrene, Benzo(e)pyrene, Fluoranthene, Pyrene) at dose of 400, 800, or 1600 mg/kg body weight for 2 days. DNA damage of the cells purified from mice was increased in dose dependent manner. In the blood cells and organs, DNA damage was also discovered to vary directly with PAHs. Especially T-cells had been damaged more than B-cell. Plasma proteomes were separated by 2-dimensional electrophoresis with pH 4-7 ranges of IPG Dry strips and many proteins showed significant up-and -down expressions with the dose dependent manner. Of these, significant 4 spots were identified using matrix-assisted laser desorption/ionization-time of fight (MALDI-TOF) mass spectrometry. Identified proteins were related to energy metabolism and signal transduction.
소 성장호르몬 cDNA 및 유전자의 분리와 대장균에서 cDNA 의 발현
김연수,정신우,김병한,이명희,설동섭,강현삼 생화학분자생물학회 1992 BMB Reports Vol.14 No.2
cDNA was prepared from poly(A)^+mRNA isolated from pituitaries and cloned in E. coli. It contains the coding sequence for bovine growth hormone(bGH), The cloned bGH cDNA was confirmed by colony hybridization, restriction enzyme mapping, and nucleotide sequencing. The cloned eDNA was modified to construct the plasmid that expresses the bovine growth hormone in bacteria. A genomic gene of bovine growth hormone was also cloned using the bGH cDNA as a probe.