http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
John Hwa Lee,Yeong Hwan Choi,Min Su Choi,Jin Hur 한국예방수의학회 2013 예방수의학회지 Vol.37 No.4
We previously developed a novel attenuated Salmonella Typhimurium (S.Typhimurium) △lon△cpxR vaccine. This study was conducted in order to examine whether this vaccine could effectively protect growing piglets against Salmonella infection. Pregnant sows in group A were primed and boosted with the vaccine, whereas pregnant sows in group B received sterile PBS-sucrose. After farrowing, newborn piglets in groups A and B were challenged with a wild type virulent S. Typhimurium at three weeks of age. During the study, serum IgG titers of piglets in group A were significantly higher than those of piglets in group B (P<0.001). In addition, clinical signs were observed in 5.9% of piglets in group A during the entire experimental period after the challenge, while diarrhea was observed in 81.6% of piglets in group B. These results indicate that vaccination of the pregnant sows resulted in effective protection in piglets against Salmonella infection.
Short Communications : Efficacy of two virulence gene-deleted Salmonella
( John Hwa Lee ),( Yeong Hwan Choi ),( Min Su Choi ),( Jin Hur ) 한국예방수의학회 2013 예방수의학회지 Vol.37 No.4
We previously developed a novel attenuated Salmonella Typhimurium (S.Typhimurium) △lon△cpxR vaccine. This study was conducted in order to examine whether this vaccine could effectively protect growing piglets against Salmonella infection. Pregnant sows in group A were primed and boosted with the vaccine, whereas pregnant sows in group B received sterile PBS-sucrose. After farrowing, newborn piglets in groups A and B were challenged with a wild type virulent S. Typhimurium at three weeks of age. During the study, serum IgG titers of piglets in group A were significantly higher than those of piglets in group B (P<0.001). In addition, clinical signs were observed in 5.9% of piglets in group A during the entire experimental period after the challenge, while diarrhea was observed in 81.6% of piglets in group B. These results indicate that vaccination of the pregnant sows resulted in effective protection in piglets against Salmonella infection.
Assessment of Great Vessels for Anterior Access of L5/S1 Using Patient Positioning
Choi John,Rhee Isaac,Ruparel Sameer 대한척추외과학회 2020 Asian Spine Journal Vol.14 No.4
Study Design: This was a prospective cross-sectional study.Purpose: The aim was to describe the effect of patient positioning, from supine to lateral decubitus position, on the width of the L5/S1 anterior disk space defined by the great vessels.Overview of Literature: The application of the lateral decubitus position interbody fusion has been rapidly increasing; however, there are concerns regarding the access to the lumbosacral region due to the great vessels, which necessitates further morphometric data.Methods: A total of 20 consecutive live patients awaiting lumbar surgery were subjected to two magnetic resonance imaging scans on the same day in both supine and lateral decubitus positions at a single center to investigate the anterior L5/S1 disk space.Results: The bare anterior L5/S1 disk window was present in all patients of this study population, and the mean width was 27 mm in the supine and 22 mm in the lateral decubitus position, with a mean reduction of 5.2 mm between the positions. The oblique corridor angle was measured at a mean of 33°.Conclusions: The bare window of L5/S1 disk space was present within this population group, and it was found to be mobile and changed significantly with patient positioning. Therefore, the spine surgeon or the access surgeon must consider the increased potential vascular risk during disk access in lateral decubitus anterior lumbar interbody spinal fusion surgery.
Mutations of <i>ADAMTS9</i> Cause Nephronophthisis-Related Ciliopathy
Choi, Yo Jun,Halbritter, Jan,Braun, Daniela A.,Schueler, Markus,Schapiro, David,Rim, John Hoon,Nandadasa, Sumeda,Choi, Won-il,Widmeier, Eugen,Shril, Shirlee,Kö,rber, Friederike,Sethi, Sidharth K. Elsevier 2019 American journal of human genetics Vol.104 No.1
<P>Nephronophthisis-related ciliopathies (NPHP-RCs) are a group of inherited diseases that are associated with defects in primary cilium structure and function. To identify genes mutated in NPHP-RC, we performed homozygosity mapping and whole-exome sequencing for >100 individuals, some of whom were single affected individuals born to consanguineous parents and some of whom were siblings of indexes who were also affected by NPHP-RC. We then performed high-throughput exon sequencing in a worldwide cohort of 800 additional families affected by NPHP-RC. We identified two <I>ADAMTS9</I> mutations (c.4575_4576del [p.Gln1525Hisfs<SUP>∗</SUP>60] and c.194C>G [p.Thr65Arg]) that appear to cause NPHP-RC. Although ADAMTS9 is known to be a secreted extracellular metalloproteinase, we found that ADAMTS9 localized near the basal bodies of primary cilia in the cytoplasm. Heterologously expressed wild-type ADAMTS9, in contrast to mutant proteins detected in individuals with NPHP-RC, localized to the vicinity of the basal body. Loss of ADAMTS9 resulted in shortened cilia and defective sonic hedgehog signaling. Knockout of <I>Adamts9</I> in IMCD3 cells, followed by spheroid induction, resulted in defective lumen formation, which was rescued by an overexpression of wild-type, but not of mutant, ADAMTS9. Knockdown of <I>adamts9</I> in zebrafish recapitulated NPHP-RC phenotypes, including renal cysts and hydrocephalus. These findings suggest that the identified mutations in <I>ADAMTS9</I> cause NPHP-RC and that ADAMTS9 is required for the formation and function of primary cilia.</P>
Dynamic Microcontainers as Microvacuums for Collecting Nanomaterials After Clinical Treatments
Choi, Daniel S.,Park, Jungrae,Ke Xu,Kringel, Rose,Choi, John J.,Jeon, In Tak,Young Keun Kim IEEE 2013 IEEE transactions on magnetics Vol.49 No.7
<P>We present a feasible method to collect ferromagnetic nanomaterials(FNMs) after clinical utilization by employing ferromagnetic microcontainers (MCs). The cubic MCs with dimensions of 200 micrometers have gold-coated nickel frames and were tethered such a way that they are able to remove FNMs from cells with the use of an external magnetic field. The study has been conducted in two parts: 1) enhancement of the motion of MCs in glass-based microfluidic channels filled with viscous fluids by magnetically-driven spinning MCs, i.e., “dynamic MCs”; 2)sweeping FNMs from the cells using magnetic attractive forces between FNMs and MCs through a “microvacuum”process. Our study shows that spinning MCs can transport better than nonspinning MCs through viscous fluids. We found that approximately 70% of FNMs internalized with human embryonic cells (HEK-293) were removed from the cells by the spinning MCs. Such in-vitro experiments suggest the possibility of resolving the issue of removing FNMs used for clinical treatments from human body after treatments.</P>
Multilocus sequence typing analysis of Shigella flexneri isolates collected in Asian countries
Choi, Seon Young,Jeon, Yoon-Seong,Lee, Je Hee,Choi, Boram,Moon, Sun Hwa,von Seidlein, Lorenz,Clemens, John D.,Dougan, Gordon,Wain, John,Yu, Jun,Lee, Je Chul,Seol, Sung Yong,Lee, Bok Kwon,Song, Jae-Hoo Microbiology Society 2007 Journal of medical microbiology Vol.56 No.11
Choi, Jung-Hyun,Jung, Byung-Ha,Kang, Ok-Hwa,Choi, Hyuk Joon,Park, Pil Sang,Cho, Sun Hee,Kim, Youn-Chul,Sohn, Dong Hwan,Park, Hyun,Lee, John Hwa,Kwon, Dong-Yeul Pharmaceutical Society of Japan 2006 Biological & pharmaceutical bulletin Vol.29 No.5
<P>The anti-inflammatory and anti-nociceptive effects and sedative activities of the ethyl acetate fraction of <I>Cynanchum paniculatum</I> (EACP) were evaluated in mice and rats by acetic acid-induced vascular permeability, arachidonic acid-induced paw edema, cotton pellet-induced granuloma formation, formalin-induced licking time, acetic acid-induced writhing response, and pentobarbital-induced sleeping time. EACP at a dose of 40 mg/kg significantly exhibited anti-inflammatory activities on acetic acid-induced vascular permeability, arachidonic acid-induced paw edema, and the late phase of formalin-induced licking time. Moreover, it showed anti-nociceptive effects on acetic acid-induced writhing responses and significant sedative effects on pentobarbital-induced sleeping time. The results demonstrated that the anti-nociceptive effects are apparently related to the sedative effects of EACP. These results support the use of <I>Cynanchum paniculatum</I> in relieving inflammatory pain.</P>