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RISS 인기검색어
- 검색키워드
- 저자 : Dhama, P. (검색결과 4 건)
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Pathak, A.,Singh, P.,Dhama, P.,Dastidar, M.G.,Kim, D.J.,Heyes, G. Maney Publishing 2014 Canadian metallurgical quarterly Vol.53 No.1
The present study investigated the changes in nutrient profile of sewage sludge during bioleaching in a batch mode of operation. The study identified the optimum bioleaching period at which maximum solubilisation of metals is achieved while maintaining the fertilising property of the bioleached sludge. The bioleaching experiments were performed using anaerobically digested sewage sludge by employing indigenous iron and sulphur oxidising microorganisms. The results showed that bioleaching using sulphur oxidising microorganisms is comparatively advantageous due to the higher solubilisation of heavy metals. However, despite its high potential in solubilisation of heavy metals from the sludge, the bioleaching process resulted in the undesirable dissolution/loss of sludge bound nutrients (nitrogen and phosphorus), thus making the sludge less attractive for land application as a fertiliser. After 16 days of bioleaching about 45% of the nitrogen and 34% of the phosphorus were leached from the sludge using indigenous iron oxidising microorganisms, whereas about 78% of the nitrogen and 56% of the phosphorus were leached using indigenous sulphur oxidising microorganisms. The findings indicated that the fertilising property of the sewage sludge can be maintained by conducting the process for a shorter duration of time (up to 10 days). The optimum bioleaching period was 10 days where about 85%Cu, 71%Ni, 91%Zn and 61%Cr were solubilised from the sludge while the loss of nitrogen and phosphorus was only 56 and 51% respectively, by using sulphur oxidising microorganisms. The heavy metals remaining in the bioleached sludge were mostly in the residual fraction ensuring the safe disposal of bioleached sludge for land application as a fertiliser.
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( Ankur Kumar ),( Sudhir Kumar Dhama ),( Kuldeep Tyagi ),( Neeraj Kumar Tyagi ) 전북대학교 농업과학기술연구소 2011 농업생명과학연구 Vol.42 No.1
The present study was conducted to evaluate the genetic variability and correlation of different characters associated with grain yield in wheat. Forty genotypes were grown in randomized block design with three replications and evaluated for twelve yield and yield-related characters. Highly significant differences and adequate genetic variability were observed among the genotypes for all twelve characters. The heritability showed highest value for plant height, days to 50% flowering, days to maturity, peduncle length, 100 grain weight, number of spikelet per spike and number of grains per spikelet. Highest values of genetic advance were obtained in biological yield, plant height, days to maturity, peduncle length and days to 50% flowering. Correlation studies revealed an intensive selection for number of tillers, days to 50% flowering, days to maturity, number of spikelet per spike, number of grains per spikelet and 100 grain weight will contribute to improve the seed yield in wheat. Path analysis showed that maximum direct effect on grain yield per plant was contributed mostly by biological yield, followed by harvest index, spike length, plant height and days to 50% flowering. Principal components analysis (PCA) was performed and the first five principal components explained 75% of the observed variation. PC1 accounted for 23% of variation and showed the largest loading values for number of tiller`s per plant, biological yield and grain yield per plant. PC2 accounted for 17% and associated with plant height, peduncle length and harvest index. PC3 accounted for 13% and related to the days to 50% flowering and days to maturity. PCA analysis showed that wheat genotypes HPB42, UP2123, PBW527 and HD3024 showed good performance among wheat genotypes on the basis of all traits combined. The identified genotypes may be useful for wheat yield improvement.
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Upregulated Myc Expression in N-Methyl Nitrosourea (MNU)-induced Rat Mammary Tumours
Barathidasan, Rajamani,Pawaiya, Rajveer Singh,Rai, Ram Bahal,Dhama, Kuldeep Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.8
Background: The most common incident cancer and cause of cancer-related deaths in women is breast cancer. The Myc gene is upregulated in many cancer types including breast cancer, and it is considered as a potential anti-cancer drug target. The present study was conducted to evaluate the Myc (gene and protein) expression pattern in an experimental mammary tumour model in rats. Materials and Methods: Thirty six Sprague Dawley rats were divided into: Experimental group (26 animals), which received the chemical carcinogen N-methyl nitrosourea (MNU) and a control group (10 animals), which received vehicle only. c-Myc oncoprotein and its mRNA expression pattern were evaluated using immunohistochemistry (IHC) and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), respectively, in normal rat mammary tissue and mammary tumours. The rat glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as internal control for semi-quantitative RT-PCR. Results: Histopathological examination of mammary tissues and tumours from MNU treated animals revealed the presence of premalignant lesions, benign tumours, in situ carcinomas and invasive carcinomas. Immunohistochemical evaluation of tumour tissues showed upregulation and heterogeneous cellular localization of c-Myc oncoprotein. The expression levels of c-Myc oncoprotein were significantly elevated (75-91%) in all the tumours. Semi-quantitative RT-PCR revealed increased expression of c-Myc mRNA in mammary tumours compared to normal mammary tissues. Conclusions: Further large-scale investigation study is needed to adopt this experimental rat mammary tumour model as an in vivo model to study anti-cancer strategies directed against Myc or its downstream partners at the transcriptional or post-transcriptional level.
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Analysis of structure-function relationship in porcine rotavirus A enterotoxin gene
Sharad Saurabh,Shubhankar Sircar,Jobin J. Kattoor,Souvik Ghosh,Nobumichi Kobayashi,Krisztian Banyai,Obli R. VinodhKumar,Ujjwal K. De,Nihar R. Sahoo,Kuldeep Dhama,Yashpal S. Malik 대한수의학회 2018 Journal of Veterinary Science Vol.19 No.1
Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the nsp4 gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of nsp4 in the porcine population. Full-length nsp4 genes were amplified, cloned, sequenced, and then analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein, and prediction of post-translational modification. RV presence was observed in both diarrheic and non-diarrheic piglets. All nsp4 genes possessed the E1 genotype. Comparison of primary, secondary, and tertiary structure and the prediction of post-translational modifications of NSP4 from diarrheic and non-diarrheic piglets revealed no apparent differences. Sequence analysis indicated that nsp4 genes have a multi-phyletic evolutionary origin and exhibit species independent genetic diversity. The results emphasize the evolution of the E9 nsp4 genotype from the E1 genotype and suggest that the diarrhea-inducing capability of porcine RVs may not be exclusively linked to its enterotoxin gene.
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