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권순정,손덕영,최윤호 한국의료복지시설학회 2008 의료·복지 건축 Vol.14 No.4
Construction and operation of Germ Free Pigs' facilities are very expensive because pigs' rooms and other major rooms of the facility require germfree environments. Especially, running the HVAC system of aseptic facilities requires a lot of expenses. However proper location and efficient shape of outlets/inlets for the ventilation of the room can reduce the excessive running cost. In order to do that, this study proposes alternative location and shape of ventilation outlets/inlets to the existing design pattern in germfree pigs' room. The design condition of this study is the maintenance of adequate temperature(24'C), NHs concentration level(below 1.5 ppm), and air stream speed(be1ow .25m/sec) in the pigs' room for the summer and the winter together. As the Software Program, FLUENT(Ver. 6.2) has been used for the analysis of proposed ventilation patterns, In conclusion, wall inlets and ceiling inlet/outlet are advisable in summer, wall inlets and ceiling outlets is advisable in winter. As far as the shape is concerned, diffuser type for the ceiling outlet is desirable.
권호근,김영남,정기호,송근배,박덕영 대한구강보건학회 2003 大韓口腔保健學會誌 Vol.27 No.3
The purpose of this study is to estimate the National Health Insurance budget needed for the Korean elderly's denture service coverage. For more exact estimating the budget, denture need and demand were calculated by using 2000 National Oral Health Survey data. The results were summarized as follows ; 1. Over 65 years old, full denture and partial denture need per arch were each 8.5% and 16.8%. And over 70 years old, those were each 11.3% and 17.8%. 2. Over 65 years old, full denture and partial denture demand per arch were each 22.8% and 21.2%. And over 70 years old, those were each 27.%% and 21.1%. 3. Based on the denture need, the health insurance budget needed in the first operative year was about 955.4 billion won, over 65 years old and about 644.2 billion won, over 70 years old. 4. Based on the denture demand(wear), the health insurance budget needed in the first operative year was about 207.5 billion won, over 65 years old and was about 151.6 billion won, over 70 years old. If health insurance policy of elderly's denture service coverage is performed, actual denture wear rate is going to increase, and the budget needed is also increasing. Because current National Health Insurance budget status is poor, it is difficult to execute health insurance coverage for elderly's denture service. Therefore we suggest that health insurance coverage for preventive service is to be considered in order to reducing denture need in the long term view.
감마선과 Methyl Bromide 처리가 사과의 생리화학적 품질에 미치는 영향
강호진,정헌식,조덕조,변명우,최성진,최종욱,권중호 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2003 한국식품저장유통학회지 Vol.10 No.3
감마선(0, 0.5, 1, 2, 3 kGy) 조사와 methyl bromide(MeBr, 26 g/㎏, 4hr) 훈증 및 이들의 처리시기가 사과의 생리화학적 품질특성에 영향을 비교하여, 검역처리를 위한 ◁그림 삽입▷ (원문을 참조하세요) 감마선 조사의 응용성을 검토하였다. 감마선과 MeBr 처리에 따른 사과의 생리화학적 품질평가에서 2kGy 이상의 감마선 조사와 MeBr 훈증은 사과의 일부 생리화학적 품질특성의 변화를 촉진시키는 것으로 나타났다. 감마선과 MeBr의 처리시기별로는 수확 직후보다는 저온(0℃)에서 40일 정도 보관 후 처리한 대체적으로 품질손상이 적은 것으로 나타났다. 따라서 1kGy 이하의 감마선 조사와 지연조사는 사과 과실의 품질을 비교적 양호한 상태로 유지할 수 있어 검역처리 기술로 활용성이 있는 것으로 생각된다. 'Fuji' apples were treated by gamma irradiation (0∼3 kGy) or methyl bromide fumigation at commercial conditions (MeBr, 25 g/㎏, 4 hr) for quarantine purpose at two different treatment timing after harvest and stored under air a 0℃ for 4 months. Associated with the treatment timing of irradiation or fumigation, after 40 days of storage at 0℃ following harvest was more adequate than immediately after harvest in keeping qualities of stored apples. However, more than 2 kGy irradiation and MeBr fumigation were detrimental to physiological and chemical qualities of the fruits. These results show that less than 1 kGy irradiation at the delayed timing has a possibility to be applied as a quarantine procedure without significant changes in the quality of apples.
Deok Ho Kwon,Kyung Mun Kim,Taek Joon Kang,Se Jin Kim,Byeong-Ryeol Choi,Si Hyeock Lee 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10
An easy and rapid resistance detection protocol for the Western flower thrips Frankliniella occidentalis was established based on the residual contact vial bioassay (RCV), in which insecticide resistance levels can be estimated at 8 h-post treatment of diagnostic doses. The RDA strain was used as a reference susceptible strain, which has been reared under laboratory conditions over 10 years without exposure to any insecticides. Seven insecticides were tested for the determination of diagnostic dose. Among them, five insecticides (chlorfenapyr, acrinathrin, spinosad, emmamectin benzoate and thiamethoxam, ranged as 0.03 ~ 0.42 μg-1cm2) were applicable to the RCV. However, two insecticides (omethoate and imidacloprid) were not able to be used for the RCV because the treated inner surface of glass vials by these insecticides were too viscous, causing non-specific mortality. The RCV detection kit was employed for the estimation of resistance levels for the five insecticides in five local populations. Almost field-collected populations revealed high levels of resistance to the four insecticides (acrinathrin, spinosad, emmamectin benzoate and thiamethoxam) by showing less than 50% mortality. The baseline resistance detection by RCV method will facilitate the selection of proper insecticides for farmers to manage insecticide resistant-populations of F. occidentalis.
Residual contact bioassay for the rapid diagnosis of acaricide resistance in Tetranychus urticae
Deok Ho Kwon,Jeong Joon Ahn,Joon Ho Lee,Si Hyeock Lee 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10
To establish a rapid diagnosis method for the monitoring of acaricide resistance in the two-spotted spider mite, Tetranychus urticae, we evaluated the performance of residual contact vial (RCV) method as a routine bioassay for T. urticae by using two widely used acaricides, abamectin and tebupenpyrad. Appropriate concentrations of test acaricides were dissolved in acetone and evenly coated (100 μl) onto the inside wall of a 4-ml glass vial using a rolling machine. The average survival times in untreated control vial was longer than 12 hrs in the absence of food or water regardless of cap being closed or open. Webbing behavior of mites inside the vial, which may interfere with maximum chemical contact, began to be observed from 8 hrs post treatment. The minimum concentrations causing 100% mortality within 8 hrs posttreatment in a susceptible strain of T. urticae were determined to be 30 and 60 ppm in abamectin and tebupenpyrad, respectively. Dose-response curve was significantly affected by temperature in both acaricides, in which the knockdown rate increased greatly as temperature increased. The endpoint mortality at 6-8 hrs posttreatment, however, was not significantly affected by temperature. Nymphal stage of mites showed more rapid intoxication response than adults but endpoint mortality at 6-8 hrs posttreatment was not substantially different between developmental stages. When compared with the results from conventional spray method, RCV method showed moderate to high correlation coefficients (r=0.51~0.98), suggesting that it is a reliable in determining susceptibility of T. urticae. The vial-coated pesticides were stable at least one year when stored at -20°C as determined by LC-MS/MS analysis. Moreover, there was no significant difference in the bioassay results in repeated experiments with three different persons, indicative of high reproducibility of RCV. The RCV diagnostic kit, when used by farmers on site, should provide crucial and essential information for the selection of most suitable acaricides for different field populations of T. urticae.
Screening of lethal dsRNA and identification of dsRNA responsible genes in Tetranychus urticae
Deok Ho Kwon,Ji Hyun Park,Patil Anandrao Ashok,Unggyu Lee,Si Hyeock Lee 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04
Tetranychus urticae is extremely hard to control by conventional acaricides due to its rapid development of resistance to nearly all arrays of acaricide. As an alternative control measure of acaricide-resistant mites, RNA interference (RNAi)-based method has recently been suggested. A double-stranded RNA (dsRNA) delivery method using multi-unit chambers was established and employed to screen the RNAi toxicity of 42 T. urticae genes. Among them, the dsRNA treatment of coatomer I (COPI) genes, such as coatomer subunit epsilon (COPE) and beta 2 (COPB2), resulted in high mortality [median lethal time (LT50) = 89.7 and 120.3 h, respectively]. The transcript level of the COPE gene was significantly (F3,9 = 16.2, P = 0.001) reduced up to 24% following dsRNA treatment, suggesting that the toxicity was likely mediated by the RNAi of the target gene. To identify the deferentially expressed gene upon dsRNA ingestion, RNA-seq was employed to compare the transcriptional profiles between mites fed dsEGFP and dsCOPB2. Approximately 928 of genes were up- or down-regulated significantly (P < 0.05) compared to control and 182 genes were commonly responded to the treatment of both dsRNAs. Those dsRNA-responsible genes were mainly categorized into metabolic enzymes, transporters and secretory proteins. Further study would be necessary to elucidate the roles of dsRNA-responsible genes in mite’s dsRNA uptake and defense.