FK506이 T 림프구 사멸에서 활성산소 생성에 미치는 영향

이호균(Ho Kyun Lee),정상영(Sang Young Chung),최수진나(Soo Jin Na Choi) 대한외과학회 2009 Annals of Surgical Treatment and Research(ASRT) Vol.77 No.5

Purpose: Tacrolimus (FK506) has been widely used as an immunosuppressant in organ transplanted recipients to suppress organ rejection phenomenon. We investigated the role of oxidative stress and heme oxygense-1 by FK506 on human Jurkat T cells. Methods: The cells viability was examined by DAPI stain, enzyme activity of caspase family proteins, and western blotting for Baks, PUMA, iNOS, HO-1. Cells were cultured in the absence or presence of CoPPIX or ZnPPIX and the fluorescence intensity was analyzed using a flow cytometry. Results: Treatment with FK506 increased the generation of reactive oxygen species (ROS), including hydrogen peroxide and superoxide anion, and NO in Jurkat cells in a dose-dependent manner. Immunohistochemistry and Western blot analysis data revealed the hemoxygenase-1 (HO-1) was induced by the addition of FK506 in Jurkat cells. Induction of CoPP, HO-1 inducer, resulted in decreased intracellular H₂O₂ and NO concentrations. Instead ZnPP, an HO-1 competitive inhibitor did it reversely. In addition, ZnPP regulates iNOS protein synthesis by inhibition of HO-1. Conclusion: Increase of HO-1 expression would induce to decrease the intracellular H₂O₂ and NO concentrations. Also, HO-1 would regulate iNOS protein synthesis. Consequently, we can expect the regulation of HO-1 expression with concomitants use of FK506 to suppress organ rejection phenomenon by enhancing apoptosis.

Impact of the GHG Reduction on Korean Economy Using Dynamic CGE

( Chung?sil Kim ),( Sang?ho Lee ) 한국농업경제학회 2006 KOREAN JOURNAL OF AGRICULTURAL ECONOMICS(KJAT) Vol.47 No.3

This study addresses on interactions of economy and environment in a dynamic computable general equilibrium (CGE) with a focus on greenhouse gas (GHG) mitigation strategy in Korea. Whether Korea has to reduce the greenhouse gas or not, it has large influence on Korea economy, such as changes in GDP and welfare. The GDP decreases by 0.113~0.132%, and the marginal abatement costs are 110~123 $US per metric ton of CO2 for Korea. In the case of reduction of greenhouse gas in Korea, the output in fossil fuel and energy.intensive sectors are decreased sharply. Therefore, we should be exempted or delayed from the international reduction programme of greenhouse gas abatement targets within the limits of the possible.

Fatigue Crack Growth Behavior in Ultrafine Grained Low Carbon Steel

Ho,Kyung Kim,Myung-Il Choi,Chin-Sung Chung Dong-Hyuk Shin 대한기계학회 2002 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.16 No.10

Ultrafine grained (UFG) low carbon (0.15 wt.% C) steel produced by equal channel angular pressing (ECAP) was tested for investigating the effect of load ratio on the fatigue crack growth rate. Fatigue crack growth resistance and threshold of UFG steel were lower than that of as-received coarse grained steel. It was attributed to the less tortuous crack path. The UFG steel exhibited slightly higher crack growth rates and a lower △K_th with an increase of R ratio. The R ratio effect on crack growth rates and △K_th was basically indistinguishable at lower load ratio (R>0.3), compared to other alloys, which indicates that contribution of the crack closure vanishes. The crack growth rate curve for UFG steel exhibited a longer linear extension to the lower growth rate regime than that for the coarse grained as-received steel.<br/>

형질전환을 위한 청치마의 재분화 체계확립

박태성(Tae Sung Park),조동욱(Dong Wook Cho),진명호(Myung Ho Jean),정규환(Kyu Hwan Chung) 한국원예학회 2000 원예과학기술지 Vol.18 No.5

신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

Curcumin protects retinal pigment epithelial cells against oxidative stress via induction of heme oxygenase-1 expression and reduction of reactive oxygen

Woo, Je Moon,Shin, Da-Yong,Lee, Sung Ju,Joe, Yeonsoo,Zheng, Min,Yim, Jin Ho,Callaway, Zak,Chung, Hun Taeg Molecular Vision 2012 Molecular vision Vol.18 No.-

<P><B>Purpose</B></P><P>To determine whether curcumin induces expression of the defensive enzyme heme oxygenase-1 (HO-1) and protects cells against oxidative stress in cultured human retinal pigment epithelial cells.</P><P><B>Methods</B></P><P>Effective concentrations and toxicities of curcumin were determined after 3 h of curcumin treatment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Confluent human retinal pigment epithelium cell lines (ARPE-19) were preincubated with curcumin and oxidatively challenged with H<SUB>2</SUB>O<SUB>2</SUB>. HO-1 expression was determined with western blot analysis. To confirm the protective role of HO-1 in oxidative stress, small interfering RNA (siRNA) against HO-1 or inhibitor of HO-1 was treated with curcumin in retinal pigment epithelium cells. Intracellular levels of reactive oxygen species (ROS) were measured with flow cytometry and confocal microscopy. Apoptosis was evaluated with Annexin V-fluoroscein isothiocyanate staining.</P><P><B>Results</B></P><P>Curcumin had little cytotoxicity at concentrations less than 30 μM, and HO-1 expression was the highest at the 15 μM concentration. At this concentration, curcumin also increased the cytoprotective effect against the oxidative stress of H<SUB>2</SUB>O<SUB>2</SUB> through the reduction of ROS levels in human retinal pigment epithelial cells. Curcumin’s effect on the reduction of ROS was mediated by the increase in HO-1 expression.</P><P><B>Conclusions</B></P><P>Curcumin upregulated the oxidative stress defense enzyme HO-1 and may protect human retinal pigment epithelial cells against oxidative stress by reducing ROS levels.</P>

부산·경남지역 사찰에서 수집된 차나무(Camellia sinensis L.)잎 형태별 분류

제순자(Je, Soon-Ja)･,이용호(Lee, Yong-Ho)･,오주성(Oh, Ju-Sung3)･,정영수(Chung, Yong-Soo)･,정대수(Chung, Dae-Soo) 한국차학회 2007 한국차학회지 Vol.13 No.1

본 시험은 부산·경남지역 사찰 주변에 자생하고 있는(또는 30년 이상 재배되어 온) 차나무를 중심으로 14개 지역을 선정하여 산지별로 차나무 잎을 채취, 수집하여 잎 형태별 특성을 조사하였다. 차나무 잎의 형태적 특성 중 엽장(葉長) (12.04㎝), 엽폭(葉幅)(4.90㎝), 엽면적(葉面積)(41.33㎝)은 사천 배방사지 차나무 잎이 가장 크게 나타났으며, 가장 작게 나타난 곳은 부산 전등사 차나무가 엽장(6.30㎝)과 엽폭(2.48㎝)이 작으며, 엽면적은 거제 봉곡사(14.80㎝) 차나무가 가장 작게 나타났다. 엽병장(葉柄長)은 밀양의 엄광사지(0.57㎝)와 사천 배방사지(0.57㎝) 차나무 잎이 가장 길게 나타났으며, 거치수(鋸齒數)는 사천 배방사지(30.13개) 차나무였으며, 엽맥수(葉脈數)는 하동 쌍계사(11.27개) 차나무로 가장 많게 나타났다. 생엽중(生葉重)과 건엽중(乾葉重)은 사천 배방사지 차나무가 생엽중(0.81 g), 건엽중(0.34 g)으로 가장 무거웠으며, 가장 작은 지역은 부산 전등사가 생엽중(0.25 g)이며, 건엽중은 전등사와 범어사(0.10 g)가 가장 가볍게 나타났다. 차나무 잎의 형질간 상관관계는 수량성을 나타내는 건엽중은 엽장, 엽폭, 엽면적, 생엽중과 고도의 유의성을 보였으며 0.8이상의 매우 높은 유의차를 보였다. 본 연구 결과 사천 배방사지 차나무가 엽장(葉長), 엽폭(葉幅), 엽면적(葉面積), 엽병장(葉柄長), 거치수(鋸齒數), 생엽무게, 건조무게 등은 전체적으로 가장 크고 무거웠으며, 엽맥수(葉脈數)는 하동 쌍계사 주변의 차나무가 가장 많았다. 자생하는 차나무의 다양한 유전적 변이를 이용하여 유용한 유전자원으로 이용한다면 우량 다수성 계통의 신품종 개발 가능성이 높을 것으로 판단되며, 금후 자생지역별로 생육환경이나 지리적 특성도 검토 분석 한다면 기능성 신품종 개발 보급에 기초 자료로서 활용성이 높을 것으로 판단된다. We collected the tea leaves from the vicinity of 14 Buddhist temple located in Busan and Gyeongnam area where wild tea trees and more than 30 year old tee trees were commonly found. The characteristics of the tea leaves were surveyed. Among those tee leaves from 14 areas, Baebangsaji (Goseong) tea leaves had the highest value of leaf length (12.04 ㎝), leaf width(4.90 cm), and leaf area (41.33 ㎝). The tea leaves of Jeondeungsa (Busan) had the lowest value of leaf length (6.30 ㎝) and leaf width (2.48 ㎝). The tea leaves of Bonggoksa (Geoje) had the lowest value of leaf area (14.80 ㎝). The longest leaf stalk length was Umgwangsaji (Miryang) tea leaves (0.57 ㎝) and Baedangsaji (Goseong) tea leaf (0.57 ㎝). The largest No. of serrate was Baedangsaji (Goseong) tea leaves (30.13) and for leaf vein was Ssanggyesa (Hadong) as a number of 11.27. The heaviest weight of fresh leaf was Baedangsaji (Goseong) tea leaf (0.81 g) and, also, the dry tea leaf of Baedangsaji (Goseong) was the heaviest (0.34 g). The lightest weight of fresh leaf was Jeondeungsa (Busan) tea leaves (0.25 g) and the lightest weight of dry leaf was Jeondeungsa. In this study, leaf length, leaf width, leaf area, leaf stalk length, No. of serrate, weight of fresh leaf and weight of dry leaf value of the leaves collected from Baedangsaji (Goseong) was generally high and The highest value of No. of leaf vein was from Ssanggyesa (Hadong).

건조 상추 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.3

상추는 가장 선호하는 녹색 채소 중 하나이다. 상추는 폴리페놀성 화합물을 비롯한 다양한 성분을 함유하고 있으며, 항균, 항산화, 항염증 등의 생리활성을 가지고 있는 것으로 알려져 있다. 본 연구에서는 건조상추의 에탄올 추출물(DLE)을 제조하고 이들의 항염증 활성을 연구하였다. DLE의 항염증 활성을 측정하기 위하여 LPS로 활성화된 마우스 대식세포 RAW 264.7 세포주에서 nitric oxide (NO) 생성을 측정하였다. DLE는 세포주의 생존에는 영향을 미치지 않으면서 NO 생산을 현저하게 저해하였다. DLE에 의해 염증 유전자인 iNOS와 COX-2의 유전자와 단백질의 발현이 모두 감소하였으며, 6개의 염증관련 cytokine 유전자(IL-1α, IL-1β, IL-1F6, TNF-α, CSF2, 그리고 CXCL10)의 발현이 모두 감소하였다. 또한, DLE의 처리는 MAPKs 경로의 인산화를 모두 저해하였으며, NF-κB p65의 핵으로의 이동을 저해하였다. 이러한 결과는 DLE의 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 시사한다. 또한, DLE는 농도의존적으로 reactive oxygen species (ROS)의 생산을 저해하였으며, hemeoxygenase-1 (HO-1) 단백질의 발현을 증가시켰으며, HO-1의 전사조절인자인 Nrf2의 핵으로의 이동을 증가시켰다. 결론적으로, 이러한 연구결과는 DLE가 염증관련 유전자의 발현을 감소시키며, MAPKs, NF-κB, 그리고 Nrf2/HO-1 등 다양한 경로를 조절함으로써 항염증 활성을 가지는 것을 제시한다. Lettuce (Lactuca sativa L.) is one of the most popular green leafy vegetables, and it contains various beneficial components including polyphenolic compounds and has been known to possess various biological functions such as anti-microbial, anti-oxidative, and anti-inflammatory activities. In the present study, we prepared ethanol extract of dried lettuce (DLE) and investigated its anti-inflammatory activity. To evaluate the anti-inflammatory activity of DLE, nitric oxide (NO) production was measured in LPS-activated mouse macrophage RAW 264.7 cells. DLE significantly suppressed NO production in these cells without affecting cell viabilities while resveratrol was used as a positive control. DLE dramatically decreased the expression of pro-inflammatory genes such as iNOS and COX-2 at the mRNA and protein levels and reduced the expression of several cytokines including IL-1α, IL-1β, IL-1F6, TNF-α, CSF2 and CXCL10. In addition, DLE suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating DLE shows its anti-inflammatory activity via regulating MAPKs pathway and NF-κB pathways. And also, DLE reduced the production of reactive oxygen species in a dose-dependent manner. DLE increased HO-1 protein expression, and also increased the nuclear translocation of Nrf2. Overall, our results suggest that lettuce down-regulate various pro-inflammatory genes and have its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

연잎, 연자육, 연자방 에탄올 추출물의 항염증 활성

이은주(Eun-Joo Lee),서유미(Yu-Mi Seo),김용현(Yong-Hyun Kim),정정욱(Chungwook Chung),성화정(Hwa-Jung Sung),손호용(Ho-Yong Sohn),박종이(Jong-Yi Park),김종식(Jong-Sik Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.4

연은 아시아 국가에서 음식과 약재로 널리 사용되는 재료이다. 본 연구에서는 연의 잎(leaf, NL), 자육(seed, NS), 자방(seedpod, NSP)으로부터 에탄올 추출물을 제조하고 이들의 항염증 활성과 작용기전을 규명하였다. 이들의 항염증 활성을 연구하기 위하여 LPS로 자극된 RAW 264.7 세포에서 nitric oxide (NO) 생산을 측정하였다. NL, NS, NSP는 세포 생존율에 영향을 주지 않으면서, 농도의존적으로 NO의 생산을 현저하게 저해하였으며, iNOS 및 COX-2와 같은 pro-inflammatory 중재자들의 단백질 발현을 감소시켰다. 또한, NL, NS, NSP는 MAPKs 단백질의 인신화를 감소시키고 NF-κB p65의 핵으로의 이동을 저해함으로써, 세 추출물에 의한 항염증 활성은 MAPKs 경로와 NF-κB 경로를 조절함으로써 이루어짐을 제시한다. 게다가, ROS의 생성이 세 추출물에 의해서 모두 저해되었으며, HO-1의 발현과 HO-1의 전사조절인자인 Nrf2의 핵으로의 이동이 증가되었다. 결론적으로, 이러한 연구결과는 연의 다양한 부위의 추출물인 NL, NS 그리고 NSP는 항염증 활성을 가지고 있으며, MAPKs, NF-κB, Nrf2/HO-1 등 다양한 신호경로를 통해 조절할 수 있음을 제시한다. Nelumbo nucifera, also known as sacred lotus, has mainly been used as a food throughout the Asian countries. In the present study, we prepared ethanol extracts from leaf (NL), seed (NS), and seedpod (NSP) of Nelumbo nucifera and investigated their anti-inflammatory activities in mouse macrophage RAW 264.7 cells. To evaluate the anti-inflammatory activities of NL, NS, and NSP, nitric oxide (NO) production was measured in LPS-stimulated RAW 264.7 cells. NL, NS, and NSP significantly reduced NO production in a dose-dependent manner without affecting cell viabilities. NL, NS, and NSP dramatically decreased the protein expression of pro-inflammatory genes such as iNOS and COX-2. NL, NS, and NSP also suppressed phosphorylation of MAPKs and the nuclear translocation of NF-κB p65 indicating they have their anti-inflammatory activities via regulating mitogen-activated protein kinases (MAPKs) and nuclear factor kappa B (NF-κB) pathways. In addition, we analyzed the production of reactive oxygen species (ROS) by the treatment of NL, NS, and NSP. All extracts reduced ROS production in a dose-dependent manner. And also, they increased heme oxygenase-1 (HO-1) protein expression and the nuclear translocation of nuclear respiratory factor 2 (Nrf2). In conclusion, our results suggest that Nelumbo nucifera has its anti-inflammatory activity via regulating MAPKs, NF-κB, and Nrf2/HO-1 pathways.

Mepivacaine-induced contraction is attenuated by endothelial nitric oxide release in isolated rat aorta

Sung, Hui-Jin,Choi, Mun-Jeoung,Ok, Seong-Ho,Lee, Soo Hee,Hwang, Il Jeong,Kim, Hee Sook,Chang, Ki Churl,Shin, Il-Woo,Lee, Heon-Keun,Park, Kyeong-Eon,Chung, Young-Kyun,Sohn, Ju-Tae Canadian Science Publishing 2012 Canadian journal of physiology and pharmacology Vol.90 No.7

<P> Mepivacaine is an aminoamide-linked local anesthetic with an intermediate duration that intrinsically produces vasoconstriction both in vivo and in vitro. The aims of this in-vitro study were to examine the direct effect of mepivacaine in isolated rat aortic rings and to determine the associated cellular mechanism with a particular focus on endothelium-derived vasodilators, which modulate vascular tone. In the aortic rings with or without endothelium, cumulative mepivacaine concentration-response curves were generated in the presence or absence of the following antagonists: N<SUP>ω</SUP>-nitro-l-arginine methyl ester [l-NAME], indomethacin, fluconazole, methylene blue, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one [ODQ], verapamil, and calcium-free Krebs solution. Mepivacaine produced vasoconstriction at low concentrations (1 × 10<SUP>−3</SUP> and 3 × 10<SUP>−3</SUP> mol/L) followed by vasodilation at a high concentration (1 × 10<SUP>−2</SUP> mol/L). The mepivacaine-induced contraction was higher in endothelium-denuded aortae than in endothelium-intact aortae. Pretreatment with l-NAME, ODQ, and methylene blue enhanced mepivacaine-induced contraction in the endothelium-intact rings, whereas fluconazole had no effect. Indomethacin slightly attenuated mepivacaine-induced contraction, whereas verapamil and calcium-free Krebs solution more strongly attenuated this contraction. The vasoconstriction induced by mepivacaine is attenuated mainly by the endothelial nitric oxide - cyclic guanosine monophosphate pathway. In addition, mepivacaine-induced contraction involves cyclooxygenase pathway activation and extracellular calcium influx via voltage-operated calcium channels. </P>