Biodegradation of Di-n-Butyl Phthalate by Rhodococcus sp. JDC-11 and Molecular Detection of 3,4-Phthalate Dioxygenase Gene

( De Cai Jin ),( Ren Xing Liang ),( Qin Yun Dai ),( Rui Yong Zhang ),( Xue Liu Wu ),( Wei Liang Chao ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.10

Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 were 8.0, 30oC, and 175 rpm, respectively. In addition, low concentrations of glucose were found to inhibit the degradation of DBP, whereas high concentrations of glucose increased its degradation. Meanwhile, a substrate utilization test showed that JDC- 11 was also able to utilize other phthalates. The major metabolites of DBP degradation were identified as monobutyl phthalate and phthalic acid by gas chromatographymass spectrometry, allowing speculation on the tentative metabolic pathway of DBP degradation by Rhodococcus sp. JDC-11. Using a set of new degenerate primers, a partial sequence of the 3,4-phthalate dioxygenase gene was obtained from JDC-11. Moreover, a sequence analysis revealed that the phthalate dioxygenase gene of JDC-11 was highly homologous to the large subunit of the phthalate dioxygenase from Rhodococcus coprophilus strain G9.

不栺方鼎時代「穆王說」補

張德良(Zhang De Liang) 경북대학교 아시아연구소 2010 아시아연구 Vol.- No.9

‘不栺方鼎’이 만들어진 시기에 대해서는 현재 周昭王시기, 周穆王시기 그리고 周恭王.周懿王시기라는 세 가지 견해가 상존하고 있다. 본 논문은 考古類型學의 방법을 활용하여, 함께 출토되었던 器物.器形,紋樣및 書體등의 네 가지 각도에서 고찰했다. 함께 출토되었던 器物로는 서로 다른 형태를 띠고 있는 두 개의 銅戈가 있다. 하나는 穆王시기에 해당하는 伯墓에서 출토된 微胡一穿戈이고, 또 하나는 西周中期에 해당하는 天馬—曲村M7092에서 출토된 長胡三穿戈이다. 井中偉박사의 연구에 의하면, 전자의 유행은 그 年代의 下限線을 西周시대 중기의 초기단계로 잡을 수 있고, 후자의 유행은 西周시대 중기까지로 볼 수 있다. 그리고 陝西省長安縣張家坡의 西周시대 무덤 M284에서 출토된 咸作豐大母方鼎은 이 ‘不栺方鼎’과 그 器形이 매우 유사한데, 그 시기는 서주시대 중기에 해당한다. 또한 이 ‘不栺方鼎’과 유사한 紋樣을 가진器物로는 陝西省岐山縣董家村의 窖藏에서 출토된 二十七年衛簋를 들수 있는데, 그 시기는 穆王시기에 해당한다. ‘不栺方鼎’의 書體는 시기적으로 穆王시기에 해당하는 豐尊과 共王시기에 해당하는 墻盤의 중간지점에 놓여 있다고 볼 수 있다. 그 중에서 穆王시기에 해당하는 方鼎甲과 가장 근접하고 있는 편이다. 따라서 위에서 언급한 몇 가지 내용을 종합하여 필자는 이 ‘不栺方鼎’의 시기를 西周시대 중기의 초기단계인 穆王시기로 잡았다. 이러한 時代區分은 周原甲骨文에 보이는 ‘不栺’의 활동연대와도 서로 일치하기 때문에, 이 사실도 필자의 견해에 또 하나의 중요한 증거가 되어주었다. 그 외에도 이 ‘不栺方鼎’의 시기를 穆王시기로 정하는 일은 상단에 새겨진 竊曲紋을 연구하는 데 있어서도 매우 중요한 의미를 갖는다. By now there are three kinds of opinion about the dating of the rectangular cauldron of Buzhi: the period of King Zhao, or King Mu or the period of King Gong and King Yih in Western Chou history. The paper studies the co-existing utensils、vessel shape、decoration and the style of calligraphy with the means of typology. Among the co-existing utensils, there were two kinds of dagger axe. One is tiny Hu(flange) with one apertures, which also appeared in the tomb of Dong in Shan’xi Province. The other is long hu(flange) with three apertures, which occurred in one tomb of Tianma-qucun in Shanxi Province. The period of the former is King Mu of Western Chou, and the latter is Middle of Western Chou based on the study of one doctoral dissertation by Jing Zhongwei. Similar rectangular cauldron for Feng Damu(豐大母) was found at the cemetery of Zhangjiapo of Western Chou, which was in the middle of Western Chou. As for the decoration, gui food container for Wei from the hoard of Western Chou at Dongjiacun in Qishan County is similar to the rectangular cauldron, and the period of the gui food container is generally acknowledged as King Mu. The period of the style calligraphy of the rectangular cauldron was between King Mu and King Gong, compared with the Zun for Feng from the Hoard at Zhuangbai and the pan for the scribe of Qiang, and it is closer to the rectangular cauldron for Dong. Based one the analysis above, we can draw a conclusion that the period of the rectangular cauldron for Buzhi was King Mu, and this agreed with the conclusion that one person called Buzhi from the oracle inscriptions of the Western Chou at Zhouyuan was living Between the early Western Chou and the middle Western Chou. This is one circumstantial evidence. Once we accept the opinion, it is very important for us to study the pattern of Qiequ(竊曲紋) of the bronze of Western Chou.

Prognostic Value of C-Reactive Protein in Esophageal Cancer: a Meta-analysis

Zheng, Tian-Liang,Cao, Ke,Liang, Cui,Zhang, Kai,Guo, Hai-Zhou,Li, De-Ping,Zhao, Song Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

Background: The classical inflammatory biomarker, C-reactive protein (CRP), has been identified to be related to progression of esophageal cancer. Some research showed that elevated pretreatment serum CRP indicated a poor prognosis, but results have been inconsistent. Materials and Methods: We searched the Medline, Embase and the Cochrane Central Search Library for suitable studies and a meta-analysis of eleven (1,886 patients) was conducted to examine the relationship between elevated serum CRP level and overall survival (OS) in esophageal cancer cases. Moreover, correlation analyses were conducted to assess links between pretreatment serum CRP level and tumor node metastasis (TNM) stage as well as T, N, M grade, respectively. Results: The pooled analysis showed that elevated pretreatment serum CRP level was significantly associated with poorer overall survival (HR 2.09, 95%CI 1.52-2.87, p<0.01). Subgroup analyses were conducted by "country", "cut-off value", "treatment" and "number of patients", and no single factor could alter the result. Elevated pretreatment serum CRP was significantly correlated with more advanced TNM stage and T, N, M grade respectively. Conclusions: Elevated pretreatment serum CRP levels are associated with poorer prognosis in esophageal cancer patients, and could serve as a useful biomarker for outcome prediction.

Synthesis, Characterization, Luminescence and Biological Activity of Two Lanthanide Complexes Involving Mixed Ligands

De-Yun Ma,Hai-Fu Guo,Liang Qin,Jun Xu 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.9

Two new isostructural dinuclear complexes, Ln2(4-cpa)6(bpy)2 (Ln = Eu (1); Tb (2), 4-cpa = 4-chlorophenylacetate, bpy = 2,2'-bipyridine), have been hydrothermally synthesized and characterized by IR spectroscopy, elemental analysis, thermogravimetric analysis (TGA), powder X-ray diffraction and single-crystal X-ray diffraction. The lanthanide ions are bridged by two bidentate and two terdentate carboxylate groups to give centrosymmetric dimers with Ln···Ln separations of 3.967(2) and 3.956(3) Å, respectively. Each metal atom is nine-coordinate and exhibits a distorted tricapped trigonal prismatic geometry. Three-dimensional fluorescence spectra show that both 1 and 2 emit bright red and green luminescence at room temperature, with long lifetimes of up to 0.369 ms (at 614 nm) and 0.432 ms (at 543 nm), respectively. Moreover, poor luminescence efficiency has been noted for complex 2. The 4-Hcpa ligand and complexes 1-2 have been screened for their phytogrowthinhibitory activities against Brassica napus L. and Echinochloa crusgalli L., and the results are compared with the activity of quizalofop-P-ethyl.

Investigation of the Photoinduced Anisotropy of the Bacteriorhodopsin/Polymer Composite Film by Using Probing-Pumping Beams of Various Wavelengths

Liang-Yao Chen,Su-Xing Pan,Yun-Fei Wu,Lai Wei,Yu-Xiang Zheng,You-Yuan Zhao,De-Wang Ma 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.53 No.4

We have investigated the probing beam transmitance due to the photon-induced anisotropy effect in the bacteriorhodopsin/polymer composite film(bR/PC film) by using the probing-pumping beam method under different laser wavelengths. By using a theoretical simulation, we give the characteristics of the birefringence Δn and the dichroism ΔD for various wavelengths of the probing and the pumping beams.

Positive biodiversity-productivity relationship predominant in global forests

Liang, Jingjing,Crowther, Thomas W.,Picard, Nicolas,Wiser, Susan,Zhou, Mo,Alberti, Giorgio,Schulze, Ernst-Detlef,McGuire, A. David,Bozzato, Fabio,Pretzsch, Hans,de-Miguel, Sergio,Paquette, Alain,H&eac American Association for the Advancement of Scienc 2016 Science Vol.354 No.6309

<P>The biodiversity-productivity relationship (BPR) is foundational to our understanding of the global extinction crisis and its impacts on ecosystem functioning. Understanding BPR is critical for the accurate valuation and effective conservation of biodiversity. Using ground-sourced data from 777,126 permanent plots, spanning 44 countries and most terrestrial biomes, we reveal a globally consistent positive concave-down BPR, showing that continued biodiversity loss would result in an accelerating decline in forest productivity worldwide. The value of biodiversity in maintaining commercial forest productivity alone-US$166 billion to 490 billion per year according to our estimation-is more than twice what it would cost to implement effective global conservation. This highlights the need for a worldwide reassessment of biodiversity values, forest management strategies, and conservation priorities.</P>

High power impulse magnetron sputtering for obtaining cubic aluminum nitride on aluminum

De-Chiuan LIOU,Man-Jia PO,Min-Yi CHEN,Ying-Hung CHEN,Ju-Liang HE 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8

Introducer Curving Technique for the Prevention of Tilting of Transfemoral Günther Tulip Inferior Vena Cava Filter

Liang Xiao,De-sheng Huang,Jing Shen,Jia-jie Tong 대한영상의학회 2012 Korean Journal of Radiology Vol.13 No.4

Objective: To determine whether the introducer curving technique is useful in decreasing the degree of tilting of transfemoral Tulip filters. Materials and Methods: The study sample group consisted of 108 patients with deep vein thrombosis who were enrolled and planned to undergo thrombolysis, and who accepted transfemoral Tulip filter insertion procedure. The patients were randomly divided into Group C and Group T. The introducer curving technique was Adopted in Group T. The post-implantation filter tilting angle (ACF) was measured in an anteroposterior projection. The retrieval hook adhering to the vascular wall was measured via tangential cavogram during retrieval. Results: The overall average ACF was 5.8 ± 4.14 degrees. In Group C, the average ACF was 7.1 ± 4.52 degrees. In Group T, the average ACF was 4.4 ± 3.20 degrees. The groups displayed a statistically significant difference (t = 3.573, p = 0.001) in ACF. Additionally, the difference of ACF between the left and right approaches turned out to be statistically significant (7.1 ± 4.59 vs. 5.1 ± 3.82, t = 2.301, p = 0.023). The proportion of severe tilt (ACF ≥ 10º) in Group T was significantly lower than that in Group C (9.3% vs. 24.1%, χ2 = 4.267, p = 0.039). Between the groups, the difference in the rate of the retrieval hook adhering to the vascular wall was also statistically significant (2.9% vs. 24.2%, χ2 = 5.030, p = 0.025). Conclusion: The introducer curving technique appears to minimize the incidence and extent of transfemoral Tulip filter tilting. Objective: To determine whether the introducer curving technique is useful in decreasing the degree of tilting of transfemoral Tulip filters. Materials and Methods: The study sample group consisted of 108 patients with deep vein thrombosis who were enrolled and planned to undergo thrombolysis, and who accepted transfemoral Tulip filter insertion procedure. The patients were randomly divided into Group C and Group T. The introducer curving technique was Adopted in Group T. The post-implantation filter tilting angle (ACF) was measured in an anteroposterior projection. The retrieval hook adhering to the vascular wall was measured via tangential cavogram during retrieval. Results: The overall average ACF was 5.8 ± 4.14 degrees. In Group C, the average ACF was 7.1 ± 4.52 degrees. In Group T, the average ACF was 4.4 ± 3.20 degrees. The groups displayed a statistically significant difference (t = 3.573, p = 0.001) in ACF. Additionally, the difference of ACF between the left and right approaches turned out to be statistically significant (7.1 ± 4.59 vs. 5.1 ± 3.82, t = 2.301, p = 0.023). The proportion of severe tilt (ACF ≥ 10º) in Group T was significantly lower than that in Group C (9.3% vs. 24.1%, χ2 = 4.267, p = 0.039). Between the groups, the difference in the rate of the retrieval hook adhering to the vascular wall was also statistically significant (2.9% vs. 24.2%, χ2 = 5.030, p = 0.025). Conclusion: The introducer curving technique appears to minimize the incidence and extent of transfemoral Tulip filter tilting.

RTN4 3'-UTR Insertion/Deletion Polymorphism and Susceptibility to Non-Small Cell Lung Cancer in Chinese Han Population

Lu, De-Yi,Mao, Xu-Hua,Zhou, Ying-Hui,Yan, Xiao-Long,Wang, Wei-Ping,Zheng, Ya-Biao,Xiao, Juan-Juan,Zhang, Ping,Wang, Jian-Guo,Ashwani, Neetika,Ding, Wei-Liang,Jiang, Hua,Shang, Yan,Wang, Ming-Hua Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Nogo protein, encoded by gene reticulon-4 (RTN4), includes three major isoforms by different splicing, named Nogo-A Nogo-B and Nogo-C. Nogo proteins play an important role in the apoptosis of cells, especially in tumor cells. RTN4 single nucleotide polymorphisms (SNPs) can influence the efficiency of transcription and translation thus being related with an individual's predisposition to cancer. The CAA insertion/deletion polymorphism (rs34917480) within RTN4 3'-UTR has been reported to be associated with many cancer types. In order to investigate the relationship between this polymorphism and susceptibility to non-small cell lung cancer (NSCLC) in the Chinese population, we conducted the present case-control study including 411 NSCLC patients and 471 unrelated healthy controls. The genotype distributions were significantly different between cases and controls (p=0.014). We found that the del allele could significantly increase NSCLC risk (ins/ins vs ins/del: p=0.007, OR 1.46, 95%CI=1.11-1.93; dominant model: p=0.004, OR 1.47, 95%CI=1.13-1.92 and allele model: p=0.008, OR 1.35, 95%CI=1.08-1.67). This association was stronger in participants over 60 years old, males and smokers. We therefore conclude that the CAA insertion/deletion polymorphism (rs34917480) contributes to non-small cell lung cancer risk in Chinese population. Age, sex and environmental exposure are also related to carcinogenic effects of rs34917480.

Preparation and characterization of poly(dimethylsiloxane)-polytetrafluoroethylene (PDMS-PTFE) composite membrane for pervaporation of chloroform from aqueous solution

Zhen-Liang Xu,De Sun,Bing-Bing Li 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.11

Hydrophobic polydimethylsiloxane - polytetrafluoroethylene (PDMS-PTFE) flat-sheet membranes for pervaporation (PV) of chloroform from aqueous solution were successfully fabricated by solution casting method. The structures and the performance of the membranes was characterized by X-ray diffraction (XRD), scanning electron microscope combined with energy dispersive X-ray spectroscopy (SEM-EDXS), Fourier transform infrared spectroscopy (FT-IR), thermal gravimetric analysis (TGA) and the tests of contact angle and mechanical properties. The adding of PTFE particles (<4 μm) in the PDMS matrix enhanced the crystallinity, hydrophobicity, mechanical strength and thermal stability of the membranes. The examinations showed that the PTFE filled PDMS membranes exhibited striking advantages in flux and separation factor as compared with unfilled PDMS membranes. All the filled PDMS membranes with different PTFE content showed excellent PV properties for the separation of chloroform from water. When the content of the PTFE additive in PDMS composite membrane was 30 wt%, membrane performance was the best at feed temperature 50 oC and permeate-side vacuum 0.101MPa. For the 30% PTFE-PDMS membrane, with the increase of the feed temperature from 30 to 60 oC, the total, water and chloroform fluxes as well as the separation factor increased,the apparent activation energy (ΔEa) of total, chloroform and water were 21.08, 66.65 and 11.49 KJ/mol, respectively,with an increase of chloroform concentration in the feed from 50 to 950 ppm, total, water and chloroform fluxes increased but the separation factor decreased.