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새로운 Acetylcholinesterase inhibitors의 합성
최순규,김형민,조승환,최학기,박유미,이용균,정대일,김인식,한정태 동아대학교 부설 기초과학연구소 2004 基礎科學硏究論文集 Vol.21 No.1
노인성 치매의 일종인 alzheimer's disease의 효과적인 억제제를 합성하기 위해 우리는 분자 모델링에 의한 가장 적합한 물질인 m-[(N,N,N-trimethylammonio)phenyl]boronic acid를 합성하였다. 출발물질인 3-aminophenylboronic acid monohydrate의 경우 boronic acid의 작용기인 hydroxyl group을 protecting시킴으로써 반응의 안정성을 기하였다. Quarternary ammonium salt는 과량의 methyliodide와 염기 촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시켜 상당히 높은 수율을 얻을 수 있었다. 또한 과량의 methyliodide와 염기촉매인 potassium hydrogen carbonate를 사용하여 용매인 methanol에서 반응시킨 결과 boronic acid의 protection없이도 안정하게 반응이 진행되어짐을 확인할 수 있었다. In order to syntheisize a effective inhibotor for alzheimer's disease, we synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 which is designed by molecular modeling form. We protected the hydroxyl group of 3-aminophenylboronic acid monohydrate 1 with ethlyne glycol to remove the reactivity if hydroxyl group. To synthesize m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4, we reacted 3-aminophenylboronic acid monohydrate 1 with ezcess methyl iodide and potassium hydrogen carbonate as a base-catalyst in methanol. but On executed reaction without protection on hydroxyl group, we found out the result that the hydroxyl group of boronic acid group at 3-aminophenylboronic acid monohydrate 1 didn't react with excess methyliodide. Synthesized m-[(N,N,N-trimethylammonio)phenyl]boronic acid 4 is in progress about biological tests as a plausible acetylcholinesterase inhibitor.
배양한 사람 세포의 절제동복에 미치는 자외선과 MMS 의 이중효과
최경희,박상대,홍승환 한국유전학회 1980 Genes & Genomics Vol.2 No.1
DNA excision repair was measured in cultured normal human fibroblasts HF_1 after dual treatments with UV-light and MMS in order to determine the repair pathways for these agents. The results obtained were as follows: (1) The dose response of unscheduled DNA synthesis induced by 10-30J/㎡ UV-light or 0.5-5 mM MMS in normal human fibroblasts was dose dependent. However, the amount of unscheduled DNA synthesis in the combined treatment with MMS and UV-light was found to be much less than the sum of those treated separately, and even less than that produced by UV-light alone. This result indicates that MMS inhibits UV-induced unscheduled DNA synthesis. (2) Pyrimidine dimers produced by 26J/㎡ UV-light continued to be excised until 48hours, revealing 54% of the original dieters being remained unexcised. However, in 1 mM or 2 mM MMS treated groups prior to UV-irradiation, the unexcised dimer contents were found to be 69% and 80%, respectively. These results indicate that MMS inhibits the excision of pyrimidine dieters induced by UV-light.
Choi, Dae-Sung,Shin, Sung-Ho,Kim, Yun-Bae,Cha, Seung-Hee,Sok, Dai-Eun Korean Society for Biochemistry and Molecular Biol 1995 Journal of biochemistry and molecular biology Vol.28 No.1
Exposure of spleen lymphocytes to 2-chloroethylethyl sulfide (CEES) leads to a reduction of the intracellular ATP level, followed by a decrease in cell viability. Addition of nicotinamide, an inhibitor of poly(ADP-ribose) polymerase (PADPRP), restores both ATP level and viability, indicating that an activation of PADPRP is responsible for the cytotoxicity of CEES. The involvement of a $Ca^{2+}$-mediated process in cytotoxicity is suggested. Verapamil, EGTA, trifluoperazine, and butacaine exhibit a partial protection (20 to 58%) against the cytotoxicity of CEES. Investigation of the causative role of proteolytic degradation in cell death indicate that pepstatin and leupeptin exert a substantial protective effect (60 to 70%), suggesting the involvement of lysosomal destabilization in CEES-induced cytotoxicity. Also, lysosomotropic agents markedly decrease the cytotoxicity. Lysosomal labilization may be a mechanism for the cytotoxicity of CEES.