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Yoon, Dae-Wi,Gross, Dustin E.,Lynch, Vincent M.,Sessler, Jonathan L.,Hay, Benjamin P.,Lee, Chang-Hee WILEY-VCH Verlag 2008 Angewandte Chemie Vol.47 No.27
<B>Graphic Abstract</B> <P>Weak but important: The chloride anion binding properties of diametrically strapped calixpyrroles bearing benzene (see structure), pyrrole, and furan moieties in the strap have been studied in the solid state, in solution, and through theoretical analyses. The results obtained provide support for the notion that C&n.bond;H⋅⋅⋅Cl<SUP>−</SUP> hydrogen bonds are significant and contribute substantially to the Cl<SUP>−</SUP> binding energetics. <img src='wiley_img/14337851-2008-47-27-ANIE200801426-content.gif' alt='wiley_img/14337851-2008-47-27-ANIE200801426-content'> </P>
Yoon, Dae-Wi,Gross, Dustin E.,Lynch, Vincent M.,Lee, Chang-Hee,Bennett, Philip C.,Sessler, Jonathan L. Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.9
<P>The use of a pyrrole-strapped calix[4]pyrrole (1) permits the determination of chloride anion concentrations in mixed aqueous DMSO-<I>d</I><SUB>6</SUB>–H<SUB>2</SUB>O environments <I>via</I> proton NMR spectroscopy.</P> <P>Graphic Abstract</P><P>A pyrrole-strapped calixpyrrole allows for binding of chloride anion in a highly polar medium consisting of DMSO–H<SUB>2</SUB>O and applications in NMR spectroscopic-based determinations of [Cl<SUP>−</SUP>]. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b818077f'> </P>
Yoon, Dae-Wi,Gross, Dustin E.,Lynch, Vincent M.,Sessler, Jonathan L.,Hay, Benjamin P.,Lee, Chang-Hee WILEY-VCH Verlag 2008 Angewandte Chemie Vol.120 No.27
<B>Graphic Abstract</B> <P>Schwach und doch wichtig: Die Bindung des Chloridanions durch Calixpyrrole mit diametraler Brücke, die eine Benzol- (siehe Struktur; grau C,H; blau N; rot O; grün Cl), Pyrrol- oder Furaneinheit enthält, wurde im Festkörper, in Lösung und mithilfe theoretischer Analysen untersucht. Die Ergebnisse sprechen dafür, dass CH⋅⋅⋅Cl<SUP>−</SUP>-Wasserstoffbrücken wichtig sind und wesentlich zur Cl<SUP>−</SUP>-Bindungsenergetik beitragen. <img src='wiley_img/00448249-2008-120-27-ANGE200801426-content.gif' alt='wiley_img/00448249-2008-120-27-ANGE200801426-content'> </P>
Wi, Seung Gon,Lee, Kwang Ho,Park, Byung Dae,Park, Young Goo,Kim, Yoon Soo 한국목재공학회 2004 목재공학 Vol.32 No.3
The present work was undertaken to investigate the anatomical and chemical characteristics of transgenic poplar down-regulated with antisense OMT gene. Also the distribution of lignin in transgenic poplar trees was investigated at cellular level. No nisible abnormal phenotype was observed in the fibers and vessel elements of transgenic poplar. Any marked differences in the staining intensitie of Wiesner and Maule color reaction were not identified in the transgenic poplar. TEM micrographs did not show any staining intensities in the cell walls stained with KMnO_(4). Interestingly, the UV spectroscopy of semi-thin sections exhibited a distinct decrease of lignin absorption at 280 nm in the vessel walls, indicating transgenic poplar wood with lower amount of guaiacyl lignin in vessel elements. Chemical composition of antisense OMT poplar was almost identical to that of wild-type poplar. Klason lignin content of transgenic poplar did not show any significant difference from that of the controls. The solid state NMR spectra revealed the transgenic poplar with only slightly more syringyl lignin that the control. The present work showed that antisense OMT gene constructed in the poplar was not enough to reduce the overall content of Klason lignin, and suggested that the expression of transformation was confined to vessel walls.
Lee, Dae-Seok,Wi, Seung-Gon,Lee, Yoon-Gyo,Cho, Eun-Jin,Chung, Byung-Yeoup,Bae, Hyeun-Jong Humana Press 2011 Molecular biotechnology Vol.49 No.3
<P>A gene (arf) encoding an α-L: -arabinofuranosidase (ARF) that hydrolyzes arabinose substituted on xylan was isolated from Penicillium sp. The gene was predicted to encode 339 amino acid residues showing 71-75% homology to GH family 54. E. coli expressed ARF showed optimal activity at 50C and pH 5-6 on wheat arabinoxylan. The hydrolysis activities on oat spelt xylan by ARF and xylanase were 1.67-fold higher than that of xylanase alone. The synergistic effects of ARF and commercial enzymes (xylanase and cellulase) on popping-pretreated rice straw were 1.15-1.51-fold higher amounts of sugars released in the [ARF?+?xylanase?+?cellulase] mixture than in the mixtures [ARF?+?xylanase], [ARF?+?cellulase], and [xylanase?+?cellulase]. Moreover, the liberation of arabinose by ARF was enhanced 2.1-2.9-fold in a reaction with xylanase and cellulase as compared with [xylanase?+?cellulase] and ARF alone.</P>