In vitro Meristem Tip Culture of Rosa hybrida

Debasis Chakrabarty,Subodh Kumar Datta 한국원예학회 2006 Horticulture, Environment, and Biotechnology Vol.47 No.2

A micropropagation method for Rosa hybrida cv. Contempo and Mrinalini is presented using shoot tip proliferation onto Murashige and Skoog (MS) medium with 0.2 ㎎ㆍL?¹ indole-3-acetic acid (IAA) along with different concentrations of 6-benzylaminopurine (BAP). Percent of explant producing multiple shoots, number of shoots per responded explant and growth of shoots varied with BAP concentration. The optimum results were recorded in 2 ㎎ㆍL?¹ BA. Addition of AgNO₃ in the medium reduced leaf abscission of the in vitro developed shoots. The regenerated plants were transferred to rooting medium (×1/4 MS with 0.1 ㎎ㆍL?¹ IAA, 0.1 ㎎ㆍL?¹ indole-3-butyric acid, 30 gㆍL?¹ sucrose, and 0.6% agar). The highest percentage of root formation was observed in ‘Contempo’ (57.1%), whereas very low percentage of rooting (16.6%) was observed in ‘Mrinalini’. When rooting medium was supplemented with different concentrations of activated charcoal, increase in rooting percentage with increasing the concentration was recorded in the case of ‘Mrinalini’. At the end of 21-28 days, however, the shoots were deteriorated due to yellowing of leaflets. When the plantlets were rooted in liquid medium, on the contrary, the percentage of yellowing leaflets was almost zero. The resulting plantlets were acclimatized and grown in potted soil where they developed normally.

Analysis of prismatic springs of non-circular coil shape and non-prismatic springs of circular coil shape by analytical and finite element methods

Arkadeep Narayan Chaudhury,Debasis Datta 한국CDE학회 2017 Journal of computational design and engineering Vol.4 No.3

This paper presents a methodology for designing prismatic springs of non-circular coil shape and nonprismatic springs of circular coil shape using analytical and numerical methods. To start with, simple analytical formulations for obtaining the axial deformation of the springs under axial load have been demonstrated. Next, the processes of obtaining CAD models of the springs and their subsequent finite element analysis (FEA) in commercial softwares have been outlined. In the third part, the different springs have been compared with a common cylindrical spring and their merits compared to a common spring have been demonstrated. Next, a fairly accurate analytical formulation (with maximum error of ?7–8%) for obtaining the value and location of maximum shear stress for all the springs has been demonstrated. Next, two aspects of non-prismatic springs under dynamic loads, viz. damping introduced in a vibrating system and contribution of the spring to the equivalent mass in a one dimensional vibrating spring mass system due to shape of the spring have been discussed. The last part involves an analytical formulation for the linear elastic buckling of two springs with circular coil shapes. For the majority of the work, emphasis has been on obtaining and using closed form analytical expressions for different quantities while numerical techniques such as FEA have been used for validation of the same.

Advanced Surface Engineering Approaches for Exotic Applications

Sutanuka Mohanty,Soumyabrata Basak,Debasis Saran,Kajari Chatterjee,Turin Datta,Atul Kumar,Chandra Prakash,Doo-Man Chun,Sung-Tae Hong,Kisor Kumar Sahu 한국정밀공학회 2024 International Journal of Precision Engineering and Vol.25 No.2

Selection and classification of suitable materials for exotic applications, including aviation/space, nuclear, oil &gas industries, are still challenging since these applications are typically very demanding in terms of microstructural, mechanical, and electrochemical properties. Surface engineering plays an important role in controlling the properties of the exposed surface, which play key roles in interfacing with another material or exposure to the environment, promoting desirable chemical reactions, and suppression of corrosion. Hence, modification of the surface characteristics of desired materials to the desired depth without altering their bulk properties is essential. The present review focuses on using some of the latest and most advanced surface engineering tool sets such as laser, electron beam (EB), friction stir processing (FSP), ion beam, and plasma immersion ion implantation (PIII). Such techniques provide substantial improvements in wear and corrosion resistance, reduction in frictional losses, increased fatigue life, and enhanced chemical stability at high temperatures for exotic applications.

Use of RAPD Analysis to Determine Genetic Diversity and Relationships among Bougainvillea Cultivars at Intra- and Inter-Specific Levels

Jolly Chatterjee,Abul Kalam Azad Mandal,Debasis Chakrabarty,Subodh Kumar Datta 한국원예학회 2007 Horticulture, Environment, and Biotechnology Vol.48 No.1

This study was aimed at determining the genetic relationships among the most important Bougainvillea cultivars grown at the National Botanical Research Institute, Lucknow, India using random amplified polymorphic DNA (RAPD) technology. Fifty random decamer primers were screened and ten were selected for final RAPD analysis. Each primer produced a unique set of amplification products ranging in size from 300 to 2500 bp. The number of bands for each primer varied from 10 in P2 to 18 in P10. These ten primers used in this analysis yielded 167 scorable bands with an average of 11.3 bands per primer. Of the 167 fragments scored from these primers, 26 were monomorphic and 141 were polymorphic (84.4%). The generated similarity matrix showed that the genetic diversity within the tested genotypes was high (average similarity index = 30.1%). Similarity values among the studied genotypes ranged between 6 to 89%. The resulting dendrogram divided the cultivars into two main clusters. The first contained 37 cultivars and was divided into two subclusters at a similarity value of 0.03 with 21 and 16 cultivars in each subcluster, respectively. Subcluster Ⅰ included those cultivars which had affinity with Buttiana group. Subcluster Ⅱ contained cultivars of both Spectabilis and Peruviana groups. The second major cluster contained 55 cultivars, contain species of Glabra group and cultivars whose origin are not well recorded. The similarity data obtained in this study agree to some extent (at least for the Buttiana group) with the previous classification of the tested genotypes. The information obtained from this work may be useful for better management, identification of accessions and also in avoiding duplications or mislabeling of the genotypes studied.

Prolificacy and Its Relationship with Age, Body Weight, Parity, Previous Litter Size and Body Linear Type Traits in Meat-type Goats

Haldar, Avijit,Pal, Prasenjit,Rajesh, M. Datta,Pal, Saumen K.,Majumdar, Debasis,Biswas, Chanchal K.,Pan, Subhransu Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.5

Data on age and body weight at breeding, parity, previous litter size, days open and some descriptive body linear traits from 389 meat-type, prolific Black Bengal goats in Tripura State of India, were collected for 3 and 1/2 years (2007 to 2010) and analyzed using logistic regression model. The objectives of the study were i) to evaluate the effect of age and body weight at breeding, parity, previous litter size and days open on litter size of does; and ii) to investigate if body linear type traits influenced litter size in meat-type, prolific goats. The incidence of 68.39% multiple births with a prolificacy rate of 175.07% was recorded. Higher age (>2.69 year), higher parity order (>2.31), more body weight at breeding (>20.5 kg) and larger previous litter size (>1.65) showed an increase likelihood of multiple litter size when compared to single litter size. There was a strong, positive relationship between litter size and various body linear type traits like neck length (>22.78 cm), body length (>54.86 cm), withers height (>48.85 cm), croup height (>50.67 cm), distance between tuber coxae bones (>11.38 cm) and distance between tuber ischii bones (>4.56 cm) for discriminating the goats bearing multiple fetuses from those bearing a single fetus.