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Cui Xi-Hua,Hosakatte Niranjana Murthy,Zhang Ji-De,Song Hang-Lin,Jiang Yin-Ji,Qi Wen-Wen,Li Yong Yi,백기엽,박소영 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.3
In this study, we aimed to verify the effect of nutritional factors on the accretion of secondary metabolites in the adventitious root (AR) cultures of Malaysian ginseng (Eurycoma longifolia Jack) grown in small-scale bioreactors. AR were induced from leaf explants and cultured in different types of media including Murashige and Skoog (MS) medium, Driver Kuniyuki Walnut (DKW) medium, Gamborg’s B5 medium, Woody Plant Medium (WPM), and ¾ MS medium. Among these media, the MS and Gamborg’s B5 media induced lateral root development from initial inoculum, which accounted for the increase in AR biomass accretion. By contrast, the DKW and WPM media did not induce lateral root formation from the cultured explants. The ¾ MS medium was optimal for the growth of AR and accretion of secondary metabolites, after 7 weeks of culture, the biomass of AR increased by 8.6-fold in ¾ MS medium, and the total phenolic and flavonoid contents reached 5.23 and 2 mg g−1 of tissue dry weight, respectively. Analysis of mineral elements in the spent medium revealed that ¾ MS medium was most suitable for nutrient supply to developing AR. LC–MS analysis showed the accretion of eurycomanone, a therapeutically useful metabolite, in the AR of Malaysian ginseng.
논문(論文) : 중국 농촌주택기지사용권의 자유처분에 관한 연구
( Cui Ji Zi ) 아세아여성법학연구소 2010 아세아여성법학 Vol.13 No.-
The usufruct of housing sites of China, which is a unique usufruct resulting from a land policy of ensuring the living of inhabitants in rural areas, means a kind of right to construct housing and ancillary facilities in housing sites and to retain the usufruct of them. Since the usufruct of housing sites has a dual character of property right and membership right and its living ensuring aim as well, the free disposal of this right has been limiting under existing laws. In recent years, however, the existing system of usufruct has failed to satisfy the rapid economic and social development because of the economic reform and the relaxation of household registration system, particularly the flow of labor force between urban and rural areas. It is to be regretted that there are not definite regulations laid down for the transferability of rural land under newly promulgated Property Law of the People`s Republic of China. Whether the usufruct of housing sites can be disposed freely has become a hot topic. Despite the courts held a negative attitude towards free disposal of the right, the problem is not entirely settled. This paper, therefore, aims to provide a possible solution to this hot controversy. First of all, an introduction to the development process and characteristics of the usufruct is present. Then, the second part will follow, which concerns the subjects of the right and the exercise methods. As the most important part of this paper, the third part deals with the controversy over whether the usufruct of housing sites can be disposed freely. Finally, the rea- sonable proposals for improving the usufruct system are put forward.
( Ji Hao Cui ),( So Ra Park ),( Byung Hyune Choi ),( Byoung Hyun Min ) 한국조직공학·재생의학회 2010 조직공학과 재생의학 Vol.7 No.1
This study investigated the effect of low intensity ultrasound (LIUS) stimulation of mesenchymal stem cells (MSCs) in vitro on the repair of cartilage defect after implantation of the construct in vivo. Rabbit MSCs were cultured in the polyglycolic acid (PGA) scaffold and preconditioned with (MSCs/US+) or without (MSCs/US-) LIUS stimulation during the chondrogenic differentiation for 1 week in vitro. The LIUS stimulation was carried out at the intensity of 200 mW/Cm2 every day for 20 min over a week. The constructs were implanted into the cartilage defects created in the rabbit femoral trochlea. The defect only was used as a negative control and rabbit chondrocytes seeded in PGA was used as a positive control, respectively. The repair of cartilage defect was examined at 2, 4, 8 and 12 weeks after implantation, respectively. The gross observation showed that the articular cartilage defects were filled with the repaired tissue in all groups. Histological and immunohistochemical analyses revealed, however, more intensive and widespread expression of proteoglycans and type II collagen in the MSCs/US+ group than in the MSCs/US- group. Fibrous tissues were observed mainly in the defect only group. The chondrocytes groups showed efficient repair of the defect by hyaline cartilage. In conclusion, this study suggested that LIUS preconditioning of MSCs in vitro could be an effective method to promote chondrogenesis of MSCs and repair of cartilage defect in vivo.
( Ji Hao Cui ),( Ho Geun Chang ),( Yong Chan Kim ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.2
This study investigated the changes in the growth and phenotype of human degenerated intervertebral disc cells depending on the frequency of subculture in vitro monolayer culture system. The annulus fibrosus (AF) and nucleus pulposus (NP) cells were cultured in a monolayer manner. At each subculture , we performed an analysis of the morphological changes, the adhesion rate, the proliferation rate and the viability. The expressions of type I, II collagen and proteoglycan were analyzed at the mRNA gene level. All three times of subculture were performed. Both the AF and NP cells gradually showed a fibroblast-like spindle shape from their irregular, polygonal shape while undergoing the third time of subculture. The cell proliferation was the highest at the second subculture time. The viability was markedly lower before the first subculture, but there was no significant difference of viability between the three times of subculture. On RT-PCR, the type II collagen expression was gradually decreased and almost unexpressed at the third subculture, but the type I collagen expression was gradually increased in the NP cells. In the AF cells, the type I collagen expression did not show a significant difference depending on the frequency of subculture. Type II collagen was not expressed from the second time of subculture. The expression of proteoglycan was gradually decreased in both the AF and NP cells and it was almost unexpressed at the third time of subculture. In conclusions, after three times of subculture, the disc cells had completely changed their original growth and phenotypic characteristics.
Government Role in the Internationalization of Accounting Standards in China
Cui Shun-Ji(최순희) 한국국제회계학회 2008 국제회계연구 Vol.0 No.24
계획경제체제에서 시장경제체제로 탈바꿈하여 겨우 30년을 경과한 중국에서 회계기준의 국제화는 장기간 자본주에 체제를 발전시켜 온 나라들에 비하여 한낮 어려운 과제로서 정부의 강력한 대응과 지원이 필요하다. 본 연구는 중국에서 회계기준의 국제화를 위한 정부의 지난 역할을 살펴보고 당면한 난제를 짚어 보면서 앞으로 정부의 회계기준의 국제화 전략을 제시하고자 한다. 중국이 회계기준의 국제화 과정에 부딪친 문제들은 정부와 회계학계 그리고 기타 사회단체들이 함께 노력해야 하지만 그 중에서 정부의 노력이 더욱 필요하다. 구체적으로 보면 정부의 노력이 아래와 같은 면에서 특별히 요청된다. 우선 큰 안목에서 회계기준의 국제화를 위한 정부의 중장기 목표와 계획이 수립되어야 하며 다음으로는 중국의 특정에 따라 법률, 시스템, 세무, 회계 등 다양한 영역의 협력을 지속시켜야 하며 그 다음으로는 더욱 긴밀한 국제협력을 통하여 중국 회계기준이 국제적인 인정을 받기 위한 노력을 기울려야 한다. 마지막으로 공인회계사 제도와 내부통제제도 등 제도건설을 강화함으로써 회계기준의 순조로운 국제화를 보장하여야 한다. 그 외에도 회계기준의 국제화를 위하여1000만명에 이르는 회계담당자에 대한 재교육도 무시할 수 없는 정부의 중요한 과제이다. It is only 30 years since China transformed its economic system from planned economy to market economy. So it is more difficult for China to realize the internationalization of accounting standards than the developed capitalist countries. China has to challenge and overcome its own special obstacles, and needs strong support and overall planning from its government. This paper mainly explains strategic measures used by Chinese government to internationalize the accounting standards, analyzes major problems we are currently facing and explores the role Chinese government should play in this important process. Though problems discussed above need to be solved by different sectors working together, what government do is of the most importance: Firstly, the government must set mid and long term goals and plans as the process of international compliance of accounting standards needs long term international cooperation; Secondly, taking the national conditions into account. further improve and coordinate laws. regulations, taxation, accounting, etc.: Thirdly, strengthen the international cooperation in a more extensive extent so as to realize the equal effects of China's accounting standards in the world; Lastly, keep on the regulation making of CPA and conclude the internal accounting control systems as the international compliance of accounting standards needs CPA systems. Besides, the internationalization of accounting standards makes it an indispensible part to further educate the present ten million or so accounting personnel.