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- 저자 : De Jin, Cai (검색결과 305 건)
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( Su Jin Lee ),( Youngdeuk Lee ),( Gun Hoo Park ),( Navaneethaiyer Umasuthan ),( Soo Jin Heo ),( Mahanama De Zoysa ),( Won Kyo Jung ),( Dae Won Lee ),( Hanjun Kim ),( Do Hyung Kang ),( Chulhong Oh ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.6
L-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the Lasparaginase gene (L-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of L-ASPG86 (L-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The L-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant L-asparaginase (r-L-ASPG86) showed optimum conditions at 37-40oC, pH 9. Moreover, r-L-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-LASPG86 was 687.1 units/mg under optimum conditions (37°C, pH 9, and 5 mM MnSO4).
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徐大振 진주산업대학교 1977 論文集 Vol.15 No.-
Data used in this study were obtained from surueys of business operations of 300 Karean irop farms in the calendar year 1975, in order to determine and measure the faitors affecting farm income. Farms included in this study were from a probobility sample and were selected on the basis of farm size and Jinju Agri and Ferestry Junior Technical college. Based upon the analysis in this study the fallowing conclusions may be made : 1. The size of farm business itself is in an important factor in determining family labor income. And yet it, is not always nececsary to increase size of farm business for a high income mininwm size of farm business for on efficient operation appears to be 14∼16 tanbo. 2. Production rates had be greatest effect on family labor income. To be efficcint it is advisable to have 3.5 to 4.0 suk of rice yield per tanbo. For high rates of production, it is also advisable to adopt intensive farming practices by using more inputs per unit of land and have a high degree of land utilization through multi-cropping. 3. In general, no critical problem in the use of labor is indicated. It should be remembered, however, that labor is the cheapest substitute for capital items. It is desirable to have 25 to 30 hop of rice yield per hour of labor and 2,500 to 3,500 won of receipts per 10 hours of labor.
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Characterization of rDNAs and tandem repeats in the heterochromatin of Brassica rapa.
Lim, Ki-Byung,de Jong, Hans,Yang, Tae-Jin,Park, Jee-Young,Kwon, Soo-Jin,Kim, Jung Sun,Lim, Myung-Ho,Kim, Jin A,Jin, Mina,Jin, Yong-Moon,Kim, Seog Hyung,Lim, Yong Pyo,Bang, Jae-Wook,Kim, Ho-Il,Park, Be Korean Society for Molecular Biology 2005 Molecules and cells Vol.19 No.3
<P>We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and meiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situ hybridization (FISH) of rDNA and pericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.</P>
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Characterization of rDNAs and Tandem Repeats in the Heterochromatin of Brassica rapa
Beom-Seok Park,임기병,Hans de Jong,Tae-Jin Yang,Jee-Young Park,Soo-Jin Kwon,Jung Sun Kim,Myung-Ho Lim,Jin A Kim,Mina Jin,Yong-Moon Jin,Seog Hyung Kim,임용표,방재욱,Ho-Il Kim 한국분자세포생물학회 2005 Molecules and cells Vol.19 No.3
We describe the morphology and molecular organization of heterochromatin domains in the interphase nuclei, and mitotic and eiotic chromosomes, of Brassica rapa, using DAPI staining and fluorescence in situhybridization (FISH) of rDNA and ericentromere tandem repeats. We have developed a simple method to distinguish the centromeric regions of mitotic metaphase chromosomes by prolonged irradiation with UV light at the DAPI excitation wavelength. Application of this bleached DAPI band (BDB) karyotyping method to the 45S and 5S rDNAs and 176 bp centromere satellite repeats distinguished the 10 B. rapa chromosomes. We further characterized the centromeric repeat sequences in BAC end sequences. These fell into two classes, CentBr1 and CentBr2, occupying the centromeres of eight and two chromosomes, respectively. The centromere satellites encompassed about 30% of the total chromosomes, particularly in the core centromere blocks of all the chromosomes. Interestingly, centromere length was inversely correlated with chromosome length. The morphology and molecular organization of heterochromatin domains in interphase nuclei, and in mitotic and meiotic chromosomes, were further characterized by DAPI staining and FISH of rDNA and CentBr. The DAPI fluorescence of interphase nuclei revealed ten to twenty conspicuous chromocenters, each composed of the heterochromatin of up to four chromosomes and/or nucleolar organizing regions.
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De-Jin Su,김영길 한국무역학회 2022 Journal of Korea trade Vol.26 No.4
Purpose – Along with the growing awareness of environmental sustainability, international green entrepreneurship is expected to realize international sustainable development (ISD) by introducing ecological innovation results, using fewer resources consumption, and reducing negative environmental impacts. Building upon contingency theory and social network theory, this paper attempts to explore the role of home country-based networks (technology ties and business ties) as contingent factors that might impact the effectiveness of entrepreneurial orientation (EO) in promoting ISD among green ventures. Design/methodology – Original data were collected from 127 green ventures in China based on the random sampling technique. These green ventures mainly focus on the lower use of energy or materials, lower CO2 emissions, and higher ecological benefits located in the Fujian province, which highlights green economic growth and economic cooperation with international markets. Moderated hierarchical linear regression analysis was conducted to test our hypotheses concerning the main relationship between EO (innovativeness, proactiveness, and risk-taking) and ISD as well as the moderating effects of home country-based networks (technology ties and business ties) on the main relationship. Findings – Empirical results indicate that: first, both innovativeness and proactiveness positively affect ISD; second, both technology ties and business ties at home might significantly strengthen the positive relationship between innovativeness (or proactiveness) and ISD. However, this study did not find significant moderating effects of technology ties or business ties at home on the relationship between risk-taking and ISD. Originality/value – Since previous literature often gives more focus on the host country context of social networks, this study shifts to a home country-based network context. Therefore, our research might enrich the international green entrepreneurship literature by empirically investigating the contingent value of home country-based networks in the relationship between EO and ISD in the context of an emerging economy such as China.
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Chinese Policy to Stimulate University - Industry Linkages in Nanjing
De-Jin Su,Dong-Won Sohn,Sunwoo Sohn 과학기술정책연구원 2013 STI Policy Review Vol.4 No.2
Rapid changes in the economic, social and academic environments often provide opportunities to develop new and advanced technologies. In China, recent literature on the role of universities suggests that university - industry linkages (UILs) play a substantial role in the development of high-tech industries. Since 1979 when the country became more open and underwent economic reform, Chinese central authorities, local governments, and universities have continued to set up various science and technology (S&T) policies to stimulate UILs, contributing to China’s technological progress and economic growth. This study examines the role of S&T policies on UILs such as transfer of technology, joint research, and spin-off creation with a particular focus on Nanjing University (NJU) in Jiangsu Province. Nanjing has over 53 universities, ranking it behind Beijing and Shanghai in terms of S&T and higher education opportunities. By adopting “institutional methodology,” this study contends that UILs not only benefit universities and industry but also society in terms of job training, consulting activities, joint research, R&D results commercialization, patent licensing, new business creation, and other aspects. Finally, we suggest that the Chinese experience, though with some problems, might enhance our understanding of how to stimulate UILs through the arrangement of various S&T policies.
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Suppression of Clubroot Formation in Chinese Cabbage by the Chitin Compost and Broth
Jin Rong De,Han Tae-o,Kim Yong-oong,Kim Kil-ong The Korean Society for Applied Biological Chemistr 2006 Journal of Applied Biological Chemistry (J. Appl. Vol.49 No.4
Chitin compost and broth were used to suppress club root. Individual cabbage seedlings were transplanted into pots(3500 ml) containing a mixture of 3% chitin compost and 50 ml of chitin broth (T1) or the same quantity control compost and control compost broth(T2). The media in each pot was then infected with Plasmodiophora brassicae. Samples were taken at 6, 7 and 8 weeks after transplanting. The population of chitinase producing bacteria in T1 was consistently larger than that observed in T2. Chitinase activity in the T1 rhizosphere was two-fold greater than that of T2 at each time point observed. Shoot dry weight, leaf number and leaf area in T1 were enhanced 20%, 10% and 12% relative to those seen in T2, respectively. The disease index and root mortality at 8 weeks after transplanting were reduced by 50% and 25% in T1 compared to T2, respectively. Results presented in this study are strongly indicative that chitin compost and broth suppress clubroot in Chinese cabbage.
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