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RISS 인기검색어
- 검색키워드
- 저자 : Chuncheng Hao (검색결과 4 건)
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Hao, Wei,Tian, Pengjiao,Zheng, Mingli,Wang, Huili,Xu, Chuncheng Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1
Objective: The objective of this study was to isolate proteolytic microorganisms and evaluate their effects on proteolysis in total mixed ration (TMR) silages of soybean curd residue. Methods: TMRs were formulated with soybean curd residue, alfalfa or Leymus chinensis hay, corn meal, soybean meal, a vitamin-mineral supplement, and salt in a ratio of 25.0: 40.0:30.0:4.0:0.5:0.5, respectively, on a basis of dry matter. The microbial proteinases during ensiling were characterized, the dominate strains associated with proteolysis were identified, and their enzymatic characterization were evaluated in alfalfa (A-TMR) and Leymus chinensis (L-TMR) TMR silages containing soybean curd residue. Results: Both A-TMR and L-TMR silages were well preserved, with low pH and high lactic acid concentrations. The aerobic bacteria and yeast counts in both TMR silages decreased to about 10<sup>5</sup> cfu/g fresh matter (FM) and below the detection limit, respectively. The lactic acid bacteria count increased to 10<sup>9</sup> cfu/g FM. The total microbial proteinases activities reached their maximums during the early ensiling stage and then reduced in both TMR silages with fermentation prolonged. Metalloproteinase was the main proteinase when the total proteinases activities reached their maximums, and when ensiling terminated, metallo and serine proteinases played equally important parts in proteolysis in both TMR silages. Strains in the genera Curtobacterium and Paenibacillus were identified as the most dominant proteolytic bacteria in A-TMR and L-TMR, respectively, and both their proteinases were mainly with metalloproteinase characteristics. In the latter ensiling phase, Enterococcus faecium strains became the major sources of proteolytic enzymes in both TMR silages. Their proteinases were mainly of metallo and serine proteinases classes in this experiment. Conclusion: Proteolytic aerobic bacteria were substituted by proteolytic lactic acid bacteria during ensiling, and the microbial serine and metallo proteinases in these strains played leading roles in proteolysis in TMR silages.
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Hu, Xiaodong,Hao, Wei,Wang, Huili,Ning, Tingting,Zheng, Mingli,Xu, Chuncheng Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.4
The objective of this study was to assess the use of peach pomace in total mixed ration (TMR) silages and clarify the differences in aerobic stability between TMR and TMR silages caused by lactic acid bacteria (LAB). The TMR were prepared using peach pomace, alfalfa hay or Leymus chinensis hay, maize meal, soybean meal, cotton meal, limestone, a vitamin-mineral supplement, and salt in a ratio of 6.0:34.0:44.4:7.0:5.0:2.5:1.0:0.1 on a dry matter (DM) basis. Fermentation quality, microbial composition, and the predominant LAB were examined during ensiling and aerobic deterioration. The results indicated that the TMR silages with peach pomace were well fermented, with low pH and high lactic acid concentrations. The aerobic stability of TMR silages were significantly higher than that of TMR. Compared with TMR silages with alfalfa hay, TMR silage with Leymus chinensis hay was much more prone to deterioration. Although the dominant LAB were not identical in TMR, the same dominant species, Lactobacillus buchneri and Pediococcus acidilactici, were found in both types of TMR silages after 56 d of ensiling, and they may play an important role in the aerobic stability of TMR silages.
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Wang, Huili,Ning, Tingting,Hao, Wei,Zheng, Mingli,Xu, Chuncheng Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.1
This study investigated the dynamics associated with prolonged ensiling and aerobic deterioration of whole crop corn (WCC) silages and total mixed ration (TMR) silages containing WCC (C-TMR silages) to clarify the differences that account for the enhanced aerobic stability of TMR silages. Laboratory-scale barrel silos were randomly opened after 7, 14, 28, and 56 d of ensiling and were subjected to analyses of fermentation quality, microbial and temperature dynamics during aerobic exposure. WCC and C-TMR silages were both well preserved and microorganisms were inhibited with prolonged ensiling, including lactic acid bacteria. Yeast were inhibited to below the detection limit of 500 cfu/g fresh matter within 28 d of ensiling. Aerobic stability of both silages was enhanced with prolonged ensiling, whereas C-TMR silages were more aerobically stable than WCC silages for the same ensiling period. Besides the high moisture content, the weak aerobic stability of WCC silage is likely attributable to the higher lactic acid content and yeast count, which result from the high water-soluble carbohydrates content in WCC. After silo opening, yeast were the first to propagate and the increase in yeast levels is greater than that of other microorganisms in silages before deterioration. Besides, increased levels of aerobic bacteria were also detected before heating of WCC silages. The temperature dynamics also indicated that yeast are closely associated with the onset of the aerobic deterioration of C-TMR silage, whereas for WCC silages, besides yeast, aerobic bacteria also function in the aerobic deterioration. Therefore, the inclusion of WCC might contribute to the survival of yeast during ensiling but not influence the role of yeast in deterioration of C-TMR silages.
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Effect of branched alumina on thermal conductivity of epoxy resin
Yu Long,Lirui Shi,Qingyu Wang,Haitao Qu,Chuncheng Hao,Qingquan Lei 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-
Composites prepared by using epoxy resin (EP) as matrix had limited practical applications owing to theirpoor thermal conductivity. The thermal conductivity of composites can be enhanced by doping inorganicfillers. However, it could not be significantly enhanced owing to the saturation of inorganic fillers. In thisstudy, we prepared branched alumina (b-Al2O3) by using high-temperature sintering and doped it as afiller to obtain b-Al2O3/EP composites. The results shown that the thermal conductivity of the EP compositeswith 70 wt% b-Al2O3 was 1.13 Wm1K1, which was seven times higher than that of pure EP. Thiscould be attributed to the overlapping of b-Al2O3 for the formation of a continuous mesh structure thatresults in thermal conduction channels inside the EP, which reduced the interfacial thermal resistanceand improved the thermal conductivity of the composite. The resistivity of b-Al2O3/EP was 9.64 1014Xm and that of pure EP was 2.08 1014 Xm. The dielectric constant of the b-Al2O3/EP compositesincreased with the increase in b-Al2O3 content, while the dielectric loss factor decreases. This was significantfor the application of EP composites in the field of electrical insulation.
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