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( Zhi-ke Liu ),( Qiu-yu Zhang ),( Ning-ning Yang ),( Ming-guo Xu ),( Jin-feng Xu ),( Ming-long Jing ),( Wen-xing Wu ),( Ya-dong Lu ),( Feng Shi ),( Chuang-fu Chen ) 한국미생물생명공학회(구 한국산업미생물학회) 2019 Journal of microbiology and biotechnology Vol.29 No.3
Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect Salmonella in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect Salmonella. LAMP primers were designed to target the Salmonella invA gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, MgSO<sub>4</sub> concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six Salmonella species and six related non-Salmonella strains. All six of the Salmonella strains, but none of the non-Salmonella strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of Salmonella enterica subsp. enterica serovar Pullorum genomic DNA as low as 89 fg/μl, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of Salmonella infection in chickens.
Optimization of biodiesel production from trap grease via acid catalysis
Jin-Suk Lee,Zhong-Ming Wang,Ji-Yeon Park,Chuang-Zhi Wu,Zhen-Hong Yuan 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.4
As a kind of waste collected from restaurants, trap grease is a chemically challenging feedstock for biodiesel production for its high free fatty acid (FFA) content. A central composite design was used to evaluate the effect of methanol quantity, acid concentration and reaction time on the synthesis of biodiesel from the trap grease with 50% free fatty acid, while the reaction temperature was selected at 95 oC. Using response surface methodology, a quadratic polynomial equation was obtained for ester content by multiple regression analysis. Verification experiments confirmed the validity of the predicted model. To achieve the highest ester content of crude biodiesel (89.67%), the critical values of the three variables were 35.00 (methanol-to-oil molar ratio), 11.27 wt% (catalyst concentration based on trap grease) and 4.59 h (reaction time). The crude biodiesel could be purified by a second distillation to meet the requirement of biodiesel specification of Korea.
Tie Jun Wang,Chen Guang Wang,Tie Jun Wang,Yan Gao,Chuang Zhi Wu,Long Long Ma 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.4
With naphthalene as biomass tar model compound, partial oxidation reforming (with addition of O2) and dry reforming of biomass fuel gas were investigated over nickel-based monoliths at the same conditions. The results showed that both processes had excellent performance in upgrading biomass raw fuel gas. Above 99% of naphthalene was converted into synthesis gases (H2+CO). About 2.8 wt% of coke deposition was detected on the catalyst surface for dry reforming process at 750 oC during 108 h lifetime test. However, no coke deposition was detected for partial oxidation reforming process, which indicated that addition of O2 can effectively prohibit the coke formation. O2 can also increase the CH4 conversion and H2/CO ratio of the producer gas. The average conversion of CH4 in dry and partial oxidation reforming process was 92% and 95%, respectively. The average H2/CO ratio increased from 0.95 to 1.1 with the addition of O2, which was suitable to be used as synthesis gas for dimethyl ether (DME) synthesis.