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- 저자 : Diya Zhang (검색결과 1 건)
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Diya Zhang East Asian Health Association 2024 Journal of East Asian Bealth Vol.1 No.1
Purpose – The purpose of this paper is to explore the molecular mechanism of polypeptide antioxidant health, and effectively promote the new direction of human health functions. Design/Methodology/Approach – Based on methodology, the paper studies this article uses trypsin to enzymatically hydrolyze tuna dark meat, obtained an enzymatic biological active polypeptide, and performs peptide sequencing using matrix-assisted laser dissociation time-of-flight mass spectrometry (MALDI TOF/TOF),obtained the first -level (MS) and second structure (MS/MS) of the peptide. Finally, the molecular docking was performed through Discovery Studio (DS), target protein prediction is performed to determine the connection with antioxidant health care. Findings – The results show that the dominant peptide sequences in the tuna dark meat trypsin hydrolyzed polypeptides are SSAR, VSSK and GEHAE; through molecular simulation methods (Discover Studio, DS), the potential target protein screening of the above enzyme polypeptide is found to be closely linked to Keap1 (oxidation) protein, so it is determined to have a certain connection with antioxidant molecules. Research Implications – In the management of this paper, In this article, this study is of great significance in antioxidant molecules. It provides a new direction for the removal of free radicals and effectively suppress the aging process. The health effect is significant, which will also provide a reference for the subsequent research of other scholars.
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