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Evolutionary Strategy with an Aid of Biosensor for Violacein Production from Galactose
Da-ae GWON,Joo Yeon SEOK,Gyoo Yeol JUNG,Jeong Wook LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Violacein is a naturally occurring purple pigment with antibiotic properties. Violacein is synthesized from tryptophan, so sufficient tryptophan biosynthesis is the key to violacein production. However, tryptophan overproduction in Escherichia coli is challenging due to the complicated biosynthetic pathways and regulatory mechanisms. In this study, we used the adaptive laboratory evolution (ALE) strategy to improve violacein production from galactose. During the ALE, a tryptophan-responsive biosensor was employed to enrich tryptophan-producing cells. After six-rounds of evolution, we transformed plasmids for violacein production into evolved cells and screened violacein-overproducing cells with naked eyes. Consequently, we obtained the best violacein producer, which showed 2.7-fold higher titer compared to the parent strain. Whole-genome sequencing of the evolved strain identified several mutations. Among them, a missense mutation occurred on the coding sequence of galR, which may increase galactose utilization, leading to the increased production of violacein. Overall, we showed that the biosensor-assisted ALE strategy could be useful to rapidly and selectively evolve strains.
냉온 자극의 다양한 온도경계조건들에 대한 피부 내 온도 분포의 수치해석
박다애(Da Ae Park),오한나(Han Nah Oh),전병진(Byoung Jin Jeon),김은정(Eun Jeong Kim),이승덕(Seung Deok Lee),최형권(Hyoung Gwon Choi) 대한기계학회 2015 大韓機械學會論文集B Vol.39 No.11
본 연구에서는 냉온 자극요법의 열전달 특성을 정량적으로 파악하고자 한다. 축대칭 비압축성 Navier-Stokes 방정식 및 에너지 방정식의 해법을 위해 SIMPLE 알고리즘에 기반을 둔 유한체적법을 사용하였다. 혈액의 관류를 고려하기 위해 Pennes bio-heat 방정식을 추가로 적용하였다. 다양한 온도조건에 따른 피부 아래 각 지점에서의 열 침투양상을 분석하였다. 수치해석을 통하여 다양한 입력온도들에 대하여 역치 온도에 도달하는 영역들을 발견하였다. 또한, 페니스 효과로 인하여 피부 심부로 갈수록 외부 자극에 대한 온도변화가 완만함을 확인하였으며, 진피부분의 온도가 역치에 도달하기 위해서는 온자극 및 냉 자극은 47도 및 7도 내외로 주어져야 함을 발견하였다. 본 논문의 수치해석을 통해 구축된 데이터베이스는 경혈 냉온 자극 치료기의 최적설계 시에 활용된다. In this paper, the contrast therapy of skin was numerically investigated by solving the conjugate heat transfer problem. A finite volume method based on the SIMPLE algorithm was adopted to solve the axisymmetric incompressible Navier-Stokes equations, coupled with an energy equation. These equations are strongly coupled with the Pennes bio-heat equation in order to consider the effect of blood perfusion rate. We investigated the thermal response of skin at some selected depths for various input temperature profiles of a stimulator for contrast therapy. From the numerical simulations, the regions with cold/hot threshold temperatures were found for five input temperature profiles. It was shown that the temperature varies mildly for different input profiles as the depth increases, owing to the Pennes effect. The input temperatures for effective hot/cold stimulation of dermis layer were found to be 47℃ and 7℃, respectively. The present numerical results will be used for finding an optimal temperature profile of a stimulator for contrast therapy.
온냉요법 임상으로부터 얻은 다양한 온도경계조건들에 대한 피부 내 수치해석
오한나(Han Nah OH),박다애(Da Ae Park),전병진(Byoung Jin Jeon),김은정(Eun Jeong Kim),이승덕(Seung Deok Lee),최형권(Hyoung Gwon Choi) 대한기계학회 2015 대한기계학회 춘추학술대회 Vol.2015 No.11
In this paper, numerical simulations for the contrast therapy of skin were conducted by solving the conjugate heat transfer problem. To solve the axisymmetric incompressible Navier-Stokes equations and an energy equation, A finite volume method based on the SIMPLE algorithm was adopted in order to solve the axisymmetric incompressible Navier-Stokes equations coupled with an energy equation of skin. Pennes equation was also adopted in order to consider the effect of blood perfusion rate. We investigated temperature evolutions at selected depths of skin for various temperature profiles used in the clinical test conducted in our group. In addition, by comparing simulation results with clinical test data, we tried to find a relation between temperature evolution inside skin and the effect of the contrast therapy.
냉온자극의 다양한 온도경계조건들에 대한 피부 내 온도 분포의 수치해석
오한나(Han Nah Oh),박다애(Da Ae Park),김은정(Eun Jeong Kim),이승덕(Seung deok Lee),최형권(Hyoung Gwon Choi) 대한기계학회 2014 대한기계학회 춘추학술대회 Vol.2014 No.11
Contrast therapy of skin was numerically investigated by solving the conjugate heat transfer problem of the skin under the stimulator that periodically imposes hot and cold stimulations on the skin. A finite volume method based on the SIMPLE algorithm was adopted to solve the axisymmetric incompressible Navier-Stokes equations coupled with an energy equation. Those equations are strongly coupled with the Pennes bio-heat equation in order to consider the effect of blood perfusion rate. We investigated the thermal response of skin at some selected depths for various input temperature profiles of the stimulator. The present numerical results will be used in designing the optimal temperature profile of the stimulator.