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Claudia Concha,María Daniella Carretta,Pablo Alarcón,Ivan Conejeros,Diego Gallardo,Alejandra Isabel Hidalgo,Nestor Tadich,Dante Daniel Cáceres,María Angélica Hidalgo,Rafael Agustín Burgos 대한수의학회 2014 Journal of Veterinary Science Vol.15 No.2
Reactive oxygen species (ROS) production is one of the mainmechanisms used to kill microbes during innate immuneresponse. D-lactic acid, which is augmented during acuteruminal acidosis, reduces platelet activating factor(PAF)-induced ROS production and L-selectin shedding inbovine neutrophils in vitro. This study was conducted toinvestigate whether acute ruminal acidosis induced by acuteoligofructose overload in heifers interferes with ROSproduction and L-selectin shedding in blood neutrophils. Blood neutrophils and plasma were obtained by jugularvenipuncture, while ruminal samples were collected usingrumenocentesis. Lactic acid from plasma and ruminal sampleswas measured by HPLC. PAF-induced ROS production andL-selectin shedding were measured in vitro in bovineneutrophils by a luminol chemiluminescence assay and flowcytometry, respectively. A significant increase in ruminal andplasma lactic acid was recorded in these animals. Specifically,a decrease in PAF-induced ROS production was observed 8 hafter oligofructose overload, and this was sustained until 48 hpost oligofructose overload. A reduction in PAF-inducedL-selectin shedding was observed at 16 h and 32 h postoligofructose overload. Overall, the results indicated thatneutrophil PAF responses were altered in heifers with ruminalacidosis, suggesting a potential dysfunction of the innateimmune response.
Andres Torres,Reinhilde Jacobs,Paul Lambrechts,Claudia Brizuela,Carolina Cabrera,Guillermo Concha,María Eugenia Pedemonte 대한영상치의학회 2015 Imaging Science in Dentistry Vol.45 No.2
Purpose: This study used cone-beam computed tomography (CBCT) to characterize mandibular molar root and canal morphology and its variability in Belgian and Chilean population samples. Materials and Methods: We analyzed the CBCT images of 515 mandibular molars (257 from Belgium and 258 from Chile). Molars meeting the inclusion criteria were analyzed to determine (1) the number of roots; (2) the root canal configuration; (3) the presence of a curved canal in the cross-sectional image of the distal root in the mandibular first molar and (4) the presence of a C-shaped canal in the second mandibular molar. A descriptive analysis was performed. The association between national origin and the presence of a curved or C-shaped canal was evaluated using the chi-squared test. Results: The most common configurations in the mesial root of both molars were type V and type III. In the distal root, type I canal configuration was the most common. Curvature in the cross-sectional image was found in 25% of the distal canals of the mandibular first molars in the Belgian population, compared to 11% in the Chilean population. The prevalence of C-shaped canals was 10% or less in both populations. Conclusion: In cases of unclear or complex root and canal morphology in the mandibular molars, CBCT imaging might assist endodontic specialists in making an accurate diagnosis and in treatment planning.
Torres, Andres,Jacobs, Reinhilde,Lambrechts, Paul,Brizuela, Claudia,Cabrera, Carolina,Concha, Guillermo,Pedemonte, Maria Eugenia Korean Academy of Oral and Maxillofacial Radiology 2015 Imaging Science in Dentistry Vol.45 No.2
Purpose: This study used cone-beam computed tomography (CBCT) to characterize mandibular molar root and canal morphology and its variability in Belgian and Chilean population samples. Materials and Methods: We analyzed the CBCT images of 515 mandibular molars (257 from Belgium and 258 from Chile). Molars meeting the inclusion criteria were analyzed to determine (1) the number of roots; (2) the root canal configuration; (3) the presence of a curved canal in the cross-sectional image of the distal root in the mandibular first molar and (4) the presence of a C-shaped canal in the second mandibular molar. A descriptive analysis was performed. The association between national origin and the presence of a curved or C-shaped canal was evaluated using the chi-squared test. Results: The most common configurations in the mesial root of both molars were type V and type III. In the distal root, type I canal configuration was the most common. Curvature in the cross-sectional image was found in 25% of the distal canals of the mandibular first molars in the Belgian population, compared to 11% in the Chilean population. The prevalence of C-shaped canals was 10% or less in both populations. Conclusion: In cases of unclear or complex root and canal morphology in the mandibular molars, CBCT imaging might assist endodontic specialists in making an accurate diagnosis and in treatment planning.