Study on the Molecular Mechanism of Antioxidant Health Effect of Tuna Dark Meat Enzymatic Polypeptides

Diya Zhang East Asian Health Association 2024 Journal of East Asian Bealth Vol.1 No.1

Purpose – The purpose of this paper is to explore the molecular mechanism of polypeptide antioxidant health, and effectively promote the new direction of human health functions. Design/Methodology/Approach – Based on methodology, the paper studies this article uses trypsin to enzymatically hydrolyze tuna dark meat, obtained an enzymatic biological active polypeptide, and performs peptide sequencing using matrix-assisted laser dissociation time-of-flight mass spectrometry (MALDI TOF/TOF),obtained the first -level (MS) and second structure (MS/MS) of the peptide. Finally, the molecular docking was performed through Discovery Studio (DS), target protein prediction is performed to determine the connection with antioxidant health care. Findings – The results show that the dominant peptide sequences in the tuna dark meat trypsin hydrolyzed polypeptides are SSAR, VSSK and GEHAE; through molecular simulation methods (Discover Studio, DS), the potential target protein screening of the above enzyme polypeptide is found to be closely linked to Keap1 (oxidation) protein, so it is determined to have a certain connection with antioxidant molecules. Research Implications – In the management of this paper, In this article, this study is of great significance in antioxidant molecules. It provides a new direction for the removal of free radicals and effectively suppress the aging process. The health effect is significant, which will also provide a reference for the subsequent research of other scholars.

The effects of autonomy gap in personnel policy, principal leadership and teachers’ self-efficacy on their organizational commitment

Diya Dou,Geert Devos,Martin Valcke 서울대학교 교육연구소 2016 Asia Pacific Education Review Vol.17 No.2

School autonomy in personnel policy is important to effective personnel management. With increased autonomy in personnel policy, principals could wield their leadership to improve teachers’ organizational commitment. However, little is known about whether the given autonomy in personnel policy meets principals’ expectation and whether and how the gap in between has an effect on teachers’ organizational commitment. This research explores the effects of autonomy gap in personnel policy, principal leadership, teachers’ self-efficacy and school contextual factors on teachers’ organizational commitment. Multilevel analysis was used. The results show the effects of autonomy gap in personnel policy differ among areas. In particular, the autonomy gap in salary was found to be a significant antecedent of teachers’ organizational commitment. The results also highlight the importance of principal leadership in this process. Principals with a stronger leadership tend to perceive generally a larger autonomy gap in personnel policy. In addition, when transformational leadership increased, its positive influence on teachers’ organizational commitment became even stronger. Implications for developing efficacious leadership and autonomy policy in personnel management were discussed.

Hybrid of Fenton and sequencing batch reactor for petroleum refinery wastewater treatment

B.H. Diya’uddeen,Shima Rahim Pouran,A.R. Abdul Aziz,S.M. Nashwan,Wan Mohd Ashri Wan Daud,M.G. Shaaban 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.25 No.-

The treatment of high-strength petroleum refinery effluent (PRE) with [BOD5]/[COD]0 = 0.14 was investigated using combined Fenton–sequencing batch reactor (SBR). The effect of [H2O2]:[COD]0 molar ratio on biodegradability enhancement was investigated. Fenton optimal conditions were: [H2O2]:[COD]0 of 6, [H2O2]:[Fe0] of 10 and pH = 3.0 that could remove 76.5%, 45% and 96% of COD, TOC and phenol and increased biodegradability ratio to 0.37. The pretreated PRE subjected to SBR for one single cycle of 10 h. The results revealed the effectiveness of hybrid Fenton–SBR system in treating PRE where the complete treatment of the PRE was attained by this system.

Self-Assembled and Langmuir-Blodgett Arachidic Acid Monolayers on Silver: A Comparative Reflection-Absorption Fourier Transform Infrared Spectroscopic Study

Ahn, Sang-Jung,Mirzakhojaev, Diyas A.,Son, Dong-Hee,Kim, Kwan Korean Chemical Society 1994 Bulletin of the Korean Chemical Society Vol.15 No.5

Self-assembled (SA) and Langmuir-Blodgett (LB) monolayers of arachidic acid on silver surfaces have been investigated by a reflection-absorption Fourier transform infrared spectroscopy. Arachidic acid was adsorbed on silver as carboxylate with its two oxygen atoms bound symmetrically to the surface. Although both the SA and LB monolayers consisted of fully extended trans zigzag carbon chains, a closer examination indicated that the SA monotayers should possess a more ordered crystalline structure than the LB monolayers. The infrared spectral data dictated that the extent of methyl group exposure at the air-film interface was greater in the SA monolayers than the LB monolayers, in agreement with the contact angle measurement. From a theoretical analysis, the alkyl chains in each monolayers seemed to be tilted away from the surface normal by less than $3.5^{\circ}$, but in opposite directions. Arachidic acid monolayers were concluded to have same structure as stearic acid monolayers.

Does Low-Dose Heparin Have a Significant Role in Free Flap Surgery?

안문영,신진용,이영근,M. Diya Sabbagh,노시균,이내호 대한두개안면성형외과학회 2017 Archives of Craniofacial Surgery Vol.18 No.3

Background: It is controversial issue that heparin decreases thrombosis for microsurgical anastomosis, and its effective role is under discussion. This study is for proving whether low-dose heparin is preventing thrombosis in free flap reconstruction. Methods: Through chart reviews of 134 patients, using low-dose heparin for free tissue transfer from 2011 to 2016, retrospective analysis was performed. 33 patients received lowdose heparin therapy after surgery. And 101 patients received no-heparin therapy. Complications included flap necrosis, hematoma formation, dehiscence and infection. Results: In no-heparin therapy group, comparing the flap necrosis revealed 16 cases (15.84%). And, flap necrosis was 6 cases (18.18%) in low-dose heparin therapy group. The statistical analysis of flap necrosis rate showed no significant difference ( p =0.75). The results showed that there was no significant difference of flap necrosis rate between two groups. Conclusion: In this study, patients in the low-dose heparin group had no significantly lower rates of flap failure compared with no-heparin group. This suggests that low-dose heparin may not prevent thrombosis and subsequent flap failure to a significant extent.

Immunohistochemical Analysis of the Expression of Cytokeratins in Acquired Cholesteatoma and Its Clinico-Radiological Correlation

Kanodia Anupam,Kakkar Aanchal,Verma Yash,Roy Diya,Verma Hitesh,Singh Chirom Amit,Monga Rabia,Jain Deepali,Thakar Alok,Sikka Kapil 대한청각학회 2023 Journal of Audiology & Otology Vol.27 No.2

Background and Objectives: Cholesteatomatous chronic otitis media acquires epithelial proliferation and differentiation characteristics, which render it able to erode the underlying bone and cause complications. We attempt to characterize the cholesteatoma epithelium by observing the expression of cytokeratins (such as 34ße12, CK17, and CK13) and Ki67 among patients with cholesteatoma with different aggressiveness as compared to disease-free controls.Subjects and Methods: In this prospective study (2017-2021), we enrolled all consenting consecutive patients with cholesteatomatous chronic otitis media. They were staged in accordance with the staging guidelines of the European Academy of Otology and Neurotology and the Japanese Otological Society. Bony external auditory canal (EAC) skin specimens of the patients undergoing tympanoplasty were chosen as controls. We did an immunohistochemical analysis of the cholesteatoma specimens and normal bony EAC controls by observing the expression of 34ße12, CK17, CK13, and Ki67 across the layers of the epithelium. Fisher’s exact test and chi-square test were used to evaluate any statistical significance between the cases and the controls, and the subgroups were made based on the clinical stage.Results: An increased expression of CK17 (<i>p</i><0.001), CK13 (<i>p</i><0.03), and Ki67 (<i>p</i><0.001) was observed in cholesteatoma specimens when compared to normal bony EAC controls. Also, there was a loss of expression of 34ße12 in a subset of cholesteatoma specimens, all of which showed full-thickness expression of CK13. There was no difference in the expression of cytokeratin among specimens from patients belonging to different subgroups based on clinical stage, age, sex, duration of ear symptoms, or type of hearing loss (conductive vs. sensorineural).Conclusions: The majority of cholesteatoma specimens significantly overexpressed CK17, CK13, and Ki67 when compared to normal bony EAC skin controls, while a subset showed loss of expression of 34ße12, which provides some insight into its pathogenesis.

Factors affecting in vitro embryo production: insights into dromedary camel

Adel R. Moawad,Ibrahim M. Ghoneim,Gamal M. Darwish,Magdy R. Badr,Diya A. El-Badry,Abou Bakr A. EL-Wishy 사단법인 한국동물생명공학회 2020 한국동물생명공학회지 Vol.35 No.2

The Dromedary camel (Camelus dromedaries) is an important species because of its ability to produce good quality meat, milk, and fibers under harsh environmental conditions. Camels are also crucial for transportation, racing, and as draft animals in agriculture. Therefore, dromedary camels play a critical role in the economy for millions of people living in the arid part of the world. The inherent capability of camels to produce meat and milk is highly correlated with their reproductive performance. Compared with other domestic species, the reproductive efficiency in camelids is low. Although recent reproductive technologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) have been successfully applied to camelids and the birth of live offspring following these technologies has been reported; in vitro embryo production (IVP) has lagged in this species. The development of the IVP system for dromedary camels may be a useful tool for the genetic improvement of this species. IVP in farm animals includes three main steps; in vitro maturation (IVM) of an oocyte, IVF of a matured oocyte, and in vitro culture (IVC) of fertilized oocyte up to the blastocyst stage. This review aims to summarize various factors that influence oocyte quality, IVM, and in vitro embryo development in dromedary camel.

Factors affecting in vitro embryo production: insights into dromedary camel

Adel R. Moawad,Ibrahim M. Ghoneim,Gamal M. Darwish,Magdy R. Badr,Diya A. El-Badry,Abou Bakr A. EL-Wishy 한국동물생명공학회(구 한국동물번식학회) 2020 Journal of Animal Reproduction and Biotechnology Vol.35 No.2

The Dromedary camel (Camelus dromedaries) is an important species because of its ability to produce good quality meat, milk, and fibers under harsh environmental conditions. Camels are also crucial for transportation, racing, and as draft animals in agriculture. Therefore, dromedary camels play a critical role in the economy for millions of people living in the arid part of the world. The inherent capability of camels to produce meat and milk is highly correlated with their reproductive performance. Compared with other domestic species, the reproductive efficiency in camelids is low. Although recent reproductive technologies such as in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) have been successfully applied to camelids and the birth of live offspring following these technologies has been reported; in vitro embryo production (IVP) has lagged in this species. The development of the IVP system for dromedary camels may be a useful tool for the genetic improvement of this species. IVP in farm animals includes three main steps; in vitro maturation (IVM) of an oocyte, IVF of a matured oocyte, and in vitro culture (IVC) of fertilized oocyte up to the blastocyst stage. This review aims to summarize various factors that influence oocyte quality, IVM, and in vitro embryo development in dromedary camel.