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주정 중독 흰쥐에서 총담관 결찰이 간 및 혈청의 Glyoxalase I 활성에 미치는 영향
곽춘식,김여희,변용준 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4
The activities of the rat liver and serum glyoxalase-I(GLO-I) were studied in cholestasis induced by common bile duct(CBD) ligation after chronic ethanol intoxication, and in cholestasis before acute ethanol intoxication to establish the biochemical background of alcohol hazards in hepatobiliary disease. GLO-I activities in the rat's liver and serum showed less increases when CBD was ligated after chronic ethanol intoxication than that in the CBD ligation alone. GLO-I activities in the rat's liver and serum showed no more increases when acute ethanol intoxication was induced after CBD ligation than that in the CBD ligation alone. On the other hand, when CBD was ligated after chronic ethanol intoxication, the value of Vmax of the liver GLO-I decreased significantly than that in the CBD was ligated alone. However, the values of Km of the liver GLO-I did not changes in the all experimental groups. Viewed from above results, when chronic ethanol intoxication is combined with cholestasis, GLO-I in the liver seems to be decrease its activity than that in cholestasis, and the cause of the decrease is thought to be decreased biosynthesis.
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박은동,김원규,양승학,변용준,신승화 대한산부인과학회 1995 Obstetrics & Gynecology Science Vol.38 No.9
고신의대 산부인과학교실에스는 1986년 1월부터 1994년 12월까지 진단 및 치료를 받았던 원발성 질암 20례에 대하여 임상적 고찰을 한 결과 다음과 같은 결론을 얻었다. 1. 연령별 분포는 30대에서 70대까지 분포하였으며 50-59세군이 8례(38%)로 가장 많았으며 평균나이는 54세로 나타났다. 2. 조직학적 분류상 편평세폰암이 대부분으로 85%를 차지하였고 그외 선암 2례, 편평선암 1례가 있었다. 3. 주증상은 80%의 환자에서 질출혈이었으며 그외 종괴촉지 10%, 골반통 5%, 질 분비물 5% 였다. 4. 병기별 분포를 보면 1기 1명(5%), 2기 8명(40%) 3기 2명(10%), 4기 5명(25%)였다. 5. 질병변의 위치는 상부 1/3, 그리고 후측벽에 우세하였다. 6. 치료는 수술요법, 방사선요법, 항암화학요법, 혼합요법 등을 시행하였고, 총 재발 및 지속율은 75%였고 1기에서 0%, 2기에서 63%, 3,4기에서 100%였다. 7. 전체 3년 생존율은 50%이고 1기에서 100%, 2기에서 57%, 3기에서 50%, 4기에서는 0%로 나타났다. From January 1986 until December 1994 we performed investigative clinical studies on 20 cases of primary vaginal carcinoma which comfirmed histopathologically. The results were as followings. 1. Age ranged from 30 to 70 years and the great incidence occured in 50-59 years group . 2. The most frequent histologic type is squamous cell carcinoma which was observed in 17 cases (85%), the next adenocarcinoma in 2 cases (10%) and adenosquamous cell carcinoma in 1 case(5%), respectively. 3. The most frequent chief complaint was vaginal bleeding in 16 cases (80%) and others are plapable mass in 2 cases (10%), pelvic pain in 1case (5%), vaginal dischage in 1 cases (5%), respectively. 4. The incidence of location of vaginal lesion is greatest in upper one third (60%). 5. The 3-years survival rate is 100 % in stage I, 57% in stage II, 50% in stage III, 0% in stage IV.
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유전성 비만 마우스 ( ob / ob ) 에서 Retrovirus 를 이용한 Leptin 유전자의 전입 및 발현
오상환,조무연,정인철,변용준 대한내분비학회 2000 Endocrinology and metabolism Vol.15 No.4
Background: Leptin gene is known to be related to obesity in human and animals and complete genetic defect of the gene in ob/ob mouse has been identified. Therefore, ob/ob mouse is widely used as an animal model for the study of etiology and therapy of obesity. The main biological function of leptin was thought to involve in the regulation of food intake and weight gain, however, the regulatory mechanisms by which leptin functions in the weight reduction and lowering the blood glucose level are uncertain. In the present study, retroviral-mediated leptin gene transduction into ob/ob mouse was attempted for the correction of biochemical parameters of obesity. Methods: Leptin cDNA was inserted into pLXSN retroviral vector (pLXSN-lep) and recombinant leptin expressing retrovirus particles (3 X10 CFU/mL) were produced in ?2 ecotropic packaging cells and subsequent transfection into PA317 amphotropic packaging cells. The leptin expressing recombinant viruses (LER) were transduced into NIH3T3 mouse fibroblasts and nsertion of leptin cDNA into chromosomal DNA of PA317 and MH3T3 mouse fibroblasts was confirmed by Southern blot hybridizations. Leptin mRNA and its protein expressed in the cells were identified by Northern blot hybridization and Western blot immunodetection method, respectively. LER were injected I. P. into ob/ob mice, and body weight, food intake, serum leptin level and blood glucose level were measured. Results: Expression of leptin was identified in PA317 and NIH3T3 mouse fibroblasts transduced with LER. Leptin content in sera of mice transfused with LER was drastically increased after 1 week and decreased to the almost basal level at 3 weeks after the transfusion. The body weight as well as food intake of ob/ob mouse transduced by LER decreased for the first 3 weeks and slightly increased thereafter. The reduction of both body weight and food intake in ob/ob mice transduced with LER was observed with the concomitant increase of serum leptin level, indicating that retroviral-mediated transduction of leptin gene in ob/ob mouse in vivo produced a biologically active leptin protein and released it into blood circulation. Conclusion: A transient expression of leptin cDNA in ob/ob mice by a retroviral-mediated transduction was performed and further studies are required for long term expression of the gene in vivo (J Kor Soc Endocrinol 15:502-512, 2000).
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박은동,김동휘,윤배근,변용준 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.8
본 증례는 29세 초산부가 자궁경관임신이라는 진단하에 소파술후 자궁경부결찰과 함께 질폐색을 시도하였으나 계속되는 질출혈을 일으켜, 본원으로 이송되어 Foley 도뇨관으로 자궁경관을 압박하고, metotrexate 와 folinic acid 로 치료함으로써 상태가 호전된 경우로, 자궁경관임신시 출혈정도, 임신희망여부, 임신지속 기간에 따라 우선 일차적으로 Foley 도뇨관과 methotrexate 를 이용하는 보존요법을 시행해 보는 것이 바람직하다고 생각되어 진다. Cervical pregnancy was dignosed at 9 week`s gestation in nulliparous woman presenting with vaginal bleeding. Initial treatment with cervical cerclage anad cervical packing was unsuccessful after curettage. The patient was treated by a Foley catheter wiht a 20mL balloon for intracervical tamponed,methotexate and leucovorin.
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자궁경부암 제 1 기말 , 2 기초에서 DNA배수체와 국소적 T 세포침윤의 임상조직학적 예후인자들과의 상관관계에 관한 연구
박은동,김흥열,장희경,허만하,이영미,변용준 대한산부인과학회 1994 Obstetrics & Gynecology Science Vol.37 No.1
본 연구는 자궁경부암에서 DNP ploidy 양상과 암주변부 임파구 침윤도가 이미 알려진 여러가지 임상조직학적 예후인자들과 어떠한 연관성을 갖고 있으며 새로운 예후인자로서의 유용성 여부를 알아보고자 실시하였다. 1992년 11월 1일부터 1993년 5월 30일가지 본원에 내원하여 자궁경부암 제 1기말과 2기초로 진단받고 광범위 자궁절제술 및 임파절 제거술을 받은 환자를 대상으로 하였으며 수술시 적출된 신선조직으로 DNA flowcytometry를 실시하여 DNA 양상을 분석하였고 일반 조직학적 검경 및 면역조직화학적 염색을 실시하여 임파절 전이여부를 비롯한 조직학적 소견 및 면역세포의 국소적 상대분포도를 구하여 환자의 임상소견들과 비교 검토한 결과는 다음과 같았다. 1. DNA flowcytometry를 실시한 33예중 diploid군은 19예, aneuploid군은 14에로 나타나 aneuploidy의 발생빈도는 42.4%였으며 HPP는 79%로 나타났다. 2. 자궁경부암의 임상병기 Ib에서 IIa보다 유의하게 aneuploidy의 발생빈도가 높았다(p=0.049). 3. 연령이 증가할수록 aneuploidy의 발생빈도가 어느 정도 유의하게 높은 경향을 보였다(p=0.086). 4. 임파절전이가 있는 군에서 aneuploid의 발생빈도가 매우 유의하게 높았다(p=0.02). 5. 병변의 크기, 침윤정도 및 임파혈관게 침범유무는 각각 DNA ploid양상과 유의한 연관성이 없는 것으로 나타났다. 6. 세포의 형태, 분화도, mitotic activity 및 국소적 임파구 침윤정도를 각각 DNA ploidy양상과 비교하였을때 어느 것도 유의한 연관성을 보여주지 못했다. 7. 조직학적 악성도를 세포의 형태, 분화도, mitotic activity, 국소적 임파구 침윤정도 및 임파혈관계 침범유무의 다섯가지 항목으로 점수로 나타내었을때 조직학적 악성도가 증가할수록 어느정도 유의하게 aneuploidy의 발생빈도가 높아진ㄴ 경향을 볼 수 있었다(p=0.57). 8. 암주변부 T-cell의 상대적 분포도에 따른 임과혈관계 침범유무 및 임파절 전이여부에서는 T-cell의 침윤이 상대적으로 적을수록 임파혈관계 침범은 유의하게 많았으나(p=0.007) 임파절 전이와의 유의한 연관성은 나타나지 않았다. 이상의 결과로 미루어 볼 때 자궁경부암의 예후인자로 임상적 소견과 조직학적 소견과 더불어 DNA flowcytometry에 의한 DNA양상 및 immunohistochemical stain을 통한 국소적 임파구, 특히 T-cell의 침윤정도가 자궁경부암의 악성도와 치료방침의 결정 및 예후를 판정하는데 도움이 될 것으로 사료되는 바이다. 그러나 본 연구는 전향적 조사로서 계속해서 많은 예를 모으고 충분한 시간이 경과된다면 생존율의 비교를 통해 보다 확실한 결론을 얻을 수 있으리라 기대되며 자궁경부암의 에후판정에 보다 빠르고 쉬우며 정확하고 객관적이 지표를 제시할 수 있을것으로 사료된다. In a prospective study of 33 patients with the uterine cervical cancer stage Ib, IIa, the prognostic value of DNA ploidy was evaluated in relation to the clinicopathologic variables. And we studied degree of stromal T-cell infiltration in the periphery of tumor lesion in 43 patients via immunohistochemical stain. Then, its prognostic impact was evaluated in relation to lymph node metastasis and lymphovascular invasion. The subjects were patients who admitted to Kosin medical college under the diagnosis of cervical cancer and undertaken radical hysterectomy and pelvic node dissection from Nov., 1992 to May, 1993. The samples for DNA flowcytometry were obtained from the fresh surgical specimen and sent for laboratory. Via H-E stain and immunohistochemical stain, one pathologist inspected general histologic findings and degree of T-cell, B-cell stromal infiltration. Clinicopathologic variables included age, stage, lymph node metastasis, tumor size, invasion depth, cell type, degree of differentiation, mitosis, lymphovascular invasion, degree of lymphocyte infiltration. The results were as follows: 1. The incidence of aneuploidy was 42.4% and the incidence of high proliferative phase (HPP) was 79%. 2. The incidence of aneuploidy was significantly increased in stage Ib than stage IIa (P=0.049). 3. Increasing age the incidence of aneuploidy was increased (P=0.086). 4. In the group of lymph node metastasis, incidence of aneuploidy was significantly increased(P=0.02). 5. There were no significant relationships between DNA ploidy and tumor size, invasion depth, lymphovascular invision. 6. There were no significant relationships between DNA ploidy and the cell type, degree of differentiation, mitotic activity, degree of lymphocyte infiltration. 7. According to MSG including cell type, mitotic activity, degree of stromal lymphocyte infiltration and lymphovascular invasion, the higher MSG, the higher incidence of aneuploidy (P=0.057). 8. In the study of stromal T-cell infiltration, the lower infiltration of T-cell in the periphery of the tumor, the higher incidence of lymphovascular invasion(P=0.007). And there was no significant relationship between T-cell infiltration and lymph node metastasis. From the above results it is considered that DNA ploidy and stromal T-cell infiltration are heplful additative prognostic factors with clinicipathologic characters in the cervical carcinoma.
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