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호밀(Secale cereale L .)Esterase 동위효소의 유전적 연구
방재욱,최혜운 한국유전학회 1990 Genes & Genomics Vol.12 No.2
Esterase isozymes (EST) in endosperm, root and shoot of rye seedlings were determined using polyacrylamide gel electrophoresis and the effect of B-chromosomes (Bs) on EST zymogram phenotypes was studied. The number and staining intensity of EST bands vary depending on the tissues applied. EST-A is present in all the tissues of the rye seedlings, whereas EST-B is revealed as the coleoptile-specific esterase. EST-C exhibits a two-banded phenotype. EST-D showed different band patterns among tissues. In leaf EST patterns of rye with Bs and without Bs, EST-D showed difference in pattern wtith the presence of Bs. Individuals without Bs have two specific EST bands, whereas plants with 2Bs and 4Bs have three and four specific EST bands respectively. The results reported in this paper lend evidence for the genetic effect of Bs on EST patterns in rye.
호밀 (Secale cereale L.)B染色體의 Transmission에 관한 硏究
方在旭 木浦大學校基礎科學硏究所 1984 基礎科學硏究誌 Vol.2 No.-
The transmission of B chromosomes in PALDANG rve(Secale cereale L.) was investigated. In the progeny of the cross in population of Bs plants average number of B chromosome per plant increased 2 to 2.5 in first generation. In the progeny of 2Bs×2Bs, the B chromosome number per plant was found to be 2.1 and in the case of the crosses between 4Bs( ♀) and 2Bs (♂), it appeared 3.5, 3.1 and 3.2 respectively.
방재욱 한국유전학회 1988 Genes & Genomics Vol.10 No.2
The seedling proteins of rye were investigated by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis. Also the difference of electrophoretic pattern related to the presence of B chromosomes was studied. In SDS-PAGE gels, mass differences in high molecular weight proteins were observed between plants with B and without B chromosomes. More than 90 polypeptide spots were scored on 2-D gels. In comparison of 2-D electrophoretic patterns between seedlings with and without B chromosomes, mass modifications were found in 3 polypeptides on gels.
方在旭,吳炅煥 충남대학교 자연과학연구소 1980 學術硏究誌 Vol.7 No.1
Pinus densifiora의 體細胞 染色體數는 2n = 24이며 모든 染色體가 metacentric type이었다. Total length는 22.4∼13.6 μ이고 5雙의 染色體가 2次狹窄을 가지고 있었다. 2次狹窄을 가진 染色體는 같은 소나무屬內의 다른 種과 差異를 나타내었다. Pinus densiflora의 核型은 다음과 같이 表示된다. K=(2n=24)=2A^(m)+2B^(m)+2C^(m)+2D^(m) +2E^(m)+2F^(m)+2G^(m)+2(S)H^(m)+2_(S)I^(m) +2_(S)J^(m)+2_(S)K^(m)+2_(S)L^(m) The somatic chromosome number of Pinus densiflora was 2n=24. All chromosome pairs were metacentrie type with the range of total length from 22.4 to 13.6μ. Five pairs of chromosomes had the secondary constriction. The karyotype shows that the chromosome morphology of SAT-chromosome pairs in P. densiflora is different from other species in Pinus. The karyotype can be described as follow: K = (2n = 24) = 2A^(m)+2B^(m)+2C^(m)+2D^(m)+2E^(m)+2F^(m)+2G^(m)+2^(S)H^(m)+2_(s)I^(m)+2_(s)J^(m)+2_(s)K^(m)+2_(s)L^(m)