Identification of Non-Muscle Nebulin Isoform in Human Brain Library

Young Mi Joo,Min-A Lee,Pyung-Rak Choi,Jae-Kyoung Choi,Yeong-Mi Lee,Su-Il Choi,Myong-Shin Kim,Eun-Hee Jeon,So-Young Kim,Chong-Rak Kim 대한의생명과학회 2004 Biomedical Science Letters Vol.10 No.1

Nebulin is a (Mr 600~900 kDa) large actin-binding protein specific to skeletal muscle and thought to act as a molecular template that regulates the length of thin filaments. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Recently, full-length nebulin mRNA transcripts have been detected in heart muscle, but at lower levels than in skeletal muscle. Nebulin expression also was detected in the kidney, eye, and otic canal, suggesting that nebulin isoforms may also be expressed in these organs. We have searched for nebulin isoforms in brain of human using PCR and Northern blot. Here, we provide evidence that nebulin mRNA transcripts are expressed in brain. Seven nebulin isoforms (B, C, D, E, F, G and H form) are obtained in human skeletal muscle and four isoforms (B, C, G and H form) in human brain cDNA library. We cloned the 1.3 kb of nebulin fragment from human adult brain library by PCR. The identity of the PCR product was confirmed by sequence analysis. The partial brain nebulin sequence was 99% identical to the skeletal muscle cDNA as determined by Blast alignment. It contains two simple-repeats HR1, HR2 and linker-repeats exon 135~143 except exon 140. It was different from skeletal muscle B form, which contain HR1 and HR8. These data suggest that nebulin isoform diversity occurs even more extensively than previously known, likely contributing to the distinct thin filament architecture of different striated muscles.

Identification of Non-Muscle Nebulin Isoform in Human Brain Library

Joo, Young Mi,Lee, Min-A,Choi, Pyung-Rak,Choi, Jae-Kyoung,Lee, Yeong-Mi,Choi, Su-Il,Kim, Myong-Shin,Jeon, Eun-Hee,Kim, So-Young,Kim, Chong-Rak THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.1

Nebulin is a (Mr 600~900 kDa) large actin-binding protein specific to skeletal muscle and thought to act as a molecular template that regulates the length of thin filaments. Cardiac muscles of higher vertebrates have been shown earlier to lack nebulin. Recently, full-length nebulin mRNA transcripts have been detected in heart muscle, but at lower levels than in skeletal muscle. Nebulin expression also was detected in the kidney, eye, and otic canal, suggesting that nebulin isoforms may also be expressed in these organs. We have searched for nebulin isoforms in brain of human using PCR and Northern blot. Here, we provide evidence that nebulin mRNA transcripts are expressed in brain. Seven nebulin isoforms (B, C, D, E, F, G and H form) are obtained in human skeletal muscle and four isoforms (B, C, G and H form) in human brain cDNA library. We cloned the 1.3 kb of nebulin fragment from human adult bran library by PCR. The identity of the PCR product was confirmed by sequence analysis. The partial brain nebulin sequence was 99% identical to the skeletal muscle cDNA as determined by Blast alignment. It contains two simple-repeats HR1, HR2 and linker-repeals exon 135~143 except exon 140. It was different from skeletal muscle B form, which contain HR1 and HR8. These data suggest that nebulin isoform diversity occurs even more extensively than previously known, likely contributing to the distinct thin filament architecture of different striated muscles.

FET형 반도체센서 및 시스템 개발

손병기,조진호,최평,박이순,서화일,권대혁,고광락 경북대학교 센서기술연구소 1996 연차보고서 Vol.1996 No.-

기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질(electrolyte :H^+, K^+, Ca^2+, Na^+)센서소자 및 분석 시스템의 개발을 중점적으로 추진하였으며, FET형 포도당센서 및 압력센서의 개별 FET형 센서에 관한 연구도 병행하였다. FET형 전해질센서를 이용한 휴대용 전해질 측정기와 desktop형 4채널 전해질 분석기를 제작하였다. 또한 이 시스템을 소형화하기 위한 주문형 아날로그-디지탈 변환기 내장형 CMOS 프로세서를 설계하고 검증하였다. The main object of this research is to develope a new FET type electrolyte(H^+, K^+, Ca^2+, Na^+) sensors and analysis system which can overcome the problems of the conventional sensors. Parallel researches on FET type sensors such as glucose and pressure humidity are also in progress. A portable electrolyte meter and desktop 4-channel electrolyte analysis system is fabricated. A customized CMOS processor with built-in analog-to-digital converter is designed and verified.

Identification of Differentially Expressed Genes in Human Small Cell Lung Carcinoma Using Subtractive Hybridization

Ahn Seung-Ju,Choi Jae-Kyoung,Joo Young Mi,Lee Min-A,Choi Pyung-Rak,Lee Yeong-Mi,Kim Myong-Shin,Kim So-Young,Jeon Eun-Hee,Min Byung-In,Kim Chong-Rak 대한의생명과학회 2004 Biomedical Science Letters Vol.10 No.3

Lung cancer is a leading cause of cancer death worldwide; however, despite major advances in cancer treatment during the past two decades, the prognostic outcome of lung cancer patients has improved only minimally. This is largely due to the inadequacy of the traditional screening approach of diagnosis in lung cancer, which detects only well­established overt cancers and fails to identify precursor lesions in premalignant conditions of the bronchial tree. In recent years this situation has fundamentally changed with the identification of molecular abnormalities characteristic of premalignant changes; these concern tumour suppressor genes, loss of heterozygosity at crucial sites and activation of oncogenes. Basic knowledge at the molecular level has extremely important clinical implications with regard to early diagnosis, risk assessment and prevention, and therapeutic targets. In this study we used a 'cap-finder' subtractive hybridization method, 'long distance' polymerase chain reaction (PCR), streptavidin magnetic beads mediated subtraction, and spin column chromatography to detect differential expression genes of human small cell lung carcinoma. We have now isolated ninety two genes that expressed differentially in the human small cell lung carcinoma cells and analyzed of 12 clones with sequencing, nine cDNAs include tapasin (NGS-17) mRNA, BC200 alpha scRNA, chromosome 12q24 PAC RPCI3-462E2, protein phosphatase 1 (PPPICA), translocation protein 1 (TLOC1), ribosomal protein S24 (RPS24) mRNA, protein phosphatase (PPEF2), cathepsin Z, MDM2 gene and three novel genes. They may be oncogenesis­related proteins.

Identification of Differentially Expressed Genes in Human Small Cell Lung Carcinoma Using Subtractive Hybridization

Ahn, Seung-Ju,Choi, Jae-Kyoung,Joo, Young Mi,Lee, Min-A,Choi, Pyung-Rak,Lee, Yeong-Mi,Kim, Myong-Shin,KIm, So-Young,Jeon, Eun-Hee,Min, Byung-In,Kim, Chong-Rak THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2004 Journal of biomedical laboratory sciences Vol.10 No.3

Lung cancer is a leading cause of cancer death worldwide; however, despite major advances in cancer treatment during the past two decades, the prognostic outcome of lung cancer patients has improved only minimally. This is largely due to the inadequacy of the traditional screening approach of diagnosis in lung cancer, which detects only well-established overt cancers and fails to identify precursor lesions in premalignant conditions of the bronchal tree. In recent years this situation has fundamentally changed with the identification of molecular abnormalities characteristic of premalignant changes; these concern tumour suppressor genes, loss of heterozygosity at crucial sites and activation of oncogenes. Basic knowledge at the molecular level has extremely important chical implications with regard to early diagnosis, risk assessment and prevention, and therapeutic targets. In this study we used a "cap-finder" subtractive hybndization method, "long distance" polymerase chain reaction (PCR), streptavidin magnetic beads mediated subtraction, and spin column chromatography to detect differential expression genes of human small cell lung carcinoma. We have now isolated ninety two genes that expressed differentially in the human small cell lung carcinoma cells and analyzed of 12 clones with sequencing, nine cDNAs include tapasin (NGS-17) mRNA, BC200 alpha scRNA, chromosome 12q24 PAC RPC13-462E2, protein phosphatase 1 (PPPICA), translocation protein 1 (TLOCl), ribosomal protein S24 (WS24) mRNA, protein phosphatase (PPEF2), cathepsin Z, MDM2 gene and three novel genes. They may be oncogenesis-related proteins.

Identification of Differentially Expressed Genes in Human Small Cell Lung Carcinoma Using Subtractive Hybridization

Seung-Ju Ahn,Jae-Kyoung Choi,Young Mi Joo,Min-A Lee,Pyung-Rak Choi,Yeong-Mi Lee,Myong-Shin Kim,So-Young Kim,Eun-Hee Jeon,Byung-In Min,Chong-Rak Kim 대한의생명과학회 2004 Biomedical Science Letters Vol.10 No.3

Lung cancer is a leading cause of cancer death worldwide; however, despite major advances in cancer treatment during the past two decades, the prognostic outcome of lung cancer patients has improved only minimally. This is largely due to the inadequacy of the traditional screening approach of diagnosis in lung cancer, which detects only well-established overt cancers and fails to identify precursor lesions in premalignant conditions of the bronchial tree. In recent years this situation has fundamentally changed with the identification of molecular abnormalities characteristic of premalignant changes; these concern tumour suppressor genes, loss of heterozygosity at crucial sites and activation of oncogenes. Basic knowledge at the molecular level has extremely important clinical implications with regard to early diagnosis, risk assessment and prevention, and therapeutic targets. In this study we used a "cap-finder" subtractive hybridization method, "long distance" polymerase chain reaction (PCR), streptavidin magnetic beads mediated subtraction, and spin column chromatography to detect differential expression genes of human small cell lung carcinoma. We have now isolated ninety two genes that expressed differentially in the human small cell lung carcinoma cells and analyzed of 12 clones with sequencing, nine cDNAs include tapasin (NGS-17) mRNA, BC200 alpha scRNA, chromosome 12q24 PAC RPCI3-462E2, protein phosphatase 1 (PPPICA), translocation protein 1 (TLOC1), ribosomal protein S24 (RPS24) mRNA, protein phosphatase (PPEF2), cathepsin Z, MDM2 gene and three novel genes. They may be oncogenesis-related proteins.

Characteristics of Vitrification Process and Vitrified Form for Radioactive Waste

Kim, Cheon-Woo,Kim, Ji-Yean,ChoI, Jong-Rak,Ji, Pyung-Kook,Park, Jong-Kil,Shin, Sang-Woon,Ha, Jong-Hyun,Song, Myung-Jae Korean Radioactive Waste Society 2004 방사성폐기물학회지 Vol.2 No.3

In order to vitrify the combustible dry active waste (DAW) generated from Korean Nuclear Power Plants, a glass formulation development based on waste composition was performed. A borosilicate glass, DG-2, was formulated to vitrify the DAW in an induction cold crucible melter (CCM). The processability, product performance, and volume reduction effect of the candidate glass were evaluated using a computer code and were measured experimentally in the laboratory and CCM. The glass viscosity and electrical conductivity as the process parameters were in the desired ranges. Start-up and maintaining glass melt of the candidate glass were favorable in the CCM. The product of the glass product such as chemical durability, phase stability, and density was satisfactory. The vitrification process using the candidate glass was also evaluated assuming that it was operated as economically as possible.

Characteristics of Vitrification Process and Vitrified Form for Radioactive Waste

Cheon-Woo Kim,Ji-Yean Kim,Jong-Rak Choi,Pyung-Kook Ji,Jong-Kil Park,Sang-Woon Shin,Jong-Hyun Ha,Myung-Jae Song 한국방사성폐기물학회 2004 방사성폐기물학회지 Vol.2 No.3

In order to vitrify the combustible dry active waste (DAW) generated from Korean Nuclear Power Plants, a glass formulation development based on waste composition was performed. A borosilicate glass, DG-2, was formulated to vitrify the DAW in an induction cold crucible melter (CCM). The processability, product performance, and volume reduction effect of the candidate glass were evaluated using a computer code and were measured experimentally in the laboratory and CCM. The glass viscosity and electrical conductivity as the process parameters were in the desired ranges. Start-up and maintaining glass melt of the candidate glass were favorable in the CCM. The product of the glass product such as chemical durability, phase stability, and density was satisfactory. The vitrification process using the candidate glass was also evaluated assuming that it was operated as economically as possible.

Common Traffic Channel Access Method of the Third Generation Packet Service System

Kang Won Lee,Kwang Ho Kook,Pyung Joong Song,Nam Hee Lee,Sun Bae Lim,Jeong Rak Choi 한국경영과학회 1999 한국경영과학회 학술대회논문집 Vol.- No.2

FET형 반도체센서 및 시스템 개발(Ⅱ)

손병기,박이순,고광락,김창수,조진호,서장수,김명남,최 평,서희돈,이준하 경북대학교 센서기술연구소 1997 연차보고서 Vol.1997 No.-

현재 여러 가지 형태의 센서들이 고유의 용도에 맞게 연구 개발되어 사용되고 있으나, 측정환경에 영향을 주지 않을 만큼 충분히 작은 센서로 현재의 상태를 현시적으로 측정해야 하는 상황에서는 이들을 적용하기에 많은 어려움이 있었다. 그러나, FET형 반도체센서는 집적회로 제조공정을 활용하여 제조되므로 소형화, 규격화 및 양산화가 가능할 뿐만 아니라 현장 현시적 모니터링에 유리하다. 또한 측정회로를 함께 집적시킨 스마트센서 및 여러 가지 대상량을 동시에 측정할 수 있는 다기능센서에 매우 적합한 형태의 센서이다. 특히 FET의 게이트 상에 측정하고자 하는 각종 물리량이나 화학량에 감응하는 물질을 형성함으로써 여러 분야에 그 활용폭이 광범위하다. 현재 의료진단, 화학공정의 모니터링이나 환경공학적 감시 및 제어 등의 분야에서 사용되고 있는 기존의 센서는 고가이며 용적이 클 뿐만 아니라 분석시간이 길고 사용하기 까다로운 것 등 여러 가지 문제점이 있다. 또한 측정환경에 영향을 주지 않을 만큼 충분히 작으며 빠른 분석시간을 가진 센서를 필요로 하고 있다. 본 연구에서는 기존의 센서들의 난점을 극복할 수 있는 새로운 형태의 FET형 전해질 (electrolyte : H^+, K^+, Ca^2+, Na^+)센서소자 및 분석 시스템의 개발을 중점적으로 추진하였으며, 센서시스템 집적화 및 생체정보 전송용 텔레미트리 시스템의 ASIC화 관한 연구도 병행하였다. Recently various kinds of sensors have been developed, being applicated to their own purpose. There are lots of difficulties to apply them to measurements in which the real-time monitoring is required without disturbing the surrounding environment. FET type semiconductor sensors, fabricated by the semiconductor integration technologies, have many advantages for their miniaturization, standardization, mass-production and in vivo/in situ monitoring. They also hold a very proper configuration for multi-functional sensors or integrated smart sensors, and wide availability by forming various kinds of physical or chemical sensing materials onto their sensing gates. The conventional sensors have many problems such as high cost, large dimension, long analysis time and troublesome handling to apply to the fields of medical diagnosis, monitoring of chemical process and environmental monitoring/control. The main object of this research are to develope a new FET type electrolyte (H^+, K^+, Ca^2+, Na^+) sensors and analysis system which can overcome the problems of the conventional sensors, and parallel integration of a sensor system and ASIC of a telemetry system for physiological signal are also in progress.