괄루근의 Glycosidase들에 대한 연구

류병호,양승택,조은정,김동수,문윤희,김희숙 慶星大學校 1996 論文集 Vol.17 No.2

Several glycosidase activities, α-galactosidase, β-galactosidase and β-fucosidase and their isozymes were founded in Trichosantes kirilowii root, Trichosantes radix. The activities of α-glucosidase, β-glucosidase and α-fucosidase were also assayed but were not detected. Those glycosidases were isolated and partial purified glycosidases by acid-treated Sepharose-6B, anion exchange Mono-Q HPLC, DEAE-Sephacel, butyl-toyopearl hydrophobic column chromatographies. However, these glycosidases need to be purified more homogeneous and characterized.

복합레진 수복시 복합용기 및 단일용기 상아질 접착제의 미세변연누출에 관한 연구

류주희,박동성,권혁춘 大韓齒科保存學會 1999 Restorative Dentistry & Endodontics Vol.24 No.1

N/O/N 박막 다이아프레임을 이용한 광섬유 압력센서의 제작 및 그 압력특성

유양욱,김명규,박동수,김창원,김진섭,이정희,손병기 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

The 0.6 μm N/O/N triple layer, Si_(3)N_(4)(150 nm)/SiO_(2)(300 nm)/Si_(3)N_(4)(150 nm), was deposited on silicon substrate and the N/O/N thin film diaphragm was formed using anisotropic etching technique. The stress of the formed diaphragm was very small with temperature variations. Combining this diaphragm and the optical fiber, intensity-type pressure sensor was fabricated and its pressure characteristics were investigated. The relation between the optical output power, inversely proportional to the deflection of the diaphragm, and the applied pressure was almost linear in 0~77 torr range.

대두단백질 가수분해물의 특성

김동수,양승택,문윤희,류병호,김희숙,염동민,김진목 慶星大學校 1998 論文集 Vol.19 No.1

The present study was conducted to elucidate some characteristics of soy protein hydrolysates. The soy protein was obtained from defatted soy power by the treatment of n-hexan. The hydrolysates of soy protein were prepared from hydrolysis by bromelian and pepsin with the two kinds of samples of precooking for 30 min in steam oven and noncooking, and fractionated through Bio-gel P-4 column chromatography. Fractions of hydrolysates separated on the Bio-gel column chromatography were carried out to SDS-PAG electrophoresis, resulting to show some different bends from 66kD to 14.2kD of molecular weight. In addition, the fractions showed different activities of superoxide dismutase. From these results, it was found that the hydrolysates could be available to use in a lot of food industrial fields as new functional food materials because the hydrolysates had different characteristics in electrophoresis and could increase the activity of superoxide dismutase.

완전 포상기태 이후 발생한 지속성 융모질환의 치료경험 2례.

이진희,류은석,김윤숙,김종수,최승도,선우재근,배동한 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Persistent tumor develops in approximately 20% of patients with a complete mole, and chemotherapy is required to achieve remission. Following evacuation of hydatidiform mole, careful hCG monitoring is mandatory since it is the most reliable and sensitive method for the early detection of gestational trophoblastic disease. In carefully selected patients in whom the risk of developing gestational trophoblastic disease is significant or when the availability of hCG testing is suboptimal, chemoprophylaxis has been shown to decrease the risk of gestational trophoblastic tumor. We report here two cases of patient, 16-year-old and 45-year-old women who experienced unusual course after the evacuation of a complete mole and markedly elevated serum β-hCG levels. The patient developed persistent gestational trophoblastic disease and were successfully treated with EMA-CO and hysterectomy.

반코마이신 내성 장내구균 분리 환자에서 황색포도알균의 내성

김동욱,신선혜,김혜진,류선,이선희,장철훈,김영대,곽임수 대한감염학회 2004 감염과 화학요법 Vol.36 No.1

목적 : Vancomycin-resistant enterococci (VRE)로부터 S. aureus로의 vacomycin 내성 유전자의 전파는 오래전부터 예견되어져 왔으며 큰 걱정거리로 남아있다. 최근에 vanA 유전자를 가지고 있는 vancomycin-resistant S. aureus 2주가 미국에서 분리되었다. 이 연구의 목적은 VRE를 보균하고 있는 환자들에서 S. aureus 보균양상과 항균제 내성을 알아보는데 그 목적이 있다. 재료 및 방법 : 2001년 1월부터 2001년 12월까지 부산대학교병원에 입원하였던 환자들 중 임상검체에서 VRE가 분리된 환자 7명과 2001년 9월부터 12월까지 외과 중 환자실에 입원하였던 환자들 중 VRE 감시배양을 시행하였던 20명의 환자들을 대상으로 하였다. 환자들은 전비공, 액와부, 회음부, 직장에서 매주 면봉으로 검체를 채취하였다. 선별배지에서 검체를 배양하여 S. aureus를 분리하였고 원판 확산법을 이용하여 methicillin 감수성을 측정하였다. Broth microdilution 법으로 vancomycin MIC를 측정하였다. 결과 : 총 27명에서 73회의 방문과 292회의 배양이 시행되었으며 총 67주의 S. aureus가 분리되었다. 이중 64주(95.5%)가 methicillin 내성이었다. 전비공 MRSA 보균율은 VRE를 보균하고 있었던 환자는 19명 중 11명(58%)에서, VRE를 보균하지 않았던 환자는 8명 중 3명(37.5%)으로 VRE 보균자에서 보균율이 더 높았다. 총 64주의 MRSA의 vancomycin MIC의 분포는 0.5-2㎍/mL였으며 2㎍/mL를 초과하는 균주는 발견되지 않았다. 64주 중 vancomycin MIC가 1㎍/mL인 균주가 54주(84.4%)로 가장 많았으며 2㎍/mL 6주(9.4%), 0.5㎍/mL 4주(6.3%)의 순이었다. 결론 : VRE 환자에서 S. aureus의 보균율은 비보균자 보다 높았으며 분리된 균주들은 대부분 MRSA였다. VRSA 균주는 발견되지 않았다. Background : The transfer of vancomycin resistance from vancomycin-resistant enterococci (VRE) to Staphylococcus aureus has been predicted. The purpose of this study is to determine the prevalence and antibiotics resistance of S. aureus among patients colonized with VRE. Methods : Between January 2001 and December 2001, a prospective study was performed at Pusan National University Hospital on 27 patients. Surveillance swabs from nasal cavity, axilla, perineum, and rectum were obtained at weekly intervals. Methicillin susceptability of S. aureus was determined by oxacillin disk diffusion test and minimum inhibitory concentration (MIC) for vancomycin by microdilution broth test. Results : Total of 292 swab cultures were performed and 67 S. aureus isolates were collected. 64 isolates (95.5%) were resistant to methicillin. The prevalence of nasal MRSA carrier in 19 patients colonized with VRE was higher than that in 8 patients not colonized with VRE (58% vs. 37.5%). In 64 MRSA isolates, MIC (㎍/mL) for vancomycin ranged from 0.5 to 2. No isolates with MIC >2 ㎍/mL were observed. MIC of 1 ㎍/mL was shown (observed) in 54 isolates, 2 ㎍/mL in 6 isolates, and 0.5 ㎍/mL in 4 isolates. Conclusion : The prevalence of S. aureus with colonization of VRE is higher than that without colonization of VRE. Most of S. aureus isolates were resistant to methicillin. VRSA isolates were not observed.

2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione(NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 한국환경독성학회 2000 환경독성보건학회지 Vol.15 No.4

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y 15 induced his+ revertants of Salmonella typhimurium TA 98 and TA1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his^(+) revertants of Salmonella typhimurium TA1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells, NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

고정층과 순환유동층에서 CaSO_4의 환원반응에 대한 온도와 CO농도의 영향

배달희,류호정,박재현,이창근,선도원,이동규 한국화학공학회 2003 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.41 No.1

순환유동층 석탄연소로에서 석회석에 의한 찰황반응에 영향을 미치는 CaSO_4의 환원반응에 의한 SO-2 재배출을 실험적으로 확인하고 SO_2의 재배출에 미치는 온도와 CO 농도의 영향에대해 상용 순환유동층에 적용할 수 있는 기초자료를 확보하기 위해 고정층과 순환유동층에서 층물질로 석회석과 상용 순롼 유동층 하부회를 이용하여 환원실험을 수행하였으며 CaSO_4의 환원반응에 미치는 온도와 CO의 영향을 측정 및 해석하였다. 고온조건에서 CO가 환원제로 작용하여 C_aSO_4로부터 SO_2가 재배출되는 현상을 확인하였으며 CaSO_4로부터 SO_2의 재배출은 온도와 CO농도가 증가함에 따라 증가하였다. 본 실험의 결과에 의해 온도가 증가함에 따라 석회석에 의한 탈황율이 감소하는 현상을 환원이론으로 설명할 수 있다는 것이 확인되었다. For qualitative understanding of the sulphur capture process in a circulating fluidized bed fumace, the effects of temperature and CO concentration on the reactivity of partially sulfated CaO were experimentally examined. The tests were performed in a fixed bed reactor and lab-scale circulating fluidized bed reactor. The materials used were partially sulfated domestic limestone and bottom ash drained from commercial circulating fluidized bed furnaces. The re-emission of SO_2 from partially sulfated limestone and bottom ash increases with temperature as well as with concentration of the reducing agent CO. From the results of this study, the temperature dependence of sulfation could be explained by reduction theory.

복어卵巢 鹽藏品 製造中의 化學的 成分 및 毒性의 變化

김동수,박지현,류병호,양승택,문윤희,김희숙 慶星大學校 1993 論文集 Vol.14 No.4

Attempts were made to investigate the levels of chemical components and detoxification of the salted puffer overies, Fugu xanthopterus, "Ggachibog" and Fugu rubripes rubripes, "Chambog", processing of solid salt mixtures containing sodium bicarbonate, during storage period. Fresh ovaries of the puffers were divided into three portions, sprinkled with a solid salt mixture containing 0,1 or 2% NaHCO₃, and were stored at room temperature for 12 weeks. The samples were examined at each step and assayed for pH, VBN, amino-N, toxicity and thin- layer chromatogram pattern. The results were summarized as follows: The values of pH were appeared to be different with the added amount of siodium bicarbonate and the values of pH were over 7.0 in a salt mixture containing 2% NaHCO₃. The change of VBN value was also revealed about 85mg/100g in A division and 90-100mg/100g in both B and C, respectively, in the salted ovary of puffer "Ggachibog" from 8 weeks. In case of salted product from the "Chamboh" ovary, it had slightly lower value than that of "Ggachibog" ovary. Also, the value of amino-N was increased until 4-6 weeks and was slowly down after the weeks in all samples. All salted products were found to lose most of the toxicity during storage period when the salt containing NaHcO₃was used for sprinkling. In the case of raw ovaries being weak toxicity below 5.0 MU/g or 24.1 MU/g, the toxicity of salted product was dropped near the level to be edible. Therefore there was significant detoxification of the puffer ovaries by the salt mixture containing sodium bicarbonate. Finally, the toxins isolated from each raw puffer ovary were detected to be TTX-related components by thin-layer chromatogrphy.

2-[(4-Cyanophenyl)amino]-3-chloro-1,4-naphthalenedione (NQ-Y15)의 돌연변이원성

김봉희,정기화,유충규,창동신,이기선,전선덕,소동수,채상호,문창규 德成女子大學校 藥學硏究所 2001 藥學論文誌 Vol.12 No.1

2-[(4-Cyanophenyl)amino]-3-chloro-1, 4-naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y15 induced his+revertants of Salmonella typhimurium TA 98 and TA 1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his+revertants of Salmonella typhimurium TA 1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells. NQ-Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.